Protein Adsorption Property of a Biomimetic Apatite Coating

2005 ◽  
pp. 403-406 ◽  
Author(s):  
Hai Bo Wen ◽  
Elizabeth A. Hippensteel ◽  
Pan Jian Li
Keyword(s):  
Protein Adsorption ◽  
Adsorption Property ◽  
Biomimetic Apatite ◽  
Apatite Coating

Protein Adsorption Property of a Biomimetic Apatite Coating

2005 ◽  
Vol 284-286 ◽  
pp. 403-406 ◽  
Author(s):  
Hai Bo Wen ◽  
Elizabeth A. Hippensteel ◽  
Pan Jian Li
Keyword(s):  
Growth Factor ◽  
Protein Adsorption ◽  
Soft Tissues ◽  
Adsorption Property ◽  
Calf Serum ◽  
Therapeutic Agents ◽  
Nanoporous Structure ◽  
Ha Coating ◽  
Biomimetic Apatite ◽  
Tgf Β1

Hydroxyapatite (HA) coating applied on metallic orthopedic joint implants can improve bone apposition, presumably through selective protein adsorption from blood plasma. However, the detailed interaction mechanism of HA coating with serum proteins remains to be largely elucidated. Protein adsorption behavior of a biomimetic apatite (BAp) coating in bovine calf serum and alpha calf fraction was investigated in this study. Plasma sprayed HA (PSHA) coating was tested in alpha calf fraction. The microstructure and composition of the coatings before and after serum incubation were characterized and the proteins adsorbed during the incubation were extracted from the coatings and analyzed. Microstructural transformation of the BAp coating accompanied by selective serum protein adsorption was observed after incubation in both media. The total protein amount adsorbed by the BAp coating in alpha calf faction was about three times that of the PSHA coating. To test the potential use of BAp coating as a carrier of therapeutic agents, interaction between the BAp coating and transforming growth factor (TGF)-β1 was studied. The growth factor was successfully loaded onto the coating in a sodium acetate buffer. Because of its high affinity to the coating, TGF-β1 could not be easily eluted in a bovine serum albumin containing solution but could be recovered after coating dissolution in acid. The strong protein adsorption property of the BAp coating was found to be due mainly to its unique nanoporous structure. The BAp coating can serve as an ideal carrier of therapeutic agents for aiding in the healing of bone and soft tissues.

Biomimetic apatite coating on Mg-PSZ/Al2O3 composites. Effect of the immersion method

2005 ◽  
Vol 59 (11) ◽  
pp. 1352-1355 ◽  
Author(s):  
D.A. Cortes ◽  
A.A. Nogiwa ◽  
J.M. Almanza ◽  
S. Ortega
Keyword(s):  
Immersion Method ◽  
Biomimetic Apatite ◽  
Apatite Coating

Conformational Change of Protein due to Contact with Bioceramic Materials

2005 ◽  
Vol 284-286 ◽  
pp. 517-520 ◽  
Author(s):  
Satoshi Nagai ◽  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Akiyoshi Osaka
Keyword(s):  
Structural Change ◽  
Secondary Structure ◽  
Bovine Serum Albumin ◽  
Protein Adsorption ◽  
Tricalcium Phosphate ◽  
Bovine Serum ◽  
Adsorption Property ◽  
Cd Spectroscopy ◽  
Uv Spectra ◽  
Interaction Forces

The structural change of bovine serum albumin (BSA) was analyzed by means of ultraviolet (UV) and circular dichroism (CD) spectroscopy due to in contact with ceramics powders such as Al2O3, SiO2, TiO2, ZrO2, hydroxyapatite and b-tricalcium phosphate. The absorbance of the UV spectra increased for the BSA solution in contact with TiO2, ZrO2 and Al2O3, that is, the BSA solution clouded. This indicated the aggregation of BSA after contact with those ceramics powders. The CD spectroscopy showed that the ordered secondary structure of BSA was diminished on contact with TiO2 and ZrO2. We discussed the protein adsorption property of ceramics on the basis of the magnitude of the interaction forces between proteins and the bioceramics.

Selective Protein Adsorption Property and Structure of Nano-Crystalline Hydroxy-Carbonate Apatite

2006 ◽  
Vol 309-311 ◽  
pp. 503-506 ◽  
Author(s):  
Satoshi Hayakawa ◽  
K. Ohnishi ◽  
Kanji Tsuru ◽  
Akiyoshi Osaka ◽  
Eiji Fujii ◽  
...  
Keyword(s):  
Surface Charge ◽  
Protein Adsorption ◽  
Adsorption Property ◽  
Considerable Change ◽  
Mas Nmr ◽  
Carbonate Apatite ◽  
Oh Groups ◽  
Carbonate Ions ◽  
Cp Mas Nmr ◽  
Hydroxy Carbonate

The selective protein adsorption property and the local structure around carbonate ions of nanocrystalline hydroxy-carbonate apatite were examined in this study. Considerable change in the selectivity in the adsorption of BSA and β2-MG was observed due to the incorporation of thecarbonate ions in hydroxyapatite lattice. Since the protein adsorption property seems to be related to the surface charge density of hydroxyapatite due to the carbonation, the chemical states of the incorporated carbonate ions were examined by the 31C CP-MAS NMR spectroscopy. At least four peaks assignable to carbonate ions in A-site(OH-) and B-site(PO4 3-) were observed in 13C CP-MAS NMR spectrum. Thus, we must take into consideration that the surface charge distribution and the decrement of polar groups such as OH- groups due to the distribution of carbonate ions in both Aand B-sites of the hydroxyapatite lattice are particularly favorable for β2-MG adsorption rather than for BSA adsorption.

Fabrication of biomimetic apatite coating on porous titanium and their osteointegration in femurs of dogs

2010 ◽  
Vol 30 (1) ◽  
pp. 98-104 ◽  
Author(s):  
C.Y. Zhao ◽  
X.D. Zhu ◽  
T. Yuan ◽  
H.S. Fan ◽  
X.D. Zhang
Keyword(s):  
Porous Titanium ◽  
Biomimetic Apatite ◽  
Apatite Coating

Selective protein adsorption property and characterization of nano-crystalline zinc-containing hydroxyapatite

2006 ◽  
Vol 2 (1) ◽  
pp. 69-74 ◽  
Author(s):  
Eiji Fujii ◽  
Mizuki Ohkubo ◽  
Kanji Tsuru ◽  
Satoshi Hayakawa ◽  
Akiyoshi Osaka ◽  
...  
Keyword(s):  
Protein Adsorption ◽  
Adsorption Property ◽  
Nano Crystalline

Controlling the Biodegradation Rate of AZ31 with Biomimetic Apatite Coating

2013 ◽  
Vol 821-822 ◽  
pp. 1047-1050 ◽  
Author(s):  
Ya Jing Zhang ◽  
Xiao Hui Xi ◽  
Hai Long Jia ◽  
Dan Zhang
Keyword(s):  
Body Fluid ◽  
Degradation Rate ◽  
Biodegradable Material ◽  
Biodegradation Rate ◽  
Natural Bone ◽  
Ha Coating ◽  
Biomimetic Apatite ◽  
Apatite Coating ◽  
Degradation Test ◽  
Orthopedic Applications

Magnesium alloys have the potential as a biodegradable material for orthopedic applications. However, severely corrosion in a physiological environment limits the clinical application of Mg alloys. Hydroxyapatite is the main composition of natural bone, it has excellent bioactivity and osteoconductivity. In this paper, the samples of AZ31 were ultrasonically cleaned using distilled water, and then the coating was formed on the surfaces of the samples by biomimetic process. The samples were subsequently immersed in the simulated body fluid (SBF) for the degradation test. The coatings were characterized by XRD, EDS and SEM. The results showed that hydroxyapatite (HA) coating was formed on the surface of AZ31 after 24h by biomimetic process; the HA coating greatly decreased the corrosion rate of AZ31 substrate; the degradation rate of samples can be controlled by closely adjusting the hydroxyapatite coating.

Effect of Biomimetic Nanocrystalline Apatite Surface on Bone Ingrowth

2012 ◽  
Vol 12 ◽  
pp. 41-50
Author(s):  
Jonathan Gelber ◽  
Juan C. Hermida ◽  
Shantanu Patil ◽  
Clifford W. Colwell ◽  
Darryl D. D'Lima
Keyword(s):  
Bone Ingrowth ◽  
Solution Deposition ◽  
Particle Generation ◽  
Biomimetic Apatite ◽  
Hydroxyapatite Coatings ◽  
Bone Response ◽  
Apatite Coating ◽  
Coating Delamination ◽  
Group A ◽  
Plasma Sprayed

The Success of Hydroxyapatite-Coated Acetabular Components Has Not Been Consistent. Plasma-Sprayed Hydroxyapatite Coatings Work Well on Nonporous Substrates but Do Not Coat the Inner Surfaces of Open-Porous Substrates. Solution Deposition Can Generate Consistent Bioceramic Coats on Porous Surfaces that More Closely Mimic the Trabecular Pattern and Biochemistry at the Bone Interface. we Compared Bone Response to the Following Implants: Porous-Coated Ti6al4v Cylinders with 1 of 3 Treatments: Plasma Sprayed with Hydroxyapatite (HA), Coated with a Solution-Deposited Biomimetic Apatite Coating (BA), and Untreated (Control). Bilateral Femurs in 36 Rabbits Were Implanted with One of the above Implants. Bone Ingrowth for HA and BA Surfaces Was Significantly Higher than that for Control Surfaces. No Fragmentation or Debris Production Was Evident in the Apatite Coat of the BA Group. A Biomimetic Coat of Solution-Deposited Apatite May Be Resistant to Coating Delamination and Particle Generation.

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