scholarly journals STUDIES ON THE RELATIONSHIP BETWEEN CLINICAL COURSE AND ANTIBODY TITER TO EPSTEIN-BARR VIRUS IN NASOPHARYNGEAL CARCINOMA

1975 ◽  
Vol 78 (12) ◽  
pp. 1237-1243
Author(s):  
YUKIO INUYAMA ◽  
SHIGEJI SAITO ◽  
RAISUKE OZU ◽  
MASATOSHI HORIUCHI ◽  
KAZUYUKI ASAOKA ◽  
...  
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Antibody Titer ◽  
Epstein Barr Virus ◽  
Clinical Course ◽  
Barr Virus ◽  
Epstein Barr ◽  
The Relationship

The Clinical Significance of IGF-1R and Relationship with Epstein–Barr Virus Markers: LMP1 and EBERs in Tunisian Patients with Nasopharyngeal Carcinoma

2020 ◽  
Vol 129 (10) ◽  
pp. 1011-1019
Author(s):  
Nehla Mokni Baizig ◽  
Ben Ayoub Wided ◽  
Olfa El Amine ◽  
Said Gritli ◽  
Michele ElMay
Keyword(s):  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Survival Rates ◽  
Growth Factor Receptor ◽  
Disease Free Survival ◽  
Immunohistochemical Method ◽  
Barr Virus ◽  
Lmp1 Expression ◽  
Epstein Barr ◽  
The Relationship

Objectives: Tunisia is in the endemic area of nasopharyngeal carcinoma. Epstein–Barr virus (EBV) based assays have been commonly used as standard markers for screening and monitoring the disease. So, it is very important to find novel factors for the early diagnostic and prognostic evaluation of this cancer. The aim of the study was to evaluate the expression of IGF-1R (Insulin Growth Factor Receptor 1), LMP 1 (Latent Membrane Protein 1) and EBERs (EBV encoded RNAs) in order to determine their correlation with clinicopathologic parameters and survival rates in patients with nasopharyngeal carcinoma (NPC). We also looked for the relationship between these biomarkers. Methods: IGF-1R and LMP1 expression was performed by means of immunohistochemical method and EBERs were detected using in situ hybridization of paraffin embedded tumor tissues of 94 patients with nasopharyngeal carcinoma and 45 non-cancerous nasopharyngeal mucosa samples. Results: Our findings demonstrated that IGF-1R was over expressed in 47.87% of NPC patients and only in 2.22% of controls. Positive LMP1 expression was detected in 56.38% of NPC patients and all NPC patients were positive for the EBV-encoded RNAs staining. A statistically significant positive correlation was observed between IGF-1R expression and the tumor size ( P < .001). Kaplan–Meier survival curves showed that NPC patients with a strong IGF-1R expression level have shorter median and 5-year Overall Survival than those with weak expression rates (100.15 vs 102.68 months, P = .08). In addition, median and 5-year Disease-Free Survival was significantly lower in the LMP1 positive NPC patients than in the LMP1 negative ones (53.38 vs 93.37 months, P = .03). Moreover, LMP1 expression correlated strongly with IGF-1R expression ( P = .018). The relationship between these two biomarkers could influence patient survival. Conclusion: IGF1-R and LMP1 could be valuable prognostic markers in Tunisian NPC patients.

Adult Respiratory Papillomatosis: Human Papillomavirus Type and Viral Coinfections as Predictors of Prognosis

1995 ◽  
Vol 104 (10) ◽  
pp. 758-762 ◽  
Author(s):  
Anna Maria Pou ◽  
Frank L. Rimell ◽  
Jeanne A. Jordan ◽  
Pierre Barua ◽  
David L. Shoemaker ◽  
...  
Keyword(s):  
Human Papillomavirus ◽  
Epstein Barr Virus ◽  
Clinical Course ◽  
Laryngeal Papillomatosis ◽  
Barr Virus ◽  
Polymerase Chain ◽  
Epstein Barr ◽  
Simplex Virus ◽  
Aggressive Clinical Course ◽  
The Relationship

Pathologic material and the records of 29 patients with laryngeal papillomatosis were reviewed. The relationship between the type of human papillomavirus (HPV) and the presence of viral coinfections was correlated with clinical outcome. Using polymerase chain reaction, paraffin-embedded specimens were analyzed for the presence of HPV, Epstein-Barr virus (EBV), cytomegalovirus (CMV), and herpes simplex virus (HSV). The HPV type could be identified in 24 patients' specimens. Twenty-one patients were infected with HPV type 6. The other 3 were infected with HPV type 11 or 16. Three patients developed squamous cell carcinoma, of whom 2 had HPV type 11 or 16. We found HSV, EBV, and CMV in 50%, 12.5%, and 0% of specimens, respectively. An aggressive clinical course was observed in 17 patients. Evidence of coinfection with other viruses was identified in 11 (65%) of these patients. In contrast, a benign clinical course was observed in 7 patients, of whom 2 (29%) had viral coinfections. We conclude that the HPV type and the presence of viral coinfections may be predictive of an aggressive clinical course.

Studies of Epstein-Barr Virus Antigens Using Immunoperoxidase and Immunofluorescence Techniques

1975 ◽  
Vol 33 ◽  
pp. 342-343
Author(s):  
R. Stephens ◽  
K. Traul ◽  
D. Woolf ◽  
P. Gaudreau
Keyword(s):  
Electron Microscopy ◽  
Nasopharyngeal Carcinoma ◽  
Epstein Barr Virus ◽  
Barr Virus ◽  
If Technique ◽  
Infected Cells ◽  
Virus Antigens ◽  
Epstein Barr ◽  
Virus Capsid Antigen ◽  
Antigen Reactivity

A number of antigens have been found associated with persistent EBV infections of lymphoblastoid cells. Identification and localization of these antigens were principally by immunofluorescence (IF) techniques using sera from patients with nasopharyngeal carcinoma (NPC), Burkitt lymphoma (BL), and infectious mononucleosis (IM). Our study was mainly with three of the EBV related antigens, a) virus capsid antigen (VCA), b) membrane antigen (MA), and c) early antigens (EA) using immunoperoxidase (IP) techniques with electron microscopy (EM) to elucidate the sites of reactivity with EBV and EBV infected cells.Prior to labeling with horseradish peroxidase (HRP), sera from NPC, IM, and BL cases were characterized for various reactivities by the indirect IF technique. Modifications of the direct IP procedure described by Shabo and the indirect IP procedure of Leduc were made to enhance penetration of the cells and preservation of antigen reactivity.

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