Timing of apoptosis onset depends on cell cycle progression in peripheral blood lymphocytes and lymphocytic leukemia cells

The aim of this work is to determine the duration of goat cell cycling in vitro.Goat peripheral blood lymphocytes were grown in RPMI-1640 medium containing bromodeoxyuridine (BrdU 10 μg/ml) for 72 h. Blastogenic index (BI), mitotic index (MI), cell cycle progression (CCP) and sister chromatid exchanges (SCE) were determined. Cultured lymphocytes from, whole blood or from leukocyte rich plasma in RPMI-1640 medium containing BrdU showed little differences in BI, MI, but significant differences were seen in cell cycle progression. BI, MI, and CCP from different goat breed were compared. Also, the percentage of lymphocyte blastogenesis, mitoses and cell cycle progression from goat, were compared to those from sheep, and human whichgrown under similar conditions. On successive incubation periods, the cell cycle duration of blood lymphocytes was determined through the mitotic activity. The cells reached first, second and third mitoses after 25, 40 and 48 h, post incubation respectively. Sub culturing of growing lymphocytes was performed from 3 to 45 days to obtain a lymphoblastoid cells. The characterization of their differentiation is required Establishment of goat blood lymphocyte culture will help in gene marker’s detection in their somatic cells.

Download Full-text

Metformin inhibits cell cycle progression of B-cell chronic lymphocytic leukemia cells

Oncotarget ◽

10.18632/oncotarget.4168 ◽

2015 ◽

Vol 6 (26) ◽

pp. 22624-22640 ◽

Cited By ~ 16

Author(s):

Silvia Bruno ◽

Bernardetta Ledda ◽

Claudya Tenca ◽

Silvia Ravera ◽

Anna Maria Orengo ◽

...

Keyword(s):

Cell Cycle ◽

Chronic Lymphocytic Leukemia ◽

B Cell ◽

Cell Cycle Progression ◽

Lymphocytic Leukemia ◽

Leukemia Cells ◽

Cycle Progression ◽

Cell Chronic Lymphocytic Leukemia

Download Full-text

Applications of Human Peripheral Blood Lymphocytes in Genotoxicity and Cytotoxicity

Alternatives to Laboratory Animals ◽

10.1177/026119299001800123.1 ◽

1990 ◽

Vol 18 (1_part_1) ◽

pp. 231-241

Author(s):

Lucia Celotti ◽

Vera Bianchi

Keyword(s):

Cell Cycle ◽

Peripheral Blood ◽

Mammalian Cells ◽

Cell Cycle Progression ◽

Human Peripheral Blood ◽

Quantitative Difference ◽

Peripheral Blood Lymphocytes ◽

Blood Lymphocytes

A number of features make peripheral blood lymphocytes an excellent system for studying both genotoxicity and cytotoxicity in humans. They are an abundant and readily accessible source of somatic cells, mostly in a non-proliferative state, but able to be stimulated by mitogens to enter the cell cycle. The blastocyte transformation of lymphocytes is a useful model for investigating the mechanisms which regulate cell-cycle progression in mammalian cells. By stimulating lymphocytes in vitro, it is possible to detect the genetic damages they have sustained in vivo, which become manifest as chromosomal aberrations, sister-chromatid exchanges or gene mutations. The metabolic properties of lymphocytes have been extensively studied, especially with reference to their characteristic collection of enzymes involved in nucleotide turnover, which makes them exquisitely sensitive to changes in intracellular levels of DNA precursors. The data collected on the ability of lymphocytes to metabolise xenobiotics show a marked quantitative difference between resting and proliferating lymphocytes, and minor qualitative differences between lymphocytes and other cell types, e.g. hepatocytes. An indirect approach to detect the metabolism of genotoxic xenobiotics by lymphocytes is the analysis of DNA adducts in their chromatin after in vivo or in vitro exposure. Lymphocytes can be employed to identify the (cyto)genetic consequences of in vivo genotoxic exposure and inter-individual variation in sensitivity to genotoxic agents. The analysis of mutations at the hgprt locus in lymphocytes is a promising approach for the study of somatic-cell mutations in humans and of the possible mechanisms of in vivo selection against mutants. In the field of cytotoxicity, the applications of lymphocytes are, as yet, still few: the main effect measured is the impairment of the proliferative response to mitogens. But lymphocytes can be employed as primary human cells to be treated in vitro with mutagenic or toxic chemicals in standard genotoxicity and cytotoxicity assays, and offer the advantage of avoiding the problems of inter-species extrapolation of results by testing in a human system. Moreover, the (geno)toxic effects detected in lymphocytes after treatments in vitro may give information on the spontaneous or environmentally-determined susceptibility of the individual donors to xenobiotics.

Download Full-text

Cell cycle progression of chronic lymphocytic leukemia cells is controlled by cyclin D2, cyclin D3, cyclin-dependent kinase (cdk) 4 and the cdk inhibitor p27

Leukemia ◽

10.1038/sj.leu.2402389 ◽

2002 ◽

Vol 16 (3) ◽

pp. 327-334 ◽

Cited By ~ 68

Author(s):

T Decker ◽

F Schneller ◽

S Hipp ◽

C Miething ◽

T Jahn ◽

...

Keyword(s):

Cell Cycle ◽

Chronic Lymphocytic Leukemia ◽

Cell Cycle Progression ◽

Lymphocytic Leukemia ◽

Cyclin D3 ◽

Leukemia Cells ◽

Cdk Inhibitor ◽

Cyclin Dependent Kinase ◽

Cyclin D2 ◽

Cycle Progression

Download Full-text

Visualization of tubulin in lymphocytes. I. Comparison of normal and chronic lymphocytic leukemia (CLL) lymphocytes

Blood ◽

10.1182/blood.v51.6.1031.1031 ◽

1978 ◽

Vol 51 (6) ◽

pp. 1031-1037 ◽

Cited By ~ 4

Author(s):

G Dighiero ◽

E Karsenti ◽

JY Follezou ◽

M Bornens

Keyword(s):

Chronic Lymphocytic Leukemia ◽

Peripheral Blood ◽

Normal Subjects ◽

Peripheral Blood Lymphocytes ◽

Lower Number ◽

Lymphocytic Leukemia ◽

Blood Lymphocytes ◽

Cell Structures ◽

Immunoperoxidase Assay

Abstract Cell structures containing tubulin were studied in peripheral blood lymphocytes from 8 normal donors and 11 patients with CLL using specific antitubulin antibodies revealed by immunoperoxidase assay. The centriole and microtubules were clearly visible in both groups. A “nucleus-associated tubulin-containing structure” was revealed by antitubulin antibodies and was found in virtually all lymphocytes of normal subjects but in a considerably lower number of CLL lymphocytes. The nature of this structure and its relationship to other cell structures are discussed.

Download Full-text