scholarly journals Oocyte recovery and in vitro embryo production in cows treated with a single dose of follicle-stimulating hormone

2020 ◽  
Vol 44 (1) ◽  
pp. 151-155
Author(s):  
Thiago Marquez FERNANDES ◽  
Paulo Roberto ADONA ◽  
Samuel GUEMRA ◽  
Tiago Henrique Camara DE BEM ◽  
Moysés dos Santos MIRANDA
Keyword(s):  
Single Dose ◽  
Follicle Stimulating Hormone ◽  
Embryo Production ◽  
Oocyte Recovery ◽  
In Vitro Embryo Production ◽  
Stimulating Hormone

scholarly journals Ovum Pick Up: Cows Treated With Single Doses of Follicle Stimulating Hormone

2019 ◽  
Vol 11 (10) ◽  
pp. 231 ◽  
Author(s):  
Bárbara Letícia Marchi da Silva ◽  
Paulo Roberto Adona ◽  
Samuel Guemra ◽  
Paulo Sergio Monzani ◽  
Moysés dos Santos Miranda
Keyword(s):  
Single Dose ◽  
Follicle Stimulating Hormone ◽  
Oocyte Retrieval ◽  
Ovarian Follicles ◽  
In Vitro Production ◽  
Oocyte Recovery ◽  
The Mean ◽  
Vitro Production ◽  
Stimulating Hormone

Cows treated with single doses of follicle stimulating hormone (FSH) and ovum pick up (OPU) after 24 hours were evaluated for oocyte recovery, in vitro production of embryos (IVPE), and transferred embryos. To begin evaluations, the ovarian follicles larger than three millimeters in diameters were removed from all cows used in the study. Two days after OPU, 200 milligrams of FSH was given in a single dose in 6 cows (treated). Twenty-four hours after application of FSH, the cows underwent a new OPU session for oocyte retrieval. These procedures were repeated three consecutive times without interval. In control (FSH-free) cows the OPU were performed at intervals of one week or oocyte retrieval. The viable oocytes were submitted to IVPE, and the blastocysts were transferred to the recipients. The mean number of oocytes did not differ (p > 0.05) between control cows (12.1±2.8) and those treated (10.9±1.6). There were also no differences (p > 0.05) in the number (6.6±1.7 and 7.1±0.9, respectively) or in the percentage (54.4±3.3 and 64.5±3.1%, respectively) of viable oocytes between control cows and those treated. The percentage of IVPE and pregnancy did not differ (p > 0.05) between control cows (39.8±2.6% and 44.7±4.8%) and those treated (37.8±2.5% and 39.5±4.1%), respectively. The treatment with single doses of FSH did not significantly alter the evaluated results for oocyte recovery, IVPE and transferred embryos, and, therefore, did not promote significant improvements for the IVPE as a whole.

In vitro embryo production using ovaries removed from culled cows

2001 ◽  
Vol 81 (4) ◽  
pp. 589-591 ◽  
Author(s):  
G. Giritharan ◽  
R. Rajamahendran
Keyword(s):  
Dairy Cows ◽  
Key Words ◽  
Estrous Cycle ◽  
Recovery Rate ◽  
In Vitro Maturation ◽  
Follicle Stimulating Hormone ◽  
Embryo Production ◽  
Oocyte Recovery ◽  
In Vitro Embryo Production

The objective was to utilize in vitro maturation and fertilization to maximize embryo production from culled dairy cows. Ovaries were removed (via a colopotomy technique) from culled Holstein cows (n = 21) 2 d after standing estrus (T1), 2 d after treatment with 40 mg follicle stimulating hormone (FSH) given at standing estrus (T2), or at an unknown stage of the estrous cycle (T3).Treatment with FSH increased (P < 0.05) oocyte recovery rate (mean ± SEM, 14.6 ± 2.2, 25.4 ± 3.4, and 11.1 ± 2.6 oocytes ovary–1), for groups T1, T2 and T3, respectively, and the number of blastocysts obtained (1.6 ± 0.5, 4.4 ± 0.4 and 1.6 ± 0.6 blastocysts ovary–1). Culled dairy cows of high genetic merit are potential embryo donors, with an average of six to eight transferable embryos obtained from each FSH-treated cow. Key words: FSH treatment, oocyte recovery, IVM, IVF, culled cows

135 Use and dose of porcine follicle-stimulating hormone for ovarian superstimulation prior to ovum pickup and in vitro embryo production in pregnant Holstein heifers

2019 ◽  
Vol 31 (1) ◽  
pp. 192
Author(s):  
R. V. Sala ◽  
L. C. Carrenho-Sala ◽  
M. Fosado ◽  
E. Peralta ◽  
D. C. Pereira ◽  
...  
Keyword(s):  
Limited Information ◽  
Dominant Follicle ◽  
Blastocyst Development ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Oocyte Competence ◽  
Treatment Groups ◽  
Oocyte Recovery ◽  
In Vitro Embryo Production

The benefit of superstimulation with exogenous FSH before ovum pickup for in vitro embryo production has been the subject of significant controversy. In addition, there is limited information on different dose regimens. Thus, the objective of the present study was to evaluate the effect of dose of porcine (p)-FSH during superstimulation before ovum pickup (OPU) on in vitro embryo production in pregnant heifers. Pregnant Holstein heifers (n=36) were assigned to a complete 3×3 crossover design. Three treatment groups were evaluated as follows: p-FSH 0mg (FSH0), p-FSH 160mg (FSH160) and p-FSH 300mg (FSH300). Three sessions of OPU were performed on each animal at 48, 62 and 76 days of gestation, with a washout interval between sessions of 14 days. Follicular wave emergence was synchronized by dominant follicle removal. Heifers in the FSH0 group received no further treatment, whereas the remaining groups received a total of 4 injections 12h apart as follows: FSH160 (48.0, 42.7, 37.3 and 32.0mg) or FSH300 (90.0, 80.0, 70.0 and 60.0mg), beginning 36h after dominant follicle removal. Ovum pickup was performed in all heifers 40h after the last p-FSH injection. Heifers were subjected to OPU for oocyte recovery, and number of follicles was determined. Recovered oocytes were processed and in vitro embryo production performed. Differences between treatment groups were evaluated by generalized linear mixed models. Data are presented (Table 1) as mean±standard error of the mean. There was no effect of days in gestation for any of the outcomes evaluated (P&gt;0.05). Follicle numbers at the time of oocyte recovery were different (P&lt;0.01) between groups. Heifers in the FSH300 group had a greater (P&lt;0.05) number of medium, large and total follicles than heifers in the FSH0 group, whereas heifers in the FSH160 were intermediate. Total number of recovered, viable and cleaved oocytes were greater (P&lt;0.01) in FSH300- than in FSH160- and FSH0-treated heifers. Cleavage rate and blastocyst development rate were not different (P&gt;0.10) between groups. The number of grade 1 and 2 blastocysts was greater in FSH300- than in FSH160- and FSH0-treated heifers (P&lt;0.03). In summary, the use of 300mg of p-FSH before OPU in pregnant heifers increases the number of follicles, oocytes and blastocysts produced per heifer with no detrimental effect on oocyte competence. Table 1.Ovum pickup and in vitro embryo production in pregnant heifers treated with different doses of porcine FSH

scholarly journals Effect of deslorelin acetate treatment in oocyte recovery and in vitro embryo production in domestic cats

2016 ◽  
Vol 19 (10) ◽  
pp. 1091-1095
Author(s):  
Camila Louise Ackermann ◽  
Eduardo Trevisol ◽  
Leticia Ferrari Crocomo ◽  
Tatiana da Silva Rascado ◽  
Rodrigo Volpato ◽  
...  
Keyword(s):  
Treated Group ◽  
Control Group ◽  
Domestic Cats ◽  
Cleavage Rate ◽  
Embryo Production ◽  
Oocyte Recovery ◽  
Significant Difference ◽  
In Vitro Embryo Production ◽  
Blastocyst Rate

Objectives The present study investigated the effect of contraceptive treatment with deslorelin acetate on in vitro embryo production and oocyte recovery in domestic queens. Methods Twenty-one mature domestic cats were used. Eleven queens (treated group) and one tom were kept in an experimental cattery, and 10 queens were privately owned (control group). When in interestrus or diestrus (day 0) a deslorelin acetate implant (Suprelorin, 4.7 mg/animal) was inserted into the subcutaneous tissue of the interscapular region in all queens in the treated group. After 6 months of treatment, all animals were ovariohysterectomized, and the ovaries were used for in vitro embryo production. Percentage of cleavage was determined 18 h after oocyte insemination and blastocyst formation was assessed on the eighth day of culture. The rate of cumulus-oocyte complexes (COCs) recovery was analyzed by an unpaired t-test. The cleavage and blastocyst rates were expressed as percentages and analyzed by Fisher’s exact test. All analyses were performed using GraphPad Prism v5.0, with P <0.05 set as the level of significance. Results In the treated group, we recovered 8.3 ± 1.15 grade I COCs per queen; the cleavage rate was 60% and the blastocyst rate was 36%. In the control group, we recovered 18.4 ± 3.21 grade I COCs per queen; the cleavage rate was 55.97% and the blastocyst rate was 34%. Forty percent of treated females did not produce any blastocysts. In the treated group, we observed a significant decrease in COC recovery. Although there was no significant difference in cleavage and blastocyst rates between groups, 40% of treated females did not produce any blastocysts. Conclusions Recovery of grade I COCs is negatively affected by deslorelin treatment in domestic cats. Regarding embryo production, new studies are still necessary to evaluate the success of this technique owing to the individual effect caused by deslorelin acetate.

In vivo oocyte recovery and in vitro embryo production from bovine donors aspirated at different frequencies or following FSH treatment

1999 ◽  
Vol 51 (5) ◽  
pp. 951-961 ◽  
Author(s):  
K.L Goodhand ◽  
R.G Watt ◽  
M.E Staines ◽  
J.S.M Hutchinson ◽  
P.J Broadbent
Keyword(s):  
Embryo Production ◽  
Oocyte Recovery ◽  
In Vitro Embryo Production

In vivo embryo recovery vs. in vivo oocyte recovery and in vitro embryo production: Embryo yields and pregnancy rates in cattle

1997 ◽  
Vol 47 (1) ◽  
pp. 364 ◽  
Author(s):  
K.L. Goodhand ◽  
R.G. Watt ◽  
M.E. Staines ◽  
L.C. Higgins ◽  
D.F. Dolman ◽  
...  
Keyword(s):  
Pregnancy Rates ◽  
Embryo Production ◽  
Embryo Recovery ◽  
Oocyte Recovery ◽  
In Vitro Embryo Production

scholarly journals Effect of single dose follicle stimulating hormone on follicular aspiration, in vitro fertilization and pregnancy rate

2019 ◽  
Vol 56 (3) ◽  
pp. e156894 ◽  
Author(s):  
Mayara Ellen Bardi de Moraes ◽  
Paulo Roberto Adona ◽  
Samuel Guemra ◽  
Tiago Henrique Camara De Bem ◽  
Moysés Dos Santos Miranda
Keyword(s):  
In Vitro Fertilization ◽  
Pregnancy Rate ◽  
Follicle Stimulating Hormone ◽  
Vitro Fertilization ◽  
Average Recovery ◽  
Follicular Aspiration ◽  
Oocyte Recovery ◽  
The Mean ◽  
Stimulating Hormone

The present study evaluated Brangus cows treated with single doses of follicle stimulating hormone (FSH) subjected to follicular aspiration after 24 h to assess oocyte recovery, in vitro fertilization and pregnancy rate. Follicles exceeding 3 millimeters in diameter were aspirated, 200 mg of FSH was administered 2 days later, and a new ovum pickup was performed 24 h afterward. These methods were performed 3 times every 3 days. In control, follicular aspirations occurred at intervals of 1-week without FSH administration o. The aspirated oocytes were evaluated, submitted to in vitro fertilization and the embryos were transferred to the recipients. The average recovery of oocytes was higher (p<0.05) in control cows (12.4±1.8) than in treated cows (9.4±1.3). There was no difference (p>0.05) in the mean percentage of viable oocytes (52.0±3.9 and 62.7±4.7%) or the mean percentage of embryos (41.4±4.8 and 41.5±4.2%) among control and treated cows, respectively. The mean percentage of pregnancy did not differ (p>0.05) for control cows (43.8±2.7%), and treated cows (40.9±6.8%). In conclusion, FSH treatment did not improve oocyte recovery, in vitro fertilization, and pregnancy percentage. However, there is possibility of several consecutive ovum pickup every t3 days, concentrating the in vitro fertilization and the pregnancy percentage.

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