Antioxidant and in vitro Toxicity Assessment of Elegant Explosion Daylily Flower (Hemerocallis spp.) Extract in Hepatic Cell Line

2021 ◽  
Vol 16 (1) ◽  
pp. 22-36
Author(s):  
J. Johnson ◽  
S. Ogutu ◽  
J. Boateng
Keyword(s):  
Cell Line ◽  
Toxicity Assessment ◽  
Hepatic Cell ◽  
In Vitro Toxicity ◽  
Hepatic Cell Line ◽  
In Vitro Toxicity Assessment

scholarly journals Bare plasmonic metal nanoparticles: synthesis, characterisation and in vitro toxicity assessment on a liver carcinoma cell line

2020 ◽  
Vol 14 (9) ◽  
pp. 851-857
Author(s):  
Nabojit Das ◽  
Akash Kumar ◽  
Somendu Kumar Roy ◽  
Neeraj Kumar Satija ◽  
Rayavarapu Raja Gopal
Keyword(s):  
Metal Nanoparticles ◽  
Cell Line ◽  
Carcinoma Cell ◽  
Toxicity Assessment ◽  
Carcinoma Cell Line ◽  
In Vitro Toxicity ◽  
Liver Carcinoma ◽  
Nanoparticles Synthesis ◽  
In Vitro Toxicity Assessment

Synthesis, Characterization and In vitro Evaluation of N-Substituted- Sulfomoyl-Phenyl-Amino Carboxylic Acid Derivatives as Squalene Synthase Inhibitors

2019 ◽  
Vol 15 (4) ◽  
pp. 415-426
Author(s):  
Avani B. Chokshi ◽  
Mahesh T. Chhabria ◽  
Pritesh R. Desai
Keyword(s):  
Carboxylic Acid ◽  
Cell Line ◽  
Hepatic Cell ◽  
Squalene Synthase ◽  
Lipid Lowering ◽  
Assay Method ◽  
Aromatic Substituent ◽  
Hepatic Cell Line ◽  
Amino Carboxylic Acid

Background:Squalene Synthase is one of the cholesterol biosynthetic pathway enzymes, inhibition of which produces potent lipid lowering action. A variety of chemical classes have been evaluated for its inhibition to provide alternate antihyperlipidemic agents to statins.Methods:A series of N-substituted-sulfomoyl-phenyl-amino carboxylic acid derivatives were designed through pharmacophore modelling as Squalene Synthase inhibitors. We report here the synthesis, characterization and in vitro pharmacological screening of the designed molecules as Squalene synthase inhibitors. The target molecules were synthesized by a simple procedure and each molecule was characterized by IR, Mass, 1HNMR and 13CNMR spectroscopic techniques. As a primary site of action for cholesterol biosynthesis is liver, each of the molecules were first screened for in vitro cytotoxicity over human hepatic cell line (HepG2) by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay method. The enzyme inhibition assay was performed on cell lysates prepared from HepG2 cells by Human Squalene Synthase ELISA kit, where test compounds were added in the nontoxic concentrations only.Results:Compound 5f was found to be most potent with the IC50 value of 11.91 µM. The CTC50 value for 5f on human hepatic cell line was > 1000 µM so it was considered that the compound was relatively safe and might be free of hepatotoxicity.Conclusion:From the results of our studies, it was observed that compounds with poly nuclear aromatic or hetero aromatic substituent on a side chain were more potent enzyme inhibitors and a distance of 4-5 atoms is optimum between amide nitrogen and hydroxyl group of carboxylic acid.

scholarly journals New insight into artifactual phenomena during in vitro toxicity assessment of engineered nanoparticles: Study of TNF-α adsorption on alumina oxide nanoparticle

2013 ◽  
Vol 27 (3) ◽  
pp. 1049-1056 ◽  
Author(s):  
Mélanie Pailleux ◽  
Delphine Boudard ◽  
Jérémie Pourchez ◽  
Valérie Forest ◽  
Philippe Grosseau ◽  
...  
Keyword(s):  
Toxicity Assessment ◽  
Engineered Nanoparticles ◽  
In Vitro Toxicity ◽  
Tnf Α ◽  
Alumina Oxide ◽  
Oxide Nanoparticle ◽  
In Vitro Toxicity Assessment ◽  
Insight Into

scholarly journals In vitro toxicity assessment of emitted materials collected during the manufacture of water pipe plastic linings

2019 ◽  
Vol 31 (4) ◽  
pp. 131-146 ◽  
Author(s):  
Lisa Kobos ◽  
Seyedeh Mahboobeh Teimouri Sendesi ◽  
Andrew J. Whelton ◽  
Brandon E. Boor ◽  
John A. Howarter ◽  
...  
Keyword(s):  
Toxicity Assessment ◽  
In Vitro Toxicity ◽  
Water Pipe ◽  
In Vitro Toxicity Assessment

scholarly journals In vitro toxicity assessment of chitosan oligosaccharide coated iron oxide nanoparticles

2015 ◽  
Vol 2 ◽  
pp. 27-39 ◽  
Author(s):  
Sudeep Shukla ◽  
Alka Jadaun ◽  
Vikas Arora ◽  
Raj Kumar Sinha ◽  
Neha Biyani ◽  
...  
Keyword(s):  
Iron Oxide ◽  
Iron Oxide Nanoparticles ◽  
Toxicity Assessment ◽  
In Vitro Toxicity ◽  
Oxide Nanoparticles ◽  
Chitosan Oligosaccharide ◽  
In Vitro Toxicity Assessment

In vitro toxicity assessment of silver nanoparticles in Chang liver cells and J-774 macrophages

2010 ◽  
Vol 196 ◽  
pp. S284 ◽  
Author(s):  
L. Garza-Ocañas ◽  
M. Ramirez-Cabrera ◽  
M.T. Zanatta-Calderon ◽  
R. Lujan-Rangel ◽  
D.A. Ferrer ◽  
...  
Keyword(s):  
Silver Nanoparticles ◽  
Toxicity Assessment ◽  
Liver Cells ◽  
In Vitro Toxicity ◽  
In Vitro Toxicity Assessment ◽  
Chang Liver

scholarly journals In vitro toxicity assessment of extracts derived from sol–gel coatings on polycarbonate intended to be used in food contact applications

2016 ◽  
Vol 93 ◽  
pp. 51-57 ◽  
Author(s):  
Isabelle Séverin ◽  
Krystelle Lionti ◽  
Laurence Dahbi ◽  
Catherine Loriot ◽  
Bérangère Toury ◽  
...  
Keyword(s):  
Sol Gel ◽  
Toxicity Assessment ◽  
In Vitro Toxicity ◽  
Food Contact ◽  
In Vitro Toxicity Assessment
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