scholarly journals Latent membrane protein 1 mediates the resistance of nasopharyngeal carcinoma cells to TRAIL-induced apoptosis by activation of the PI3K/Akt signaling pathway

2011 ◽  
Author(s):  
Xin-Ming Yang
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Membrane Protein ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Latent Membrane Protein ◽  
Carcinoma Cells ◽  
Latent Membrane Protein 1 ◽  
Akt Signaling Pathway ◽  
Induced Apoptosis

Latent Membrane Protein 1 Antibody Inhibited Cell Stemness Growth of Nasopharyngeal Carcinoma by Regulating PI3K/AKT Signaling Pathway

2020 ◽  
Vol 10 (6) ◽  
pp. 768-775
Author(s):  
Dawei Zhang ◽  
Lin Xiong ◽  
Liang Li ◽  
Yuan Chen ◽  
Xiaojun Tang ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Signaling Pathway ◽  
Akt Signaling ◽  
Latent Membrane Protein 1 ◽  
The Novel ◽  
Akt Signaling Pathway ◽  
Akt Phosphorylation ◽  
Sphere Formation

Objective: The aim of the study was to investigate the effects of LMP1-Fab antibody on Nasopharyngeal carcinoma (NPC) cancer stem cells (CSCs). Methods/Results: LMP1 was identified to play an important role in maintaining the stemness characteristics of NPC by sphere formation, as is evidenced by CCK-8 and colony formation assays. Furthermore, CCK8 and TUNEL assays indicated that LMP1-Fab antibody could accelerate cell apoptosis while inhibiting cell proliferation in nasopharyngeal carcinoma stem cell. Notably, LMP1-Fab antibody inhibited NPC xenograft cancer growth in the nude mice. At the molecular level, LMP1-Fab antibody inhibited the phosphorylation of PI3K/AKT signaling pathway both in vitro and in vivo. More importantly, these effects were blocked by PI3K/AKT phosphorylation inhibitor LY294002. Conclusion: These results suggested that the novel antibody-targeting LMP1 is likely to be a promising strategy for the treatment of NPC.

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