scholarly journals Resolving a taxonomic and nomenclatural puzzle in mantellid frogs: synonymization of Gephyromantis azzurrae with G. corvus, and description of Gephyromantis kintana sp. nov. from the Isalo Massif, western Madagascar

ZooKeys ◽  
2020 ◽  
Vol 951 ◽  
pp. 133-157
Author(s):  
Walter Cocca ◽  
Franco Andreone ◽  
Francesco Belluardo ◽  
Gonçalo M. Rosa ◽  
Jasmin E. Randrianirina ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
New Records ◽  
Molecular Data ◽  
The Other ◽  
Rrna Gene ◽  
Museum Specimens ◽  
Rich Family ◽  
Frog Species ◽  
The 16S Rrna Gene

The genus Gephyromantis belongs to the species-rich family Mantellidae and is currently divided in six subgenera. Among these is the subgenus Phylacomantis, which currently includes four described species: Gephyromantis pseudoasper, G. corvus, G. azzurrae, and G. atsingy. The latter three species are distributed in western Madagascar, and two of them (G. azzurrae and G. corvus) occur in the Isalo Massif. Based on the analysis of molecular data (a fragment of the 16S rRNA gene), morphological inspection of museum specimens, and photographic comparisons, G. azzurrae is synonymised with G. corvus and the second Phylacomantis lineage of Isalo is described as G. kintanasp. nov. This medium-sized frog species (adult snout-vent length 35–44 mm) is assigned to this subgenus according to genetic and morphological similarities to the other known species of Phylacomantis. Gephyromantis kintanasp. nov. is known only from the Isalo Massif, while new records for G. corvus extend its range to ca. 200 km off its currently known distribution. These two taxa seem to occur in syntopy in at least one locality in Isalo, and the easiest way to distinguish them is the inspection of the ventral colouration, dark in G. corvus and dirty white in G. kintana.

Phylogenetic relationships within Ovulidae (Gastropoda: Cypraeoidea) based on molecular data from the 16S rRNA gene

2005 ◽  
Vol 147 (2) ◽  
pp. 411-420 ◽  
Author(s):  
S. Schiaparelli ◽  
M. Barucca ◽  
E. Olmo ◽  
M. Boyer ◽  
A. Canapa
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Relationships ◽  
Molecular Data ◽  
Rrna Gene ◽  
The 16S Rrna Gene

Molecular data from the 16S rRNA gene for the phylogeny of Veneridae (Mollusca: Bivalvia)

2003 ◽  
Vol 142 (6) ◽  
pp. 1125-1130 ◽  
Author(s):  
A. Canapa ◽  
S. Schiaparelli ◽  
I. Marota ◽  
M. Barucca
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Data ◽  
Rrna Gene ◽  
The 16S Rrna Gene

Genetic and morphologic identities of hydrozoans (Cnidaria, Hydrozoa) from reef patches of Santa Marta, Colombian Caribbean

Zootaxa ◽  
2020 ◽  
Vol 4758 (1) ◽  
pp. 127-140
Author(s):  
JAVIER TORRES-RODRÍGUEZ ◽  
DANIEL ORTIZ-OYOLA ◽  
ALEJANDRA PUENTES-SAYO ◽  
ORLANDO P. LECOMPTE ◽  
AMINTA JAUREGUI
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Biological Diversity ◽  
New Records ◽  
Genetic Identification ◽  
Rrna Gene ◽  
Mitochondrial 16S Rrna Gene ◽  
The 16S Rrna Gene

The morphological and genetic identification of hydrozoans collected in the reef patches of Santa Marta, Colombia was carried out. This study allows to present two new records of hydroids species for the Colombian Caribbean: Halopteris alternata and Dentitheca dendritica. A total of 11 species and 1 genus were found using morphological and genetic identification with partial sequences of the mitochondrial 16S rRNA gene. The order Leptothecata was the most abundant represented by 9 families: Aglaopheniidae, Clytiidae, Haleciidae, Halopterididae, Kirchenpaueriidae, Plumulariidae, Sertularellidae, Sertulariidae and Thyroscyphidae, while the order Anthoathecata was represented by 2 families: Eudendriidae and Pennariidae. Despite the lack of studies on this group of organisms in the country, the use of the 16S rRNA gene proved to be very useful to provide complementary evidence in our understanding of the biological diversity of hydrozoans in Colombia. 

scholarly journals Myroides albus sp. nov., isolated from the gut of plastic-eating larvae of the coleopteran insect Zophobas atratus

2020 ◽  
Vol 70 (10) ◽  
pp. 5460-5466 ◽  
Author(s):  
Mengli Xia ◽  
Lin Hu ◽  
Yi-Xin Huo ◽  
Yu Yang
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Phylogenetic Analyses ◽  
The Other ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
Coleopteran Insect ◽  
Zophobas Atratus ◽  
The 16S Rrna Gene

A bacterial strain, BIT-d1T, was isolated from the gut of plastic-eating larvae of the coleopteran insect Zophobas atratus. Its taxonomic position was analysed using a polyphasic approach. Cells were white-pigmented, Gram-stain-negative, non-motile, long rods without flagella. The 16S rRNA gene sequence (1401 bp) of strain BIT-d1T showed highest similarity (98.0%) to Myroides pelagicus SM1T and 96.6~92.6 % similarity to the other species of the genus Myroides . The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of six housekeeping genes (gyrB, dnaK, tuf, murG, atpA and glyA) and genome sequences, placed strain BIT-d1T in a separate lineage among the genus Myroides , family Flavobacteriaceae . The major isoprenoid quinone was menaquinone-6 (MK-6) and the major fatty acids were C15 : 0 iso, C17 : 0 iso 3-OH and summed feature 9 (comprising iso-C17 : 1  ω9c and/or C16 : 0 10-methyl), which were similar to other members in the genus Myroides. In silico DNA–DNA hybridization and average nucleotide identity calculations plus physiological and biochemical tests exhibited the genotypic and phenotypic differentiation of strain BIT-d1T from the other members of the genus Myroides . Therefore, strain BIT-d1T is considered to represent a novel species within the genus Myroides , for which the name Myroides albus sp. nov is proposed. The type strain is BIT-d1T (=CGMCC 1.17043T=KCTC 72447T).

Desmonostoc danxiaense sp. nov. (Nostocales, Cyanobacteria) from Danxia mountain in China based on polyphasic approach

Phytotaxa ◽  
2018 ◽  
Vol 367 (3) ◽  
pp. 233 ◽  
Author(s):  
FANGFANG CAI ◽  
YIMING YANG ◽  
QIANZHI WEN ◽  
RENHUI LI
Keyword(s):  
New Species ◽  
16S Rrna ◽  
16S Rrna Gene ◽  
Southern China ◽  
Molecular Data ◽  
Rrna Gene ◽  
New Taxon ◽  
Cyanobacterial Species ◽  
Internally Transcribed Spacer ◽  
The 16S Rrna Gene

The recently established genus Desmonostoc was characterized by forming macroscopic colonies with diffluent mucilaginous envelopes embedding long vegetative filaments and possessing long chains of akinetes. The establishment of this new genus was further supported by the clustering of the 16S rRNA gene, which have a distinctive phylogenetic placement outside of Nostoc. In this study, a new cyanobacterial species was isolated from a wet rocky wall in Danxia mountain, Guangdong province, southern China, and the novel strains of this new species were evaluated by combining morphological characteristic and molecular data on the 16S rRNA gene and 16S-23S internally transcribed spacer (ITS). This new taxon was found to be closest to Desmonostoc species. The separation of the new species described here as Desmonostoc danxiaense, using morphological and molecular traits, was based on differences in phenotypic, 16S rRNA gene, ITS sequence and its secondary structure.

scholarly journals Identification of New Single Nucleotide Polymorphism-Based Markers for Inter- and Intraspecies Discrimination of Obligate Bacterial Parasites (Pasteuria spp.) of Invertebrates

2011 ◽  
Vol 77 (18) ◽  
pp. 6388-6394 ◽  
Author(s):  
Tim H. Mauchline ◽  
Rachel Knox ◽  
Sharad Mohan ◽  
Stephen J. Powers ◽  
Brian R. Kerry ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
The Other ◽  
Rrna Genes ◽  
Rrna Gene ◽  
Single Nucleotide ◽  
Content Type ◽  
Protein Encoding ◽  
Encoding Genes ◽  
The 16S Rrna Gene

ABSTRACTProtein-encoding and 16S rRNA genes ofPasteuria penetranspopulations from a wide range of geographic locations were examined. Most interpopulation single nucleotide polymorphisms (SNPs) were detected in the 16S rRNA gene. However, in order to fully resolve all populations, these were supplemented with SNPs from protein-encoding genes in a multilocus SNP typing approach. Examination of individual 16S rRNA gene sequences revealed the occurrence of “cryptic” SNPs which were not present in the consensus sequences of anyP. penetranspopulation. Additionally, hierarchical cluster analysis separatedP. penetrans16S rRNA gene clones into four groups, and one of which contained sequences from the most highly passaged population, demonstrating that it is possible to manipulate the population structure of this fastidious bacterium. The other groups were made from representatives of the other populations in various proportions. Comparison of sequences among threePasteuriaspecies, namely,P. penetrans,P. hartismeri, andP. ramosa, showed that the protein-encoding genes provided greater discrimination than the 16S rRNA gene. From these findings, we have developed a toolbox for the discrimination ofPasteuriaat both the inter- and intraspecies levels. We also provide a model to monitor genetic variation in other obligate hyperparasites and difficult-to-culture microorganisms.

scholarly journals Moraxella porci sp. nov., isolated from pigs

2010 ◽  
Vol 60 (10) ◽  
pp. 2446-2450 ◽  
Author(s):  
A. I. Vela ◽  
C. Sánchez-Porro ◽  
V. Aragón ◽  
A. Olvera ◽  
L. Domínguez ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Dna Hybridization ◽  
Molecular Data ◽  
Rrna Gene ◽  
16S Rrna Gene Sequences ◽  
Biochemical Tests ◽  
Physiological And Biochemical ◽  
The 16S Rrna Gene ◽  
Sequence Similarities

Nine Gram-negative, catalase- and oxidase-positive, coccus-shaped bacteria were isolated from pigs affected by different pathological processes. Phenotypic and genotypic methods were adopted to determine the relationships of these new isolates to recognized species of the genus Moraxella. Analysis of the 16S rRNA gene sequences demonstrated that the clinical isolates represented a new lineage within the genus Moraxella. The isolates were closely related to Moraxella cuniculi and Moraxella pluranimalium with 16S rRNA gene sequence similarities of 98.1 % and 99.1 %, respectively. The isolates displayed DNA–DNA relative binding ratios of 74 % to each other, but distinctly lower levels of DNA–DNA hybridization were observed with phylogenetically closely related moraxellae (<32 %). The new isolates could be distinguished from all other recognized species of the genus Moraxella by physiological and biochemical tests. On the basis of the phenotypic and molecular data, the nine new isolates from pigs represent a novel species within the genus Moraxella, for which the name Moraxella porci sp. nov. is proposed. The type strain is SN9-4MT (=CECT 7294T=CCUG 54912T).

Molecular Data from the 16S rRNA Gene for the Phylogeny of Pectinidae (Mollusca: Bivalvia)

2000 ◽  
Vol 50 (1) ◽  
pp. 93-97 ◽  
Author(s):  
Adriana Canapa ◽  
Marco Barucca ◽  
Annalisa Marinelli ◽  
Ettore Olmo
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Molecular Data ◽  
Rrna Gene ◽  
The 16S Rrna Gene

scholarly journals Phyllobacterium loti sp. nov. isolated from nodules of Lotus corniculatus

2014 ◽  
Vol 64 (Pt_3) ◽  
pp. 781-786 ◽  
Author(s):  
Maximo Sánchez ◽  
Martha-Helena Ramírez-Bahena ◽  
Alvaro Peix ◽  
María J. Lorite ◽  
Juan Sanjuán ◽  
...  
Keyword(s):  
16S Rrna ◽  
16S Rrna Gene ◽  
Sequence Similarity ◽  
Carbon Sources ◽  
Lotus Corniculatus ◽  
Culture Conditions ◽  
Rrna Gene ◽  
Content Type ◽  
Link Type ◽  
The 16S Rrna Gene

Strain S658T was isolated from a Lotus corniculatus nodule in a soil sample obtained in Uruguay. Phylogenetic analysis of the 16S rRNA gene and atpD gene showed that this strain clustered within the genus Phyllobacterium . The closest related species was, in both cases, Phyllobacterium trifolii PETP02T with 99.8 % sequence similarity in the 16S rRNA gene and 96.1 % in the atpD gene. The 16S rRNA gene contains an insert at the beginning of the sequence that has no similarities with other inserts present in the same gene in described rhizobial species. Ubiquinone Q-10 was the only quinone detected. Strain S658T differed from its closest relatives through its growth in diverse culture conditions and in the assimilation of several carbon sources. It was not able to reproduce nodules in Lotus corniculatus. The results of DNA–DNA hybridization, phenotypic tests and fatty acid analyses confirmed that this strain should be classified as a representative of a novel species of the genus Phyllobacterium , for which the name Phyllobacterium loti sp. nov. is proposed. The type strain is S658T( = LMG 27289T = CECT 8230T).

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