In vitro Callus Induction and Shoot Multiplication from Nodal Explants and Leaves of Memecylon edule

2010 ◽  
Vol 2 (2) ◽  
pp. 110-119 ◽  
Author(s):  
Tamizhamudu Elavazhaga ◽  
Kantha D. Arunach
Keyword(s):  
Callus Induction ◽  
Shoot Multiplication ◽  
Nodal Explants

scholarly journals Shoot Multiplication and Callus Induction of Labisia pumila var. alata as Influenced by Different Plant Growth Regulators Treatments and Its Polyphenolic Activities Compared with the Wild Plant

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3229
Author(s):  
Mat Yunus Najhah ◽  
Hawa Z. E. Jaafar ◽  
Jaafar Juju Nakasha ◽  
Mansor Hakiman
Keyword(s):  
Callus Induction ◽  
Antioxidant Activities ◽  
Shoot Multiplication ◽  
Wild Plants ◽  
Wild Plant ◽  
Total Phenolic ◽  
Nodal Segment ◽  
Ms Medium ◽  
In Vitro Plantlets

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2,4-D) and picloram) for callus induction, respectively. The results showed that 2 mg/L zeatin produced the optimal results for shoot and leaf development, and 0.5 mg/L 2,4-D produced the highest callus induction results (60%). After this, 0.5 mg/L 2,4-D was combined with 0.25 mg/L cytokinins and supplemented to the MS medium. The optimal results for callus induction (100%) with yellowish to greenish and compact texture were obtained using 0.5 mg/L 2,4-D combined with 0.25 mg/L zeatin. Leaves obtained from in vitro plantlets and wild plants as well as callus were extracted and analyzed for their antioxidant activities (DPPH and FRAP methods) and polyphenolic properties (total flavonoid and total phenolic content). When compared with leaf extracts of in vitro plantlets and wild plants of L. pumila var. alata, the callus extract displayed significantly higher antioxidant activities and total phenolic and flavonoid content. Hence, callus culture potentially can be adapted for antioxidant and polyphenolic production to satisfy pharmaceutical and nutraceutical needs while conserving wild L. pumila var. alata.

scholarly journals Assessment of In-vitro culture through Nodal explants of Dendrocalamus hamiltonii

2021 ◽  
Vol 2 (1) ◽  
pp. 130-133
Author(s):  
Abha Jha ◽  
◽  
Sunila Das ◽  
Keyword(s):  
Experimental Study ◽  
Growth Regulators ◽  
In Vitro Regeneration ◽  
Shoot Multiplication ◽  
Growth Hormones ◽  
Nodal Explants ◽  
Bud Break ◽  
Multiplication Rate ◽  
Dendrocalamus Hamiltonii

The present experimental study was aimed to overcome the traditional methods of propagation that limit the number of propagules by in-vitro regeneration through nodal explants of Dendrocalamus hamiltonii with a comparative study of growth regulators during the shooting and rooting process. Dendrocalamus hamiltonii is distributed from the Himalayas (Nepal) to the northern part of Burma. Collection of explants was done from different selected sites of CPTs. There was the use of HgCl2 and Ca (OCl)2 as sterilizing agents in different concentrations and its effect was visualized during the sprouting stage. Culm explants were cultured in a bottle containing White media (Wm) supplemented with BA and Kinetin for sprouting and IAA, IBA, NAA for rooting. There is also the use of IAA+IBA+NAA in combined form as a supplementary solution 0.1% HgCl2 treatment for 20-minute results into77.80% aseptic buds and 72% bud -break. Among the used growth-hormones, BA with concentration 0.25mg/l and 0.50mg/l respectively were appropriate for shoot-multiplication rate, 4.01±0.3 and 4.3±0.4 were ideal observation incorporation with BA (1.00mg/l) and BA (1.50mg/l) respectively. Maximum sprouting rate14.77±3.37with application of BA (2.00mg/l) and maximum shoot length4.3±0.4 is observed at BA (1.50mg/l). The applications of rooting hormone IAA+IBA+NAA in the concentration of 1.0 mg/l results in 72.5±0.3(rooting) and 11.1±0.3 (av. No. of the root).

scholarly journals Micropropagation of Clerodendrum phlomidis L.F.

2016 ◽  
Vol 24 (1) ◽  
pp. 21-28 ◽  
Author(s):  
Mafatlal M. Kher ◽  
Deepak Soner ◽  
Neha Srivastava ◽  
Murugan Nataraj ◽  
Jaime A. Teixeira da Silva
Keyword(s):  
Callus Formation ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Nodal Explants ◽  
Axillary Shoot ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Axillary Shoot Proliferation ◽  
Clerodendrum Phlomidis

Abstract Clerodendrum phlomidis L. f. is an important medicinal plant of the Lamiaceae family, particularly its roots, which are used for various therapeutic purposes in a pulverized form. The objective of this study was to develop a standard protocol for axillary shoot proliferation and rooting of C. phlomidis for its propagation and conservation. Nodal explants were inoculated on Murashige and Skoog (MS) medium that was supplemented with one of six cytokinins: 6-benzyladenine, kinetin, thidiazuron, N6-(2-isopentenyl) adenine (2iP), trans-zeatin (Zea) and meta-topolin. Callus induction, which was prolific at all concentrations, formed at the base of nodal explants and hindered shoot multiplication and elongation. To avoid or reduce callus formation with the objective of increasing shoot formation, the same six cytokinins were combined with 4 μM 2,3,5-tri-iodobenzoic acid (TIBA) alone or in combination with 270 μM adenine sulphate (AdS). Nodal explants that were cultured on the medium supplemented with 9.12 μM Zea, 4 μM TIBA and 270 μM AdS produced significantly more and longer shoots than on medium without TIBA and AdS. Half-strength MS medium supplemented with 8.05 μM α-naphthaleneacetic acid was the best medium for root formation. Most (75%) in vitro rooted plantlets were successfully acclimatized under natural conditions.

scholarly journals In vitro selection of salt tolerant Morus alba and its field performance with bioinoculants

2011 ◽  
Vol 33 (No. 2) ◽  
pp. 77-86 ◽  
Author(s):  
S. Kashyap ◽  
S. Sharma
Keyword(s):  
In Vitro Selection ◽  
Field Performance ◽  
Shoot Multiplication ◽  
Arbuscular Mycorrhizal ◽  
Am Fungi ◽  
Morus Alba ◽  
Nodal Explants ◽  
Salt Tolerant ◽  
Indolebutyric Acid

&nbsp;In vitro selected salt tolerant saplings of Morus alba (cv. Sujanpuri) were raised from nodal explants with axillary buds collected during three different periods of the year. The growth and shoot/root multiplication of the nodal explants collected between November to February and July to October were found to be better than those collected between March to June. In cultures, shoot multiplication was induced by the application of 2.5 mg/l of 6-benzylaminopurine (BAP) and 0.3 mg/l of gibberellic acid (GA<sub>3</sub>), while rooting by 1.0 mg/l of indolebutyric acid (IBA). Sodium chloride (NaCl) was added to induce salt stress and its concentration was gradually increased from 0.1% (w/v) onwards. The salt tolerance was observed up to 0.4% (w/v) NaCl and 100% mortality of explants was noted above this concentration. The inclusion of Arbuscular Mycorrhizal (AM) fungi and Azotobacter chroococcum to tissue culture of raised saplings during acclimatization enhanced their survival and resulted in a significant increase of plant growth. After the transfer of plants to salt affected wasteland, only NaCl-treated saplings survived, whereas those developed without NaCl resulted in 100% mortality. &nbsp;

EFFECTS OF BAP CONCENTRATION ON IN VITRO SHOOT MULTIPLICATION AND CALLUS INDUCTION OF JATROPHA CURCAS

2011 ◽  
pp. 203-208 ◽  
Author(s):  
A. Shamsiah ◽  
A. Awal ◽  
S. Nurathrah ◽  
M. Khairul-Azmir ◽  
J.S. Norrizah
Keyword(s):  
Callus Induction ◽  
Jatropha Curcas ◽  
Shoot Multiplication

scholarly journals In vitro propagation of Codonopsis javania (Blume) Hook. f. et Thomson through the callus induction

2019 ◽  
Vol 2 (4) ◽  
pp. 56-61 ◽  
Author(s):  
Vi Thi Tuong Nguyen ◽  
Trinh Le Diem Ho ◽  
Kim Thi A Phan
Keyword(s):  
Callus Induction ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Induction Medium ◽  
Ms Medium ◽  
Root Yield ◽  
Yield Quality ◽  
Shoot Induction Medium

Codonopsis javania (Blume) Hook.f. et Thomson a traditional medicine plant and now an endangered species in Vietnam is grown for roots. The research was carried out to establish the plant propagation for the purpose of concerving and exploting this endangered medicinal herbs. In vitro shoot tip explants (1 – 1.5 cm) were induced to form callus on MS medium containing NAA (0.5 – 2 mg /L) with TDZ 0.1 mg/L. After four weeks of culture, in the MS medium combine with NAA 1 mg/L and TDZ 0.1 mg/L the explant induced compact callus (green, solid) wsa achieved 85.33%. The callus induction to form shoots on medium MS containing BA (0.5 – 2.0 mg/L) with NAA 0.2 mg/L. After 4 weeks of culture, shoot formation was higher in the MS medium containing BA 1.0 mg /L and NAA 0.2 mg/L and achieved of 82.67 % with 9.92 shoots/explant. The best shoot proliferation (2 – 3 cm) was excised and transferred to a medium shoot multiplication with the same composition as the shoot induction medium in which NAA 0.2 mg/L was replaced by NAA 0.5 mg/L. When compared the shoot multiplication between the two mediums at the same BA concentration (2 mg/L), all shoots increased and reached 5.87 times after 60 days cultured. On rooting MS medium with IBA 1 mg/L, 88.67 % in vitro rooting was observed with the average root yield of 4.33 roots/shoot and the length of 8.27 cm. Root length and their yield quality were highly improved when using of coconut fiber (30 %) and earthworms compost (70 %) (v/v) in the transfer medium after acclimatisation stages.

scholarly journals Micropropagation Of Merremia Quinquefolia (L.) Hallier F. From Nodal Explants

2015 ◽  
Vol 23 (1) ◽  
pp. 13-16
Author(s):  
Mafatlal M. Kher ◽  
M. Nataraj ◽  
Hettal D. Parmar ◽  
Hasmatbanu Buchad
Keyword(s):  
Shoot Multiplication ◽  
Nodal Explants ◽  
Ms Medium ◽  
Mineral Salts ◽  
Single Node ◽  
The Family ◽  
Sedative Effects ◽  
Bud Breaking ◽  
Important Medicinal Plant

AbstractMerremia quinquefolia, is an important medicinal plant of the family Convolvulaceae known for its vasoconstrictor, uterotonic, neurohormonic, sympathicolytic and sedative effects. In the present investigation effect of cytokinins 6-benzylaminopurine (BAP), kinetin (Kn) and thidiazuron (TDZ), at concentrations 1.0, 2.0, 3.0, 4.0 and 5.0 mg·dm−3 on in vitro shoot multiplication from nodal explants of M. quinquefolia was evaluated. Bud breaking and emergence of shoots started within 10-15 days of inoculation in all media containing cytokinin. Murashige and Skoog (MS) medium supplemented with 4.0 mg·dm−3 BAP resulted in maximum number of shoots from single node within 45 days. In vitro raised shoots were successfully rooted on ½ mineral salts of MS medium with 3% sucrose supplemented with 2.0 mg·dm−3 indole-3-butyric acid (IBA). This is the first report on in vitro propagation of Merremia quinquefolia. This study can be useful for development of micropropagation protocols for related taxa.

Improved shoot multiplication and development in hybrid hazelnut nodal cultures by ethylenediamine di-2-hydroxy-phenylacetic acid (Fe-EDDHA)

2013 ◽  
Vol 93 (3) ◽  
pp. 511-521 ◽  
Author(s):  
Walter Garrison ◽  
Adam Dale ◽  
Praveen K. Saxena
Keyword(s):  
Culture Medium ◽  
Phenylacetic Acid ◽  
Shoot Multiplication ◽  
Distal Region ◽  
Nodal Explants ◽  
Tetraacetic Acid ◽  
Double Phase ◽  
Phase Culture ◽  
Forms Of Iron

Garrison, W., Dale, A. and Saxena, P. K. 2013. Improved shoot multiplication and development in hybrid hazelnut nodal cultures by ethylenediamine di-2-hydroxy-phenylacetic acid (Fe-EDDHA). Can. J. Plant Sci. 93: 511–521. Micropropagation of hybrid hazelnut cultivars is difficult because of their recalcitrant nature. The current study assessed the effect of different iron sources on in vitro shoot multiplication and subsequent plantlet development from nodal explants of the cultivar Geneva. Two chelated forms of iron, ethylenediamine di-2-hydroxy-phenylacetic acid (Fe-EDDHA) and ethylenediamine tetraacetic acid (Fe-EDTA) were tested to determine the effect on shoot development. Shoots were longer and had a higher number of nodes when cultured on a modified NCGR-COR medium supplemented with 230 µM Fe-EDDHA, whereas shoots failed to grow on a medium with 460 or 690 µM Fe-EDTA. All plantlets grown in the presence of Fe-EDDHA had more chlorophyll, larger leaves, and higher dry weights compared with Fe-EDTA. Electron microscopy of in vitro grown tissues revealed that the form of Fe influenced the number of granal and stromal lamellae per chloroplast, the number of thylakoids per granum, and the overall chloroplast structure. Nodal explants originating from the proximal end of stems developed longer shoots with more nodes than those derived from the distal region. The use of double-phase culture medium produced plants with longer shoots and more nodes, although these exhibited hyperhydricity, showed greater morphological variation, and contained less chlorophyll. These results demonstrate the efficacy of the use of Fe-EDDHA in growth medium for improving micropropagation efficiency of hazelnut.

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