High expression of B23 is associated with tumorigenesis and poor prognosis in bladder urothelial carcinoma

Background. Neurovascular-related genes have been implicated in the development of cancer. Studies have shown that a high expression of neuropilins (NRPs) promotes tumourigenesis and tumour malignancy. Method. A multidimensional bioinformatics analysis was performed to examine the relationship between NRP genes and prognostic and pathological features, tumour mutational burden (TMB), microsatellite instability (MSI), and immunological features based on public databases and find the potential prognostic value of NRPs in pancancer. Results. Survival analysis revealed that a low NRP1 expression in adrenocortical carcinoma (ACC), cervical squamous cell carcinoma and endocervical adenocarcinoma (CESC), low-grade glioma (LGG), and stomach adenocarcinoma (STAD) was associated with poor prognosis. A high NRP2 expression in bladder urothelial carcinoma (BLCA), kidney renal papillary cell carcinoma (KIRP), and mesothelioma (MESO) was associated with poor prognosis. Moreover, NRP1 and NRP2 were associated with TMB and MSI. Subsequent analyses showed that NRP1 and NRP2 were correlated with immune infiltration and immune checkpoints. Genome-wide association analysis revealed that the NRP1 expression was strongly associated with kidney renal clear cell carcinoma (KIRC), whereas the NRP2 expression was closely associated with BLCA. Ultimately, NRP2 was found to be involved in the development of BLCA. Conclusions. Neurovascular-related NRP family genes are significantly correlated with cancer prognosis, TME, and immune infiltration, particularly in BLCA.

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233 Glucose transporter-3 expression predicts poor prognosis in human bladder urothelial carcinoma and is associated with epithelial mesenchymal transition

European Urology Supplements ◽

10.1016/s1569-9056(15)60232-9 ◽

2015 ◽

Vol 14 (2) ◽

pp. e233

Author(s):

Y-C. Ou ◽

Y-S. Tsai ◽

C-J. Liu ◽

Y-L. Kao ◽

K-Y. Wu ◽

...

Keyword(s):

Urothelial Carcinoma ◽

Poor Prognosis ◽

Glucose Transporter ◽

Epithelial Mesenchymal Transition ◽

Human Bladder ◽

Mesenchymal Transition ◽

Bladder Urothelial Carcinoma

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Expression of transcription factor SALL4 in bladder urothelial carcinoma and its relationship with epithelial mesenchymal transformation

10.21203/rs.3.rs-1195553/v1 ◽

2021 ◽

Author(s):

Wenmin Zhang

Keyword(s):

Bladder Cancer ◽

Transcription Factor ◽

Protein Expression ◽

Urothelial Carcinoma ◽

Cancer Cells ◽

Poor Prognosis ◽

Migration And Invasion ◽

Bladder Urothelial Carcinoma ◽

Bladder Cancer Cells ◽

Mesenchymal Transformation

Abstract Purpose To evaluate the expression levels of spalt-like transcription factor 4 (SALL4) in bladder urothelial carcinoma, and determine its role and underlying mechanism of action in mediating the proliferation, migration and invasion of bladder cancer cells. Methods SALL4 expression was examined in 170 bladder patient urothelial carcinoma tissue samples by immunohistochemistry. Using a SALL4 overexpression plasmid and siRNA-SALL4, the effects of overexpressing and silencing SALL4 on the proliferation, migration and invasion of T24 and 5637 bladder cancer cells was examined using CCK8, migration and invasion assays. Western blot analysis was performed to detect epithelial mesenchymal transition (EMT)-related protein expression. Results The expression rate of SALL4 in low-grade and high-grade urothelial carcinomas was found to be 10% and 49.12%, respectively (P < 0.001), while SALL4 expression was not observed in the normal urothelium. SALL4 protein expression was positively correlated with histological grade, depth of invasion, lymphatic metastasis and vascular invasion of bladder urothelial carcinoma (P < 0.05). In addition, a shorter overall survival time and poor prognosis was observed in the SALL4 protein expression group. Overexpression of SALL4 led to significantly increased cell proliferation, migration and invasion, while knockdown of SALL4 had the opposite effect. In the SALL4 overexpression group, N-cadherin, vimentin, Snail and β-catenin expression were significantly increased, while E-cadherin expression was significantly decreased (P < 0.05). Promotion of EMT was also observed in SALL4-overexpressing cells. In contrast, in the SALL4-siRNA-treated group, EMT was reversed and β-catenin expression was reduced. Conclusions Our data show that the SALL4 gene is associated with the proliferation, invasion and poor prognosis of bladder urothelial carcinoma, and may mediate its effects via the Wnt/β-catenin signaling pathway, which regulates the EMT pathway. Thus, down-regulation of SALL4 may provide a novel therapeutic strategy for the treatment of bladder urothelial carcinoma.

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