The Effect of Thermal Contact Resistance on Heat Management in a Shuttling PCR System

2013 ◽  
Vol 284-287 ◽  
pp. 1941-1945
Author(s):  
Jyh Jian Chen ◽  
Wei Hua Chen ◽  
Yi Shiang Shie

A novel shuttling polymerase chain reaction (PCR) system is assembled to make temperature uniform in the reaction chamber. The chamber is oscillated by a servo motor and contacted with three different isothermal zones to complete several thermal cycles. The home-made computer code is utilized to investigate the influences of operational parameters on the temperature inside the chamber. Numerical results show that the contact resistances between the heating blocks and the reaction chamber dominate the temperatures inside the PCR chamber. In this work a PCR system that is composed of the PID controller, the moving stage, three aluminum blocks for three different isothermal zones and a reaction chamber is also developed. Experimental results demonstrated that the stability of this shuttling PCR system is confirmed. And results show that DNA templates provided with the yT&A® cloning vector are amplified successfully in this PCR system.

1981 ◽  
Vol 48 (3) ◽  
pp. 555-558 ◽  
Author(s):  
J. R. Barber

A perturbation method is used to investigate the stability of a simple one-dimensional rod model of thermoelastic contact which exhibits multiple steady-state solutions. A thermal contact resistance is postulated which is a continuous function of the contact pressure or separation. It is found that solutions involving substantial separation and/or contact pressures are always stable, but these are separated by unstable “imperfect contact” solutions in which one of the rods is very lightly loaded or has a very small separation. The results can be expressed in terms of the minimization of a certain energy function.


1990 ◽  
Vol 57 (2) ◽  
pp. 365-369 ◽  
Author(s):  
Ronggang Zhang ◽  
J. R. Barber

When heat is conducted across an interface between two different materials, the interaction between thermoelastic distortion and thermal contact resistance can cause the system to be unstable. This paper investigates the influence of material properties on the stability criterion for an interface between two half planes. It is found that most material combinations exhibit one or other of two kinds of stability behavior. In one of these, the stability criterion is closely related to that for uniqueness of the steady-state solution and instability is only possible for one direction of heat flow. In the other, instability can occur for either direction of heat flow and in one case is characterized by the oscillatory growth of a pressure perturbation.


2015 ◽  
Vol 83 (2) ◽  
Author(s):  
Jia-Jia Mao ◽  
Liao-Liang Ke ◽  
Yue-Sheng Wang ◽  
Jing Liu

This paper investigates the frictionally excited thermoelastic instability (TEI) of a functionally graded material (FGM) half-plane sliding against a homogeneous half-plane at the out-of-plane direction with the thermal contact resistance. A uniform pressure presses these two half-planes together. The material properties of FGMs are assumed to be varied as an exponential form. Using the perturbation method, we derive the characteristic equation for the TEI problem to solve the unknown critical heat flux and critical sliding speed. The effects of the thermal contact resistance, gradient index, friction coefficient, and heat generation factor on the stability boundaries are discussed for four different material combinations. The results may provide a possible method to improve the contact stability in the sliding system by using FGMs.


2020 ◽  
Vol 27 (7) ◽  
pp. 617-627
Author(s):  
Yuanyuan Tian ◽  
Mengjun Zhang ◽  
Junli Wang ◽  
Anbang Liu ◽  
Huaqing Xie ◽  
...  

Small ◽  
2021 ◽  
pp. 2102128
Author(s):  
Taehun Kim ◽  
Seongkyun Kim ◽  
Eungchul Kim ◽  
Taesung Kim ◽  
Jungwan Cho ◽  
...  

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