Bioconversion of Some Agro-industrial By-products into Single Cell Oil Using Candida albicans NRRL Y-12983 and Lipomyces starkeyi NRRL Y-11557

AbstractSingle cell oil is considered as a promising alternative to fish and vegetable oils. Due to the ability of oleaginous microorganisms to produce significant amounts of polyunsaturated fatty acids such as docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), single cell oil can be used for human and animal consumption. In this context, marine microorganisms of the class Labyrinthulomycetes are particularly highlighted in scientific literature. In order to clarify the possibilities for the use of microorganisms of the class Labyrinthulomycetes in large-scale single cell oil production, the first step is to gain awareness of the cultivation conditions required for these microorganisms. In addition, to reduce the cost of single cell oil production, it is necessary to identify potential agroindustrial waste products that are suitable for microbiological cultivation of Labyrinthulomycetes. This article examines the potential biotechnological applications of Labyrinthulomycetes in single cell oil production, the cultivation conditions, nutritional requirements, inhibitors and suitable waste products. In addition, an agroindustrial by-product availability analysis has been carried out. The article evaluates agroindustrial by-products based on their availability in Latvia, required pre-treatment, price, shelf life and efficiency.

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From paper mill waste to single cell oil: Enzymatic hydrolysis to sugars and their fermentation into microbial oil by the yeast Lipomyces starkeyi

Bioresource Technology ◽

10.1016/j.biortech.2020.123790 ◽

2020 ◽

Vol 315 ◽

pp. 123790 ◽

Cited By ~ 1

Author(s):

Nicola Di Fidio ◽

Federico Dragoni ◽

Claudia Antonetti ◽

Isabella De Bari ◽

Anna Maria Raspolli Galletti ◽

...

Keyword(s):

Enzymatic Hydrolysis ◽

Single Cell ◽

Paper Mill ◽

Microbial Oil ◽

Single Cell Oil ◽

Lipomyces Starkeyi ◽

Paper Mill Waste ◽

Mill Waste

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Single Cell Oil Production from Waste Biomass: Review of Applicable Industrial By-Products

Environmental and Climate Technologies ◽

10.2478/rtuect-2019-0071 ◽

2019 ◽

Vol 23 (2) ◽

pp. 325-337

Author(s):

Kriss Spalvins ◽

Ilze Vamza ◽

Dagnija Blumberga

Keyword(s):

Single Cell ◽

Industrial Waste ◽

Essential Fatty Acids ◽

Production Costs ◽

Industrial Wastes ◽

Biodiesel Production ◽

Waste Materials ◽

Single Cell Oil ◽

Waste Products ◽

By Products

Abstract Single cell oil (SCO) is an attractive alternative source of oil, which, depending on the fatty acid composition, can be used as a feedstock for biodiesel production, as an ingredient for pharmaceuticals or as a source of essential fatty acids for human and animal consumption. However, the use of SCO is limited due to use of relatively expensive food or feed products in the cultivation of SCO producing microorganisms. In order to reduce SCO production costs, the use of cheaper feedstock such as biodegradable agro-industrial wastes are necessary. At the same time, the microbial treatment of biodegradable wastes ensures the neutralization of environmentally harmful compounds and reduces the negative impact on the environment. Oleaginous microorganisms are capable of fermenting a variety of industrial by-products, waste products and wastewaters, however further discussion on properties of the waste materials is necessary to facilitate the selection of the most appropriate waste materials for SCO production. Thus, this review compares various industrial waste products that can be used as cheap feedstock for the cultivation of SCO producing microorganisms. Industrial waste products, by-products and wastewaters are compared according to their global availability, current use in competing industries, required pre-fermentation treatments, oleaginous microorganism cell concentrations and SCO yields.

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Single Cell Oil Production by Wild Type Strain Lipomyces starkeyi Y604

IOP Conference Series Earth and Environmental Science ◽

10.1088/1755-1315/439/1/012002 ◽

2020 ◽

Vol 439 ◽

pp. 012002

Author(s):

E Agustriana ◽

A B Juanssilfero ◽

A Andriani ◽

Fahrurrozi ◽

R Pangestu ◽

...

Keyword(s):

Single Cell ◽

Type Strain ◽

Wild Type Strain ◽

Oil Production ◽

Wild Type ◽

Single Cell Oil ◽

Lipomyces Starkeyi

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Biorefinery-Based Approach to Exploit Mixed Cultures of Lipomyces starkeyi and Chloroidium saccharophilum for Single Cell Oil Production

Energies ◽

10.3390/en14051340 ◽

2021 ◽

Vol 14 (5) ◽

pp. 1340

Author(s):

Gaetano Zuccaro ◽

Angelo del Mondo ◽

Gabriele Pinto ◽

Antonino Pollio ◽

Antonino De Natale

Keyword(s):

Single Cell ◽

Mixed Culture ◽

Synergistic Effects ◽

Low Cost ◽

Food Additives ◽

Fatty Acid Distribution ◽

Arundo Donax ◽

Mixed Cultures ◽

Single Cell Oil ◽

Lipomyces Starkeyi

The mutualistic interactions between the oleaginous yeast Lipomyces starkeyi and the green microalga Chloroidium saccharophilum in mixed cultures were investigated to exploit possible synergistic effects. In fact, microalga could act as an oxygen generator for the yeast, while the yeast could provide carbon dioxide to microalga. The behavior of the two microorganisms alone and in mixed culture was studied in two synthetic media (YEG and BBM + G) before moving on to a real model represented by the hydrolysate of Arundo donax, used as low-cost feedstock, and previously subjected to steam explosion and enzymatic hydrolysis. The overall lipid content and lipid productivity obtained in the mixed culture of YEG, BBM + G and for the hydrolysate of Arundo donax were equal to 0.064, 0.064 and 0.081 glipid·gbiomass−1 and 30.14, 35.56 and 37.22 mglipid·L−1·day−1, respectively. The mixed cultures, in all cases, proved to be the most performing compared to the individual ones. In addition, this study provided new input for the integration of Single Cell Oil (SCO) production with agro-industrial feedstock, and the fatty acid distribution mainly consisting of stearic (C18:0) and oleic acid (C18:1) allows promising applications in biofuels, cosmetics, food additives and other products of industrial interest.

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