Consequential study on different levels of C/N ratios used in biofloc-based aquaculture system

Biofloc technology is much highlighted these days because of its tremendous effects on aquaculture. Microbes were enriched on cheapest organic carbon source i. e., powdered banana peels and were incorporated in different aquaria rearing grass carp fingerlings under different C/N treatments (10:1, 15:1 and 20:1) and 10% water daily water exchange. The initial growth of fingerlings was recorded. The experiment was settled in triplicates for 60 days and run parallel to control group provided with commercial feed and daily water exchange. Its effect was evaluated by measuring the growth of fingerlings and water parameters of each aquarium. The average % gain in weight and length of fingerlings was obtained significantly highest (28.12 ± 0.30g and 17.29 ± 0.46cm respectively) in aquaria containing pure powdered banana peels with 10% water exchange and C/N ratio was adjusted at 20: 1 (T3) than other treatments and control. Ammonia and other water parameters were also under control in T3 than other experimental and control groups. By all counts, it was concluded that the highest C/N ratio in biofloc system had the potential to increment C. idella growth rate by reducing toxicity and could be used as fish meal substitute.

Evaluating the Influence of Different Recommended Dietary Levels of Cu and Zn on Finishing Pigs

This study was conducted to evaluate the effects of dietary supplementation of different recommended levels of Cu and Zn on antioxidant capacity, tissue mineral status, minerals excretion, meat quality, digestive enzyme activity, and metal transporters in finishing pigs. A total of 120 pigs (with an average initial body weight (BW) of 70.0 ± 2.1 kg) were randomly divided into four treatments: (1) basal diet without added Cu or Zn (control), (2) basal diet+35 mg cupreous N-carbamylglutamate chelate (NCG-Cu) +150 mg zinc-methionine chelate (Zn-Met) (AC), (3) basal diet + 3.0 mg of NCG-Cu + 43 mg Zn-Met (CN), and (4) basal diet + 3.5 mg NCG-Cu + 50 mg Zn-Met (NRC100). Pig growth performance was not affected by the level of Cu or Zn. Among the four treatments, the AC treatment had the highest concentration (P < 0.05) of glutathione peroxidase (GSH-Px). Pigs fed the AC diet had the highest (P < 0.05) liver Zn, fecal Cu, and fecal Zn among the four treatments. The protein levels of trypsin and aminopeptidase N (APN) in the intestinal mucosa showed their highest levels (P < 0.05) in the NRC100 and AC treatments. The mRNA levels of trypsinogen and APN were significantly up-regulated (P < 0.05) in the AC, CN, and NRC100 treatments compared with the control. The mRNA levels for the Zn transporter genes SLC30A1 (ZnT1) and SLC30A2 (ZnT2) were significantly up-regulated (P < 0.05) in the AC treatment, and the mRNA levels for SLC39A4 (ZIP4) and metallothionein 1 (MT) in the AC, CN, and NRC100 treatments were significantly up-regulated (P < 0.05) compared with the control. Meat quality were not affected (P > 0.05) by the different recommended levels of Cu and Zn. These results indicated that the supplemental Cu and Zn levels routinely used in AC diets in Chinese commercial feed enterprises should be reduced.

SENSORY QUALITY OF QUAIL EGGS FED WITH CORIANDER FLOUR (CORIANDUM SATIVUM LINN) IN THE FEED

One of the sources of animal protein for the community can be obtained from quail eggs. Quail eggs have nutritional value that is not inferior to other poultry eggs. Feed is one of the factors that can affect egg quality. Coriander is a spice that contains essential oils that are beneficial for the body when consumed. So it is hoped that the presence of this essential oil can help improve livestock growth and produce quality quail eggs sensory. This study aimed to examine the effect of giving coriander flour to quail feed on the sensory quality of eggs. The study was conducted for 30 days. The livestock used were Japanese quail aged 30 days with 100 heads. The feed used is commercial feed and additional coriander flour. The design used was a completely randomized design with 4 treatments and 5 replications for each unit of 5 birds with treatment R0 = commercial feed +0% coriander flour, R1 = commercial feed + 0.5% coriander flour, R2 = commercial feed + 1% coriander flour, R3 = commercial feed + 1.5% coriander flour. Data were analyzed using Kruskall Wallis and Duncan's multiple range test. The results showed that the administration of coriander flour had a significant effect (P<0.05) on the hedonic test of egg white color and texture hedonic quality test, while for the egg yolk color, taste, aroma variables were not significantly different (P>0.05). This study concludes that organoleptic administration of 1% coriander flour can increase the panelists' preference for egg whites and improve the texture of quail eggs.

EFFECT OF ADDITION OF TEMULAWAK EXTRACT (Curcuma xanthorriza) ON ARTIFICIAL FEED ON GURAMI FISH GROWTH (Osphronemus goramy)

The problem with gouramy cultivation was slow growth due to the short intestines of gouramy so that the absorption of feed takes longer and less. Several previous studies used temulawak in the form of flour, extract, oil which was applied to carp, tilapia, milkfish, catfish. The addition of extract, temulawak flour to the feed gave an increase in growth both in length and weight in the test fish. The purpose of this study was to determine whether or not the effect of temulawak extract on the growth of gouramy fish. This research was conducted for 45 days. This research was conducted by experimental method using a completely randomized design (CRD) with 5 treatments (P) 3 times replication (U), in order to obtain 15 experimental units. Treatment 0 Control (without temulawak extract), Treatment 1 Provision of temulawak extract with a concentration of 14%/kg feed, Treatment P2 Administration of temulawak extract with a concentration of 16%/kg feed, treatment P3 Administration of temulawak extract with a concentration of 18%/kg feed, and treatment P4 administration of temulawak extract with a concentration of 20%/kg feed. The results showed that in the P0 treatment the absolute weight gain was 0.78 grams, in the P1 treatment the absolute weight was 0.58 grams, the P2 treatment was 0.33 grams, the P3 treatment was 0.37 grams, and in the P4 treatment it was obtained The result of absolute weight gain was 0.39 grams. The results showed the absolute length increase ranged from 0.35-0.57 cm. It can be concluded that the addition of temulawak extract to the commercial feed of carp has no significant effect on absolute weight, absolute length, survival rate and feed efficiency level.

DETERMINING THE OPTIMUM TEMPERATURE FOR GROWTH, FEED EFFICIENCY AND SURVIVAL OF DOMESTICATED INDONESIAN MAHSEER, Tor soro LARVAE

Temperature plays a pivotal role in the success of larvae production. Evaluation of the effects of different temperatures on growth, feed efficiency, and survival of domesticated Indonesian mahseer (Tor soro) larvae was carried out to determine an optimum rearing temperature. Five different temperature settings of 22°C, 24°C, 26°C, 28°C, and 30°C were used as the treatments arranged in triplicates, in indoor closed recirculating systems. The larvae were stocked in a rectangular glass tank with a stocking density of 150 larvae per tank and fed with a commercial feed (30% protein content) three times a day, about 10% per body weight over 61 days of observation. Measured parameters included the specific growth rate of total weight (SGRTW) and length (SGRTL), feed efficiency (FE), and survival rate (SR). The result showed that the treatments of different temperatures significantly affected the growth performance and feed efficiency of the larvae. The growth performance and feed efficiency of mahseer larva were much better at rearing temperature from 24°C to 28°C compared with those at 22°C and 30°C. There were no significant differences in SR in different temperatures tested. The SR was found to range from 86.44% to 100% in all treatments. Maintaining temperature ranging from 24°C to 28°C is the best rearing condition to achieve the optimum growth and feed efficiency of mahseer during the larval rearing period. The findings from this research could be set as a standard technique in larval rearing of Indonesian mahseer (Tor soro).

Solid-State Hydrolysis (SSH) Improves the Nutritional Value of Plant Ingredients in the Diet of Mugil cephalus

The possibility of improving the nutritional quality of plant byproducts (brewers’ spent grain and rice bran) through an enzyme treatment was tested in a formulated feed for grey mullet (Mugil cephalus). The enzyme treatment was carried out by Solid-State Hydrolysis (SSH) using a commercial preparation including carbohydrases and phytase. A feed prepared without the treatment and a commercial feed for carp were used as controls. In a preliminary short-term trial carried out at laboratory facilities, fish receiving the enzyme-treated feed showed significant improvement in both FCR and SGR when compared to those obtained with the untreated diet, although both experimental diets presented worse values than those obtained with the commercial feed. Different metabolic indicators including higher values of muscle glycogen and plasmatic triglycerides supported the positive effect of the enzyme treatment on the nutritional condition of the fish over those fed on the diet containing non-treated ingredients. Results of growth and feed efficiency that were obtained in a second long-term trial developed for 148 days under real production conditions evidenced the equivalence among the experimental and commercial diets and confirmed that enzyme pretreatment of plant ingredients by SSH may be a useful procedure to improve the nutritive value of high fiber plant byproducts when included in practical diets for this species and others with similar nutritional features.

Performance of Different Biomaterials as Carbon Sources on the Immunological Response and Oxidative Status of African Catfish Clarias gariepinus in Biofloc Systems

A trial was performed to investigate how carbon sources can affect the welfare status of African catfish (3.8±0.2g and 8±0.11 cm) juveniles in biofloc systems. Inocula was prepared in glass tanks (5L) by adding 20g of pond bottom soil in well aerated water (1L) containing 10mg L-1 ammonium sulphate (NH)4SO4 and 400mg L-1 of four different carbon sources (tapioca, wheat offal, brewery and cassava peel) for 24 hours. Each treatment group (carbon sources and control) were replicated and each tank contained 1000 Clarias gariepinus juvenile, fed with commercial feed (Crude protein 42%) at 5% of their body weight 72 days. The water quality showed that all water parameters remained at concentrations suitable for Clarias gariepinus culture in the studied systems. The enzymes activities were noticed to be different across the biofloc and the selected organs. There were significant differences in serological content in fish between the treatment’s groups (P<0.05). The significant difference was found between the treatments in case of enzymes activity (P<0.05). The study shows that the welfare status with reference to digestive enzymes activity, oxidative status and extent of the immune system stimulation in BFT system is carbon source dependent.

Sifat Fisik Daging Kelinci Peranakan New Zealand White yang Diberi Tepung Kulit Pisang Kepok (Musa acuminata x Musa balbisiana)

This study aims to analyze the effect of Kepok banana peel flour (Musa acuminata x Musa balbisiana) in the ration on the physical of New Zealand White crossbreed rabbits meat. This research was conducted in the rabbit cage of Lampung State Polytechnic with 20 male rabbits of the New Zealand White breed aged 2 months. The experiment was carried out with an experimental method, using a completely randomized design (CRD), with 5 treatments and 4 replications. The treatments used in this study were P0: 100% commercial feed, P1: 98% commercial feed + 2% kepok banana peel flour, P2: 96% commercial feed + 4% kepok banana peel flour, P3: 94% commercial feed + 6 % Kepok banana peel flour, P4: 92% commercial feed + 8% Kepok banana peel flour. The results of statistical analysis showed that the pH value of the meat, cooking loss and tenderness of the meat had a significant effect. The results of statistical analysis showed that the value of the water holding capacity had no significant effect. The conclusion of this study is that feed fed with Kepok banana peel flour has a significant effect on the physical of New Zealand White crossbreed rabbit meat including the pH value of the meat with the lowest value of 5.70, at 2% treatment, loss of cooking meat with a value of 35.00 (% ) was found in treatment 4%, and meat tenderness with a value of 3.30 (gf) at 4% treatment and 3.20 (gf) at treatment 8%. However, it has no significant effect on water holding capacity.