Antidiabetic Activity and Phytochemical Screening of Extracts from Indonesian Plants by Inhibition of Alpha Amylase, Alpha Glucosidase and Dipeptidyl Peptidase IV

2015 ◽  
Vol 18 (6) ◽  
pp. 279-284 ◽  
Author(s):  
Berna Elya ◽  
Rosita Handayani ◽  
Rani Sauriasari ◽  
Azizahwati . ◽  
Uqie Shabrina Hasyyati ◽  
...  
Keyword(s):  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
Phytochemical Screening ◽  
Alpha Glucosidase

scholarly journals Assessment of In Vitro Antidiabetic Potential of Purified Anthocyanin Extract from Floral Petals of Wild Balsam Species

2020 ◽  
Vol 10 (3) ◽  
pp. 31-35
Author(s):  
R ARATHY ◽  
K MURUGAN ◽  
KV DINESH BABU ◽  
GS MANOJ
Keyword(s):  
International Diabetes Federation ◽  
Inhibitory Effect ◽  
Alpha Amylase ◽  
Herbal Remedies ◽  
Antidiabetic Activity ◽  
Public Health Issue ◽  
Impatiens Balsamina ◽  
Alpha Glucosidase

Diabetes is a notorious and growing clinical and public health issue. The International Diabetes Federation assumes that 592 million had diabetes by 2035 and that by 2040 the number will increase to 642 million. Cardiovascular corollary accounts for four million deaths annually attributable to diabetes. Evidence reveals that certain glucose-lowering phytochemicals can improve vascular outcomes with type 2 diabetes, which, together with better understanding of using multiple therapies concurrently, offers opportunities for beneficial personalization of medication regimens. Anthocyanins are coloured pigments and are natural antioxidants. Keeping this in focus, this study was undertaken to evaluate the in vitro antidiabetic activity in the petals of wild Impatiens balsamina L. The anthocyanin was extracted from floral petals of wild balsam species and purified to homogeneity using chromatographic techniques. Evaluation of in vitro antidiabetic properties of anthocyanin extract revealed a dose-dependent increase in the inhibitory effect on the alpha-glucosidase (200 μg/ml) and alpha-amylase enzymes (500 μg/ml) and was comparable with the standard acarbose drug (189 μg/ml and 50 μg/ml). These results indicated that anthocyanin could be used as a source of functional food and nutraceuticals. This information from wild species will be useful in finding more potent antidiabetic principle from the natural resources for the clinical development of antidiabetic therapeutics. Future studies are planned to substantiate the antidiabetic power of anthocyanin using in vivo animal models. Keywords: Alpha amylase, alpha glucosidase, diabetes, herbal remedies, Impatiens balsamina L.

scholarly journals ANTIDIABETIC ACTIVITY, ALPHA-AMYLASE AND ALPHA-GLUCOSIDASE INHIBITORY EFFECT OF PASTINACA SATIVA EXTRACT

2021 ◽  
Vol 0 (0) ◽  
pp. 0-0
Author(s):  
Nagaraju J ◽  
Rajasekhar reddy Alavala ◽  
Subhakar Raju R ◽  
Koteswara Rao GSN ◽  
D S N B K Prasanth ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
Pastinaca Sativa ◽  
Alpha Glucosidase

scholarly journals Phytochemical Screening, Invitro antidiabetic activity of Muntingia calabura leaves extract on alpha-amylase and alpha-glucosidase enzymes

2020 ◽  
Vol 11 (1) ◽  
pp. 1210-1213
Author(s):  
Panneerselvam G ◽  
Jothi Narendiran N ◽  
Vasanth S ◽  
Bupesh G ◽  
Prabhu K ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Screening ◽  
Pharmacological Activities ◽  
Standard Methods ◽  
Inhibition Studies ◽  
Chloroform Methanol ◽  
Alpha Glucosidase ◽  
Muntingia Calabura

Muntingia calabura is a medicinal plant broadly used in conventional therapeutic preparation of many pharmacological activities. In the present study, the Invitro antidiabetic property of Muntingia calabura leaves extracts was analyzed by using standard methods. An in vitro anti-diabetic study was done by inhibition of α-amylase and α - glucosidase enzymes. The phytochemical screening of Muntingia Calabura leaves revealed that the extract is rich in the secondary metabolites such as alkaloids, polyphenols and tannins. The in vitro antidiabetic capability of extracts such as Petroleum ether, Chloroform, Methanol and aqueous through α- amylase enzyme and alpha-glucosidase enzyme inhibition studies. The results of the present study concluded that the methanolic extract of Muntingia calabura exhibits 80% in α-amylase and 60% in alpha-glucosidase activity while compared to acarbose. The phytochemistry study portrays the antidiabetic activity of Muntingia calabura is due to the presence of polyphenols.

scholarly journals Comparative Study on the Antidiabetic Efficacy of Unripe and Ripe Fruit Extracts of Siraitia grosvenorii and the Possible Mechanism of Action

2020 ◽  
pp. 10-18
Author(s):  
Xiangli Xu ◽  
Fenglai Lu ◽  
Ziming Yang ◽  
Xiaojie Yan ◽  
Theophine Akunne ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Mechanism Of Action ◽  
Sweet Taste ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
Ripe Fruit ◽  
Unripe Fruit ◽  
Fruit Extract ◽  
Siraitia Grosvenorii ◽  
Alpha Glucosidase

Aims: The ripe fruit of Siraitia grosvenorii (Swingle) C. Jeffrey (Cucurbitaceae) is used in Traditional Chinese Medicine (TCM) for treatment of diabetes. The ripe fruit is very edible, nutritious and of high sweet taste, while the unripe fruit which is usually very bitter and not edible, has not been widely studied. Therefore, the aim of this study is to evaluate the antidiabetic activity of the unripe fruit of S. grosvenorii and to compare it with that of the ripe fruit as well as to determine their possible mechanism of action. Methodology: The method of streptozotocin-nicotinamide induced diabetic animal model in mice was employed and mice with blood glucose greater than 9.0 mmol/l were selected for treatment with unripe fruit extract (UFE) and ripe fruit extract (RFE) for 24 days. Alpha-glucosidase and alpha amylase enzymes inhibitory assays for determination of possible mechanism of action, were performed. Results: The RFE exhibited better efficacy in antidiabetic activity than the UFE, though the later displayed better potency in activity. The RFE, UFE (300 mg/kg) and glibenclamide exhibited significant (P<0.05) percentage blood glucose reduction (PBGR) of 67.04, 60.69 and 62.88%, respectively, in diabetic mice. Alpha glucosidase assay showed median inhibitory concentration (IC50) value of 7.60 and 6.62 mg/ml for RFE and UFE, respectively, while the alpha amylase inhibitory assay exhibited percentage inhibition of 84.78 and 95.10% for RFE and UFE (25 mg/ml), respectively. Conclusion: The ripe and unripe extracts of S. grosvenorii exhibited antidiabetic activities, and RFE proved a better efficacious activity than UFE, which showed more potent activity. In addition, the antidiabetic activity is likely being mediated through alpha glucosidase and alpha amylase enzymes inhibition.

scholarly journals ANTIDIABETIC ACTIVITY, ALPHA-AMYLASE AND ALPHA- GLUCOSIDASE INHIBITORY EFFECT OF PASTINACA SATIVA EXTRACT

2021 ◽  
Vol 44 (2) ◽  
pp. 387-395
Author(s):  
Nagaraju J ◽  
Rajasekhar A ◽  
Subhakar Raju R ◽  
Koteswara Rao GSN ◽  
Prasanth DSNBK ◽  
...  
Keyword(s):  
Inhibitory Effect ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
Pastinaca Sativa ◽  
Alpha Glucosidase

scholarly journals In Vitro Alpha-Amylase and Alpha-Glucosidase Inhibitory Activity and In Vivo Antidiabetic Activity of Withania frutescens L. Foliar Extract

Molecules ◽  
2021 ◽  
Vol 26 (2) ◽  
pp. 293
Author(s):  
Hamza Mechchate ◽  
Imane Es-safi ◽  
Abdelhadi Louba ◽  
Ali S. Alqahtani ◽  
Fahd A. Nasr ◽  
...  
Keyword(s):  
Local Population ◽  
Fasting Blood Glucose ◽  
Continuous Treatment ◽  
Alpha Amylase ◽  
Antidiabetic Activity ◽  
In Vitro Assays ◽  
Diabetic Mice ◽  
Alpha Glucosidase

Withania frutescens L. is a wild perennial woody plant used by the local population for diverse therapeutic purposes. This work aims to study for the first time the potential inhibitory effect of this plant hydroethanolic extract on α-amylase and α-glucosidase activities using in vitro methods and its antidiabetic and antihyperglycemic activities using alloxan-induced diabetic mice as a model for experimental diabetes. Two doses were selected for the in vivo study (200 and 400 mg/kg) and glibenclamide, a well-known antidiabetic drug (positive control) in a subacute study (28 days) where the antihyperglycemic activity was also assessed over a period of 12 h on diabetic mice. The continuous treatment of diabetic mice with the extract of Withania frutescens for 4 weeks succeeded to slowly manage their high fasting blood glucose levels (after two weeks), while the antihyperglycemic test result revealed that the extract of this plant did not control hyperglycemia in the short term. No toxicity signs or death were noted for the groups treated with the plant extract, and it shows a protective effect on the liver and kidney. The in vitro assays demonstrated that the inhibition of alpha-amylase and alpha-glucosidase might be one of the mechanisms of action exhibited by the extract of this plant to control and prevent postprandial hyperglycemia. This work indicates that W. frutescens have an important long term antidiabetic effect that can be well established to treat diabetes.

Cuparane sesquiterpenes from Laurencia natalensis Kylin as inhibitors of alpha-glucosidase, dipeptidyl peptidase IV and xanthine oxidase

2017 ◽  
Vol 25 ◽  
pp. 178-183 ◽  
Author(s):  
Kannan R.R. Rengasamy ◽  
Lenka Poštová Slavětínská ◽  
Manoj G. Kulkarni ◽  
Wendy A. Stirk ◽  
Johannes Van Staden
Keyword(s):  
Xanthine Oxidase ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Alpha Glucosidase

Identification of novel dipeptidyl peptidase IV and α-glucosidase inhibitory peptides from Tenebrio molitor

2021 ◽  
Author(s):  
Fernando Rivero-Pino ◽  
Antonio Guadix ◽  
Emilia M. Guadix
Keyword(s):  
Enzymatic Hydrolysis ◽  
Tenebrio Molitor ◽  
Dipeptidyl Peptidase ◽  
Dipeptidyl Peptidase Iv ◽  
Antidiabetic Activity ◽  
Inhibitory Peptides ◽  
Dpp Iv ◽  
Glucosidase Inhibitors

Enzymatic hydrolysis improved the antidiabetic activity of Tenebrio molitor protein. Peptides below 2000 Da were the most bioactive. Some novel peptides were identified to be DPP-IV and α-glucosidase inhibitors.

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