scholarly journals Effects of glutathione supplementation and carbon source during somatic embryogenesis of Acca sellowiana (O.Berg) Burret (Myrtaceae)

2018 ◽  
Vol 40 (1) ◽  
pp. 40257
Author(s):  
Ana Flávia Pavei ◽  
Hugo Pacheco de Freitas Fraga ◽  
Leila Do Nascimento Vieira ◽  
Miguel Pedro Guerra
Keyword(s):  
Somatic Embryogenesis ◽  
Carbon Source ◽  
Acca Sellowiana

5-Azacytidine combined with 2,4-D improves somatic embryogenesis of Acca sellowiana (O. Berg) Burret by means of changes in global DNA methylation levels

2012 ◽  
Vol 31 (12) ◽  
pp. 2165-2176 ◽  
Author(s):  
Hugo P. F. Fraga ◽  
Leila N. Vieira ◽  
Clarissa A. Caprestano ◽  
Douglas A. Steinmacher ◽  
Gustavo A. Micke ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Somatic Embryogenesis ◽  
Global Dna Methylation ◽  
Acca Sellowiana

scholarly journals Effects of Carbon Source and Explant Type on Somatic Embryogenesis of Four Cacao Genotypes

HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 753-758 ◽  
Author(s):  
Abdoulaye Traore ◽  
Mark J. Guiltinan
Keyword(s):  
Somatic Embryogenesis ◽  
Carbon Source ◽  
Somatic Embryos ◽  
Slow Growth ◽  
Carbon Sources ◽  
The Other ◽  
Root Production ◽  
Explant Type ◽  
Embryo Maturation ◽  
Genotypic Effect

The effects of five carbon sources (glucose, fructose, maltose, sorbitol, and sucrose) and two explant types (petals and staminodes) on cacao somatic embryogenesis was studied. No growth was observed on both types of explants cultured on sorbitol containing media and slow growth was obtained on media supplemented with maltose. Depending on the genotype, the percentage of explants producing one or more embryos ranged from 6% to 99%, 18% to 98%, and 3% to 82% on media containing glucose, fructose and sucrose respectively. Explants cultured continuously on maltose or sorbitol-containing media failed to produce embryos. Staminode explants produced 3 to 10 times more somatic embryos than petals. A strong genotypic effect on somatic embryogenesis was observed. Staminode explants of the Forastero clones Laranja and PSUSca 6 produced 2 to 30 times more somatic embryos than the Trinitarios UF 613 and ICS 16. During embryo maturation and conversion, no significant differences were observed among glucose, fructose, maltose, or sucrose for embryo weight, total shoot and root production. However, we found that all plantlets produced on glucose had shoots with normal cacao leaves while the other carbon sources sometimes produced plantlets with cotyledon-like leaves.

scholarly journals The role of γ -aminobutyric acid (Gaba) in somatic embryogenesis of Acca sellowiana Berg. (Myrtaceae)

2009 ◽  
Vol 21 (4) ◽  
pp. 271-280 ◽  
Author(s):  
Maristela Raitz Booz ◽  
Gilberto B. Kerbauy ◽  
Miguel Pedro Guerra ◽  
Rosete Pescador
Keyword(s):  
Somatic Embryogenesis ◽  
High Frequency ◽  
Somatic Embryos ◽  
Culture Medium ◽  
Aminobutyric Acid ◽  
Zygotic Embryos ◽  
Protein Amino Acid ◽  
Endogenous Gaba ◽  
Acca Sellowiana

The γ-aminobutyric acid (Gaba) is a non-protein amino acid found in prokaryotes and eukaryotes. Its role in plant development has not been fully established. This study reports a quantification of the levels of endogenous Gaba, as well as investigation of its role in different stages of somatic embryogenesis in Acca sellowiana Berg. (Myrtaceae). Zygotic embryos were used as explants and they were inoculated into the culture medium contained different concentrations of Gaba (0,2, 4, 6, 8 and 10 µM). The highest concentrations of endogenous Gaba were detected between the third and nine days after inoculation, reaching the value of 12.77 µmol.g-1FW. High frequency of somatic embryogenesis was observed in response to 10 µM Gaba. This treatment also resulted in a large number of normal embryos, and the lowest percentage of formation of fused somatic embryos, phenotypic characteristic of most deformed embryos in all treatments. Also, all treatments promoted the formation of the somatic embryos with positive characteristics of development resumption, which however did not originate the seedlings.

Dynamics of free and3H-labelled glutamine concentrations during zygotic and somatic embryogenesis of Feijoa [Acca sellowiana(O. Berg.) Burret]

2012 ◽  
Vol 87 (6) ◽  
pp. 583-587 ◽  
Author(s):  
R. Pescador ◽  
G. B. Kerbauy ◽  
H.P.F. Fraga ◽  
R. M. Hamasaki ◽  
L.B.B. Tavares ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Acca Sellowiana

scholarly journals Somatic embryogenesis and plant regeneration in elite clones of Theobroma cacao

2008 ◽  
Vol 43 (10) ◽  
pp. 1433-1436 ◽  
Author(s):  
Thiago Édson Ribeiro da Silva ◽  
Luciana Cardoso Cidade ◽  
Fátima Cerqueira Alvim ◽  
Júlio Cézar de Mattos Cascardo ◽  
Marcio Gilberto Cardoso Costa
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Carbon Source ◽  
Embryogenic Callus ◽  
Theobroma Cacao ◽  
Somatic Embryos ◽  
Embryogenic Calli ◽  
Embryogenic Potential ◽  
Petri Dishes ◽  
Theobroma Cacao L

The objective of this work was to evaluated a procedure for somatic embryogenesis and regeneration of cacao (Theobroma cacao L.) elite clones. Petal explants from cacao clones TSH 565 and TSH 1188 were cultured on PCG and SCG-2 media, for calli growth. Somatic embryos were formed on the surface of embryogenic calli after transfer to embryo development (ED) medium. Clone TSH 565 showed a higher embryogenic potential than TSH 1188. The best combination of carbon source for embryo induction in ED medium was genotype-specific. Embryogenic callus formations increased in micropore tape-sealed Petri dishes, irrespective of cacao genotype. Mature somatic embryos were successfully converted into plantlets.

Improvements in somatic embryogenesis protocol in Feijoa (Acca sellowiana (Berg) Burret): Induction, conversion and synthetic seeds

2007 ◽  
Vol 111 (3) ◽  
pp. 228-234 ◽  
Author(s):  
Gabriela Claudia Cangahuala-Inocente ◽  
Lírio Luiz Dal Vesco ◽  
Douglas Steinmacher ◽  
Antonio Carlos Torres ◽  
Miguel Pedro Guerra
Keyword(s):  
Somatic Embryogenesis ◽  
Synthetic Seeds ◽  
Acca Sellowiana

Advances and constraints in somatic embryogenesis of Araucaria angustifolia, Acca sellowiana, and Bactris gasipaes

2020 ◽  
Vol 143 (2) ◽  
pp. 241-263
Author(s):  
Valdir Marcos Stefenon ◽  
Joseph Francis Ree ◽  
Marcos Vinicius Marques Pinheiro ◽  
Daniela Goeten ◽  
Neusa Steiner ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Bactris Gasipaes ◽  
Araucaria Angustifolia ◽  
Acca Sellowiana

scholarly journals Patterns of protein and carbohydrate accumulation during somatic embryogenesis of Acca sellowiana

2009 ◽  
Vol 44 (3) ◽  
pp. 217-224 ◽  
Author(s):  
Gabriela Claudia Cangahuala-Inocente ◽  
Neusa Steiner ◽  
Sara Beatriz Maldonado ◽  
Miguel Pedro Guerra
Keyword(s):  
Somatic Embryogenesis ◽  
Total Protein ◽  
Somatic Embryos ◽  
Periodic Acid ◽  
Soluble Sugars ◽  
Buffer Solution ◽  
Periodic Acid Schiff ◽  
Total Sugars ◽  
Protein Levels ◽  
Acca Sellowiana

The aim of this work was to quantify the protein, starch and total sugars levels during histodifferentiation and development of somatic embryos of Acca sellowiana Berg. For histological observations, the samples were dehydrated in a battery of ethanol, embedded in historesin and stained with toluidine blue (morphology), coomassie blue (protein bodies) and periodic acid-Schiff (starch). Proteins were extracted using a buffer solution, precipitated using ethanol and quantified using the Bradford reagent. Total sugars were extracted using a methanol-chloroform-water (12:5:3) solution and quantified by a reaction with anthrone at 0.2%. Starch was extracted using a 30% perchloric acid solution and quantified by a reaction with anthrone at 0.2%. During the somatic embryogenesis' in vitro morphogenesis and differentiation processes, the total protein levels decreased and the soluble sugars levels increased during the first 30 days in culture and remained stable until the 120th day. On the other hand, total protein levels increased according to the progression in the developmental stages of the somatic embryos. The levels of total sugars and starch increased in the heart and cotyledonary stages, and decreased in the torpedo and pre-cotyledonary stages. These compounds play a central role in the development of somatic embryos of Acca sellowiana.

scholarly journals Efeito da fonte de carbono na embriogênese somática em Bactris gasipaes

2016 ◽  
Vol 36 (86) ◽  
pp. 179
Author(s):  
Gisela Manuela de França Bettencourt ◽  
Laudiane Bruna Zanella ◽  
Marguerite Germaine Ghislaine Quoirin ◽  
Juliana Degenhardt-Goldbach
Keyword(s):  
Somatic Embryogenesis ◽  
Carbon Source ◽  
Somatic Embryos ◽  
Cell Layer ◽  
Bactris Gasipaes ◽  
Thin Cell Layer ◽  
Embryogenic Calli ◽  
Meristematic Region ◽  
Somatic Embryogenic

A metodologia de camada fina de células foi utilizada com sucesso para indução de embriogênese somática em região do meristema apical de plantas de pupunha mantidas em casa-de-vegetação. O efeito de três fontes de carbono: sacarose, glicose e manitol na indução de embriogênese somática foi avaliado. Embriões somáticos foram observados após a indução apenas nos meios contendo sacarose ou glicose em igual proporção.Effect of carbon source on somatic embryogenesis of Bactris gasipaesThin cell layer of meristematic region was successfully used to induce somatic embryogenesis in greenhouse-grown plants of pejibaye. The effects of three sources of carbohydrates, sucrose, glucose and mannitol on induction of somatic embryogenic calli were evaluated. Somatic embryos were observed on media containing either sucrose or glucose.Index terms: Thin cell layer, Glucose, Sucrose

A hormonal misunderstanding in Acca sellowiana embryogenesis: levels of zygotic embryogenesis do not match those of somatic embryogenesis

2012 ◽  
Vol 68 (1) ◽  
pp. 67-76 ◽  
Author(s):  
Rosete Pescador ◽  
Gilberto Barbante Kerbauy ◽  
Wagner de Melo Ferreira ◽  
Eduardo Purgatto ◽  
Rogério Mamoru Suzuki ◽  
...  
Keyword(s):  
Somatic Embryogenesis ◽  
Zygotic Embryogenesis ◽  
Acca Sellowiana
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