Development of the Reliable Fluorometric Assay for the Sensitive Determination of Nitric Oxide Metabolites in Biological Fluids

2013 ◽  
Vol 477-478 ◽  
pp. 1359-1362
Author(s):  
Qiao Yang ◽  
Xiao Ling Zhang ◽  
Hong Liang Huang
Keyword(s):  
Nitric Oxide ◽  
Biological Fluids ◽  
Rapid Screening ◽  
Detection Methods ◽  
Clinical Samples ◽  
No Production ◽  
Fluorescence Measurements ◽  
Large Numbers ◽  
Nitric Oxide Metabolites

In this study, we developed a fluorescence measurements in microplates for high-throughput determination of nitric oxide (NO) metabolites, which is used as a reliable and sensitive marker of NO production, in biological fluids using a pH-independent fluorescence increasing probe, DAMBO-PH. The sensitivity of this method is lower than that of the other detection methods reported in the literature. This method was demonstrated to be useful for the rapid screening of large numbers of biological and clinical samples, and should be an important tool for further clarifying the physiology, pathology and pharmacology roles of NO.

Sample stacking – Capillary electrophoretic determination of nitrate and nitrite contents as nitric oxide metabolites in honey varieties originated from Anatolia

2021 ◽  
Author(s):  
J. Aftab ◽  
Z. Kalaycıoğlu ◽  
S. Kolaylı ◽  
F.B. Erim
Keyword(s):  
Nitric Oxide ◽  
No Production ◽  
Nitrate Anion ◽  
Sample Stacking ◽  
Beneficial Effects ◽  
Capillary Electrophoretic ◽  
Nitric Oxide Metabolites ◽  
Nitrate And Nitrite ◽  
Electrophoresis Technique

Abstract Nitrate and nitrite ions taken from food are the sources of bioavailable nitric oxide (NO) in the nitrogen cycle. Some beneficial effects of honey on health are attributed to the ability of honey to increase NO production. The variation of nitrate and nitrite levels of honey samples collected from different Anatolia regions were clarified using capillary electrophoresis technique. The sensitivities of both anions were improved with the application of the sample stacking method. Separation buffer consisted of 30 mmol L−1 formic acid and 30 mmol L−1 sodium sulfate at a pH of 4.0. The CE technique revealed that 18 honey samples contained nitrate anion ranged between 2.53 and 31.8 mg kg−1. Nitrite amounts were found in lower amounts in the honey samples as between non-detected and 0.533 mg kg−1. The observed differences in nitrate levels between honey varieties may be a way to determine honey's origin.

scholarly journals In Vitro Study of Nitric Oxide Metabolites Effects on Human Hydatid ofEchinococcus granulosus

2009 ◽  
Vol 2009 ◽  
pp. 1-7 ◽  
Author(s):  
Razika Zeghir-Bouteldja ◽  
Manel Amri ◽  
Saliha Aitaissa ◽  
Samia Bouaziz ◽  
Dalila Mezioug ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Hydatid Cyst ◽  
Echinococcus Granulosus ◽  
In Vitro Study ◽  
No Production ◽  
In Vitro Effects ◽  
Nitric Oxide Metabolites

Hydatidosis is characterized by the long-term coexistence of larvaEchinococcus granulosusand its host without effective rejection. Previous studies demonstrated nitric oxide (NO) production (in vivo and in vitro) during hydatidosis. In this study, we investigated the direct in vitro effects of NO species: nitrite (NO2−), nitrate (NO3−) and peroxynitrite (ONOO−) on protoscolices (PSCs) viability and hydatid cyst layers integrity for 24 hours and 48 hours. Our results showed protoscolicidal activity ofNO2−andONOO−24 hours and 3 hours after treatment with 320 μM and 80 μM respectively. Degenerative effects were observed on germinal and laminated layers. The comparison of the in vitro effects of NO species on the PSCs viability indicated thatONOO−is more cytotoxic thanNO2−. In contrast,NO3−has no effect. These results suggest possible involvement ofNO2−andONOO−in antihydatic action and point the efficacy of these metabolites as scolicidal agents.

Rapid Screening Procedure for the Determination of Preservatives in Ground Beef: Sulfites, Benzoates, Sorbates, and Ascorbates

1976 ◽  
Vol 59 (4) ◽  
pp. 766-769
Author(s):  
Albert B Karasz ◽  
John J Maxstadt ◽  
Juergen Reher ◽  
Frank Decocco
Keyword(s):  
Ascorbic Acid ◽  
Quantitative Determination ◽  
Sodium Sulfite ◽  
Limit Of Detection ◽  
Ground Beef ◽  
Rapid Screening ◽  
Sodium Ascorbate ◽  
Potassium Sorbate ◽  
Large Numbers

Abstract A procedure is described for the examination of large numbers of ground beef samples for the 4 most commonly used preservatives. A single sample solution is prepared and aliquots are taken for the various tests. Sulfite is determined colorimetrically with acid-bleached p-rosaniline. Ascorbic acid is titrated with 2,6-dichloroindophenol. Benzoic and sorbic acids are measured by ultraviolet absorption. Negative samples are rapidly sorted out in this procedure and positive samples are completed for quantitative determination of the preservative. Recoveries are 95% for sodium sulfite, 103% for sodium benzoate, 90% for potassium sorbate, and 81 % expected recovery for sodium ascorbate. The limit of detection is 0.005% for potassium sorbate, and 0.01% for the 3 other preservatives.

scholarly journals Determination of dissolved nitric oxide in coastal waters of the Yellow Sea off Qingdao

2017 ◽  
Author(s):  
Chun-Ying Liu ◽  
Wei-Hua Feng ◽  
Ye Tian ◽  
Gui-Peng Yang ◽  
Pei-Feng Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
Coastal Waters ◽  
Yellow Sea ◽  
The Yellow Sea ◽  
No Production ◽  
Spatial Distributions ◽  
Production Rates ◽  
Temporal And Spatial

Abstract. We developed a new method for the determination of dissolved nitric oxide (NO) in discrete seawater samples based on a combination of a purge-and-trap set-up and fluorometric detection of NO. 2,3-diaminonaphthalene (DAN) reacts with NO in seawater to form the highly fluorescent 2,3-naphthotriazole (NAT). The fluorescence intensity was linear for NO concentrations in the range from 0.14 nmol L−1 to 19 nmol L−1. We determined a detection limit of 0.068 nmol L−1, an average recovery coefficient of 83.8 % (80.2–90.0 %), and a relative standard deviation of ±7.2 %. With our method we determined for the first time the temporal and spatial distributions of NO surface concentrations in coastal waters of the Yellow Sea off Qingdao and in Jiaozhou Bay during a cruise in November 2009. The concentrations of NO varied from below the detection limit to 0.50 nmol L−1 with an average of 0.26 ± 0.14 nmol L−1. NO surface concentrations were generally enhanced significantly during daytime implying that NO formation processes such as NO2− photolysis are much higher during daytime than chemical NO consumption which, in turn, lead to a significant decrease of NO concentrations during nighttime. In general, NO surface concentrations and measured NO production rates were higher compared to previously reported measurements. This might be caused by the high NO2− surface concentrations encountered during the cruise. Moreover, additional measurements of NO production rates implied that the occurrence of particles and a temperature increase can enhance NO production rates. With the method introduced here we have a reliable and comparably easy to use method at hand to measure oceanic NO surface concentrations which can be used to decipher both its temporal and spatial distributions as well as its biogeochemical pathways in the oceans.

Determination of NO production in melanoma cells using an amperometric nitric oxide sensor

1997 ◽  
Vol 43 (1) ◽  
pp. 19-25 ◽  
Author(s):  
O. Raveh ◽  
N. Peleg ◽  
A. Bettleheim ◽  
I. Silberman ◽  
J. Rishpon
Keyword(s):  
Nitric Oxide ◽  
Melanoma Cells ◽  
No Production ◽  
Nitric Oxide Sensor

A Rapid Quantitative lon-Exchange Extraction for 2,8-Dioxyadenine

1970 ◽  
Vol 16 (10) ◽  
pp. 861-863 ◽  
Author(s):  
Sandra H White ◽  
Michael R Loken ◽  
Charles E Shields
Keyword(s):  
Quantitative Method ◽  
Biological Fluids ◽  
Rapid Screening ◽  
Chromatographic Technique ◽  
Screening Assay ◽  
Standard Curve ◽  
Exchange Extraction

Abstract A rapid quantitative method for the determination of 2,8-dioxyadenine in biological fluids has been developed and evaluated in which a two-step HCI elution from Dowex-50W is used. The standard curve has a slope of 0.999 0.0647, and recovery is essentially 100%. In contrast to the two-day col-umn chromatographic technique previously avail-able, this procedure permits numerous determina-tions in less than an hour, which makes it appro-priate as a rapid screening assay for 2,8•dioxyade-nine whenever large doses of adenine are admin-istered. A variation of this procedure may be used as a semiquantitative test for adenine.

Electrochemical assay for the determination of nitric oxide metabolites using copper(II) chlorophyllin modified screen printed electrodes

2015 ◽  
Vol 478 ◽  
pp. 121-127 ◽  
Author(s):  
Murugesan Balamurugan ◽  
Thangamuthu Madasamy ◽  
Manickam Pandiaraj ◽  
Kalpana Bhargava ◽  
Niroj Kumar Sethy ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Electrochemical Assay ◽  
Screen Printed Electrodes ◽  
Nitric Oxide Metabolites ◽  
Screen Printed
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