scholarly journals Phytochemical Screening, Invitro antidiabetic activity of Muntingia calabura leaves extract on alpha-amylase and alpha-glucosidase enzymes

2020 ◽  
Vol 11 (1) ◽  
pp. 1210-1213
Author(s):  
Panneerselvam G ◽  
Jothi Narendiran N ◽  
Vasanth S ◽  
Bupesh G ◽  
Prabhu K ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Antidiabetic Activity ◽  
Phytochemical Screening ◽  
Pharmacological Activities ◽  
Standard Methods ◽  
Inhibition Studies ◽  
Chloroform Methanol ◽  
Alpha Glucosidase ◽  
Muntingia Calabura

Muntingia calabura is a medicinal plant broadly used in conventional therapeutic preparation of many pharmacological activities. In the present study, the Invitro antidiabetic property of Muntingia calabura leaves extracts was analyzed by using standard methods. An in vitro anti-diabetic study was done by inhibition of α-amylase and α - glucosidase enzymes. The phytochemical screening of Muntingia Calabura leaves revealed that the extract is rich in the secondary metabolites such as alkaloids, polyphenols and tannins. The in vitro antidiabetic capability of extracts such as Petroleum ether, Chloroform, Methanol and aqueous through α- amylase enzyme and alpha-glucosidase enzyme inhibition studies. The results of the present study concluded that the methanolic extract of Muntingia calabura exhibits 80% in α-amylase and 60% in alpha-glucosidase activity while compared to acarbose. The phytochemistry study portrays the antidiabetic activity of Muntingia calabura is due to the presence of polyphenols.

Antidiabetic Activity of Aqueous and Methanolic Extract of Dried Pericarp (Shell) of Juglans Regia L.

2019 ◽  
Vol 12 (3) ◽  
pp. 4531-4533
Author(s):  
Krishn Kumar Agrawal ◽  
Nitin Agrawal ◽  
Jyoti Gupta ◽  
Tripanshu Gupta
Keyword(s):  
Aqueous Extract ◽  
Cardiac Glycosides ◽  
Methanolic Extract ◽  
Juglans Regia ◽  
Reducing Sugar ◽  
Antidiabetic Activity ◽  
Assay Method ◽  
Inhibition Assay ◽  
Phytochemical Screening

The aim of the present study was to investigate the in-vitro anti-diabetic activity of aqueous and methanolic extract of dried pericarp (Shell) of Juglans regia L. The preliminary qualitative phytochemical screening was also undertaken to reveal the presence of Phyto-constituents in the extracts. Phytochemical screening of aqueous extract shows the presence of polyphenols, flavanoids, cardiac glycosides, carbohydrates and reducing sugar while methanolic extract show the presence of alkaloids, polyphenols, cardiac glycosides, carbohydrates and reducing sugar. Pharmacological evaluation of Juglans regia was performed by α-amylase inhibition assay method. Percentage inhibition of α amylase at dose of 50mg/mL, 100 mg/mL and 150 mg/mL of aqueous and methanolic extract was found to be 33.33, 66.67, 93.34 and 32.27, 59.64, 90.74 respectively. Among the extracts the aqueous extract showed highest activity as compared to control and methanolic extract.

scholarly journals Antidiabetic activity, alpha-amylase, and alpha-glucosidase inhibitory effect of Tradescantia spathacea Swartz extract

2020 ◽  
Vol 11 (SPL4) ◽  
pp. 1594-1599
Author(s):  
Nagaraju Jesetti ◽  
Rajasekhar Reddy Alavala ◽  
Subhakar Raju Rapaka ◽  
Umasankar Kulandaivelu ◽  
Koteswara Rao GSN
Keyword(s):  
Methanolic Extract ◽  
Amylase Activity ◽  
Inhibitory Effect ◽  
Antidiabetic Activity ◽  
Oral Glucose ◽  
Diabetic Patients ◽  
Diabetic Model ◽  
Alpha Glucosidase

Tradescantia spathacea Swartz belongs to the genus  Commelinaceae, a tropical tree used in many countries as an herbal drug for the care of diabetic patients. The aim of this study was to examine anti-diabetic activity of the Tradescantia spathacea Swartz methanolic extract (METSW) and the in-vitro activity of α-amylase, and α-glucosidase was carried out.  METSW compared with acarbose inhibition of the α-amylase and α-glucosidase enzyme, METSW exhibited IC50 less than 100μg/mL would be considered as healthy. The METSW showed IC50 66.22 ± 0.52μg/mL α-amylase activity, acarbose revealed an IC50 of 83.25 ± 1.28μg/mL. METSW demonstrated IC50 levels of  85.37 ± 0.72 μg/mL (y= 0.095x+41.89) inhibition of  the α-Glucosidase enzymes.  METSW at 400 mg/kg greatly decreased the region under the blood glucose level curve in a typical rat test for oral glucose tolerance. The single dose of the extract decreased dramatically from  211 mg/dl to 89.22 mg/dl at 400 mg/kg  METSW in the alloxan induced diabetic model. METSW possesses strong antidiabetic activity in vivo  and in vitro. Besides, the extract has also been shown to have a significant inhibitory activity of α-amylase and α-glucosidase which may lead to its anti-hyperglycemic function when used in diabetic patients. 

scholarly journals EXTRACTION, ISOLATION AND PURIFICATION OF VARIOUS COMPONENTS OF CASSIA UNIFLORA AND IT’S IN VITRO STUDIES

2020 ◽  
Vol 11 (11) ◽  
pp. 246-253
Author(s):  
Sowmya V ◽  
Syed Arbaz Ahmed ◽  
Syed Sameer ◽  
Polireddy P. ◽  
Rajeshwari Dutt K.
Keyword(s):  
Ir Spectroscopy ◽  
Methanolic Extract ◽  
In Vitro Studies ◽  
Fruit Extract ◽  
Pharmacological Activities ◽  
Isolation And Purification ◽  
Lipoxygenase Inhibition ◽  
Anti Oxidant ◽  
Alpha Glucosidase

Cassia species are one among the plants to possess great therapeutical value due to their wide rage of pharmacological activities. Hence, in our present study, we have isolated the extract of Cassia uniflora and isolated some important constituents using various solvents among which methanolic extract was optimal. The isolated compounds were also characterized by mass and IR spectroscopy. The extract was further checked for anti-oxidant as well as anti-diabetic activity. From the results obtained, the leaves and fruit extract were found to have good alpha-glucosidase as well as 5-lipoxygenase inhibition.

Novel Phytochemical Constituents and their Potential to Manage Diabetes

2020 ◽  
Vol 26 ◽  
Author(s):  
Shaik Ibrahim Khalivulla ◽  
Arifullah Mohammed ◽  
Kokkanti Mallikarjuna
Keyword(s):  
Natural Products ◽  
Large Population ◽  
World Health ◽  
In Vivo Studies ◽  
Antidiabetic Activity ◽  
Therapeutic Drug ◽  
Pharmacological Activities ◽  
Chemical Structures

Background: Diabetes is a chronic disease affecting a large population worldwide and stands as one of the major global health challenges to be tackled. According to World Health Organization, about 400 million are having diabetes worldwide and it is the seventh leading cause of deaths in 2016. Plant based natural products had been in use from ancient time as ethnomedicine for the treatment of several diseases including diabetes. As a result of that, there are several reports on plant based natural products displaying antidiabetic activity. In the current review, such antidiabetic potential compounds reported from all plant sources along with their chemical structures are collected, presented and discussed. This kind of reports are essential to pool the available information to one source followed by statistical analysis and screening to check the efficacy of all known compounds in a comparative sense. This kind of analysis can give rise to few numbers of potential compounds from hundreds, whom can further be screened through in vitro and in vivo studies, and human trails leading to the drug development. Methods: Phytochemicals along with their potential antidiabetic property were classified according to their basic chemical skeleton. The chemical structures of all the compounds with antidiabetic activities were elucidated in the present review. In addition to this, the distribution and their other remarkable pharmacological activities of each species is also included. Results: The scrutiny of literature led to identification of 44 plants with antidiabetic compounds (70) and other pharmacological activities. For the sake of information, the distribution of each species in the world is given. Many plant derivatives may exert antidiabetic properties by improving or mimicking the insulin production or action. Different classes of compounds including sulfur compounds (1-4), alkaloids (5-11), phenolic compounds (12-17), tannins (18-23), phenylpropanoids (24-27), xanthanoids (28-31), amino acid (32), stilbenoid (33), benzofuran (34), coumarin (35), flavonoids (36-49) and terpenoids (50-70) were found to be active potential compounds for antidiabetic activity. Of the 70 listed compounds, majorly 17 compounds are from triterpenoids, 13 flavonoids and 7 are from alkaloids. Among all the 44 plant species, maximum number (7) of compounds are reported from Lagerstroemia speciosa followed by Momordica charantia (6) and S. oblonga with 5 compounds. Conclusion: This is the first paper to summarize the established chemical structures of phytochemicals that have been successfully screened for antidiabetic potential and their mechanisms of inhibition. The reported compounds could be considered as potential lead molecules for the treatment of type-2 diabetes. Further, molecular and clinical trials are required to select and establish the therapeutic drug candidates.

scholarly journals Antioxidant and antidiabetic properties of isolated fractions from methanolic extract derived from the whole plant of Cleome viscosa L.

2020 ◽  
Vol 6 (1) ◽  
Author(s):  
Yarrappagaari Suresh ◽  
Gutha Rajasekar ◽  
Thopireddy Lavanya ◽  
Benne Lakshminarsimhulu ◽  
Kesireddy Sathyavelu Reddy ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
Antidiabetic Activity ◽  
Total Phenolic ◽  
Whole Plant ◽  
Glucose Diffusion ◽  
Ft Ir ◽  
Study Support ◽  
Important Medicinal Plant ◽  
Cleome Viscosa

Abstract Background Cleome viscosa is considered as an important medicinal plant extensively used in India, China, Bangladesh, and a few countries in Africa. In the present study, in vitro anti-radical and antidiabetic potential of isolated fractions of methanolic extract of C. viscosa whole plant (MeCV) has been investigated. The identification of polyphenols and their related functional groups in the bioactive fraction was categorized by using HPLC and FT-IR. Results The total phenolic and flavonoid contents of F-D were higher than those of F-A, F-B, and F-C. The F-D exhibited superior antioxidant capacity when compared with the remaining three fractions. However, the F-D showed the highest glucose diffusion activity over the 30 min–27 h incubation period and also inhibited both α-glucosidase and α-amylase enzyme activity. HPLC analysis revealed the presence of the two known compounds (protocatechuic acid hexoside, rutin) and six unknown compounds in the F-D. FTIR spectrum confirmed the presence of phenol group. Conclusion The isolated F-D obtained from MeCV displayed superior antioxidant and antidiabetic activity which indicate the presence of polyphenols in the fraction. The data findings of the present study support the traditional uses of the whole plant of C. viscosa as a promising natural source of biological medicines for oxidative stress and diabetes.

PHYTOCHEMICAL SCREENING AND PHARMACOLOGICAL EVALUATION OF AEGLE MARMELOS FRUIT

INDIAN DRUGS ◽  
2020 ◽  
Vol 57 (04) ◽  
pp. 59-64
Author(s):  
Pratibha Thaware ◽  
Pushpa Karale ◽  
Mahesh Karale ◽  
Pranati Chavan
Keyword(s):  
Methanolic Extract ◽  
Radical Scavenging ◽  
Reducing Power ◽  
Pharmacological Action ◽  
Diffusion Method ◽  
Phytochemical Screening ◽  
Aegle Marmelos ◽  
Pharmacological Screening ◽  
Hplc Data

The objective of this study was to evaluate the phytochemical and pharmacological screening of fruit extracts of Aegle marmelos using various in vitro methods. The antioxidant activity of the extracts was evaluated by using DPPH free radical scavenging and reducing power method, while well diffusion method was used for antimicrobial activity. Phytochemical screening indicated the presence of flavonoids, and phenolics were abundantly present in methanolic and aqueous extracts of A. marmelos. Methanolic extract showed significant antioxidant potential, which was however inferior to that of ascorbic acid. The methanolic extract showed maximum zone of inhibition against the S. aureus (18 mm) and it was higher than that of the standard tetracycline. The HPLC data predict that the presence of scopoletin, marmelosin and umbelliferone, which may contribute to their pharmacological action.

scholarly journals IN VITRO CYTOTOXIC ACTIVITY OF ISOLATED COMPOUNDS FROM VIBURNUM PUNCTATUM BUCH-HAM EX D. DON

2016 ◽  
Vol 9 (1) ◽  
pp. 85
Author(s):  
A. Renjith Alex ◽  
K. Ilango
Keyword(s):  
Cytotoxic Activity ◽  
Anticancer Activity ◽  
Cell Lines ◽  
Methanol Extract ◽  
Methanolic Extract ◽  
Human Colon ◽  
Human Colon Cancer ◽  
Chloroform Methanol ◽  
Human Colon Cancer Cells

Objective: The main aim of the study was to screen the isolated compounds of Viburnum Punctatum for its in vitro anticancer activity and its percentage viability against HCT 15 (Human Colon Cancer Cells) Cell lines.Methods: Pet ether, Chloroform, Methanol and Aqueous extracts was prepared and assayed for the presence of phytochemicals. Two compounds were isolated from the methanol extract of Viburnum Punctatum by column chromatography such as ME1 (Quercetin) and ME2 (Kaemferol-3-glycoside) characterised by UV, IR, MS, 1H NMR and 13C NMR. The above isolated compounds were subjected to in vitro anticancer activity on HCT 15 cell lines was evaluated by Micro culture Tetrazolium (MTT) assay.Results: ME1 showed significant cytotoxic activity than the ME2 on HCT 15 cells with a percentage viability of 54.60 and 67.18 in the concentration of 10µg/ml and 50µg/ml respectively.Conclusion: On the basis of obtained results, ME1 and ME2 isolated from a methanolic extract of Viburnum Punctatum represent a new group of cytotoxic against HCT 15 Cell lines.

scholarly journals Demonstration of Phytochemicals and In-vitro Antioxidant and Anti-inflammatory activity by methanolic extract of Elettaria cardamomum

2020 ◽  
Vol 3 (3) ◽  
pp. 01-04
Author(s):  
Md. Shahidul Islam
Keyword(s):  
Methanolic Extract ◽  
Research Work ◽  
Inflammatory Activity ◽  
Pharmacological Activities ◽  
Elettaria Cardamomum ◽  
Ic50 Values ◽  
Phytochemical Profile ◽  
Anti Inflammatory ◽  
Dpph Scavenging

The existing research study attempts to untie novel avenues for development of the medicinal exercises of Elettaria cardamomum, fashionable known as the “Queen of Spices” and locally recognized as “elaichi”. Its seeds are utilized as abortifacient, acrid, alexiteric, aromatic, sweet, cardiac tonic, cooling, carminative, digestive, diuretic, expectorant, stimulant and also tonic beneficial in the asthma, haemorrhoids, bronchitis, strangury, renal in addition to vesical calculi, anorexia, halitosis, gastropathy dyspepsia as well as burning sensation. The prime goal of this research work is to evaluate antioxidant as well as anti-inflammatory properties of the traditional Bangladeshi medicinal extracts in addition to examine these activities. The aim in present work was to screen the phytochemical profile as well as pharmacological activities of the methanolic extract of this plant’s leaves. To explore pharmacological actions DPPH scavenging test and the HRBC membrane stabilization techniques were done for the antioxidant and also anti-inflammatory test respectively. The pharmacological works revealed that plant extracts might have noteworthy antioxidant effect which is possibly mediated by the inhibition of DPPH free radical which is accountable for oxidation. The IC50 values by the DPPH scavenging test observed for the standard and the leaves were 106.38µg/ml & 594.47µg/ml respectively. There is also moderate anti-inflammatory activity. The IC50 values for anti-inflammatory activity by standard & leaves were 35.04µg/ml and 944.0 µg/ml respectively.

Preliminary Phytochemical screening of Persicaria sagittata: Future prospective for kidney stone

2021 ◽  
pp. 4367-4370
Author(s):  
Ankita Sharma ◽  
Ishita Ishita ◽  
Inder Kumar ◽  
Kritika Verma ◽  
Bhumika Thakur ◽  
...  
Keyword(s):  
Uric Acid ◽  
Medicinal Plant ◽  
Kidney Stone ◽  
Methanolic Extract ◽  
Biologically Active ◽  
Phytochemical Screening ◽  
Secondary Product ◽  
Standard Methods ◽  
Active Constituents ◽  
Uric Acid Stones

Medicinal plants have bioactive compounds, which are used to treatments of various diseases. In the present study, Persicaria sagittata medicinal plant was used for the preliminary phytochemical screening. Methanol and petroleum ether were used as a solvent for obtaining the extraction of the plant. Both the extracts were further study for qualitative phytochemical screening using standard methods. Phytochemical screening shows that methanolic extract possesses the presence of tannins, flavonoids, and vitamin A as tannins and flavonoids are helpful for the uric acid stones. The study reveals that medicinal plant provides a basis of its use in medicine, improve to further drugs in the pharmaceutical area, and contains different biologically active constituents, and the secondary product is valuable of further analysis.

scholarly journals Alpha-Glucosidase and Alpha-Amylase Inhibitory Activities of Novel Abietane Diterpenes from Salvia africana-lutea

Antioxidants ◽  
2019 ◽  
Vol 8 (10) ◽  
pp. 421 ◽  
Author(s):  
Etsassala ◽  
Badmus ◽  
Waryo ◽  
Marnewick ◽  
Cupido ◽  
...  
Keyword(s):  
Methanolic Extract ◽  
High Resolution Mass Spectrometry ◽  
Ultra Violet ◽  
Cape Floristic Region ◽  
The Body ◽  
Alpha Amylase ◽  
Inhibitory Activities ◽  
Abietane Diterpenes ◽  
Alpha Glucosidase

The re-investigation of a methanolic extract of Salvia africana-lutea collected from the Cape Floristic Region, South Africa (SA), afforded four new abietane diterpenes, namely 19-acetoxy-12-methoxycarnosic acid (1), 3β-acetoxy-7α-methoxyrosmanol (2), 19-acetoxy-7α-methoxyrosmanol (3), 19-acetoxy-12-methoxy carnosol (4), and two known named clinopodiolides A (5), and B (6), in addition to four known triterpenes, oleanolic, and ursolic acids (7, 8), 11,12-dehydroursolic acid lactone (9) and β-amyrin (10). The chemical structural elucidation of the isolated compounds was determined on the basis of one and two dimensional nuclear magnetic resonance (1D and 2D NMR), high-resolution mass spectrometry (HRMS), ultra violet (UV), fourier transform infrared (IR), in comparison with literature data. The in vitro bio-evaluation against alpha-glucosidase showed strong inhibitory activities of 8, 10, and 7, with the half inhibitory concentration (IC50) values of 11.3 ± 1.0, 17.1 ± 1.0 and 22.9 ± 2.0 µg/mL, respectively, while 7 demonstrated the strongest in vitro alpha-amylase inhibitory activity among the tested compounds with IC50 of 12.5 ± 0.7 µg/mL. Additionally, some of the compounds showed significant antioxidant capacities. In conclusion, the methanolic extract of S. africana-lutea is a rich source of terpenoids, especially abietane diterpenes, with strong antioxidant and anti-diabetic activities that can be helpful to modulate the redox status of the body and could therefore be an excellent candidate for the prevention of the development of diabetes, a disease where oxidase stress plays an important role.

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