Ionizing radiation-induced apoptosis in human lymphoma cell lines differing in p53 status.

2000 ◽  
Author(s):  
Y Ogawa ◽  
A Nishioka ◽  
T Inomata ◽  
S Kataoka ◽  
K Nakayama ◽  
...  
Keyword(s):  
Ionizing Radiation ◽  
Cell Lines ◽  
Lymphoma Cell ◽  
Human Lymphoma ◽  
Radiation Induced ◽  
P53 Status ◽  
Induced Apoptosis

scholarly journals Sulfation of keratan sulfate proteoglycan reduces radiation-induced apoptosis in human Burkitt's lymphoma cell lines

FEBS Letters ◽  
2012 ◽  
Vol 587 (2) ◽  
pp. 231-237 ◽  
Author(s):  
Fumiaki Nakayama ◽  
Sachiko Umeda ◽  
Tomomi Ichimiya ◽  
Shin Kamiyama ◽  
Masaharu Hazawa ◽  
...  
Keyword(s):  
Cell Lines ◽  
Keratan Sulfate ◽  
Burkitt’S Lymphoma ◽  
Lymphoma Cell ◽  
Burkitt's Lymphoma ◽  
Sulfate Proteoglycan ◽  
Radiation Induced ◽  
Induced Apoptosis

scholarly journals Differential Effects of Gold Nanoparticles and Ionizing Radiation on Cell Motility between Primary Human Colonic and Melanocytic Cells and Their Cancerous Counterparts

2021 ◽  
Vol 22 (3) ◽  
pp. 1418
Author(s):  
Elham Shahhoseini ◽  
Masao Nakayama ◽  
Terrence J. Piva ◽  
Moshi Geso
Keyword(s):  
Gold Nanoparticles ◽  
Ionizing Radiation ◽  
Cancer Cells ◽  
Cell Lines ◽  
Colorectal Adenocarcinoma ◽  
X Rays ◽  
Cell Adherence ◽  
Cancerous Cell ◽  
Primary Colon ◽  
Radiation Induced

This study examined the effects of gold nanoparticles (AuNPs) and/or ionizing radiation (IR) on the viability and motility of human primary colon epithelial (CCD841) and colorectal adenocarcinoma (SW48) cells as well as human primary epidermal melanocytes (HEM) and melanoma (MM418-C1) cells. AuNPs up to 4 mM had no effect on the viability of these cell lines. The viability of the cancer cells was ~60% following exposure to 5 Gy. Exposure to 5 Gy X-rays or 1 mM AuNPs showed the migration of the cancer cells ~85% that of untreated controls, while co-treatment with AuNPs and IR decreased migration to ~60%. In the non-cancerous cell lines gap closure was enhanced by ~15% following 1 mM AuNPs or 5 Gy treatment, while for co-treatment it was ~22% greater than that for the untreated controls. AuNPs had no effect on cell re-adhesion, while IR enhanced only the re-adhesion of the cancer cell lines but not their non-cancerous counterparts. The addition of AuNPs did not enhance cell adherence. This different reaction to AuNPs and IR in the cancer and normal cells can be attributed to radiation-induced adhesiveness and metabolic differences between tumour cells and their non-cancerous counterparts.

scholarly journals Data describing the effects of potassium channels modulators on outward currents measured in human lymphoma cell lines

Data in Brief ◽  
2021 ◽  
Vol 34 ◽  
pp. 106668
Author(s):  
Alberto Montalbano ◽  
Cesare Sala ◽  
Chiara Abrardo ◽  
Nicoletta Murciano ◽  
Farhad Jahanfar ◽  
...  
Keyword(s):  
Potassium Channels ◽  
Cell Lines ◽  
Lymphoma Cell ◽  
Outward Currents ◽  
Human Lymphoma

Paclitaxel restores radiation-induced apoptosis in a bcl-2-expressing, radiation-resistant lymphoma cell line

2001 ◽  
Vol 49 (4) ◽  
pp. 1127-1132 ◽  
Author(s):  
Linh N Nguyen ◽  
Anupama Munshi ◽  
Marvette L Hobbs ◽  
Mike D Story ◽  
Raymond D Meyn
Keyword(s):  
Cell Line ◽  
Lymphoma Cell ◽  
Lymphoma Cell Line ◽  
Radiation Resistant ◽  
Radiation Induced ◽  
Induced Apoptosis

Granulocyte-Macrophage Colony-Stimulating Factor Rescues TF-1 Leukemia Cells From Ionizing Radiation-Induced Apoptosis Through a Pathway Mediated by Protein Kinase Cα

Blood ◽  
1998 ◽  
Vol 92 (2) ◽  
pp. 416-424 ◽  
Author(s):  
Mary L. Kelly ◽  
Yan Tang ◽  
Nitsa Rosensweig ◽  
Sanda Clejan ◽  
Barbara S. Beckman
Keyword(s):  
Protein Kinase ◽  
Ionizing Radiation ◽  
Colony Stimulating Factor ◽  
Gm Csf ◽  
Pkc Isoforms ◽  
Radiation Induced ◽  
Macrophage Colony Stimulating Factor ◽  
Macrophage Colony ◽  
Induced Apoptosis ◽  
Stimulating Factor

Abstract Protein kinase C (PKC) activity has a recognized role in mediating apoptosis. However, the role of individual PKC isoforms in apoptosis is poorly defined. Therefore, we investigated the translocation of individual PKC isoforms during radiation-induced apoptosis with and without rescue from apoptosis by granulocyte-macrophage colony-stimulating factor (GM-CSF) in the human erythroleukemia cell line TF-1. PKCα was translocated from the particulate to cytosolic fraction of TF-1 cells within 5 minutes of treatment with apoptosis-inducing levels of ionizing radiation. However, this postirradiation translocation did not occur when cells were rescued from apoptosis by GM-CSF. Furthermore, treatment of cells with Gö6976, an inhibitor of classical PKC isoforms, abrogated the rescue effect of GM-CSF. The calcium-independent novel PKC isoform, PKCδ appeared to be degraded in both the particulate and cytosolic fractions of TF-1 cells after treatment with apoptosis-inducing levels of ionizing radiation in either the presence or absence of GM-CSF rescue. Levels of ceramide, a lipid mediator of apoptosis, were measured at 2, 4, 8, 10, and 60 minutes after treatment with ionizing radiation and were substantially reduced in TF-1 cells rescued from apoptosis by GM-CSF compared with apoptotic TF-1 cells. The largest decrease in ceramide production seen was at 4 minutes postirradiation, with a 46% reduction in ceramide levels in TF-1 cells rescued from apoptosis by GM-CSF compared with those in apoptotic TF-1 cells. Because ceramide has been shown to affect PKCα subcellular distribution, these data implicate a role for ceramide in mediating the rapid postirradiation translocation and inhibition of PKCα in TF-1 cells not rescued from apoptosis by GM-CSF. Expression of the antiapoptotic protein Bcl-2 doubled in TF-1 cells rescued from apoptosis by GM-CSF, but did not increase in unrescued cells. Our findings suggest that activated PKCα and increased expression of Bcl-2 after γ irradiation determine survival in TF-1 cells rescued from apoptosis with GM-CSF and that PKCδ plays a role in mediating signals involved in sensing cellular damage and/or regulation of cell damage repair.

scholarly journals The Inhibition of γ-Radiation Induced Apoptosis in Astrocytoma-Derived Cell Lines Is Associated with a Failure of Ceramide Synthesis and Fas-Mediated Apoptosis

1999 ◽  
Vol 45 (4, Part 2 of 2) ◽  
pp. 148A-148A
Author(s):  
Hiroko Kuriyama ◽  
Garret L Yount ◽  
Nagato Kuriyama ◽  
Mark A Israel
Keyword(s):  
Cell Lines ◽  
Γ Radiation ◽  
Ceramide Synthesis ◽  
Radiation Induced ◽  
Induced Apoptosis
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