scholarly journals Shoot Multiplication and Callus Induction of Labisia pumila var. alata as Influenced by Different Plant Growth Regulators Treatments and Its Polyphenolic Activities Compared with the Wild Plant

Molecules ◽  
2021 ◽  
Vol 26 (11) ◽  
pp. 3229
Author(s):  
Mat Yunus Najhah ◽  
Hawa Z. E. Jaafar ◽  
Jaafar Juju Nakasha ◽  
Mansor Hakiman
Keyword(s):  
Callus Induction ◽  
Antioxidant Activities ◽  
Shoot Multiplication ◽  
Wild Plants ◽  
Wild Plant ◽  
Total Phenolic ◽  
Nodal Segment ◽  
Ms Medium ◽  
In Vitro Plantlets

This study aims to investigate whether the in vitro-cultured L. pumila var. alata has higher antioxidant activity than its wild plant. An 8-week-old L. pumila var. alata nodal segment and leaf explants were cultured onto Murashige and Skoog (MS) medium supplemented with various cytokinins (zeatin, kinetin, and 6-benzylaminopurine (BAP)) for shoot multiplication and auxins (2,4-dichlorophenoxyacetic acid (2,4-D) and picloram) for callus induction, respectively. The results showed that 2 mg/L zeatin produced the optimal results for shoot and leaf development, and 0.5 mg/L 2,4-D produced the highest callus induction results (60%). After this, 0.5 mg/L 2,4-D was combined with 0.25 mg/L cytokinins and supplemented to the MS medium. The optimal results for callus induction (100%) with yellowish to greenish and compact texture were obtained using 0.5 mg/L 2,4-D combined with 0.25 mg/L zeatin. Leaves obtained from in vitro plantlets and wild plants as well as callus were extracted and analyzed for their antioxidant activities (DPPH and FRAP methods) and polyphenolic properties (total flavonoid and total phenolic content). When compared with leaf extracts of in vitro plantlets and wild plants of L. pumila var. alata, the callus extract displayed significantly higher antioxidant activities and total phenolic and flavonoid content. Hence, callus culture potentially can be adapted for antioxidant and polyphenolic production to satisfy pharmaceutical and nutraceutical needs while conserving wild L. pumila var. alata.

scholarly journals Bioactive Compounds in Wild, In vitro Obtained, Ex vitro Adapted, and Acclimated Plants of Centaurea davidovii (Asteraceae)

2015 ◽  
Vol 10 (6) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Antoaneta Trendafilova ◽  
Milka Jadranin ◽  
Rossen Gorgorov ◽  
Marina Stanilova
Keyword(s):  
Secondary Metabolites ◽  
Wild Plants ◽  
Total Phenolic ◽  
Ms Medium ◽  
Ex Vitro ◽  
Field Plot ◽  
Bioactive Substances ◽  
Mass Flowering ◽  
Rare And Endangered

In vitro cultures were initiated from a single seed of Centaurea davidovii. Whole plantlets were regenerated and cultivated for several months on agar-solidified nutrient media differing by their composition: basal MS medium, MS medium supplemented with plant growth regulators, and liquid MS medium. Plantlets were ex vitro adapted and successfully acclimated to open-air conditions; flowering was observed in some individuals in the first summer, and mass flowering during the second summer. The contents of the total flavonoids and the total phenolic compounds were determined spectrophotometrically in the leaves of the in vitro plantlets cultured on different media, and then compared with those in the leaves of the wild plants and in the leaves of the acclimated plants of the field plot. The sesquiterpene lactone 8α-(5′-hydroxyangeloyl)-salonitenolide was determined by HPLC in leaf samples of C. davidovii wild plants, in vitro obtained plantlets and ex vitro acclimated plants in the greenhouse and on the experimental field plot. The composition of the nutrient medium influenced the contents of all studied bioactive substances. The highest concentrations of all tested secondary metabolites were detected in the leaves of the acclimated plants during mass flowering, the content of the lactone reaching 56.2 mg/g DW, which was several times more than in the other leaf samples. The obtained results revealed both the effectiveness of biotechnological methods for propagation and conservation of rare and endangered plant species, and the possibility to use C. davidovii plants ex vitro acclimated to field conditions as a source of secondary metabolites with potential biological activity.

scholarly journals In vitro study of Tinospora cordifolia (Willd.) Miers (Menispermaceae)

2010 ◽  
Vol 6 ◽  
pp. 103-105 ◽  
Author(s):  
Aditi Singh ◽  
Saroj K Sah ◽  
Aunji Pradhan ◽  
Sabari Rajbahak ◽  
Niran Maharajan
Keyword(s):  
Medicinal Plant ◽  
Callus Induction ◽  
Shoot Growth ◽  
Tinospora Cordifolia ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium

In vitro study was carried out in an important medicinal plant Tinospora cordifolia (Willd.) Miers belonging to the family: Menispermaceae. Vegetative parts such as stem, leaf and nodal explants were excised from an elite in vivo grown mature plant and thereafter cultured on Murashige-Skoog (MS) medium supplemented with different hormonal concentrations for callus induction and organogenesis. Callus formation occurred from nodal segments, leaf and inter-node explants when planted on different combinations of hormones. Tinospora cordifolia showed response for in vitro shoot growth from the nodal segment. The best shoot growth was observed on MS medium supplemented with kinetin (1.5 mg/l). Similarly, the best result for root induction was obtained on MS medium supplemented with 6-benzylaminopurine (1.0 mg/l) and naphthaleneacetic acid (2.5 mg/l). Key-words: callus induction; explants; medicinal plant; MS medium; tissue culture.DOI: 10.3126/botor.v6i0.2918 Botanica Orientalis - Journal of Plant Science (2009) 6: 103-105

scholarly journals Plant Regeneration Through Nodal Explant Derived Callus in Wood Apple (Aegle marmelos L.)

1970 ◽  
Vol 44 (4) ◽  
pp. 415-420 ◽  
Author(s):  
Ranjoy Das ◽  
M Faruk Hasan ◽  
Harunar Rashid ◽  
Motiur Rahman
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Ms Medium ◽  
Nodal Explant ◽  
Aegle Marmelos ◽  
Half Strength ◽  
Subsequent Regeneration

This study reports on an improved protocol for callus induction and subsequent regeneration from nodal segment of wood apple (Aegle marmelos L.) Creamish friable competent callus was achieved from nodal segments on MS medium augmented with 4.0 mg1-1 2,4-D within two weeks of inoculation. The callus produced large number of shoots when cultured on MS medium fortified with 2.0 mgl-1 BAP+0.1 mgl-1 NAA within ten days of culture. In vitro raised shoots were rooted on half strength MS medium enriched with 1.0 mgl-1 IBA within fifteen days of culture. The rooted plantlets were successfully established with 80% survival. Key words: Plant regeneration; Callus induction; Nodal explant; Aegle marmelos. DOI: 10.3329/bjsir.v44i4.4590 Bangladesh J. Sci. Ind. Res. 44(4), 415-420, 2009

Micropropagation and Evaluation of Phenolic Content, Antioxidant and Antimicrobial Activities of Vernonia cinerea (Linn.) Less

2015 ◽  
Vol 19 ◽  
pp. 36-41
Author(s):  
MA Sonibare ◽  
TO Aremu ◽  
PA Okorie
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Antimicrobial Activities ◽  
Wild Plant ◽  
Total Phenolic ◽  
Ms Medium ◽  
Phenolic Contents ◽  
Total Phenolic Contents ◽  
Antioxidant Agents ◽  
Vernonia Cinerea

Vernonia cinerea, belonging to the family Asteraceae, is of wide medicinal application. This study investigated the antimicrobial, antioxidant activities and the total phenolic contents of wild plant of Vernonia cinerea with its respective shoot cultures.Nodal explants of V. cinerea were cultured on Murashige and Skoog (MS) medium supplemented with various combinations of BAP (0.5-2.5 mg/L) and NAA (0.1-0.5 mg/L) including various concentrations of Gibberllic acid for subculturing. The antimicrobial activity of plant extracts was tested using agar well diffusion and macrodilution methods for zone of inhibition (ZI), minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC).The free radical scavenging test was conducted using 2,2, diphenyl-1-picryl hydrazyl (DPPH) reagent while total phenolic contents (TPC) were measured by Folin–Ciocalteau reagent. Maximum shoot proliferation of 11.42 ± 2.26 cm was obtained from MS medium supplemented with 0.5 mg/L BAP + 0.1 mg/L NAA. All extracts displayed moderate antimicrobial potential against the tested pathogens in the range of 9.0-13.1 mm ZI, with highest MIC of 0.78 mg/mL and 1.56 mg/mL fromMPE and WPE, respectively.The IC50 values of 4.49 and 5.10 μg/mL was obtained in WPE and MPE. The MPE had TPC of 6.66 ± 0.83 mg GAE/g compared to the WPE with TPC of 5.43 ± 1.31 mg GAE/g. The crude methanol extracts of wild and micropropagated plants of V. Cinerea showed high amounts of phenolic compounds, which could present them as candidates for future search for antimicrobial and antioxidant agents for different ailments.Keywords: Micropropagation, Antioxidant, DPPH, Phenolic Compound, Vernonia cinerea

scholarly journals In vitro propagation of Codonopsis javania (Blume) Hook. f. et Thomson through the callus induction

2019 ◽  
Vol 2 (4) ◽  
pp. 56-61 ◽  
Author(s):  
Vi Thi Tuong Nguyen ◽  
Trinh Le Diem Ho ◽  
Kim Thi A Phan
Keyword(s):  
Callus Induction ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Shoot Formation ◽  
Induction Medium ◽  
Ms Medium ◽  
Root Yield ◽  
Yield Quality ◽  
Shoot Induction Medium

Codonopsis javania (Blume) Hook.f. et Thomson a traditional medicine plant and now an endangered species in Vietnam is grown for roots. The research was carried out to establish the plant propagation for the purpose of concerving and exploting this endangered medicinal herbs. In vitro shoot tip explants (1 – 1.5 cm) were induced to form callus on MS medium containing NAA (0.5 – 2 mg /L) with TDZ 0.1 mg/L. After four weeks of culture, in the MS medium combine with NAA 1 mg/L and TDZ 0.1 mg/L the explant induced compact callus (green, solid) wsa achieved 85.33%. The callus induction to form shoots on medium MS containing BA (0.5 – 2.0 mg/L) with NAA 0.2 mg/L. After 4 weeks of culture, shoot formation was higher in the MS medium containing BA 1.0 mg /L and NAA 0.2 mg/L and achieved of 82.67 % with 9.92 shoots/explant. The best shoot proliferation (2 – 3 cm) was excised and transferred to a medium shoot multiplication with the same composition as the shoot induction medium in which NAA 0.2 mg/L was replaced by NAA 0.5 mg/L. When compared the shoot multiplication between the two mediums at the same BA concentration (2 mg/L), all shoots increased and reached 5.87 times after 60 days cultured. On rooting MS medium with IBA 1 mg/L, 88.67 % in vitro rooting was observed with the average root yield of 4.33 roots/shoot and the length of 8.27 cm. Root length and their yield quality were highly improved when using of coconut fiber (30 %) and earthworms compost (70 %) (v/v) in the transfer medium after acclimatisation stages.

scholarly journals Phytochemicals and antioxidant capacity of wild growing and in vitro Hypericum heterophyllum

2020 ◽  
Vol 25 (6) ◽  
pp. 2111-2117
Author(s):  
CENNET YAMAN ◽  
Keyword(s):  
Secondary Metabolites ◽  
Antioxidant Activities ◽  
Principal Component ◽  
Hierarchical Cluster ◽  
Quinic Acid ◽  
Total Phenolic ◽  
Food Preservatives ◽  
Plant Parts ◽  
In Vitro Plantlets

In vitro cultures of Hypericum heterophyllum Vent. was established by using MS and MS-B5 medium contained plant growth regulators such as kinetin, BAP, IAA, TDZ and picloram. Flower, leaf, stem and in vitro samples (callus and in vitro plantlets) of H. heterophyllum were analysed by LC-MS/MS for their chemical contents such as quinic acid, gallic acid, (+)-catechin, ferulic acid, vanillic acid, p-coumaric acid, caffeic acid, and quercetin; moreover their radical scavening activities conducted by DPPH and ABTS methods were evaluated. Among all the analysed samples, the in vitro plantlets shown the highest antioxidant activity (IC50, 220 μg/mL for DPPH and 254 μg/mL for ABTS), probably due to the presence of phenolic acids and flavonoids, specifically the higher total phenolic content (64.4 mg GAE/g extract) than other samples. The phytochemical variation among all samples was discussed through principal component analysis (PCA) and hierarchical cluster analysis (HCA). The in vitro plantlets might offer possibilities for the production of high-value secondary metabolites as pharmaceuticals and food preservatives. This study is the first report on analyses and comparison of secondary metabolites and antioxidant activities in different plant parts and in vitro samples of endemic H. heterophyllum.

scholarly journals In vitro PROPAGATION OF Sprekelia formosissima Herbert., A WILD PLANT WITH ORNAMENTAL POTENTIAL

2010 ◽  
Vol 33 (3) ◽  
pp. 197
Author(s):  
Marisol Cázarez-Prado ◽  
María Andrade-Rodríguez ◽  
Ángel Villegas-Monter ◽  
Irán Alia-Tejacal ◽  
Óscar G. Villegas-Torres ◽  
...  
Keyword(s):  
In Vitro Propagation ◽  
Natural Habitat ◽  
Shoot Multiplication ◽  
Propagation Rate ◽  
Wild Plant ◽  
Ms Medium ◽  
Basal Disc ◽  
Average Multiplication ◽  
Previous Phase

Vegetative propagation of Sprekelia (Sprekelia formosissima Herbert.) in natural conditions is limited because it produces only one bulb per year or none. The objective of this research was to generate an in vitro propagation protocol for this species to increase its commercial propagation rate without extracting the species from its natural habitat. Bulbs of 4 to 5 cm in diameter were used as disinfested donor explant material; 1 cm2 explants were obtained from the cataphyll leaves with and without a portion of basal disc; these explants were established in MS medium supplemented with 8.87 μM of N6 benzyl adenine (BA) and 0.98 μM of indole-3- butyric acid (IBA). For shoot multiplication, bulblets obtained from the previous phase were used as explants and cultivated in MS medium with 2.5, 5, 10, 15 and 20 μM of BA combined with IBA at a 10:1 ratio (BA: IBA). Shoots obtained from multiplication were established in MS medium supplemented with 1, 2, 3, 4, and 5 % (w/v) sucrose to promote growth. Bulblets were rooted in MS medium supplemented with 0, 0.49, 0.98, 1.96, 3.93 and 7.8 μM of IBA. Once roots formed, they were transferred to soil to assess their acclimation. We obtained 89.1 % of aseptic explants, of which 86 % formed two shoots on the average. Multiplication of shoots increased as BA concentration increased in culture medium, and the best results (75 % of bulblets with shoots, 2.66 shoots per bulblet and 2.0 mm diameter shoots) were obtained with 20 μM BA. The best bulb growth in diameter (4.2 mm) and number of bulblet leaves (3.5) was obtained with 5 % sucrose. The use of 0.98 μM IBA resulted in greater rooting percentage (93.7) and number of roots per bulblet (2.0), which were 2.4 cm long on average. Up to 83 % of the bulblets survived acclimation. This protocol to micropropagate Sprekelia formosissima allowed the production of at least 96 bulblets from one mother bulb in a six months period of in vitro culture.

Evaluation of Growth and Antioxidant Activity in Suaeda monoica and Suaeda nudiflora Callus Cultures under Sequential Exposure to Saline Conditions

2019 ◽  
Vol 8 (1) ◽  
pp. 42-52
Author(s):  
Abhishek Joshi ◽  
Bhanupriya Kanthaliya ◽  
Jaya Arora
Keyword(s):  
Antioxidant Activity ◽  
Callus Culture ◽  
Antioxidant Activities ◽  
Callus Cultures ◽  
Total Phenolic ◽  
Specific Cell ◽  
Strong Positive Correlation ◽  
Ms Medium ◽  
Callus Regeneration

Background: Plant in vitro culture systems serve as a useful tool to study the regulatory routes which are related to plant growth and survival under altered environmental conditions. Methods: Callus culture of Suaeda monoica and Suaeda nudiflora were established for studying the salt tolerance mechanism at the cellular level. Calli of both the species were induced from seedling’s epicotyls on Murashige and Skoog (MS) medium supplemented with a different combination of auxin and cytokinins. A sequential stress treatment was given to the callus of both the species. The growth rate of callus, osmolytes and antioxidant activities was investigated after 28 days. A control callus was maintained in each experiment without any salt in the growth medium. Results: Efficient callus regeneration was obtained by exposing the callus tissue to MS medium supplemented with 2,4-dichlorophenoxyacetic acid (2,4-D, 1.0 mg/l), benzylaminopurine (BAP, 0.5 mg/l) and 2,4-D (0.5 mg/l), kinetin (Kn, 0.25 mg/l) for S. monoica and S. nudiflora, respectively. A substantial increase was observed in proline content and a strong positive correlation was found between the total phenolic content and antioxidant activity under increasing salt concentrations. Conclusion: This is the first report on S. monoica callus regeneration. The specific cell lines which were generated through callus culture under sequential saline conditions provide a promising foundation for studying salinity induced expression of enzymes. Further comparison of transcriptomic profiles of control and salt-treated callus cultures can serve as a promising system for the detection of genes responsible for the change in expression under salt stress.

scholarly journals IN VITRO PROPAGATION OF SHOOTS AND CALLUS INDUCTION OF GYMNEMA SYLVESTRE R. BR. “AN IMPORTANT ANTI-DIABETIC PLANT”

2018 ◽  
Vol 10 (3) ◽  
pp. 60 ◽  
Author(s):  
Mohsina Syedy ◽  
Krishnendra Singh Nama
Keyword(s):  
Callus Induction ◽  
Research Work ◽  
Multiple Shoots ◽  
Nodal Segments ◽  
Nodal Segment ◽  
Root Induction ◽  
Ms Medium ◽  
Petiole Explants ◽  
Leaf Petiole

Objective: The objective of this research was to establish and develop a protocol for the mass multiplication and callus induction of an anti-Diabetic plant-G. sylvestre R. Br.Methods: Sterilized explants (Nodal segment and leaf) were used for the initiation of culture. They were cultured on MS medium supplemented with a variety of PGRs (BAP, Kn, IBA, 2,4-D) individually or in combinations.Results: The induction of multiple shoots from nodal segments were highest in MS medium supplemented with 2.0 mg/l Kn and in BAP Maximum shoots were obtained on MS medium fortified with 1 mg/l BAP. For rooting different concentration of IBA were used and highest rooting was recorded on MS medium supplemented with 2.0 mg/l IBA. The rooted Plantlets were hardened initially in culture room conditions and then transferred to mist house. Leaf petiole explants were used for the purpose of callus induction. Best growth was observed in MS medium supplemented with 2,4-D. 1.0 mg/l 2,4-D+0.5 mg/l BAP, 1.0 mg/l 2,4-D+1.5 mg/l Kn.Conclusion: The results obtained in this research work clearly indicated that Kn is a better choice than BAP for the culture initiation. 2 mg/l IBA was proved best for root induction. For callus induction, 1 mg/l 2,4-D gave good results and when callus was sub-cultured on 2,4-D with BAP or Kn then 1.0 mg/l 2, 4-D+1.5 mg/l Kn proved best for mass propagation of callus.

scholarly journals EXPLOITATION OF PLANT EXTRACTS (STEM AND ROOT) OF MICROPROPAGATED BACOPA MONNIERI : ANTIMYCOTIC POTENTIAL

2016 ◽  
Vol 2 (9) ◽  
Author(s):  
MRRIDULA DANGI NARWAL
Keyword(s):  
Antioxidant Activities ◽  
Average Length ◽  
Shoot Multiplication ◽  
Bacopa Monnieri ◽  
Nodal Segments ◽  
Root Induction ◽  
Ms Medium ◽  
In Vitro Production ◽  
Number Of Shoots

Bacopa monnieri is commonly called as brahmi or jal brahmi in India. Brahmi is a non-aromatic herb Brahmi is considered as the herb played a very important role in Ayurvedic medicine. It is found easily India, Australia, Europe, Africa, Asia. Bacopa monnieri (L) belongs to the family Scrophulariaceae is an amphibious plant of tropical and normally found growing on the banks of the rivers and lakes. It is commonly called as brahmi or jal brahmi in India. Brahmi is considered as the main rejuvenating herb played a very important role in Ayurvedic therapies. It also has anti-inflammatory, analgesic, antipyretic, epilepsy, anticancer, antioxidant activities and recently antimycotic preoperty has been reported. The micro propagation protocol of the medicinally important plant Bacopa monnieri was standardized using nodal segments as explants. They were surface sterilized with HgCl2 (0.1%) for 3 minutes prior to inoculation on MS media supplemented with BAP (0.5- 2.5 mg/l); IAA (0.1-0.5 mg/l for shooting,1.0-1.5 mg/l for rooting); NAA (0.1- 0.5 mg/l for shooting, 1.0-1.5 mg/l for rooting). The best performance for shoot multiplication was showed in MS medium supplemented with 1.5 mg/l BAP + 0.5 mg/l IAA. In this combination the number of shoots per explant was 16 and average length of shoot 5.54 ± 0.54 cm. But when different concentrations of NAA were applied along with 1.5 mg/l BAP the number of shoots per explant was 14 and average shoot length was 3.46 ± 0.43 on media. For root induction, best rooting was observed with half strength of MS medium supplemented with IAA (1.0 mg/l). In this combination, it was observed that the number of roots was 12 and average root length of 2.80 ± 0.09. The present study is a stepping stone for in vitro production of required active principles of Bacopa monnieri.

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