Contribution of Sulfate-Reducing Bacteria to the Electricity Generation in Microbial Fuel Cells

2014 ◽  
Vol 1008-1009 ◽  
pp. 285-289 ◽  
Author(s):  
Chong Yang Gao ◽  
Ai Jie Wang ◽  
Yang Guo Zhao
Keyword(s):  
Fuel Cells ◽  
Power Density ◽  
Microbial Fuel Cells ◽  
Electricity Generation ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Microbial Community Analysis ◽  
Electricity Production ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Double-chambered microbial fuel cells (MFCs) were used to investigate the effect of sulfate and sulfate-reducing bacteria (SRB) on electricity generation by molybdate inhibition coupled with PCR-DGGE technique. Results showed that low influent sulfate (< 1470 mg/L) improved power density and voltage, while higher sulfate blocked the MFC efficiency. Molybdate inhibited the activity of SRB and consequently decreased MFC voltage and power density which confirmed some SRB were involved in the electricity generation. Microbial community analysis indicated thatDesulfovibrio desulfuricanscontributed to the electricity production and stability of MFC.

Mixed sulfate-reducing bacteria-enriched microbial fuel cells for the treatment of wastewater containing copper

Chemosphere ◽  
2017 ◽  
Vol 189 ◽  
pp. 134-142 ◽  
Author(s):  
Waheed Miran ◽  
Jiseon Jang ◽  
Mohsin Nawaz ◽  
Asif Shahzad ◽  
Sang Eun Jeong ◽  
...  
Keyword(s):  
Fuel Cells ◽  
Microbial Fuel Cells ◽  
Sulfate Reducing Bacteria ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Biosynthesized Iron Sulfide Nanocluster Enhanced Anodic Current Generation by Sulfate Reducing Bacteria in Microbial Fuel Cells

2018 ◽  
Vol 5 (24) ◽  
pp. 4015-4020 ◽  
Author(s):  
Muralidharan Murugan ◽  
Waheed Miran ◽  
Takuya Masuda ◽  
Dae S. Lee ◽  
Akihiro Okamoto
Keyword(s):  
Fuel Cells ◽  
Microbial Fuel Cells ◽  
Iron Sulfide ◽  
Sulfate Reducing Bacteria ◽  
Anodic Current ◽  
Current Generation ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Experimental Investigation on the Active Range of Sulfate-Reducing Bacteria for Geological Disposal

1994 ◽  
Vol 353 ◽  
Author(s):  
S. Fukunaga ◽  
H. Yoshikawa ◽  
K. Fujiki ◽  
H. Asano
Keyword(s):  
Experimental Investigation ◽  
Environmental Conditions ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Geological Disposal ◽  
Sulfate Reducing ◽  
Buffer Material ◽  
Ph Range ◽  
High Level ◽  
Reducing Bacteria

AbstractThe active range ofDesulfovibrio desulfuricans. a species of sulfate-reducing bacteria, was examined in terms of pH and Eh using a fermenter at controlled pH and Eh. Such research is important because sulfate-reducing bacteria (SRB) are thought to exist underground at depths equal to those of supposed repositories for high-level radioactive wastes and to be capable of inducing corrosion of the metals used in containment vessels.SRB activity was estimated at 35°C, with lactate as an electron donor, at a pH range from 7 to 11 and Eh range from 0 to -380 mV. Activity increased as pH approached neutral and Eh declined. The upper pH limit for activity was between 9.9 and 10.3, at Eh of -360 to -384 mV. The upper Eh limit for activity was between -68 and -3 mV, at pH 7.1. These results show that SRB can be made active at higher pH by decreasing Eh, and that the higher pH levels of 8 to 10 produced by use of the buffer material bentonite does not suppress SRB completely.A chart was obtained showing the active range ofDesulfovibrio desulfuricansin terms of pH and Eh. Such charts can be used to estimate the viability of SRB and other microorganisms when the environmental conditions of a repository are specified.

A comparison of the electrophoretic properties of the ATP-sulfurylases, APS-reductases, and sulfite reductases from cultures of dissimilatory sulfate-reducing bacteria

1973 ◽  
Vol 19 (3) ◽  
pp. 375-380 ◽  
Author(s):  
G. W. Skyring ◽  
P. A. Trudinger
Keyword(s):  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Disc Electrophoresis ◽  
Sulfite Reductase ◽  
Electrophoretic Variation ◽  
Sulfate Reducing ◽  
Aps Reductase ◽  
Reducing Bacteria ◽  
Atp Sulfurylases

ATP-sulfurylases, APS-reductases, and sulfite reductases (SO3−2 → S−2) have been detected by gel disc electrophoresis in 13 cultures of dissimilatory sulfate-reducing bacteria and their electrophoretic properties have been compared. With respect to these three enzymes only, the results were indicative of some interspecies and intergenus homologies. In the Desulfovibrio strains (except Desulfovibrio desulfuricans 8301 which does not contain desulfoviridin), the major sulfite reductase was electrophoretically coincident with desulfoviridin and, in the Desulfotomaculum strains, with a brown protein. Some distinct patterns of electrophoretically distinguishable forms of APS-reductase were found. Considerable electrophoretic variation was found among the ATP-sulfurylases.

Microbial diversity and population dynamics of activated sludge microbial communities participating in electricity generation in microbial fuel cells

2008 ◽  
Vol 58 (11) ◽  
pp. 2195-2201 ◽  
Author(s):  
D. Ki ◽  
J. Park ◽  
J. Lee ◽  
K. Yoo
Keyword(s):  
Fuel Cells ◽  
Activated Sludge ◽  
Microbial Diversity ◽  
Microbial Fuel Cells ◽  
Electricity Generation ◽  
Municipal Wastewater ◽  
Treatment Plant ◽  
Community Analysis ◽  
Electricity Production ◽  
Anode Chamber

In this study, we performed microbial community analysis to examine microbial diversity and community structure in microbial fuel cells (MFCs) seeded with activated sludge from a municipal wastewater treatment plant in South Korea. Because anode-attached biofilm populations are particularly important in electricity transfer, the ecological characteristics of anode-attached biofilm microbes were explored and compared with those of microbes grown in suspension in an anode chamber. 16S rDNA-based community analysis showed that the degree of diversity in anode-attached biofilms was greater than that of the originally seeded activated sludge as well as that of the suspension-grown microbes in the anode bottle. In addition, Bacteroidetes and Clostridia grew preferentially during MFC electricity generation. Further phylogenetic analysis revealed that the anode biofilm populations described in this work are phylogenetically distant from previously characterized MFC anode biofilm microbes. These findings suggest that a phylogenetically diverse set of microbes can be involved in the electricity generation of MFC anode compartments, and that increased microbial diversity in anode biofilms may help to stabilize electricity production in the MFC.

Electricity Production in Microbial Fuel Cells Using Brush Bio-Anode and Bio-Cathode Made of PAN-Based Carbon Fibers

2012 ◽  
Vol 217-219 ◽  
pp. 956-960 ◽  
Author(s):  
En Ren Zhang ◽  
Qiang Ji ◽  
Lei Liu
Keyword(s):  
Fuel Cells ◽  
Electrochemical Properties ◽  
Power Density ◽  
Carbon Fibers ◽  
Microbial Fuel Cells ◽  
Continuous Flow ◽  
Electrode Materials ◽  
Electricity Production ◽  
Flow Mode ◽  
Bacterial Cultures

Microbial fuel cells with brush bio-anode and bio-cathode made of PAN-based carbon fibers were constructed, and the electricity production was investigated. Experimental results indicate that both the anode and the cathode could be catalyzed by mixed bacterial cultures. Oxygen-reduction at the cathode could be carried out effectively with the assistance of catalytic action by bacteria, enhancing the electrochemical properties of the cathode. Stable electricity production could be obtained with maximum power 5.6 mW (corresponding power density ~2.1 W/m3 MFC volume) when operating MFC in continuous flow mode. PAN-based carbon fibers were shown to be suitable electrode materials for MFCs, especially in systems for the future applications.

Response of Desulfovibrio desulfuricans colonies to oxygen stress

1990 ◽  
Vol 36 (6) ◽  
pp. 400-408 ◽  
Author(s):  
Judy D. Wall ◽  
Barbara J. Rapp-Giles ◽  
Merton F. Brown ◽  
Jerry A. White
Keyword(s):  
Electron Microscopy ◽  
Morphological Changes ◽  
Metal Sulfide ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Strictly Anaerobic ◽  
Air Oxidation ◽  
Oxygen Tolerance ◽  
Sulfate Reducing ◽  
Reducing Bacteria

Oxygen tolerance of the strictly anaerobic sulfate-reducing bacteria is well documented and poorly understood. This capacity for surviving brief exposures to oxygen must be a major factor in the diversity of environmental niches observed for these bacteria. We observed that viable cells of Desulfovibrio desulfuricans (ATCC 27774) could be found in colonies on the surface of solidified medium exposed to air for periods as long as 1 month. During exposure to air, the originally black colonies became greyish white, presumably as a result of the air oxidation of the metal sulfide deposits. A black, brittle deposit formed at the bottom of the colony and, simultaneously, the colony descended into a dimple that developed into a well in the agar. Eventually the colony reached the bottom of the Petri dish. These changes did not take place when the colonies were maintained in an anaerobic chamber. The morphological changes took place with all strains tested: three strains of D. desulfuricans and one strain of Desulfovibrio gigas and Desulfovibrio multispirans. Continued sulfate reduction appeared to be essential. Cyclic sulfate (thiosulfate or sulfite) reduction to sulfide and reoxidation of sulfide by the oxygen in air are proposed to maintain the viability of the bacteria by providing substrates for energy production and by reducing oxygen tension. Scanning and transmission electron microscopy of colony and cellular changes are shown. Key words: Desulfovibrio, sulfate-reducing bacteria, oxygen tolerance, sulfate cycling, scanning electron microscopy.

scholarly journals Reduction of perchlorate ions by the sulfate-reducing bacteria Desulfotomaculum sp. and Desulfovibrio desulfuricans

2020 ◽  
Vol 11 (2) ◽  
pp. 278-282
Author(s):  
N. S. Verkholiak ◽  
T. B. Peretyatko ◽  
A. A. Halushka
Keyword(s):  
Sewage Treatment ◽  
Desulfovibrio Desulfuricans ◽  
Sulfate Reducing Bacteria ◽  
Inhibitory Effect ◽  
Electron Acceptors ◽  
Wood Chips ◽  
Sulfate Ions ◽  
Sulfate Reducing ◽  
Perchlorate Ion ◽  
Reducing Bacteria

The usage of microorganisms to clean the environment from xenobiotics, in particular chlorine-containing ones, is a promising method of detoxifying the contaminated environment. Sulfate-reducing bacteria Desulfovibrio desulfuricans Ya-11, isolated from Yavoriv Lake, and Desulfotomaculum AR1, isolated from the Lviv sewage treatment system, are able to grow under conditions of environmental contamination by aromatic compounds and chlorine-containing substances. Due to their high redox potential, chlorate and perchlorate ions can be ideal electron acceptors for the metabolism of microorganisms. To test the growth of the tested microorganisms under the influence of perchlorate ions, bacteria were cultured in modified Postgate C medium with ClO4–. Biomass was determined turbidimetrically, the content of sulfate ions and hydrogen sulfide – photoelectrocolorimetrically, the content of perchlorate ions – permanganatometrically. The study of the ability of sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 to grow in a medium with perchlorate ions as electron acceptors showed the inhibitory effect of ClO4– on sulfate ion reduction by bacteria. Bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 are able to grow in environments with aromatic hydrocarbons, in particular toluene. The possibility of the growth of sulfate-reducing bacteria in the presence of toluene as an electron donor and perchlorate ions as an electron acceptor was investigated. The efficiency of perchlorate ion utilization by sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 was about 90 %. The effect of molybdenum on the reduction of perchlorate ions by Desulfotomaculum AR1 is shown in the paper. Immobilization of bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 was carried out in 3% agar and on wood chips. The ability of bacteria, immobilized on these media, to purify the aqueous medium from perchlorate ions was investigated. Reduction of perchlorate ions is more efficiently performed by cells of Desulfotomaculum AR1 and D. desulfuricans Ya-11 bacteria immobilized in agar than on wood chips. Sulfate-reducing bacteria Desulfotomaculum AR1 and D. desulfuricans Ya-11 are able to use perchlorate ions as electron acceptors, purifying the polluted aquatic environment from these pollutants.

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