scholarly journals Three new species of soil-inhabiting Trichoderma from southwest China

MycoKeys ◽  
2018 ◽  
Vol 44 ◽  
pp. 63-80 ◽  
Author(s):  
Min Qiao ◽  
Xing Du ◽  
Zhe Zhang ◽  
JianPing Xu ◽  
ZenFen Yu
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Elongation Factor ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Closely Related Species ◽  
Encoding Gene ◽  
Three New Species ◽  
Elongation Factor 1 ◽  
Nuclear Rna Polymerase

Fungi in the genus Trichoderma are widely distributed in China, including in Yunnan province. In this study, we report three new soil-inhabiting species in Trichoderma, named as T.kunmingense, T.speciosum and T.zeloharzianum. Their colony and mycelial morphology, including features of asexual states, were described. For each species, their DNA sequences were obtained from three loci, the internal transcribed spacer (ITS) regions of the ribosomal DNA, the translation elongation factor 1-α encoding gene (tef1) and the gene encoding the second largest nuclear RNA polymerase subunit (rpb2). Our analyses indicated that the three new species showed consistent divergence amongst each other and from other known and closely related species. Amongst the three, T.speciosum and T.kunmingense belong to the Viride Clade. Specifically, T.speciosum is related to three species – T.hispanicum, T.samuelsii and T.junci and is characterised by tree-like conidiophores, generally paired branches, curved terminal branches, spindly to fusiform phialides and subglobose to globose conidia. In contrast, T.kunmingense morphologically resembles T.asperellum and T.yunnanense and is distinguished by its pyramidal conidiophores, ampulliform to tapered phialides, discrete branches and ovoidal, occasionally ellipsoid, smooth-walled conidia. The third new species, T.zeloharzianum, is a new member of the Harzianum Clade and is closely associated with T.harzianum, T.lixii and T.simmonsii but distinguished from them by having smaller, subglobose to globose, thin-walled conidia.

scholarly journals Four new species of Trichoderma in the Harzianum clade from northern China

MycoKeys ◽  
2020 ◽  
Vol 73 ◽  
pp. 109-132
Author(s):  
Xin Gu ◽  
Rui Wang ◽  
Quan Sun ◽  
Bing Wu ◽  
Jing-Zu Sun
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Elongation Factor ◽  
Northern China ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Nuclear Rna ◽  
Encoding Gene ◽  
Elongation Factor 1 ◽  
Nuclear Rna Polymerase

The Harzianum clade of Trichoderma comprises many species, which are associated with a wide variety of substrates. In this study, four new species of Trichoderma, namely T. lentinulae, T. vermifimicola, T. xixiacum, and T. zelobreve, were encountered from a fruiting body and compost of Lentinula, soil, and vermicompost. Their colony and mycelial morphology, including features of asexual states, were described. For each species, their DNA sequences were obtained from three loci, the internal transcribed spacer (ITS) regions of the ribosomal DNA, the gene encoding the second largest nuclear RNA polymerase subunit (RPB2), the translation elongation factor 1-α encoding gene (TEF1-α). The analysis combining sequences of the three gene regions distinguished four new species in the Harzianum clade of Trichoderma. Among them, T. lentinulae and T. xixiacum clustered with T. lixii, from which these new species differ in having shorter phialides and smaller conidia. Additionally, T. lentinulae differs from T. xixiacum in forming phialides with inequilateral to a strongly-curved apex, cultural characteristics, and slow growth on PDA. Trichoderma vermifimicola is closely related to T. simmonsii, but it differs from the latter by producing phialides in verticillate whorls and smaller conidia. Trichoderma zelobreve is the sister species of T. breve but is distinguished from T. breve by producing shorter and narrower phialides, smaller conidia, and by forming concentric zones on agar plates. This study updates our knowledge of species diversity of Trichoderma.

scholarly journals New Species of Trichoderma Isolated as Endophytes and Saprobes from Southwest China

2021 ◽  
Vol 7 (6) ◽  
pp. 467
Author(s):  
Hua Zheng ◽  
Min Qiao ◽  
Yifan Lv ◽  
Xing Du ◽  
Ke-Qin Zhang ◽  
...  
Keyword(s):  
New Species ◽  
Aquatic Plants ◽  
Southwest China ◽  
Elongation Factor ◽  
Gene Encoding ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Culture Characteristics ◽  
Close Relatives ◽  
Nuclear Rna Polymerase

During the investigation of endophytic fungi diversity in aquatic plants and the fungal diversity in soil in southwest China, we obtained 208 isolates belonging to Trichoderma, including 28 isolates as endophytes from aquatic plants and 180 isolates as saprobes from soil, respectively. Finally, 23 new species of Trichoderma are recognized by further studies. Their phylogenetic positions are determined by sequence analyses of the combined partial sequences of translation elongation factor 1-alpha (tef1) and gene encoding of the second largest nuclear RNA polymerase subunit (rpb2). The results revealed that the 23 new species are distributed in nine known clades. The morphology and culture characteristics are observed, described and illustrated in detail. Distinctions between the new species and their close relatives were compared and discussed. These include: Trichoderma achlamydosporum, T. amoenum, T. anaharzianum, T. anisohamatum, T. aquatica, T. asiaticum, T. asymmetricum, T. inaequilaterale, T. inconspicuum, T. insigne, T. obovatum, T. paraviride, T. pluripenicillatum, T. propepolypori, T. pseudoasiaticum, T. pseudoasperelloides, T. scorpioideum, T. simile, T. subazureum, T. subuliforme, T. supraverticillatum, T. tibetica, and T. uncinatum.

scholarly journals New species of Retiboletus (Boletales, Boletaceae) from China based on morphological and molecular data

MycoKeys ◽  
2020 ◽  
Vol 67 ◽  
pp. 33-44
Author(s):  
Hai-Ying Liu ◽  
Yan-Chun Li ◽  
Tolgor Bau
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Large Subunit ◽  
Elongation Factor ◽  
Molecular Data ◽  
Translation Elongation Factor ◽  
Translation Elongation ◽  
Line Drawings ◽  
Morphological And Molecular Data ◽  
Elongation Factor 1

Species of the genus Retiboletus in China were investigated based on morphology and phylogenetic analysis of DNA sequences from the nuclear ribosomal large subunit (nrLSU) and the translation elongation factor 1-α gene (TEF1-α). Nine species were recovered from China, including two new and seven known species. The new species, namely Retiboletus ater and R. sinogriseus, from southwestern and northeastern China respectively, are documented and illustrated in this paper. Retiboletus ater is morphologically characterized by its black to grayish black pileus, white to grayish hymenophore, black to blackish stipe and white to grayish white context. Retiboletus sinogriseus is morphologically characterized by its brown to grayish-brown pileus, yellow to grayish-yellow hymenophore, pale yellow to brownish stipe and yellow to brownish-yellow context. Descriptions and line drawings of these two novel species and their comparisons with allied taxa are presented.

Cantharellus macrocarpus (Cantharellaceae, Cantharellales), a new species from tropical China

Phytotaxa ◽  
2021 ◽  
Vol 484 (2) ◽  
pp. 170-180
Author(s):  
YU-ZHUO ZHANG ◽  
ZHI-QUN LIANG ◽  
HUI-JING XIE ◽  
LU-LING WU ◽  
ROU XUE ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Elongation Factor ◽  
Translation Elongation ◽  
Tropical Asia ◽  
Line Drawings ◽  
Yellow Orange ◽  
Tropical China ◽  
Elongation Factor 1 ◽  
A New Species

Cantharellus macrocarpus, a member of Cantharellus subgenus Cantharellus, is described as a new species from Hainan Province, China. It is characterized by large basidioma with a bright yellow-orange pileus and a well-developed, rugulose hymenophore, cream to pale yellowish stipe, noticeably thickened hyphal walls in the pileipellis, presence of clamp connections in all parts of the basidiomata, and a distribution in tropical Asia. A phylogenetic analysis of DNA sequences from part of the 28S gene and the translation elongation factor 1-a gene (TEF1) confirmed that it forms an independent lineage within subgenus Cantharellus. A detailed description, color photos of fresh basidiomata and line drawings of micromorphological features are presented.

Calonectria vigiensis sp. nov. (Hypocreales, Nectriaceae) associated with dieback and sudden-death symptoms of Theobroma cacao from Mérida state, Venezuela

Botany ◽  
2021 ◽  
pp. 683-693
Author(s):  
Sari R. Mohali ◽  
Jane E. Stewart
Keyword(s):  
New Species ◽  
Sudden Death ◽  
Theobroma Cacao ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Fungal Species ◽  
Translation Elongation ◽  
Closely Related Species ◽  
Theobroma Cacao L ◽  
Elongation Factor 1

Calonectria vigiensis sp. nov. (Hypocreales, Nectriaceae) is a newly described species associated with cacao (Theobroma cacao L.) in Mérida state, Venezuela. This new species was identified using morphological methods as well as DNA sequencing and multigene phylogenetic analyses. Based on phylogenetic analyses of a combined dataset including translation elongation factor 1-α (tef1), calmodulin (cmdA), and β-tubulin (btub) genes across closely related species within Calonectria, a new species was identified as Ca. vigiensis within the Calonectria naviculata species complex, but it was determined to be well-separated from genetically close species such as Calonectria multilateralis L. Lombard & Crous, Calonectria multinaviculata R.F. Alfenas, L. Lombard & Crous, and Calonectria naviculata Crous & M.J. Wingf. This work reports 1 previously undescribed fungal species (Ca. vigiensis) that is associated with dieback and sudden-death symptoms in T. cacao plantations in Mérida state, Venezuela.

scholarly journals A four-locus phylogeny of rib-stiped cupulate species of Helvella (Helvellaceae, Pezizales) with discovery of three new species

MycoKeys ◽  
2019 ◽  
Vol 60 ◽  
pp. 45-67
Author(s):  
Xin-Cun Wang ◽  
Tie-Zhi Liu ◽  
Shuang-Lin Chen ◽  
Yi Li ◽  
Wen-Ying Zhuang
Keyword(s):  
New Species ◽  
Large Subunit ◽  
Elongation Factor ◽  
Heat Shock Protein 90 ◽  
Sensu Stricto ◽  
Phylogenetic Study ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Three New Species ◽  
Elongation Factor 1

Helvella species are ascomycetous macrofungi with saddle-shaped or cupulate apothecia. They are distributed worldwide and play an important ecological role as ectomycorrhizal symbionts. A recent multi-locus phylogenetic study of the genus suggested that the cupulate group of Helvella was in need of comprehensive revision. In this study, all the specimens of cupulate Helvella sensu lato with ribbed stipes deposited in HMAS were examined morphologically and molecularly. A four-locus phylogeny was reconstructed using partial sequences of the heat shock protein 90, nuclear rDNA internal transcribed spacer region 2, nuclear large subunit ribosomal DNA and translation elongation factor 1-α genes. Three clades were revealed in Helvella sensu stricto. Twenty species were included in the analysis, of which 13 are distributed in China. Three new species, H. acetabuloides, H. sichuanensis and H. tianshanensis, are described and illustrated in detail. A neotype was designated for H. taiyuanensis. Helvella calycina is a new record for China, while Dissingia leucomelaena should be excluded from Chinese mycota. Hsp90 and ITS2 are recommended as useful supplementary barcodes for species identifications of the genus.

Amanita griseofusca: A new species of Amanita in section Vaginatae from Malam Jabba, Swat, Pakistan

Phytotaxa ◽  
2018 ◽  
Vol 364 (2) ◽  
pp. 181 ◽  
Author(s):  
MUNAZZA KIRAN ◽  
JUNAID KHAN ◽  
HASSAN SHER ◽  
DONALD H. PFISTER ◽  
ABDUL NASIR KHALID
Keyword(s):  
New Species ◽  
Elongation Factor ◽  
Molecular Data ◽  
Internal Transcribed Spacer Region ◽  
Translation Elongation ◽  
Elongation Factor 1 Alpha ◽  
Pileus Surface ◽  
Elongation Factor 1 ◽  
A New Species ◽  
Partial Translation

A new species, Amanita griseofusca in section Vaginatae is described and illustrated here from Pakistan. Distinguishing characters of the new species include medium-sized basidiomata, greyish brown pileus surface with white to beige, membranous volval remnants present as one (large) to a few (small) warts, close lamellae which are cream colored with a pink tone, striations one third of the total pileus radius, broadly ellipsoidal to ellipsoidal basidiospores and white loose saccate volva turning beige at maturity. Molecular data inferred from partial nuc rDNA internal transcribed spacer region (ITS), partial nuc rDNA larger subunit region (LSU) and partial translation elongation factor 1-alpha (tef1) confirms the novelty of the present taxon.

scholarly journals Survey, Identification, and Characterization of Cylindrocarpon-Like Asexual Morphs in Spanish Forest Nurseries

Plant Disease ◽  
2018 ◽  
Vol 102 (11) ◽  
pp. 2083-2100 ◽  
Author(s):  
Beatriz Mora-Sala ◽  
Ana Cabral ◽  
Maela León ◽  
Carlos Agustí-Brisach ◽  
Josep Armengol ◽  
...  
Keyword(s):  
Dna Sequences ◽  
Elongation Factor ◽  
Its Region ◽  
Phylogenetic Position ◽  
Translation Elongation ◽  
Forest Nurseries ◽  
Asexual Morphs ◽  
Identification And Characterization ◽  
Elongation Factor 1

Cylindrocarpon-like asexual morphs infect herbaceous and woody plants, mainly in agricultural scenarios, but also in forestry systems. The aim of the present study was to characterize a collection of Cylindrocarpon-like isolates recovered from the roots of a broad range of forest hosts from nurseries showing decline by morphological and molecular studies. Between 2009 and 2012, 17 forest nurseries in Spain were surveyed and a total of 103 Cylindrocarpon-like isolates were obtained. Isolates were identified based on DNA sequences of the partial gene regions histone H3 (his3). For the new species, the internal transcribed spacer and intervening 5.8S nrRNA gene (ITS) region, β-tubulin (tub2), and translation elongation factor 1-α (tef1) were also used to determine their phylogenetic position. Twelve species belonging to the genera Cylindrodendrum, Dactylonectria, and Ilyonectria were identified from damaged roots of 15 different host genera. The species C. alicantinum, D. macrodidyma, D. novozelandica, D. pauciseptata, D. pinicola, D. torresensis, I. capensis, I. cyclaminicola, I. liriodendri, I. pseudodestructans, I. robusta, and I. rufa were identified. In addition, two Dactylonectria species (D. hispanica sp. nov. and D. valentina sp. nov.), one Ilyonectria species (I. ilicicola sp. nov.), and one Neonectria species (N. quercicola sp. nov.) are newly described. The present study demonstrates the prevalence of this fungal group associated with seedlings of diverse hosts showing decline symptoms in forest nurseries in Spain.

Baorangia duplicatopora (Boletaceae, Boletales), a new bolete from tropical China

Phytotaxa ◽  
2021 ◽  
Vol 508 (1) ◽  
Author(s):  
XU ZHANG ◽  
ZHI-QUN LIANG ◽  
SHUAI JIANG ◽  
CHANG XU ◽  
XIN-HUA FU ◽  
...  
Keyword(s):  
New Species ◽  
Dna Sequences ◽  
Phylogenetic Analyses ◽  
Elongation Factor ◽  
Its Region ◽  
Tropical Region ◽  
Translation Elongation ◽  
Line Drawings ◽  
Microscopic Features ◽  
Tropical China

Baorangia duplicatopora is described as a new species from Hainan Province, a tropical region of China. It is morphologically characterized by large to very large basidiomata with a dull rose red, rose pink to purplish red pileus, compound pores, pileus context near hymenophore and stipe context staining blue when injured, a red stipe, and cheilocystidia wider than those of other Baorangia species. Phylogenetic analyses of DNA sequences from part of the 28S gene, the nuc rDNA internal transcribed spacer (ITS) region, and part of the translation elongation factor 1-α gene (TEF1) also confirmed that B. duplicatopora forms an independent lineage within Baorangia. Detailed descriptions, color photographs of fresh basidiomata, and line drawings of microscopic features of the new species are presented. A key to species of Baorangia in the world is also provided.

A new species of Scleroramularia associated with sooty blotch and flyspeck in Southern China

Phytotaxa ◽  
2015 ◽  
Vol 226 (3) ◽  
pp. 261 ◽  
Author(s):  
Liu Gao ◽  
Zhuoya Wei ◽  
Bo Wang ◽  
Mark L. Gleason ◽  
Rong Zhang ◽  
...  
Keyword(s):  
New Species ◽  
Southern China ◽  
Elongation Factor ◽  
Space Region ◽  
Translation Elongation ◽  
Sooty Blotch ◽  
Elongation Factor 1 Alpha ◽  
Sooty Blotch And Flyspeck ◽  
Elongation Factor 1 ◽  
A New Species

Scleroramularia is a genus that includes species of the sooty blotch and flyspeck (SBFS) fungal complex that blemishes the surface of apple, pawpaw, and other fruit. In a survey of SBFS on banana (Musa basjoo), an isolate associated with the flyspeck mycelial type of SBFS was obtained from Zhanjiang, Guangdong, China. Based on analysis of morphology and phylogeny (the nuclear ribosomal internal transcribed space region and translation elongation factor 1-alpha), it was delimited as a new species of Scleroramularia, described here as S. musae. Conidia of S. musae have more septa than other species presently known in the genus.

Sign in / Sign up

Export Citation Format

Share Document