Marker-assisted selection for rice brown planthopper (Nilaparvata lugens)resistance using linked SSR markers

AbstractTo assess genetic diversity in populations of the brown planthopper (Nilaparvata lugensStål) (Homoptera: Delphacidae), we have developed and applied microsatellite, or simple sequence repeat (SSR), markers from expressed sequence tags (ESTs). We found that the brown planthopper clusters of ESTs were rich in SSRs with unique frequencies and distributions of SSR motifs. Three hundred and fifty-one EST-SSR markers were developed and yielded clear bands from samples of four brown planthopper populations. High cross-species transferability of these markers was detected in the closely related planthopperN. muiri. The newly developed EST-SSR markers provided sufficient resolution to distinguish within and among biotypes. Analyses based on SSR data revealed host resistance-based genetic differentiation among different brown planthopper populations; the genetic diversity of populations feeding on susceptible rice varieties was lower than that of populations feeding on resistant rice varieties. This is the first large-scale development of brown planthopper SSR markers, which will be useful for future molecular genetics and genomics studies of this serious agricultural pest.

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High-resolution mapping of a new brown planthopper (BPH) resistance gene, Bph 18(t), and marker-assisted selection for BPH resistance in rice (Oryza sativa L.)

Theoretical and Applied Genetics ◽

10.1007/s00122-005-0194-x ◽

2006 ◽

Vol 112 (6) ◽

pp. 1192-1194 ◽

Cited By ~ 1

Author(s):

K. K. Jena ◽

J. U. Jeung ◽

J. H. Lee ◽

H. C. Choi ◽

D. S. Brar

Keyword(s):

Oryza Sativa ◽

High Resolution ◽

Resistance Gene ◽

Marker Assisted Selection ◽

Oryza Sativa L ◽

Brown Planthopper ◽

High Resolution Mapping ◽

Resolution Mapping ◽

Selection For ◽

Bph Resistance Gene

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PRELIMNARY RESULTS ON MARKER ASSISTED SELECTION FOR DISEASE RESISTANCE IN COMMON WHEAT (TRITICUM AESTIVUM)

Bulletin of University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca Agriculture ◽

10.15835/buasvmcn-agr:1559 ◽

1970 ◽

Vol 62 ◽

Author(s):

C. Botez ◽

Monica Iuoraş ◽

P. Raica ◽

N. N. Saulescu

Keyword(s):

Triticum Aestivum ◽

Disease Resistance ◽

Bread Wheat ◽

Ssr Markers ◽

Resistance Genes ◽

Marker Assisted Selection ◽

Rapd Markers ◽

Issr Markers ◽

Resistant Lines ◽

Selection For

Our attempts to find RAPD markers linked with resistance genes to Septoria and Tilletia relying on co-segregation and to test the SSR markers, identified in literature, in order to select bread wheat resistant to Septoria and Tilletia are presented. Several candidate RAPD markers possibly linked with Septoria resistance genes and fewer linked with Tilletia resistance genes were identified. Some RAPD and SSR markers were specific for some resistant lines. These markers, probably, marked different resistance genes in different lines. It seems that some Septoria resistance genes came from rye genome because these genes were marked with some primers specific for rye genome. We found segregation for these markers in several lines, indicating that the analyzed lines were not completely stabilized. The ISSR markers used could not discriminate the resistant from susceptible genotypes to Septoria and Tilletia.

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