The presence of chronic bee paralysis virus infection in honey bees (Apis mellifera L.) in the USA

2011 ◽  
Vol 50 (1) ◽  
pp. 85-86 ◽  
Author(s):  
Yan Ping Chen ◽  
Jay D Evans ◽  
Jeff S Pettis
Keyword(s):  
Apis Mellifera ◽  
Virus Infection ◽  
Honey Bees ◽  
The Usa ◽  
Paralysis Virus

scholarly journals Discovery of Aphid Lethal Paralysis Virus in Vespa velutina and Apis cerana in China

Insects ◽  
2019 ◽  
Vol 10 (6) ◽  
pp. 157 ◽  
Author(s):  
Dahe Yang ◽  
Hongxia Zhao ◽  
Junming Shi ◽  
Xiang Xu ◽  
Yanyan Wu ◽  
...  
Keyword(s):  
Honey Bees ◽  
Apis Cerana ◽  
Terrestrial Ecosystems ◽  
Viral Reservoir ◽  
Molecular Evidence ◽  
Deformed Wing Virus ◽  
Vespa Velutina ◽  
Pollinator Abundance ◽  
The Usa ◽  
Paralysis Virus

Honey bees are essential to the functioning of terrestrial ecosystems. However, despite no single factor being blamed for losses of honey bee colonies in Europe and the USA, viruses have been considered as a major driver. Moreover, a virus vector can enhance the titer and virulence of virus such as Varroa destructor can change the virulence of the deformed wing virus. Here, we report molecular evidence for aphid lethal paralysis virus (ALPV) infecting Vespa velutina, which is an important predator of honey bees, especially of Apis cerana. Viral replication and phylogenetic analysis indicated that ALPV can not only replicate in V. velutina and A. cerana, but ALPV from A. cerana (ALPV-Ac) was also significantly associated with that of V. velutina (ALPV-Vv), though distinct from those of Apis mellifera (ALPV-Am). The host state posterior probability displayed that V. velutina is the main viral reservoir between V. velutina and A. cerana. Our results show ALPV had expanded host diversity resulting in potential impacts on the health of pollinators, even on the pollination ecosystem. We suggest further studies should investigate potential risks and impacts on pollinator populations of hornets. These results should have an impact conservation efforts focused on sustaining native pollinator abundance and diversity, and therefore, the crucial ecosystem services that they provide.

scholarly journals Heritability estimates of the novel trait ‘suppressed in ovo virus infection’ in honey bees (Apis mellifera)

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Dirk C. de Graaf ◽  
Dries Laget ◽  
Lina De Smet ◽  
David Claeys Boúúaert ◽  
Marleen Brunain ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Virus Infection ◽  
Honey Bees ◽  
The Novel ◽  
In Ovo ◽  
Heritability Estimates

scholarly journals A molecular clone of Chronic Bee Paralysis Virus (CBPV) causes mortality in honey bee pupae (Apis mellifera)

2019 ◽  
Vol 9 (1) ◽  
Author(s):  
Kerstin Seitz ◽  
Katharina Buczolich ◽  
Alžbeta Dikunová ◽  
Pavel Plevka ◽  
Karen Power ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Clinical Symptoms ◽  
Well Being ◽  
Reverse Genetic System ◽  
Progeny Virus ◽  
Molecular Clone ◽  
Depth Analysis ◽  
Paralysis Virus

Abstract Among the many diseases compromising the well-being of the honey bee (Apis mellifera) the chronic paralysis syndrome of adult honey bees is one of the best described. The causative agent, chronic bee paralysis virus (CBPV), is a positive sense, single-stranded RNA virus with a segmented genome. Segment 1 encodes three putative open reading frames (ORFs), including the RNA-dependent RNA polymerase and other non-structural protein coding regions. Segment 2 encodes four putative ORFs, which contain the genes of supposed structural proteins. In this study, we established a reverse genetic system for CBPV by molecular cloning of DNA copies of both genome segments. CBPV rescue was studied in imago and honey bee pupae infection models. Virus replication and progeny virus production was only initiated when capped RNAs of both genome segments were injected in honey bees. As injection of these clonal RNAs caused clinical symptoms similar to wild-type CBPV infection, we conclude that the novel molecular clone fulfilled Koch’s postulates. Our virus clone will enable in-depth analysis of CBPV pathogenesis and help to increase knowledge about this important honey bee disease.

scholarly journals Molecular Identification of Chronic Bee Paralysis Virus Infection in Apis mellifera Colonies in Japan

Viruses ◽  
2012 ◽  
Vol 4 (7) ◽  
pp. 1093-1103 ◽  
Author(s):  
Tomomi Morimoto ◽  
Yuriko Kojima ◽  
Mikio Yoshiyama ◽  
Kiyoshi Kimura ◽  
Bu Yang ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Virus Infection ◽  
Molecular Identification ◽  
Paralysis Virus

The paratransgenic potential of Lactobacillus kunkeei in the honey bee Apis mellifera

2015 ◽  
Vol 6 (4) ◽  
pp. 513-523 ◽  
Author(s):  
A. Rangberg ◽  
G. Mathiesen ◽  
G.V. Amdam ◽  
D.B. Diep
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Successful Outcome ◽  
Transformed Cells ◽  
Crop Failure ◽  
Effector Molecules ◽  
Model Animal ◽  
The Usa ◽  
Lactobacillus Kunkeei

The honey bee (Apis mellifera) is a domestic insect of high value to human societies, as a crop pollinator in agriculture and a model animal in scientific research. The honey bee, however, has experienced massive mortality worldwide due to the phenomenon Colony Collapse Disorder (CCD), resulting in alarming prospects for crop failure in Europe and the USA. The reasons for CCD are complex and much debated, but several honey bee pathogens are believed to be involved. Paratransgenesis is a Trojan horse strategy, where endogenous microorganisms are used to express effector molecules that antagonise pathogen development. For use in honey bees, paratransgenesis must rely on a set of criteria that the candidate paratransgenic microorganism must fulfil in order to obtain a successful outcome: (1) the candidate must be genetically modifiable to express effector molecules; (2) the modified organism should have no adverse effects on honey bee health upon reintroduction; and (3) it must survive together with other non-pathogenic bee-associated microorganisms. Lactic acid bacteria (LAB) are common gut bacteria in vertebrates and invertebrates, and some have naturally beneficial properties in their host. In the present work we aimed to find a potential paratransgenic candidate within this bacterial group for use in honey bees. Among isolated LAB associated with bee gut microbiota, we found the fructophilic Lactobacillus kunkeei to be the most predominant species during foraging seasons. Four genetically different strains of L. kunkeei were selected for further assessment. We demonstrated (1) that L. kunkeei is transformable; (2) that the transformed cells had no obvious adverse effect on honey bee survival; and (3) that transformed cells survived well in the gut environment of bees upon reintroduction. Our study demonstrates that L. kunkeei fulfils the three criteria for paratransgenesis and can be a suitable candidate for further research on this strategy in honey bees.

scholarly journals Egg transcriptome profile responds to maternal virus infection in honey bees, Apis mellifera

2020 ◽  
Vol 85 ◽  
pp. 104558
Author(s):  
Esmaeil Amiri ◽  
Jacob J. Herman ◽  
Micheline K. Strand ◽  
David R. Tarpy ◽  
Olav Rueppell
Keyword(s):  
Apis Mellifera ◽  
Virus Infection ◽  
Honey Bees ◽  
Transcriptome Profile

scholarly journals Resistance of native honey bees from Rhodope Mountains and lowland regions of Bulgaria to Nosema ceranae and viral pathogens

2020 ◽  
Vol 23 (2) ◽  
pp. 206-217
Author(s):  
R. Shumkova ◽  
B. Neov ◽  
A. Georgieva ◽  
D. Teofanova ◽  
G. Radoslavov ◽  
...  
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Viral Infections ◽  
Viral Pathogens ◽  
Deformed Wing Virus ◽  
Queen Cell ◽  
Kashmir Bee Virus ◽  
Paralysis Virus ◽  
Bee Viruses

The Western honey bee (Apis mellifera L., Hymenoptera: Apidae) is a species of fundamental economic, agricultural and environmental importance. The aim of this study was to compare the prevalence of some parasitic and viral pathogens in local honey bees from the Rodope Mountains and plain regions. To achieve this goal, molecular screening for two of the most distributed Nosema spp. and molecular identification of six honey bee viruses – Deformed wing virus (DWV), Acute bee paralysis virus (ABPV), Chronic bee paralysis virus (CBPV), Sacbrood virus (SBV), Kashmir bee virus (KBV), and Black queen cell virus (BQCV) was performed. Molecular analysis was carried out on 168 honey bee samples from apiaries situated in three different parts of the country where a mix of different honey bee subspecies were reared. In South Bulgaria (the Rhodope Mountains), a local honey bee called Apis mellifera rodopica (a local ecotype of A. m. macedonica) was bred, while in the other two regions (plains) different introduced subspecies existed. The results showed that the samples from the lowland regions in the country were outlined with the highest prevalence (70.5%) of N. ceranae, while those from the mountainous parts had the lowest rate (5.2%). Four of the honey bee viruses were identified – DWV (10/5.9%), followed by SBV (6/3.6%) and ABPV (2/1.2%), and one case of BQCV. In conclusion, the local honey bee A. m. rodopica (despite the higher number of samples) has shown lower prevalence of both nosemosis and viral infections. Therefore, this honey bee has to be preserved as a part of the national biodiversity.

scholarly journals Detection of Israeli Acute Paralysis Virus (IAPV) and Apis mellifera Filamentous Virus (AmFV) in Honey Bees in Mexico

2018 ◽  
Vol 62 (1) ◽  
pp. 141-144 ◽  
Author(s):  
Mayra C. García-Anaya ◽  
Alejandro Romo-Chacón ◽  
Alma I. Sáenz-Mendoza ◽  
Gerardo Pérez-Ordoñez ◽  
Carlos H. Acosta-Muñiz
Keyword(s):  
Apis Mellifera ◽  
Honey Bee ◽  
Honey Bees ◽  
Developmental Stages ◽  
Viral Diseases ◽  
Israeli Acute Paralysis Virus ◽  
Colony Loss ◽  
Polymerase Chain ◽  
Bee Colonies ◽  
Paralysis Virus

Abstract The recent alarming loss of honey bee colonies around the world is believed to be related to the presence of viruses. The aim of this study was to detect two major viral diseases, Apis mellifera Filamentous virus (AmFV) and Israeli Acute Paralysis Virus (IAPV) using Reverse Transcription - Polymerase Chain Reaction RT-PCR, in honey bees in Mexico. Adult and larvae honey bee samples were collected from asymptomatic colonies of six major beekeeping regions in the state of Chihuahua, Mexico. Both viruses were detected in both developmental stages of honey bees, IAPV at a higher prevalence (23.5%) as compared to AmFV, only in 0.9% of samples. However, this is the first report on AmFV infection in Mexican apiaries. Further studies are required to understand the AmFV and IAPV impact on colony loss in Mexico and to develop strategies for enhancing the control of viral diseases.

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