scholarly journals Whole bone marrow cell culture: A convenient protocol for the in vitro expansion of endothelial progenitor cells

2014 ◽  
Vol 8 (3) ◽  
pp. 805-812 ◽  
Author(s):  
JUN-FENG LIU ◽  
ZHONG-DONG DU ◽  
ZHI CHEN ◽  
ZHI-XU HE
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Endothelial Progenitor ◽  
In Vitro Expansion ◽  
Bone Marrow Cell Culture

P-201 Severe hypoxia promotes selection of repopulating progenitor cells in primary MDS bone marrow cell culture

2013 ◽  
Vol 37 ◽  
pp. S114
Author(s):  
E. Masala ◽  
F. Buchi ◽  
A. Valencia-Martinez ◽  
A. Gozzini ◽  
A. Sanna ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Severe Hypoxia ◽  
Bone Marrow Cell Culture ◽  
Selection Of

CYTOKINE REQUIREMENT FOR THE GM-CSF INDEPENDENT DEVELOPMENT OF RAT DENDRITIC CELLS (DC) BY IN VITRO BONE MARROW CELL CULTURE.

2000 ◽  
Vol 69 (Supplement) ◽  
pp. S251
Author(s):  
Hua Yan ◽  
Tohko Miyagi ◽  
Hirohisa Saito ◽  
Daniel P. Gold ◽  
Xiao-K. Li ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Dendritic Cells ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Gm Csf ◽  
Bone Marrow Cell Culture

In vitro bone marrow cell culture and cytogenetic analysis in a case of myelodysplasia

1991 ◽  
Vol 56 (2) ◽  
pp. 203-207 ◽  
Author(s):  
Adriana Maccari ◽  
Giovanna Venti Donti ◽  
Maria T. Rizzo ◽  
Rita Paltriccia ◽  
Antonio Tabilio ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Bone Marrow Cell ◽  
Cytogenetic Analysis ◽  
Marrow Cell ◽  
Bone Marrow Cell Culture

scholarly journals Radio-detoxified LPS alters bone marrow-derived extracellular vesicles and endothelial progenitor cells

2019 ◽  
Vol 10 (1) ◽  
Author(s):  
Hargita Hegyesi ◽  
Nikolett Sándor ◽  
Géza Sáfrány ◽  
Virág Lovas ◽  
Árpád Kovács ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Extracellular Vesicles ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Tumor Therapy ◽  
Mass Spectrometry Analysis ◽  
Endothelial Progenitor ◽  
Lps Treatment

Abstract Stem cell-based therapies raise hope for cell replacement and provide opportunity for cardiac regenerative medicine and tumor therapy. Extracellular vesicles are a membrane-enclosed intercellular delivery system with the potential to improve the therapeutic efficacy of the treatment of a variety of disorders. As the incidence of breast cancer continues to rise, radiotherapy has emerged as a leading treatment modality. Radiotherapy also increases the risk of coronary heart disease and cardiac mortality. In a chest-irradiated mouse model of cardiac injury, we investigated the effects of local irradiation. We found an increased lethality after 16 Gy irradiation. Importantly, radio-detoxified LPS (RD-LPS) treatment prolonged the survival significantly. By flow cytometry, we demonstrated that upon administration of RD-LPS, the number of bone marrow-derived endothelial progenitor cells increased in the bone marrow and, in particular, in the circulation. Furthermore, mass spectrometry analysis showed that RD-LPS altered the proteomic composition of bone marrow cell-derived small extracellular vesicles (sEVs). RD-LPS treatment increased interferon-induced transmembrane protein-3 (IFITM3) expression markedly both in bone marrow cells and in bone marrow cell-derived small extracellular vesicles. This is the first study to demonstrate that radio-detoxified LPS treatment induces an increase of circulating endothelial progenitor cells (EPCs) in parallel with a reduced radiotherapy-related mortality. While the total number of bone marrow-derived extracellular vesicles was significantly increased 24 h after treatment in the RD-LPS groups, the number of endothelial progenitor cells was reduced in animals injected with GW4896 (a chemical inhibitor of exosome biogenesis) as compared with controls. In contrast to these in vivo results, in vitro experiments did not support the effect of sEVs on EPCs. Our data raise the intriguing possibility that IFITM3 may serve as a marker of the radio-detoxified LPS treatment.

79 Selection by severe hypoxia of repopulating progenitor cells in primary MDS bone marrow cell culture

2011 ◽  
Vol 35 ◽  
pp. S30
Author(s):  
E. Masala ◽  
E. Spinelli ◽  
F. Buchi ◽  
A. Valencia-Martinez ◽  
A. Gozzini ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Severe Hypoxia ◽  
Bone Marrow Cell Culture

75 REPOPULATING PROGENITOR CELLS IN PRIMARY MDS BONE MARROW CELL CULTURE IN SEVERE HYPOXIC CONDITIONS

2015 ◽  
Vol 39 ◽  
pp. S36-S37
Author(s):  
E. Masala ◽  
F. Buchi ◽  
S. Pillozzi ◽  
A. Valencia Martinez ◽  
T. Rondelli ◽  
...  
Keyword(s):  
Cell Culture ◽  
Bone Marrow ◽  
Progenitor Cells ◽  
Bone Marrow Cell ◽  
Marrow Cell ◽  
Hypoxic Conditions ◽  
Bone Marrow Cell Culture
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