Serum protein electrophoretic pattern in clinically healthy calves and cows

2013 ◽  
pp. 249-252
Author(s):  
Csilla Tóthová ◽  
Oskar Nagy ◽  
Gabriel Kováč ◽  
Veronika Nagyová
Keyword(s):  
Serum Protein ◽  
Electrophoretic Pattern ◽  
Protein Electrophoretic Pattern

scholarly journals Serum protein electrophoretic pattern in piglets during the early postnatal period

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Csilla Tóthová ◽  
Robert Link ◽  
Petronela Kyzeková ◽  
Oskar Nagy
Keyword(s):  
Serum Protein ◽  
Serum Proteins ◽  
Protein Pattern ◽  
Electrophoretic Pattern ◽  
Postnatal Period ◽  
Early Postnatal Period ◽  
Total Proteins ◽  
Newborn Piglets ◽  
Γ Globulins ◽  
Protein Electrophoretic Pattern

AbstractThe pattern of serum proteins, the typical features of the electrophoretogram in newborn piglets and during their postnatal development is not completely described. Therefore, the aim of this study was to characterize the changes in serum protein electrophoretic pattern and features of the electrophoretograms during the early postnatal period. Significant changes during the monitored period were found in all evaluated parameters (P < 0.001). The most marked changes were observed mainly in the period before weaning. The concentrations of total proteins, albumin and γ-globulins were before colostrum intake low, γ-globulins represented the smallest proportion of protein fractions. The proportion of α1-globulins was after birth a dominant protein fraction. Significant increase of total proteins, α2-, β- and γ-globulins and decrease of α1-globulins was found 2 days after colostrum intake. The albumin and A/G values increased after birth gradually until weaning. After weaning a significant changes were found in absolute concentrations of total protein and albumin, and in relative values of β-globulin fractions. Presented results showed marked developmental alterations in the serum protein pattern in piglets along with the age. The study also brings new knowledge in the field of description of typical features of electrophoretograms in the observed period of piglet’s life.

scholarly journals The effect of chronic respiratory diseases in calves on the serum protein electrophoretic pattern

2012 ◽  
Vol 81 (4) ◽  
pp. 365-370 ◽  
Author(s):  
Csilla Tóthová ◽  
Oskar Nagy ◽  
Herbert Seidel ◽  
Gabriel Kováč
Keyword(s):  
Serum Protein ◽  
Respiratory Diseases ◽  
Clinical Symptoms ◽  
Electrophoretic Pattern ◽  
Protein Fractions ◽  
Total Serum Protein ◽  
Total Serum ◽  
Chronic Respiratory Diseases ◽  
Γ Globulins ◽  
Protein Electrophoretic Pattern

The aim of this study was to evaluate the effect of chronic respiratory diseases in calves on the serum protein electrophoretic pattern. Twenty five calves of the Slovak Spotted breed, Low-land Black Spotted breed, and their crossbreeds with clinical symptoms of chronic respiratory diseases were included into this study. Blood serum was analyzed for the total serum protein concentrations, and the relative and absolute values of serum protein fractions. The results obtained in sick animals were compared with those in 29 clinically healthy calves of the same age and breed. In the calves suffering from chronic respiratory diseases, we found significantly (P < 0.001) higher total serum protein concentrations than in healthy calves. In sick calves a marked shift in the concentrations of the most of protein fractions was observed, with significantly higher values of α1-, β1-, β2- and γ-globulins (P < 0.001, P < 0.05, P < 0.001, and P < 0.01, respectively). On the other hand, the concentrations of albumin in the calves with respiratory diseases were significantly (P < 0.001) lower than those measured in healthy ones. The presented results indicate a marked effect of chronic respiratory diseases in calves on the serum protein electrophoretic pattern, with a shift in the albumin and globulin concentrations, which could be useful for clinicians for better evaluation of the pathological changes in affected animals.

scholarly journals Modulation of Serum Protein Electrophoretic Pattern and Leukocyte Population in Horses Vaccinated against West Nile Virus

Animals ◽  
2021 ◽  
Vol 11 (2) ◽  
pp. 477
Author(s):  
Francesca Arfuso ◽  
Elisabetta Giudice ◽  
Simona Di Pietro ◽  
Giuseppe Piccione ◽  
Claudia Giannetto
Keyword(s):  
West Nile Virus ◽  
Serum Protein ◽  
Electrophoretic Pattern ◽  
Vaccine Dose ◽  
West Nile ◽  
Dose Administration ◽  
Vaccine Booster ◽  
Baseline Values ◽  
Protein Electrophoretic Pattern ◽  
Leukocyte Populations

This study aimed to evaluate the hematological and serum protein electrophoretic profiles of horses after inactivated West Nile virus (WNV) vaccine administration. Blood samples were collected from 10 horses before (T0), after 24 h, 48 h, 72 h, 1 week, 2 weeks and 3 weeks (T1I, T2I, T3I, T4I, T5I and T6I) from the first WNV vaccine-dose administration, before the vaccine-booster (TPREII), and after 24 h, 48 h, 72 h, 1 week, 2 weeks and 3 weeks (T1I I, T2II, T3II, T4II, T5II, T6II) from the WNV vaccine-booster. There was a significant increase in lymphocytes and a decrease in neutrophils after both the first vaccine-dose and vaccine-booster administration compared to the baseline values (p < 0.01). Monocytes showed higher values after 72 h, 1 week and 2 weeks from the vaccine-booster (p < 0.01). Higher serum total protein values were found in horses after both the first vaccine-dose and booster administration (p < 0.05). α1-lobulins increased after the vaccine-booster with the highest levels measured at T4II (p < 0.05); α-2- and β-globulin fractions increased throughout the post-vaccine period compared to the baseline values (p < 0.05); and higher γ-globulin values were found before the vaccine-booster (TPREII) and after 24 h, 72 h and 3 weeks from the vaccine-booster (T1II, T3II and T6II). The findings allow us to conclude that the WNV vaccine used in the current study does not alter the overall hemogram picture of horses although it is associated with modulation of leukocyte populations and the serum protein electrophoretic pattern.

Comparative study on serum protein concentration and electrophoretic pattern in clinically healthy gazelles

2019 ◽  
Author(s):  
A. M. Gar-Elnabi ◽  
I. A. Alhidary ◽  
A. O. Bakhiet ◽  
K. A. Abdoun
Keyword(s):  
Serum Protein ◽  
Gel Electrophoresis ◽  
Protein Concentration ◽  
Electrophoretic Pattern ◽  
Normal Serum ◽  
Agarose Gel Electrophoresis ◽  
Protein Electrophoretic Pattern ◽  
Clinical Conditions ◽  
G Ratio ◽  
Serum Protein Concentration

Screening of electrophoretic pattern of serum protein is crucial for the initial evaluation of numerous clinical conditions. The present study aimed to investigate and compare the normal serum protein electrophoretic pattern in three gazelle species which included sand gazelle (Gazella gazella), mountain gazelle (Gazella subgutterosa) and Erlanger’s gazelle (Gazella erlangeri). Significant (P £ 0.05) interspecies differences were noticed in A/G ratio, and in á-1, â and globulins fractions using agarose gel electrophoresis. Significant (P £ 0.05) differences between males of the three species were encountered in á-1, á- 2 and â globulins fractions, while significant differences in A/G ratio, á-1, á-2 and â globulins fractions were observed between females of the three species. Significance (P £ 0.05) intersex differences were observed for albumin in sand gazelle, and for á-2 and â globulins fractions in mountain gazelle. The results obtained from the current study could provide a baseline references and could be used for diagnostic purposes.

scholarly journals Serum Protein Electrophoretic Pattern in Neonatal Calves Treated with Clinoptilolite

Molecules ◽  
2018 ◽  
Vol 23 (6) ◽  
pp. 1278 ◽  
Author(s):  
Simona Marc ◽  
Danijela Kirovski ◽  
Călin Mircu ◽  
Ioan Hutu ◽  
Gabriel Otavă ◽  
...  
Keyword(s):  
Serum Protein ◽  
Electrophoretic Pattern ◽  
Neonatal Calves ◽  
Protein Electrophoretic Pattern

scholarly journals Comparison of serum protein electrophoretic pattern in cows and small ruminants

2015 ◽  
Vol 84 (2) ◽  
pp. 187-195 ◽  
Author(s):  
Oskar Nagy ◽  
Csilla Tóthová ◽  
Veronika Nagyová ◽  
Gabriel Kováč
Keyword(s):  
Serum Protein ◽  
Adult Female ◽  
Serum Proteins ◽  
Electrophoretic Pattern ◽  
Small Ruminants ◽  
Protein Fractions ◽  
Total Serum Protein ◽  
Total Serum ◽  
Γ Globulins ◽  
Protein Electrophoretic Pattern

Determination of the physiological electrophoretic patterns in animals is very useful for clinicians in diagnosing healthy and sick animals. The objective of this study was to investigate the serum protein electrophoretic pattern in cows, sheep, and goats in order to evaluate the differences in the size and number of protein fractions between the evaluated ruminant species. Ten adult multiparous high-yielding dairy cows, 10 adult female sheep and 10 adult female goats were included in this study. All the evaluated animals were clinically healthy. Serum was analyzed for total serum protein concentrations, and for the relative and absolute values of protein fractions with calculation of albumin/globulin ratios. Serum protein fractions were separated by zone electrophoresis on buffered agarose gel. Serum protein electrophoresis identified 6 distinct bands, comprising albumin, alpha1- (α1), alpha2- (α2), beta1- (β1), beta2- (β2), and gamma- (γ) globulins in cows. In sheep, serum proteins exhibited 6 fractions: albumin, α1-, α2-, β-, γ1- and γ2-globulins. In goats, serum proteins were separated into 5 fractions: albumin, α1-, α2-, β- and γ-globulins. Significant differences in the relative as well as absolute means were found for the albumin/globulin ratio and most of the protein fractions, except γ-globulins. No significant differences were found in the concentration of total proteins. These results describe the marked species differences in most of serum protein fractions between the evaluated groups of animals, and contribute to the current knowledge about the physiological electrophoretic pattern of serum proteins in ruminants, which can be used for diagnostic purposes.

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