scholarly journals Leaf epidermal micromorphology in Aspidistra (Asparagaceae): diversity and taxonomic significance

PhytoKeys ◽  
2021 ◽  
Vol 185 ◽  
pp. 65-86
Author(s):  
Nikolay A. Vislobokov ◽  
Long-Fei Fu ◽  
Yi-Gang Wei ◽  
Maxim S. Nuraliev

Micromorphological characters of leaf epidermis were investigated in 69 species of Aspidistra using scanning electron microscopy. Sculpture of epidermis varies from smooth to verrucose and rugose in the genus. The abaxial epidermis of some species bears papillae, whereas the adaxial surface uniformly lacks the papillae. Sculpture type of epidermis and density of papillae are generally found to be stable characters at a species level. The infraspecific variation of epidermis sculpture, where present, ranges from smooth to verrucose or from verrucose to rugose. Micromorphological characters of leaf epidermis are shown to have potential taxonomic significance in Aspidistra; in combination with the type of shoot structure, they allow to subdivide the species into 13 groups. The groups are largely incongruent with floral morphological traits. An identification key to the studied species of Aspidistra based on vegetative characters (gross leaf and shoot morphology and characters of leaf epidermis) is presented.

PhytoKeys ◽  
2020 ◽  
Vol 166 ◽  
pp. 1-28
Author(s):  
Agnieszka Rewicz ◽  
Jolanta Marciniuk ◽  
Paweł Marciniuk

The genus Taraxacum is one of the largest and taxonomically most complicated apomictic genera. Currently, it is considered to consist of over 2800 species placed within 60 sections. Due to the large number of species, and their uniform morphological design and plasticity of leaves, the identification of plants at the species level is challenging even for specialists. This problem significantly hinders the study of their properties and the rational use of these valuable medicinal and nutritional plants. This paper presents the results of research on the morphology and micromorphology of achenes of 28 Taraxacum species of sect. Palustria and for comparison one species per section of: Erythrosperma, Naevosa, Piesis, and Taraxacum. The achenes were measured with a stereoscopic microscope and a biometric program, and micromorphological studies were performed by scanning electron microscopy. It has been shown that traits associated with achene morphology and micromorphology have a high diagnostic value, allowing us to distinguish sections as well as species within the sect. Palustria. Based on the examined achene features, a dichotomous key for determining the studied species was constructed.


2014 ◽  
Vol 618 ◽  
pp. 344-348
Author(s):  
Ling Liu ◽  
Xi Tong Shen ◽  
Xiang Ping Chen ◽  
Xiao Min Fan ◽  
Ren Fang Chen

Study the Chinese Morus from the aspect of pollen morphology of 25 Chinese Morus plants of 10 species and 1 variety were observed using scanning electron microscopy (SEM). Results showed the differences to be mainly in pollen size, aperture size, P/E value and columellae structure.Supported the conclusion thatMorus bombycisis a species level unit butMorus mizuho,Morus atropurpurea, andMorus multicaulisare not, the conclusion thatMorus atropurpurea,Morus multicaulis, Morus alba, andMorus macrouraare initial forms, and thatMorus mongolica, Morus mongolicavar. diabolica, Morus bombycis, andMorus australisare later forms.


Bothalia ◽  
2015 ◽  
Vol 45 (1) ◽  
Author(s):  
Aluoneswi C. Mashau ◽  
Lyn Fish ◽  
Abraham E. Van Wyk

Background: Helictotrichon (excluding Amphibromus, Avenula, Helictochloa and Tricholemma), a genus of temperate C3 grasses, is represented by 14 species in southern Africa. Members of the genus are difficult to identify at species level on the basis of macromorphology alone.Objectives: The primary objective of this study was to investigate the usefulness of the micromorphology of the lemma surface for differentiating amongst the southern African members of Helictotrichon.Method: Lemma surfaces were studied with scanning electron microscopy (SEM). Lemmas were obtained from herbarium specimens housed in the National Herbarium, Pretoria (PRE). These were mounted on aluminium stubs using double-sided adhesive tape, sputter-coated with gold, and photographs were taken using a J840 scanning electron microscope.Results: Based on whether the lemma surface is smooth, papillate, scaberulous, scabrid or a combination of these, five groups of species are distinguished. All species, except Helictotrichon barbatum which has a smooth lemma surface, have small prickles (scaberulous). Helictotrichon leoninum, Helictotrichon quinquesetum and Helictotrichon rogerellisii have only small prickles, whilst Helictotrichon dodii, Helictotrichon hirtulum, Helictotrichon namaquense and Helictotrichon roggeveldense have, in addition, medium to large prickles (scabrid). Helictotrichon capense, Helictotrichon longifolium, Helictotrichon longum and Helictotrichon turgidulum have a combination of small prickles and papillae whilst Helictotrichon galpinii and Helictotrichon natalense have a combination of all three. A key to the groups and photos of the different types of surfaces are provided.Conclusion: The micromorphology of the lemma surface was shown to be of considerable taxonomic significance and to be extremely useful for differentiating amongst species.


1970 ◽  
Vol 16 (2) ◽  
pp. 177-180 ◽  
Author(s):  
Md. Tofazzal Islam ◽  
A.K.M. Golam Sarwar ◽  
Hasna Hena Begum ◽  
Toshiaki Ito

Not available.Keywords: Leaf epidermis; Rice; Scanning electron microscopy (SEM); Slender macro hair. DOI: 10.3329/bjpt.v16i2.3931 Bangladesh J. Plant Taxon. 16(2): 177-180, 2009 (December)


2015 ◽  
Vol 22 (2) ◽  
pp. 99-110 ◽  
Author(s):  
Maryam Naderifar ◽  
Ali Sonboli ◽  
Abbas Gholipour

Pollen morphology of 11 Iranian Dracocephalum L. species was investigated using light and scanning electron microscopy to evaluate their taxonomic significance for the infrageneric classification of the genus. Pollen grains of all examined taxa were isopolar, hexacolpate, circular in polar view and spheroidal to prolate in equatorial view (P/E = 1.0?2.0). The smallest pollen grains were observed in D. aucheri (P = 29.7 ?m, E = 22.6 ?m), while the largest pollen was found in D. lindbergii (P = 45.1 ?m, E = 33.7 ?m). The highest and lowest apocolpium index (AI) were measured in D. aucheri (AI = 0.27) and D. surmandinum (AI = 0.08), respectively. Colpus membrane was egranulate in all examined species except for D. multicaule and D. ghahremanii. The main exine ornamentation type was characterized as bireticulate including five different subtypes. The results revealed that the exine ornamentation is a diagnostic character useful for the classification of Dracocephalum.Bangladesh J. Plant Taxon. 22(2): 99-110, 2015 (December)


Botany ◽  
2020 ◽  
Author(s):  
Rafael Francisco Lopes-Silva ◽  
Anauara Lima Silva ◽  
Ednalva Alves Vital dos Santos ◽  
Maria de Fátima Agra

Bignonieae is the largest tribe of Bignoniaceae, with 21 genera and 393 species of lianas and shrubs, 2–3-foliolate with the terminal leaflet modified as tendrils. We examined the micromorphologies of the leaflet blade epidermises of 13 species of Bignonieae belonging to Amphilophium, Anemopaegma, Bignonia, Cuspidaria, Dolichandra, Fridericia, Pyrostegia, Tanaecium, and Xylophragma, from Pico do Jabre, Paraíba, Brazil. These are lianas except for Tanaecium parviflorum (shrubby). We sought to identify epidermal leaflet parameters to support their taxonomy subject to great similarities between their vegetative characters, mainly in species of the same genus and related genera. Analyses were performed using light and scanning electron microscopy, and showed five types of epicuticular waxes, four cuticle types, three epidermal cell anticlinal wall types, and non-glandular and glandular trichomes. Hypostomatic leaves showed ten different types of stomata, with stomatal indices from 6.21% (Bignonia ramentacea) to 23.52% (Tanaecium parviflorum) and stomatal densities from 76 stomata/mm² (Pyrostegia venusta) to 752.9 (T. parviflorum). The presence of raphides in Amphilophium crucigerum and styloids in Fridericia pubescens constitute the first records for these genera. Epidermal micromorphology provided a set of distinctive characters to separate these species, representing an additional tool to support their taxonomies, as well as that of tribe Bignonieae.


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