2.3. Development of a multi-Kinect-system for gait analysis and measuring body characteristics in dairy cows

Individual and combined effects of several isomers of retinoic acid (RA) and 1,25-dihydroxyvitamin D3 (1,25-(OH)2D3) on interferon-gamma (IFN-gamma) secretion by blood mononuclear leukocytes (MNL) from nulliparous and postparturient Holstein cattle were evaluated in vitro. In the first experiment, effects on incubation period (24 to 72 hours) and time of supplementation (0 to 32 hours) with all-trans, 9-cis, 13-cis-, and 9,13-dicis-RAs (0 to 100 nM) on IFN-gamma secretion by pokeweed mitogen (PWM)-stimulated (0 and 10 mug/ml) MNL from nulliparous cattle were evaluated. In the second experiment, MNL from postparturient cows (bled at 0, 2, 4, and 16 days postpartum) were stimulated with PWM (0 and 10 mug/ml) in the presence of RA isomers (9-cis- or 9,13-dicis-RA; 0 to 100 nM), 1,25-(OH)2D3 (0 to 100 nM), or with combinations of these metabolites. The results show that individual isomers of RA had no effect on IFN-gamma secretion by PWM-stimulated MNL from nulliparous or postparturient cows. Furthermore 1,25-dihydroxyvitamin D3 inhibited IFN-gamma secretion by MNL from nulliparous and postparturient dairy cows; however, the degree of inhibition was greater when 9-cis- and 9,13-dicis-RA were also present in the cultures. Finally mononuclear leukocytes from postparturient dairy cows produced substantially less IFN-gamma than did MNL from nulliparous cattle. It is concluded that retinoic acids individually did not affect the capacity of leukocytes from dairy cattle to secrete IFN-gamma. This result is in marked contrast to studies in monogastric species indicating that RAs inhibit IFN-gamma secretion by peripheral blood T cells. Inhibition of IFN-gamma secretion by 1,25-(OH)2D3 was potentiated by 9-cis- and 9,13-di-cis-retinoics acids, suggesting that an excess of dietary vitamins A and D may compromise further the naturally immunosuppressed postparturient dairy cow. Additional research is necessary to determine if the combined effects of these metabolites on IFN-gamma secretion represent an increased susceptibility of the dairy cow to infectious diseases during the periparturient period. Lower secretion of IFN-gamma by MNL from postpartutient dairy cows, relative to nulliparous cattle, suggests that recently-calved cows are naturally immunosuppressed.

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Sensitivity of gait analysis to detect motor phenotype in pre-manifest and manifest Huntington's disease – cross-sectional results from the TRACK-HD Study

Aktuelle Neurologie ◽

10.1055/s-0029-1238520 ◽

2009 ◽

Vol 36 (S 02) ◽

Author(s):

S Rohm ◽

N Bechtel ◽

H Beckmann ◽

A Sturrock ◽

S van den Bogaard ◽

...

Keyword(s):

Gait Analysis ◽

Huntington's Disease ◽

Huntington’S Disease ◽

Cross Sectional ◽

Motor Phenotype

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Influence of the herbal component of a commercial feed additive on serum parameters, fertility and longevity of dairy cows

Planta Medica ◽

10.1055/s-0030-1264919 ◽

2010 ◽

Vol 76 (12) ◽

Author(s):

M Walkenhorst ◽

S Ivemeyer ◽

J Spranger ◽

G Arndt ◽

R Schaette

Keyword(s):

Dairy Cows ◽

Feed Additive ◽

Serum Parameters ◽

Commercial Feed

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Gait Analysis

Physikalische Medizin Rehabilitationsmedizin Kurortmedizin ◽

10.1055/s-0030-1267628 ◽

2010 ◽

Vol 20 (05) ◽

Author(s):

A Delarque

Keyword(s):

Gait Analysis

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Relation between intravascular electrolyte level and course of parturition in dairy cows

Tierärztliche Praxis Ausgabe G Großtiere / Nutztiere ◽

10.1055/s-0038-1623185 ◽

2013 ◽

Vol 41 (05) ◽

pp. 289-296 ◽

Cited By ~ 2

Author(s):

K. Seyrek-Intas ◽

K. Failing ◽

G. Yilmazbas Mecitoglu ◽

H. Bostedt ◽

D. Seyrek-Intas

Keyword(s):

Dairy Cows ◽

Inorganic Phosphate ◽

Post Partum ◽

Total Calcium ◽

Normal Delivery ◽

P Values ◽

Normal Birth ◽

Factors Influencing ◽

Electrolyte Homeostasis ◽

Convalescence Phase

Summary Objective: To determine the intravascular electrolyte status in dairy cows with respect to age and different courses of parturition to clarify etiological factors influencing peri- or intrapartal imbalances of electrolyte homeostasis. Material and methods: A total of 64 cows at birth were evaluated (primiparous n = 34, pluriparous n = 30). Thirty-three cows showed normal delivery, while 31 cows had a complicated birth. Blood samples were collected intra partum (i. p.) and 2, 4, 6, 8, 12, 16, 24 h post partum (p. p.) as well as [2, 3, 4, 5, 7, 10 days p. p. and levels of total calcium (Catotal), ionized calcium (Caion), inorganic phosphate (Pa), Mg, Na, K, Cl were determined. Results: The results revealed that electrolytes show great fluctuation during and immediately p. p. in relation to age. Already during parturition pluriparous cows had a lower Catotal and Pa concentration compared to primiparous animals. Cows with dystocia exhibited a more intensive and longer lasting decrease of Ca compared to cows with normal birth. In relation to age and severity of birth Pa concentration showed a differing but basically typical course for this electrolyte. Mg, Na, K and Cl concentrations were higher during and immediately after birth compared to p. p. values. Until day 10 p. p. these electrolyte concentrations declined more in older cows with dystocia compared to younger animals. However, the influence of dystocia on concentration of these electrolytes was milder in contrast to Ca and Pa. Conclusions: In summary, primarily older cows are predisposed to imbalances of electrolyte homeostasis intra partum and at the beginning of the lactation. These changes are potentiated in case of complications during parturition. Intravascular Catotal, Caion as well as Pa are most severely affected. Clinical relevance: These results may constitute the basis for a comprehensive metaphylaxis during the peripartal period, especially in cows after dystocia, to positively influence the early convalescence phase.

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Force Plate Analysis of Dogs with Bilateral Hip Dysplasia Treated with a Unilateral Triple Pelvic Osteotomy: A Long-term Review of Cases

Veterinary and Comparative Orthopaedics and Traumatology ◽

10.1055/s-0038-1632616 ◽

1998 ◽

Vol 11 (02) ◽

pp. 85-93 ◽

Cited By ~ 10

Author(s):

Joanne R. Cockshutt ◽

H. Dobson ◽

C. W. Miller ◽

D. L. Holmberg ◽

Connie L. Taves ◽

...

Keyword(s):

Gait Analysis ◽

Hip Dysplasia ◽

Weight Bearing ◽

Force Plate ◽

Case Series ◽

Pelvic Osteotomy ◽

Vertical Force ◽

Long Term Outcome ◽

Case Series Study

SummaryA retrospective case series study was done to determine the long-term outcome of operations upon dogs treated for canine hip dysplasia by means of a triple pelvic osteotomy (TPO). Twentyfour dogs with bilateral hip dysplasia, that received a unilateral TPO between January 1988 and June 1995, were re-examined at the Ontario Veterinary College. The assessment included physical, orthopedic and lameness examinations, standard blood work, pelvic radiographs and force plate gait analysis. They were compared to bilaterally dysplastic dogs that had not been treated, and also to normal dogs. Force plate data analysis demonstrated a significant increase in peak vertical force (PVF) and mean vertical force over stance (MVF) in the limb that underwent surgical correction by means of a TPO, when compared to the unoperated hip. It was determined that performing a unilateral TPO on a young dysplastic dog resulted in greater forces and weight bearing being projected through the TPO corrected limb when compared to the unoperated limb.Dogs with bilateral hip dysplasia treated with a unilateral triple pelvic osteotomy (TPO) were assessed by force plate gait analysis, radiographs and orthopedic examination. There was a significant increase in hip Norberg angles over time, although degenerative changes did progress. Limbs that had been operated upon had significantly greater peak and mean ground reaction forces than limbs that had not received an operation.

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