Classification of neural differentiation-associated genes in P19 embryonal carcinoma cells by their expression patterns induced after cell aggregation and/or retinoic acid treatment

2005 ◽  
Author(s):  
Sayaka Teramoto ◽  
Fumiko Kihara-Negishi ◽  
Takuya Sakurai ◽  
Toshiyuki Yamada ◽  
Tomoko Hashimoto-Tamaoki ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Acid Treatment ◽  
Embryonal Carcinoma ◽  
Expression Patterns ◽  
Cell Aggregation ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Retinoic Acid Treatment ◽  
P19 Embryonal Carcinoma Cells

scholarly journals Genome-wide demethylation during neural differentiation of P19 embryonal carcinoma cells

2008 ◽  
Vol 53 (2) ◽  
pp. 185-191 ◽  
Author(s):  
Izuho Hatada ◽  
Sumiyo Morita ◽  
Mika Kimura ◽  
Takuro Horii ◽  
Riu Yamashita ◽  
...  
Keyword(s):  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Genome Wide ◽  
P19 Embryonal Carcinoma Cells

scholarly journals Activation of c-Jun amino-terminal kinase is required for retinoic acid-induced neural differentiation of P19 embryonal carcinoma cells

FEBS Letters ◽  
2001 ◽  
Vol 503 (1) ◽  
pp. 91-96 ◽  
Author(s):  
Hong Wang ◽  
Shoko Ikeda ◽  
Shin-ichiro Kanno ◽  
Li Ming Guang ◽  
Motoko Ohnishi ◽  
...  
Keyword(s):  
Retinoic Acid ◽  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
Amino Terminal ◽  
P19 Embryonal Carcinoma Cells

scholarly journals Okadaic Acid Suppresses Neural Differentiation-dependent Expression of the Neurofilament-L Gene in P19 Embryonal Carcinoma Cells by Post-transcriptional Modification

1996 ◽  
Vol 271 (42) ◽  
pp. 25950-25957 ◽  
Author(s):  
Yoji Sasahara ◽  
Takayasu Kobayashi ◽  
Hiroshi Onodera ◽  
Masatoshi Onoda ◽  
Motoko Ohnishi ◽  
...  
Keyword(s):  
Okadaic Acid ◽  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Carcinoma Cells ◽  
Embryonal Carcinoma Cells ◽  
P19 Embryonal Carcinoma Cells

Efficiency of Neural Differentiation of Mouse P19 Embryonal Carcinoma Cells is Dependent on the Seeding Density

1997 ◽  
Vol 6 (5) ◽  
pp. 521-525 ◽  
Author(s):  
Hiroshi Kitani ◽  
Hidetoshi Ikeda ◽  
Tadao Atsumi ◽  
Rihito Watanabe
Keyword(s):  
Gene Transfer ◽  
Neural Differentiation ◽  
Embryonal Carcinoma ◽  
Ex Vivo ◽  
Carcinoma Cells ◽  
Vector System ◽  
Seeding Density ◽  
P19 Cells ◽  
Embryonal Carcinoma Cells ◽  
P19 Embryonal Carcinoma Cells

Serum-free culture conditions for retinoic acid-induced neural differentiation of mouse P19 embryonal carcinoma cells were determined for future ex vivo retroviral gene transfer and brain transplantation studies. Neural differentiation of P19 cells was dependent on the seeding densities, and both neurons and astroglia differentiated efficiently at high seeding densities (2 × 104 and 5 × 104 cells/cm2) but not at low seeding density (1 × 104 cells/cm2). In addition, P19 cells cultured at 5 × 104 cells/cm2 showed neural differentiation whether or not they were infected with Friend leukemia virus FrC6-V, which inhibited neural differentiation at 2 × 104 cells/cm2. These results indicate that FrC6-V-infected P19 embryonal carcinoma cells should be seeded at high density to achieve efficient neural differentiation in vitro for ex vivo gene transfer with a FrC6-V–derived retroviral vector system.

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