scholarly journals Combining ability and genetic divergence among tropical maize inbred lines using SSR markers

2021 ◽  
Vol 43 ◽  
pp. e53317
Author(s):  
Tauana Gibim Eisele ◽  
Dener Lazzari ◽  
Tereza Aparecida da Silva ◽  
Ronald José Barth Pinto ◽  
Robson Akira Matsuzaki ◽  
...  
Keyword(s):  
Grain Yield ◽  
Ssr Markers ◽  
Genetic Divergence ◽  
Combining Ability ◽  
Inbred Lines ◽  
Simultaneous Increase ◽  
Additive Effects ◽  
Tropical Maize ◽  
Maize Inbred Lines ◽  
Ear Height

Our objectives were to evaluate general and specific combining ability (SCA) and genetic divergence among tropical maize inbred lines using single sequence repeat (SSR) markers. Thirteen inbred lines were crossed based on a complete diallel scheme. Hybrids and three checks were evaluated in a lattice experimental design. Silk and anthesis flowering, average plant height, average ear height, white spot (Pantoea ananatis) and gray leaf spot (Cercospora zeae-maydis) severity, and grain yield were evaluated. Significant differences (p < 0.05) for general and specific combining abilities were observed for all traits. Based on additive effects, inbred lines 1 (Flash) and 12 (SG 6015) were selected to reduce the flowering period and plant and ear height. Inbred lines 2 (CD 303) and 3 (AG 8080) were selected to reduce disease severity. For the simultaneous increase in grain yield and reduced severity of diseases, line 11 (AG 9090) as a parent or tester in topcross schemes is recommended. According to non-additive effects, crosses 2 (CD 303) × 13 (DKB 747) and 11 (AG 9090) × 12 (SG 6015) were selected for grain yield and future breeding programs. Six groups were identified using SSR markers; a major group contained six inbred lines. Because of the minor relationship between genetic divergence and SCA effects on grain yield limits, the use of the groups for future divergent crosses is recommended.

scholarly journals Partial diallel and genetic divergence analyses in maize inbred lines

2020 ◽  
Vol 43 ◽  
pp. e53540
Author(s):  
Maria Fernanda de Souza Dias Maioli ◽  
Ronald José Barth Pinto ◽  
Tereza Aparecida da Silva ◽  
Diego Ary Rizzardi ◽  
Robson Akira Matsuzaki ◽  
...  
Keyword(s):  
Grain Yield ◽  
Genetic Divergence ◽  
Block Design ◽  
Inbred Lines ◽  
Group Method ◽  
Arithmetic Average ◽  
Pair Group ◽  
Maize Inbred Lines ◽  
Randomized Block Design ◽  
Partial Diallel

In this study, we aimed to estimate general and specific combining abilities (GCA and SCA, respectively) and to verify genetic divergence (Rogers distance, Unweighted Pair-Group Method Using Arithmetic Average - UPGMA) using microsatellite markers in maize inbred lines. Using a partial diallel scheme, a total of 19 inbred lines were crossed as (9 x 10), which were derived from the single hybrids SG6015 and P30F53, respectively. The 90 hybrids were evaluated in an incomplete randomized block design with common checks and three replications during the 2017-2018 growing season. Flowering time, average plant height, ear height insertion, average ear diameter, ear length, number of lodged and broken plants, mass of 100 grains and grain yield were measured. According to the analysis of variance, GCA, and SCA were significant (p < 0.05) in all the measured traits; inbred line B as well as 1 and 8, derived from the single hybrids SG6015 and P30F53, respectively, were selected due to their higher GCA values in grain yield to be used in crosses as testers, while the single cross hybrid (B x 1) was selected due to their higher SCA value in grain yield to be used in future breeding programs. The molecular marker analysis divided the inbred lines into two groups, where the highest dissimilarity (0.74) was observed between lines A and 9; however, these did not result in a high SCA value, therefore the hybrids obtained by such crossings were not selected for grain yield.

Combining Ability, Genetic Distances, and Heterosis among Elite CIMMYT and IITA Tropical Maize Inbred Lines

Crop Science ◽  
2009 ◽  
Vol 49 (4) ◽  
pp. 1201-1210 ◽  
Author(s):  
Thanda Dhliwayo ◽  
Kevin Pixley ◽  
Abebe Menkir ◽  
Marilyn Warburton
Keyword(s):  
Combining Ability ◽  
Inbred Lines ◽  
Genetic Distances ◽  
Tropical Maize ◽  
Maize Inbred Lines

Heterosis and combining ability for grain yield and its components in selected maize inbred lines

2007 ◽  
Vol 24 (3) ◽  
pp. 133-137 ◽  
Author(s):  
D. W. Gissa ◽  
H. Zelleke ◽  
M. T. Labuschagne ◽  
T. Hussien ◽  
H. Singh
Keyword(s):  
Grain Yield ◽  
Combining Ability ◽  
Inbred Lines ◽  
Maize Inbred Lines

scholarly journals Combining Ability Study for Grain Yield and Agronomic Traits of Quality Protein Maize (Zea mays L.) Inbred Lines Adapted to Mid-Altitude Agroecology of Ethiopia

2021 ◽  
Vol 4 (3) ◽  
pp. 286-304
Author(s):  
Lemi Mideksa Yadesa ◽  
Sentayehu Alamerew ◽  
Berhanu Tadesse
Keyword(s):  
Grain Yield ◽  
Combining Ability ◽  
Agronomic Traits ◽  
Inbred Lines ◽  
Quality Protein Maize ◽  
Lattice Design ◽  
Maize Cultivars ◽  
Maize Inbred Lines ◽  
Maize Varieties ◽  
Almost All

In spite of the importance of quality protein maize to alleviate protein deficiency, almost all maize varieties cultivated in Ethiopia are normal maize varieties, which are devoid of lysine and tryptophan. Perusing the combining ability of QPM inbred for grain yield and its components is vital to design appropriate breeding strategies for the development of nutritionally enhanced maize cultivars. A line x tester analysis involving 36 crosses generated by crossing 9  elite maize inbred lines with 4 testers were evaluated for different desirable agronomic traits during the 2019 main season at BNMRC and JARC. The experiment was conducted using alpha lattice design with 3 replications. The objectives were to determine the combining ability of quality protein maize inbred lines, adapted to mid altitude agroecology of Ethiopia for agronomic traits. The crosses were evaluated in alpha lattice design replicated 3 times. Analyses of variances showed significant mean squares due to crosses for almost all the traits studied. GCA mean squares due to lines and testers were significant (P<0.05 or P<0.01) for most studied traits. SCA mean squares were also significant for most attributes across locations. The comparative importance of GCA and SCA variances observed in the current study for most studied traits indicated the preponderance of additive genetic variance in governing these attributes. Only L3 was the best general combiner for grain yield. Inbred line L3, for days to anthesis and L5 for days to silking had negative and significant GCA effects. L5 and L6 displayed negative and significant GCA effects for plant and ear height. Crosses, L2xT4, L3xT4, L4xT4, L5xT2, L6xT3, L7xT2, L9xT1 and L9xT4 were good specific combiners for grain yield. In general, these genotypes help as a source of promising alleles that could be used for forthcoming breeding work in the development of quality protein maize cultivars with desirable traits.

scholarly journals Genetic Diversity and Heterotic Orientation of South Africa Maize Inbred Lines Towards Tropical and Temperate Testers

2021 ◽  
Author(s):  
Siphiwokuhle Funani Shandu ◽  
John Derera ◽  
Kingston Mashingaidze ◽  
Edmore Gasura
Keyword(s):  
South Africa ◽  
Genetic Diversity ◽  
Grain Yield ◽  
Combining Ability ◽  
Molecular Genetic ◽  
Inbred Lines ◽  
Genetic Distances ◽  
Specific Combining Ability ◽  
Heterotic Groups ◽  
Maize Inbred Lines

Abstract An efficient hybrid breeding program defines and utilizes few heterotic groups. The objectives of this study were to determine genetic diversity and alignment of South Africa maize inbred lines collection towards tropical and temperate testers. Forty-two maize inbred lines were genotyped with 56110 single nucleotide polymorphism (SNP) DNA markers, using the Illumina MaizeSNP50 Bead chip. The 42 lines were crossed to two tropical and two temperate inbred line testers. The testcrosses were evaluated across seven environments, in South Africa, during 2014-2016. Genotypes and specific combining ability (SCA) effects of hybrids were significantly different (P<0.05) for grain yield. There was a weak correlation between molecular genetic distances and both grain yield mean and specific combining ability effects of hybrids, indicating that productivity of maize inbred lines could not be reliably determined based on molecular genetic distances. The SCA data was capable of classifying these maize inbred lines into three heterotic groups with respect to both tropical and temperate testers. Only a few lines could not be grouped on the basis of SCA data. The study also indicated high level of diversity among the maize inbred lines, which was shown by both the dendogram and molecular genetic distances. The SNP marker data classified the inbred lines into 11 clusters that could be simplified into three major groups of normal maize endosperm and two groups of quality protein maize (QPM) endosperm types. However, the SNP data indicated that maize lines were more aligned towards tropical than temperate inbred testers. This information would be useful for simplifying heterotic classification of the lines with profound implications for breeding progress.

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