Expression of TRIP13, ZEB1, and E-cad in Infiltrating Urothelial Carcinoma of Bladder and Their Clinical Significance

Background and purpose: Thyroid hormone receptor interacting protein 13 (TRIP13) which plays a carcinogenic role in tumors belongs to AAA+ ATPase superfamily. Zinc finger E-box binding homeobox 1 (ZEB1) and epithelial cadherin (E-cad), the key biomarkers of epithelial-mesenchymal transition (EMT), which aberrant expression may promote tumor invasion and metastasis. The purpose of this study is to investigate the expression of TRIP13, ZEB1, and E-cad in infiltrating urothelial carcinoma of bladder (IUCB) and their clinicopathological significance.Methods: The expression of TRIP13, ZEB1, and E-cad in 150 cases of IUCB samples were investigated by immunohistochemistry. Clinicopathological, demography, and follow-up data were also collected.Results: Positive rates of expression of TRIP13 and ZEB1 were significantly higher in IUCB samples when compared with the control bladder samples. And positive rate of expression of E-cad was significantly lower in IUCB than its expression rate in the control group. The positive expression of TRIP13 and ZEB1 were positively associated with tumor stages, local lymph node metastasis (LNM), and tumor node metastasis (TNM) stages. And E-cad expression was negatively associated with tumor stages, LNM, and TNM stages. The results of Kaplan-Meier analysis demonstrated that the patients with TRIP13 and ZEB1 positive expression had an unfavorable overall survival (OS) time when compared with patients with TRIP13 and ZEB1 negative expression. And patients with E-cad positive expression had a favorable OS time when compared with patients with E-cad negative expression. The COX regression analysis indicated that TRIP13, ZEB1, and E-cad, tumor stages, as well as TNM stages were independently factors for OS time in IUCB patients with postoperative therapy.Conclusions: TRIP13, ZEB1, and E-cad may be considered as potentially promising biomarkers for IUCB patients’ prognosis.

The Association of Clinicopathological Features with Metastatic Status in Infiltrative Urothelial Carcinoma of Bladder

Background: Infiltrative urothelial carcinoma of bladder is an aggressive variant of bladder carcinoma. Most of the patients in their first encounter with doctors are diagnosed with advanced stages and many has metastasized. Metastasis is a process of malignant tumor cells migrating from its primary tumor location to other organs or sites inside the body via blood circulation or lymphatic system. Metastasis process is responsible for 90% cancer deaths compared to the primary tumor itself, infiltrative urothelial carcinoma of bladder. To reduce mortality, knowledge of the clinicopathological characteristics associated with metastasis is needed. Method: Research material used was clinical data and paraffin block from patients with metastatic and non-metastatic infiltrative urothelial carcinoma of bladder. Clinical data taken from Central Medical Record Installation and Urology Medical Record Installation Padjadjaran University/ Dr. Hasan Sadikin Hospital Bandung, Indonesia. Paraffin blocks were obtained from operations at Hasan Sadikin Hospital Bandung, Indonesia.Total data sample obtained were 40 samples, 20 samples are metastatic and another 20 are non-metastatic samples. Clinicopathological parameters included in the analysis were age, sex and pathalogical T stage. Result: Pathalogical T stage have associations with metastatic status with significant result with p value=0.035 (p<0.005). Conclusion: Pathalogical T stage was associated with the metastatic status of Infiltrative urothelial carcinoma of bladder and may play important roles during the metastatic process. Thus, it can be used as a predictor factor for the infiltrative urothelial carcinoma of bladder. Keywords: infiltrative urothelial carcinoma of bladder, metastastic, pathalogical T stage