Blood culture at 63 Japanese healthcare facilities

Background: Malaria and bacteremia co-morbidity in children cause changes in blood cellular components. Complete blood count from children whose haemoglobin genotypes and bacteremia tests are not known, greatly influence clinical management and interpretation of the haematology results in resource limited healthcare facilities. Objectives: We investigated cellular components from children with bacteremia and malaria co-morbidity. We also analysed the haemoglobin genotypes and bacteria isolates from children with haemoglobin AA, SS and AS in western Kenya. Methods: A total number of 384 children were recruited and complete blood counts done with an automated cell counter. Microscopy was used to determine malaria infections, while bacteremia was determined by blood culture. The haemoglobin genotypes were analysed using the electrophoresis technique. Results: Children with haemoglobin AA and AS had elevated granulocyte counts. Most of the bacteria isolates were from children with malaria and haemoglobin AS. The bacteria isolated from blood culture included non-typhi salmonella, Escherichia coli, Enterobacter cloacae, Staphylococcus aureus, Listeria monocytogenes, Streptococcus pyogenes and Viridans. Salmonella species and staphylococcus aureus were the most prevalent bacteria isolates associated with bacteremia in children with haemoglobin AS and malaria positive. Conclusion: Children with Hb AS have higher chances of malaria and bacterial co-infection which leads to lymphocytopenia, erythrocytopenia and thrombocytopenia. Bacteria responsible for most of malaria co-infections in this region are Salmonella species and Staphylococcus aureus. The malaria and bacterial co-infection in pre-school children initiate differential cellular changes which should be investigated further.

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ANTIMICROBIAL SUSCEPTIBILITY IN CULTURE POSITIVE ENTERIC FEVER

International Journal of Medical and Biomedical Studies ◽

10.32553/ijmbs.v3i7.357 ◽

2019 ◽

Vol 3 (7) ◽

Author(s):

Dr. Manish Kulshrestha ◽

Dr. Anjali Kulshrestha

Keyword(s):

Bone Marrow ◽

Blood Culture ◽

Nalidixic Acid ◽

Antimicrobial Susceptibility ◽

Salmonella Enterica ◽

Enteric Fever ◽

Multidrug Resistant ◽

Bone Marrow Culture ◽

Diffusion Method ◽

Culture Positive

INTRODUCTION: Enteric fever includes typhoid and paratyphoid fever. Peak incidence is seen in children 5–15 years of age; but in regions where the disease is highly endemic, as in India, children younger than 5 years of age may have the highest infection rates. There are about 22 million new typhoid cases occur each year. Young children in poor, resource limited areas, who make up the majority of the new cases and there is a mortality figures of 215,000 deaths annually. A sharp decline in the rates of complications and mortality due to typhoid fever is observed as a result of introduction of effective antibiotic therapy since 1950s. MDR-ST became endemic in many areas of Asia, including India soon after multidrug-resistant strains of Salmonella enterica serotype typhi (MDR-ST) that were resistant to all the three first-line drugs then in use, namely chloramphenicol, amoxycillin and co-trimoxazole emerged in early 1990s. MATERIAL AND METHODS: Only blood culture or bone marrow culture positive cases were included. The patients with culture isolated enteric fever were included in the study. Antimicrobial susceptibility testing was carried out by disk diffusion method using antibiotic discs. The analysis of the antimicrobial susceptibility was carried out as per CLSI interpretative guidelines. RESULTS: A total of 82 culture positive cases were included in the present study. 80 culture isolates were from blood culture and 2 from the bone marrow culture. Salmonella entericasubspecies enterica serovartyphi (S typhi) was isolated from 67 (81.70%) patients while Salmonella enterica subspecies entericaserovarparatyphi (S paratyphi A) was isolated from 13 (15.85%) cases and 2 (2.44%) were Salmonella enterica subspecies entericaserovarschottmuelleri (S paratyphi B). Of the 82 cases 65(79.3%) isolates were resistant to ciprofloxacin, 17 (20.7%) were resistant to nalidixic acid, one (1.2%) case each was resistant to Cefotaxime and ceftriaxone, 2 (2.4%) were resistant to chloramphenicol, 10 (12.2%) were resistant and to cotrimoxazole 3 (3.7%) were resistant. CONCLUSION: In a culture positive cases 65(79.3%) isolates were resistant to ciprofloxacin and 17 (20.7%) were resistant to nalidixic acid. Multidrug resistant isolates were 65(79.3%).

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Inefficiency in 6-Set Requests for Blood Culture Analysis of Cancer Patients with the Central Venous Catheter

Laboratory Medicine Online ◽

10.3343/lmo.2013.3.3.155 ◽

2013 ◽

Vol 3 (3) ◽

pp. 155

Author(s):

Dong-Hyun Lee ◽

Eun-ha Koh ◽

Sunjoo Kim ◽

In-Gyu Bae ◽

Hoon-gu Kim ◽

...

Keyword(s):

Blood Culture ◽

Central Venous Catheter ◽

Cancer Patients ◽

Venous Catheter ◽

Central Venous

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Duration of Antibiotic Therapy in VLBW Infants with Exposure to Chorioamnionitis and Negative Blood Culture: Their Outcomes

10.1542/peds.140.1_meetingabstract.67 ◽

2017 ◽

Author(s):

Chibuzor Nonye Opara ◽

John H Stroger

Keyword(s):

Blood Culture ◽

Antibiotic Therapy ◽

Negative Blood Culture ◽

Vlbw Infants

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Duration of Antibiotic Therapy in VLBW Infants with Exposure to Chorioamnionitis and Negative Blood Culture: Their Outcomes:

PEDIATRICS ◽

10.1542/peds.137.supplement_3.469a ◽

2016 ◽

Vol 137 (Supplement 3) ◽

pp. 469A-469A

Author(s):

Chibuzor Nonye Opara ◽

John H Stroger

Keyword(s):

Blood Culture ◽

Antibiotic Therapy ◽

Negative Blood Culture ◽

Vlbw Infants

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RESEARCH: Dry Heat Processing of Single-Use Respirators and Surgical Masks

Biomedical Instrumentation & Technology ◽

10.2345/0899-8205-54.6.410 ◽

2020 ◽

Vol 54 (6) ◽

pp. 410-416

Author(s):

Joyce M. Hansen ◽

Scott Weiss ◽

Terra A. Kremer ◽

Myrelis Aguilar ◽

Gerald McDonnell

Keyword(s):

Microbial Contamination ◽

Healthcare Providers ◽

High Volume ◽

Airborne Particles ◽

Heat Processing ◽

Protective Equipment ◽

Healthcare Facilities ◽

Dry Heat ◽

Surgical Masks ◽

Single Use

The COVID-19 pandemic, caused by severe acute respiratory syndrome coronavirus 2, has challenged healthcare providers in maintaining the supply of critical personal protective equipment, including single-use respirators and surgical masks. Single-use respirators and surgical masks can reduce risks from the inhalation of airborne particles and microbial contamination. The recent high-volume demand for single-use respirators and surgical masks has resulted in many healthcare facilities considering processing to address critical shortages. The dry heat process of 80°C (176°F) for two hours (120 min) has been confirmed to be an appropriate method for single-use respirator and surgical mask processing.

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