Enhanced Lycopene Production by UV-C Irradiation in Radiation-Resistant Deinococcus radiodurans R1

ABSTRACT Genetic engineering of radiation-resistant organisms to recover radionuclides/heavy metals from radioactive wastes is an attractive proposition. We have constructed a Deinococcus radiodurans strain harboring phoN, a gene encoding a nonspecific acid phosphatase, obtained from a local isolate of Salmonella enterica serovar Typhi. The recombinant strain expressed an ∼27-kDa active PhoN protein and efficiently precipitated over 90% of the uranium from a 0.8 mM uranyl nitrate solution in 6 h. The engineered strain retained uranium bioprecipitation ability even after exposure to 6 kGy of 60Co gamma rays. The PhoN-expressing D. radiodurans offers an effective and eco-friendly in situ approach to biorecovery of uranium from dilute nuclear waste.

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Chemical structure elucidation of the carotenoids in the extremely radiation-resistant bacterium Deinococcus radiodurans R1

10.1101/2021.05.26.445707 ◽

2021 ◽

Author(s):

Xiao-ling Zhang ◽

Qiao Yang

Keyword(s):

Structure Elucidation ◽

Chemical Structure ◽

Deinococcus Radiodurans ◽

Chemical Properties ◽

Resistant Bacterium ◽

The Other ◽

Radiation Resistant ◽

Deinococcus Radiodurans R1

The main carotenoid in the extremely radiation-resistant bacterium Deinococcus radiodurans is deinoxanthin. In this paper, based on UV-Vis, MS/MS spectra as well as the chemical properties, the other main two carotenoids in this bacterium were identified as ((all-E)-1-hydroxy-3,4-didehydro-1,2-dihydro-β,ψ-carotene-4-one-1), and ((all-E)-1-hydroxy-3,4-didehydro-1,2,2,3-quahydro-β,ψ-carotene-4-one-1), respectively. The fully structure elucidation for the carotenoids should help to gain a better understanding of the radiation-resistant mechanisms in this remarkable bacterium.

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Efficient bioremediation of radioactive iodine using biogenic gold nanomaterial-containing radiation-resistant bacterium, Deinococcus radiodurans R1

Chemical Communications ◽

10.1039/c7cc00720e ◽

2017 ◽

Vol 53 (28) ◽

pp. 3937-3940 ◽

Cited By ~ 17

Author(s):

Mi Hee Choi ◽

Sun-Wook Jeong ◽

Ha Eun Shim ◽

Seong-Jae Yun ◽

Sajid Mushtaq ◽

...

Keyword(s):

Radioactive Iodine ◽

Deinococcus Radiodurans ◽

Resistant Bacterium ◽

Radiation Resistant ◽

Gold Nanomaterial ◽

Deinococcus Radiodurans R1

A new bioremediation method is developed by using a gold nanomaterial-containing radiation-resistant bacterium.

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Coexistence of SOS-Dependent and SOS-Independent Regulation of DNA Repair Genes in Radiation-Resistant Deinococcus Bacteria

Cells ◽

10.3390/cells10040924 ◽

2021 ◽

Vol 10 (4) ◽

pp. 924

Author(s):

Laurence Blanchard ◽

Arjan de Groot

Keyword(s):

Dna Repair ◽

Deinococcus Radiodurans ◽

Dna Repair Genes ◽

Lesion Bypass ◽

Radiation Resistant ◽

Radiation Induced ◽

Induced Mutagenesis ◽

High Doses ◽

Translesion Polymerases ◽

Repair Genes

Deinococcus bacteria are extremely resistant to radiation and able to repair a shattered genome in an essentially error-free manner after exposure to high doses of radiation or prolonged desiccation. An efficient, SOS-independent response mechanism to induce various DNA repair genes such as recA is essential for radiation resistance. This pathway, called radiation/desiccation response, is controlled by metallopeptidase IrrE and repressor DdrO that are highly conserved in Deinococcus. Among various Deinococcus species, Deinococcus radiodurans has been studied most extensively. Its genome encodes classical DNA repair proteins for error-free repair but no error-prone translesion DNA polymerases, which may suggest that absence of mutagenic lesion bypass is crucial for error-free repair of massive DNA damage. However, many other radiation-resistant Deinococcus species do possess translesion polymerases, and radiation-induced mutagenesis has been demonstrated. At least dozens of Deinococcus species contain a mutagenesis cassette, and some even two cassettes, encoding error-prone translesion polymerase DnaE2 and two other proteins, ImuY and ImuB-C, that are probable accessory factors required for DnaE2 activity. Expression of this mutagenesis cassette is under control of the SOS regulators RecA and LexA. In this paper, we review both the RecA/LexA-controlled mutagenesis and the IrrE/DdrO-controlled radiation/desiccation response in Deinococcus.

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Silver Nanomaterial-Immobilized Desalination Systems for Efficient Removal of Radioactive Iodine Species in Water

Nanomaterials ◽

10.3390/nano8090660 ◽

2018 ◽

Vol 8 (9) ◽

pp. 660 ◽

Cited By ~ 9

Author(s):

Ha Shim ◽

Jung Yang ◽

Sun-Wook Jeong ◽

Chang Lee ◽

Lee Song ◽

...

Keyword(s):

Silver Nanoparticles ◽

Radioactive Iodine ◽

Deinococcus Radiodurans ◽

Single Photon ◽

Photon Emission ◽

Emission Computed Tomography ◽

Cellulose Acetate Membrane ◽

High Concentration ◽

Iodine Species ◽

Radiation Resistant

Increasing concerns regarding the adverse effects of radioactive iodine waste have inspired the development of a highly efficient and sustainable desalination process for the treatment of radioactive iodine-contaminated water. Because of the high affinity of silver towards iodine species, silver nanoparticles immobilized on a cellulose acetate membrane (Ag-CAM) and biogenic silver nanoparticles containing the radiation-resistant bacterium Deinococcus radiodurans (Ag-DR) were developed and investigated for desalination performance in removing radioactive iodines from water. A simple filtration of radioactive iodine using Ag-CAM under continuous in-flow conditions (approximately 1.5 mL/s) provided an excellent removal efficiency (>99%) as well as iodide anion-selectivity. In the bioremediation study, the radioactive iodine was rapidly captured by Ag-DR in the presence of high concentration of competing anions in a short time. The results from both procedures can be visualized by using single-photon emission computed tomography (SPECT) scanning. This work presents a promising desalination method for the removal of radioactive iodine and a practical application model for remediating radioelement-contaminated waters.

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