scholarly journals Determination of dissolved nitric oxide in coastal waters of the Yellow Sea off Qingdao

2017 ◽  
Author(s):  
Chun-Ying Liu ◽  
Wei-Hua Feng ◽  
Ye Tian ◽  
Gui-Peng Yang ◽  
Pei-Feng Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
Coastal Waters ◽  
Yellow Sea ◽  
The Yellow Sea ◽  
No Production ◽  
Spatial Distributions ◽  
Production Rates ◽  
Temporal And Spatial

Abstract. We developed a new method for the determination of dissolved nitric oxide (NO) in discrete seawater samples based on a combination of a purge-and-trap set-up and fluorometric detection of NO. 2,3-diaminonaphthalene (DAN) reacts with NO in seawater to form the highly fluorescent 2,3-naphthotriazole (NAT). The fluorescence intensity was linear for NO concentrations in the range from 0.14 nmol L−1 to 19 nmol L−1. We determined a detection limit of 0.068 nmol L−1, an average recovery coefficient of 83.8 % (80.2–90.0 %), and a relative standard deviation of ±7.2 %. With our method we determined for the first time the temporal and spatial distributions of NO surface concentrations in coastal waters of the Yellow Sea off Qingdao and in Jiaozhou Bay during a cruise in November 2009. The concentrations of NO varied from below the detection limit to 0.50 nmol L−1 with an average of 0.26 ± 0.14 nmol L−1. NO surface concentrations were generally enhanced significantly during daytime implying that NO formation processes such as NO2− photolysis are much higher during daytime than chemical NO consumption which, in turn, lead to a significant decrease of NO concentrations during nighttime. In general, NO surface concentrations and measured NO production rates were higher compared to previously reported measurements. This might be caused by the high NO2− surface concentrations encountered during the cruise. Moreover, additional measurements of NO production rates implied that the occurrence of particles and a temperature increase can enhance NO production rates. With the method introduced here we have a reliable and comparably easy to use method at hand to measure oceanic NO surface concentrations which can be used to decipher both its temporal and spatial distributions as well as its biogeochemical pathways in the oceans.

scholarly journals Determination of dissolved nitric oxide in coastal waters of the Yellow Sea off Qingdao

Ocean Science ◽  
2017 ◽  
Vol 13 (4) ◽  
pp. 623-632 ◽  
Author(s):  
Chun-Ying Liu ◽  
Wei-Hua Feng ◽  
Ye Tian ◽  
Gui-Peng Yang ◽  
Pei-Feng Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
Coastal Waters ◽  
Yellow Sea ◽  
The Yellow Sea ◽  
No Production ◽  
Spatial Distributions ◽  
Production Rates ◽  
Temporal And Spatial

Abstract. We developed a new method for the determination of dissolved nitric oxide (NO) in discrete seawater samples based on the combination of a purge-and-trap setup and a fluorometric detection of NO. 2,3-diaminonaphthalene (DAN) reacts with NO in seawater to form the highly fluorescent 2,3-naphthotriazole (NAT). The fluorescence intensity was linear for NO concentrations in the range from 0.14 to 19 nmol L−1. We determined a detection limit of 0.068 nmol L−1, an average recovery coefficient of 83.8 % (80.2–90.0 %), and a relative standard deviation of ±7.2 %. With our method we determined for the first time the temporal and spatial distributions of NO surface concentrations in coastal waters of the Yellow Sea off Qingdao and in Jiaozhou Bay during a cruise in November 2009. The concentrations of NO varied from below the detection limit to 0.50 nmol L−1 with an average of 0.26 ± 0.14 nmol L−1. NO surface concentrations were generally enhanced significantly during daytime, implying that NO formation processes such as NO2− photolysis are much higher during daytime than chemical NO consumption, which, in turn, lead to a significant decrease in NO concentrations during nighttime. In general, NO surface concentrations and measured NO production rates were higher compared to previously reported measurements. This might be caused by the high NO2− surface concentrations encountered during the cruise. Moreover, additional measurements of NO production rates implied that the occurrence of particles and a temperature increase can enhance NO production rates. With the method introduced here, we have a reliable and comparably easy to use method at hand to measure oceanic NO surface concentrations, which can be used to decipher both its temporal and spatial distributions as well as its biogeochemical pathways in the oceans.

Sources of nitric oxide during the outbreak of Ulva prolifera in coastal waters of the Yellow Sea off Qingdao

2020 ◽  
Vol 162 ◽  
pp. 105177
Author(s):  
Ke-Ke Wang ◽  
Ye Tian ◽  
Pei-Feng Li ◽  
Chun-Ying Liu ◽  
Gui-Peng Yang
Keyword(s):  
Nitric Oxide ◽  
Coastal Waters ◽  
Yellow Sea ◽  
Ulva Prolifera ◽  
The Yellow Sea

scholarly journals Nitric oxide (NO) in the Bohai Sea and the Yellow Sea

2019 ◽  
Vol 16 (22) ◽  
pp. 4485-4496 ◽  
Author(s):  
Ye Tian ◽  
Chao Xue ◽  
Chun-Ying Liu ◽  
Gui-Peng Yang ◽  
Pei-Feng Li ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Surface Layer ◽  
Yellow Sea ◽  
Bohai Sea ◽  
Limit Of Detection ◽  
Bottom Layer ◽  
The Yellow Sea ◽  
The Bohai Sea ◽  
Significant Difference ◽  
Average Flux

Abstract. Nitric oxide (NO) is a short-lived compound of the marine nitrogen cycle; however, our knowledge about its oceanic distribution and turnover is rudimentary. Here we present the measurements of dissolved NO in the surface and bottom layers at 75 stations in the Bohai Sea (BS) and the Yellow Sea (YS) in June 2011. Moreover, NO photoproduction rates were determined at 27 stations in both seas. The NO concentrations in the surface and bottom layers were highly variable and ranged from below the limit of detection (i.e., 32 pmol L−1) to 616 pmol L−1 in the surface layer and 482 pmol L−1 in the bottom layer. There was no significant difference (p>0.05) between the mean NO concentrations in the surface (186±108 pmol L−1) and bottom (174±123 pmol L−1) layers. A decreasing trend of NO in bottom-layer concentrations with salinity indicates a NO input by submarine groundwater discharge. NO in the surface layer was supersaturated at all stations during both day and night and therefore the BS and YS were a persistent source of NO to the atmosphere at the time of our measurements. The average flux was about 4.5×10-16 mol cm−2 s−1 and the flux showed significant positive relationship with the wind speed. The accumulation of NO during daytime was a result of photochemical production, and photoproduction rates were correlated to illuminance. The persistent nighttime NO supersaturation pointed to an unidentified NO dark production. NO sea-to-air flux densities were much lower than the NO photoproduction rates. Therefore, we conclude that the bulk of the NO produced in the mixed layer was rapidly consumed before its release to the atmosphere.

Nitric oxide production and absorption in trachea, bronchi, bronchioles, and respiratory bronchioles of humans

1999 ◽  
Vol 86 (1) ◽  
pp. 159-167 ◽  
Author(s):  
Arthur B. DuBois ◽  
Patrick M. Kelley ◽  
James S. Douglas ◽  
Vahid Mohsenin
Keyword(s):  
Nitric Oxide ◽  
Breath Holding ◽  
No Production ◽  
Production Rates ◽  
Oral Production ◽  
Clean Air ◽  
Equilibrium Concentrations ◽  
The Mean ◽  
Young Subjects ◽  
No Absorption

Different volumes of dead-space gas were collected and analyzed for nitric oxide (NO) content, either immediately after inspiration or after a period of breath holding on clean air or NO mixtures. This allowed calculation of NO equilibrium, NO production, and NO absorption. In seven young, healthy, adult nonsmokers, the mean NO equilibrium values in parts per billion (ppb) were 56 ± 11 (SE) in the trachea, 37 ± 6 in the bronchi, 21 ± 3 in the bronchioles, and 16 ± 2 in the respiratory bronchioles. At any given NO concentration, the NO absorption rate (in nl/min) equaled the NO concentration (in ppb) times A (the absorption coefficient in l/min). A values (in l/min) were 0.11 ± 0.01 in the trachea, 0.17 ± 0.04 in the bronchi, 0.66 ± 0.09 in the bronchioles, and 1.35 ± 0.32 in the respiratory bronchioles. NO equilibrium concentrations and production rates in one 74-yr-old subject were three to five times as high as those found in the young subjects. Mouth equilibrium NO concentrations were 3 and 6 parts per million in two subjects who had oral production rates of 6 and 23 nl/min, respectively. In conclusion, production and absorption of NO occur throughout the first 450 ml of the airways.

Vertical dynamics in community functioning of biofilm-dwelling ciliates during the colonisation process in coastal waters of the Yellow Sea

2019 ◽  
Vol 70 (11) ◽  
pp. 1611 ◽  
Author(s):  
Xiaoyun Bai ◽  
Congcong Guo ◽  
Mamun Abdullah Al ◽  
Alan Warren ◽  
Henglong Xu
Keyword(s):  
Functional Diversity ◽  
Functional Traits ◽  
Coastal Waters ◽  
Yellow Sea ◽  
Northern China ◽  
Diversity Indices ◽  
Functional Trait ◽  
The Yellow Sea ◽  
Biological Traits ◽  
Trait Distribution

Multifunctional trait analysis is increasingly recognised as an effective tool for assessing ecosystem function and environmental quality. Here, a baseline study was performed at four depths (i.e. 1, 2, 3.5 and 5m) in Yellow Sea coastal waters of northern China in order to determine the optimal depth for bioassessment using biological traits of biofilm-dwelling ciliates. Community-weighted means (CWM) from functional traits system were used to summarise the trait distribution and functional diversity of ciliates among the four depths during a 1-month colonisation period. Functional trait distribution revealed a clear temporal variation among the four depths. In total, 3 of 17 functional traits (i.e. feeding type, body size and flexibility) showed significant temporal patterns. Bootstrapped averaging and permutational multivariate analysis of variance (PERMANOVA) tests demonstrated that the colonisation pattern of biofilm-dwelling ciliates as expressed by CWM at 1 and 2m differed significantly from those at 3.5 and 5m. Functional diversity indices showed lower variability at 1 and 2m than at 3.5 and 5m. These results suggest that 1 and 2m are the preferred sampling depths for bioassessment of marine water quality using biological traits of biofilm-dwelling ciliates.

Picomol Assay of Nitric Oxide Screening in Biological Samples by Derivatization Combined with High-Throughput Microplate Format

2013 ◽  
Vol 423-426 ◽  
pp. 1786-1789
Author(s):  
Xiao Ling Zhang ◽  
Qiao Yang
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
High Throughput ◽  
Biological Samples ◽  
Biological Sample ◽  
Small Volume ◽  
Screening Method ◽  
Broad Variety ◽  
Sensitive Determination

Nitric oxide (NO) is a critical intra-and extra-cellular signaling molecule that mediates a broad variety of physiologic and pathophysiologic events, and has prompted rapid growth of investigations in investigating its physiology and generation mechanism of corresponding diseases. In this paper, a picomol assay of NO screening method using fluorescent derivatization combined with high-throughout microplate format has been developed and successfully applied to the determination of NO in biological samples. The satisfied detection limit and recovery of the proposed method demonstrates that it can be competent for the sensitive determination of NO in extremely small volume of biological sample.

scholarly journals A Rapid and Selective Method for Determining Potential Nitrosating Agents in Cosmetic Products by Chemiluminescence Detection of Nitric Oxide

1998 ◽  
Vol 81 (2) ◽  
pp. 368-372 ◽  
Author(s):  
Hardy J Chou ◽  
Ronald L Yates
Keyword(s):  
Nitric Oxide ◽  
Detection Limit ◽  
False Positive ◽  
Nitroso Compounds ◽  
Cosmetic Products ◽  
Chemiluminescence Detection ◽  
Selective Method ◽  
Selective Determination ◽  
Chemiluminescent Reaction

abstract A method was developed for rapid and selective determination of potential nitrosating agents at the part-per-billion level in cosmetic products. These compounds are chemically reduced to nitric oxide, which is determined by its chemiluminescent reaction with ozone. Suspended materials and colors in cosmetic products do not interfere. Hence their removal before analysis is not required. A detection limit of 33 ppb, calculated as nitrite, was obtained. No false-positive interferences were observed from antifoaming agents, several AZ-nitroso compounds, and nitrate up to 20 ppm. Among cosmetic products surveyed, potential nitrosating agents were found at levels ranging from 113 to 5021 ppb. No consistent relationship was found between levels of potential nitrosating agents and N-nitrosamines in the same products. However, the highest levels of nitrosating agents were most often associated with the highest levels of N-nitrosamines known to be present in the products.

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