scholarly journals Production of Polyclonal Antisera to a Recombinant Coat Protein of Potato virus Y Expressed in Escherichia coli and its Application for Immunodiagnosis

2013 ◽  
Vol 10 (1) ◽  
pp. 1-16
Author(s):  
A.M. Abdel-Sala ◽  
A.K. El-Attar ◽  
C.F. Gambley
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Polyclonal Antisera ◽  
Recombinant Coat Protein

Expression and assembly of the potato virus Y (PVY) coat protein (CP) in Escherichia coli cells

1993 ◽  
Vol 28 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Yehuda Stram ◽  
Ilan Sela ◽  
Orit Edelbaum ◽  
Edna Tanne ◽  
Miri Karchi ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Escherichia Coli Cells

scholarly journals Mapping antigenic epitopes of potato virus Y with antibodies affinity-purified by using overlapping synthetic peptides

1994 ◽  
Vol 3 (2) ◽  
pp. 207-211
Author(s):  
Maija Vihanen-Rantanen ◽  
Reijo Sironen ◽  
Matti Vuento
Keyword(s):  
Amino Acid ◽  
Coat Protein ◽  
Amino Acid Sequence ◽  
Potato Virus ◽  
Synthetic Peptides ◽  
Potato Virus Y ◽  
Detection Methods ◽  
Polyclonal Antisera ◽  
Antigenic Epitopes

Synthetic, overlapping peptides representing the entire amino acid sequence of potato virus Y (PVY) coat protein were used to affinity-purify antibodies from polyclonal antisera to PVY. In testing the binding of the purified antibodies to PVY particles, antigenic epitopes were identified. The N-terminal and C-terminal regions of the PVY coat protein were found to contain most of the antigenic epitopes. The results will facilitate the development of detection methods for PVY based on synthetic peptides.

Expression of Coat Protein of an Ordinary Strain of Potato virus Y in Escherichia coli and Production of Polyclonal Antibodies for Diagnosis

2013 ◽  
Vol 161 (9) ◽  
pp. 671-674
Author(s):  
Arjunan Jeevalatha ◽  
Priyanka Kaundal ◽  
Nitya Nand Sharma ◽  
Priyanka Thakur ◽  
Swarup Kumar Chakrabarti ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Polyclonal Antibodies ◽  
Ordinary Strain

Production of polyclonal antibodies to a recombinant coat protein of potato virus Y

2008 ◽  
Vol 53 (5) ◽  
pp. 438-442 ◽  
Author(s):  
J. Folwarczna ◽  
H. Plchová ◽  
T. Moravec ◽  
H. Hoffmeisterová ◽  
P. Dědič ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Polyclonal Antibodies ◽  
Recombinant Coat Protein

Down Regulation of Potato Virus Y (PVY) Coat Protein (CP) Expression by Iberis gibraltarica Protein Extract

2021 ◽  
Vol 55 (1) ◽  
pp. 80-86
Author(s):  
Muhammad Bilal ◽  
Bushra Tabassum ◽  
Qurban Ali ◽  
Idrees Ahmad Nasir
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Protein Extract ◽  
Down Regulation

scholarly journals Cloning and sequencing of the 3′ half of a potato virus Y (O strain) genome encoding the 5k protein, protease, polymerase and coat protein

1992 ◽  
Vol 20 (13) ◽  
pp. 3515-3515 ◽  
Author(s):  
Makoto Hidaka ◽  
Yukiko Yoshida ◽  
Haruhiko Masaki ◽  
Shigetou Namba ◽  
Shuichi Yamashita ◽  
...  
Keyword(s):  
Coat Protein ◽  
Potato Virus ◽  
Potato Virus Y ◽  
Cloning And Sequencing ◽  
Strain Genome
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