scholarly journals Histopathology of the Hepatopancreas of Pacific White Shrimp, Penaeus vannamei from None Early Mortality Syndrome (EMS) Shrimp Ponds

2015 ◽  
Vol 10 (6) ◽  
pp. 562-568 ◽  
Author(s):  
Hidayah Manan ◽  
Julia Moh Hwei Zhong ◽  
Fariduddin Othman ◽  
Mhd Ikhwanuddi
Author(s):  
Noriaki Akazawa ◽  
Mitsuru Eguchi

Microcosm experiments simulating the occurrence of early mortality syndrome/acute hepatopancreatic necrosis disease (EMS/AHPND) in white shrimp production ponds were performed in 30-L aquariums. Healthy white shrimp, Litopenaeus vannamei, were reared in aquariums containing EMS/AHPND-free hatchery or pond water. Raw pond sludge, collected from shrimp ponds where EMS/AHPND had occurred, was added to some test aquariums, while others were treated with sterilized pond sludge. In some aquariums, water pH was increased from 7.5 to 8.8. Microcosms with stable pH (around 7.5) and/or autoclaved sludge served as controls. The combination of raw sludge and increased pH induced EMS/AHPND and killed white shrimp, whereas raw sludge/stable pH and autoclaved sludge/increased pH combinations did not affect healthy shrimp. Thus, EMS/AHPND outbreaks are due not only to the causative agent but also to environmental stresses such as pH fluctuation. These findings contribute to improved management in shrimp production farms.


2021 ◽  
Author(s):  
Ge Li ◽  
Guosi Xie ◽  
Hailiang Wang ◽  
Xiaoyuan Wan ◽  
Xinshu Li ◽  
...  

Aquaculture ◽  
2021 ◽  
pp. 736905
Author(s):  
Aya S. Hussain ◽  
Deyaaedin A. Mohammad ◽  
Wafaa S. Sallam ◽  
Nahla M. Shoukry ◽  
D. Allen Davis

Author(s):  
Thomas Caceci ◽  
Kay F. Neck ◽  
Donal D H. Lewis ◽  
Raymond F. Sis

Fourteen specimens of the hepatopancreas of the Pacific white shrimp, Penaeus vannamei, were prepared for examination with the transmission and scanning electron microscopes and with the light microscope. The histology and ultrastructure of this organ is similar to that seen in other Decapoda. At the ultrastructural level, it was observed that B-cells rupture at approximately the level of gap junctions located on the lateral plasma membranes of the cells, and discharge the contents of their large vacuoles into the intercellular space. This efflux of enzymatic material may be the mechanism by which cells are released from the wall of the tubule at the proximal end: the rupture and collapse of a B-cell may be analagous to the removal of the keystone which supports an arch. Deprived of support, and lacking structural adaptations for cohesion (there are no desmosomes or interdigitations in the epithelium) and with the intercellular material digested, the remaining intact cells collapse into the lumen of the tubule. The lysis of individual cells of all types - R-, F-, and B-cells - may contribute to the tubules’ total complement of digestive enzymes.


PeerJ ◽  
2018 ◽  
Vol 6 ◽  
pp. e5826 ◽  
Author(s):  
Muhammad Zarul Hanifah Md Zoqratt ◽  
Wilhelm Wei Han Eng ◽  
Binh Thanh Thai ◽  
Christopher M. Austin ◽  
Han Ming Gan

Aquaculture production of the Pacific white shrimp is the largest in the world for crustacean species. Crucial to the sustainable global production of this important seafood species is a fundamental understanding of the shrimp gut microbiota and its relationship to the microbial ecology of shrimp pond. This is especially true, given the recently recognized role of beneficial microbes in promoting shrimp nutrient intake and in conferring resistance against pathogens. Unfortunately, aquaculture-related microbiome studies are scarce in Southeast Asia countries despite the severe impact of early mortality syndrome outbreaks on shrimp production in the region. In this study, we employed the 16S rRNA amplicon (V3–V4 region) sequencing and amplicon sequence variants (ASV) method to investigate the microbial diversity of shrimp guts and pond water samples collected from aquaculture farms located in Malaysia and Vietnam. Substantial differences in the pond microbiota were observed between countries with the presence and absence of several taxa extending to the family level. Microbial diversity of the shrimp gut was found to be generally lower than that of the pond environments with a few ubiquitous genera representing a majority of the shrimp gut microbial diversity such as Vibrio and Photobacterium, indicating host-specific selection of microbial species. Given the high sequence conservation of the 16S rRNA gene, we assessed its veracity at distinguishing Vibrio species based on nucleotide alignment against type strain reference sequences and demonstrated the utility of ASV approach in uncovering a wider diversity of Vibrio species compared to the conventional OTU clustering approach.


2010 ◽  
Vol 41 (4) ◽  
pp. 648-654 ◽  
Author(s):  
H. M. Esparza-Leal ◽  
J. T. Ponce-Palafox ◽  
W. Valenzuela-Quiñónez ◽  
J. L. Arredondo-Figueroa ◽  
Manuel García-Ulloa Gómez

2020 ◽  
pp. 92-118
Author(s):  
David I. Prangnell ◽  
Leandro F. Castro ◽  
Abdulmehdi S. Ali ◽  
Craig L. Browdy ◽  
Paul V. Zimba ◽  
...  

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