Metal Removal from Spent Catalyst Using Microbacterium liquefaciens in Solid Culture

2015 ◽  
Vol 1130 ◽  
pp. 564-567 ◽  
Author(s):  
Norma G. Rojas-Avelizapa ◽  
Marlenne Gomez-Ramirez ◽  
Alamilla Martínez Diana Grecia
Keyword(s):  
Metal Removal ◽  
Pulp Density ◽  
Microbial Culture ◽  
Solid Culture ◽  
Spent Catalyst ◽  
Column System ◽  
Silver Mine ◽  
Glass Column ◽  
Microbial Removal ◽  
Growth Ph

The purpose of the present study was to investigate the ability ofMicrobacterium liquefaciensstrain MNSH2-PHGII-2, isolated from a Mexican silver mine, for removing Ni and V from spent catalyst at 80% (w/v) pulp density in a glass-column system at laboratory conditions. Firstly, microbial culture was adapted to spent catalyst at 0.1% (w/v) in liquid culture then, it was assayed by its ability to remove Ni and V from a spent catalyst in a glass-column system. Spent catalyst was packed at 80% (w/v) pulp density and inoculated at 20% (3x108CFU/ml); air was supplied at 80 ml/min and then incubated at 30°C during 14 days. Parameters such as microbial growth, pH, Ni and V residual concentrations, in catalyst, were determined at days 7 and 14. The result showed thatMicrobacterium liquefaciensstrain MNSH2-PHGII-2 in the glass-column system was able to remove 1007.4 mg/kg of Ni while V was removed at an extent of 5360.5 mg/kg. Microbial removal for other metals in catalyst was non-significant, that indicated the specificity ofMicrobacterium liquefaciensto remove Ni and V.

Novel Filamentous Fungi for Metal Removal from Spent Catalyst

2015 ◽  
Vol 1130 ◽  
pp. 673-676 ◽  
Author(s):  
Marlenne Gómez-Ramírez ◽  
Abel Plata-González ◽  
Grisel Fierros-Romero ◽  
Norma G. Rojas-Avelizapa
Keyword(s):  
Organic Acids ◽  
Filamentous Fungi ◽  
Statistical Difference ◽  
Metal Removal ◽  
Culture Media ◽  
Pulp Density ◽  
Spent Catalyst

The present study evaluated the ability of organic acids (leaching agent) produced by three filamentous fungi coded as MNSS-AH-5, MV-AH-2 and MV-AH-1 obtained from tailings samples to remove Ni and V from spent catalyst at 16 % (w/v) pulp density. Organic acids were produced in 500 mL Erlenmeyer flasks containing 150 mL of sucrose media where flasks were inoculated with 10% (1x107 conidia/mL) of each fungus and incubated during 6 days at 30°C, 150 rpm. The leaching agent produced by MNSS-AH-5, MV-AH-2 and MV-AH-1 in sucrose media were assayed to remove Ni and V from spent catalyst at 16% (w/v) pulp density during 7 days at 30°C, 150 rpm. At the end of incubation, Ni and V content was evaluated in bio treated catalyst samples by ICP-EOS. Controls containing sucrose media were included in order to evaluate the action of culture media on leaching; all experiments were done in duplicated. Results showed that after 7 days of incubation, the organic acids produced by MNSS-AH-5 were able to leach 2525 mg/Kg of Ni corresponding to 21 %, not significance difference was observed for organic acids of MV-AH-2 and MV-AH-1. In the case of V, all bioleaching treatments presented statistical difference observing a V leaching of 10921.8, 11099.3 and 4757.5 mg/Kg corresponding to 23.5, 24 and 10% for MNSS-AH-5, MV-AH-2 and MV-AH-1 respectively. Isolate MNSS-AH-5 was identified as Acremonium sp., MV-AH-2 and MV-AH-1 were identified as belonging to genera Penicillium. Based on results, this study shows that leaching agent produced by fungi could be an excellent biological alternative for the removal of Ni and V spent catalyst.

scholarly journals Effect of biostimulation on biodegradation of dissolved organic carbon in biological filters

2012 ◽  
Vol 5 (1) ◽  
pp. 23-29
Author(s):  
K. Tihomirova ◽  
A. Briedis ◽  
J. Rubulis ◽  
T. Juhna
Keyword(s):  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Rate Constant ◽  
Strong Positive Correlation ◽  
Biodegradation Rate ◽  
Column System ◽  
Glass Column ◽  
Standard Solution ◽  
Biodegradable Dissolved Organic Carbon ◽  
Biological Filters

Abstract. The addition of labile organic carbon (LOC) to enhance the biodegradation rate of dissolved organic carbon (DOC) in biological columns was studied. Acetate standard solution (NaAc) and Luria Bertrani (LB) medium were used as LOC as biostimulants in glass column system used for measurements of biodegradable dissolved organic carbon (BDOC). The addition of LOC related with the increase of total DOC in sample. The concentration of BDOC increased up to 7 and 5 times and was utilized after 24 min. contact time. The biodegradation rate constant was increased at least 26 times during adaptation-biostimulation period. There was a strong positive correlation between the biodegradation rate constant and the concentration of BDOC. Biostimulation period ranged from 24 to 53 h for NaAc biostimulant and from 20 to 168 h for LB. The study has shown that LOC could be used as stimulator to enhance the biodegradation rate of DOC during biofiltration.

Bio-Hydrometallurgical Processing of Non-Ferrous Metals from Copper Smelting Slag

2013 ◽  
Vol 825 ◽  
pp. 250-253 ◽  
Author(s):  
Sadia Ilyas ◽  
Jae Chun Lee ◽  
Do Yun Shin ◽  
Byung Su Kim
Keyword(s):  
Pulp Density ◽  
Stirred Tank ◽  
Copper Smelting ◽  
Microbial Culture ◽  
Stirred Tank Reactor ◽  
Tank Reactor ◽  
Ferrous Metals ◽  
Leaching Experiments ◽  
Smelting Slag ◽  
Non Ferrous Metals

Stirred tank reactor (STR) leaching of non-ferrous metals from copper smelting slag was conducted using biogenic Fe3+solution containing H2SO4. The solution was initially obtained through biooxidation of FeS2+S° byusing a moderately thermophilic microbial culture such asSulfobacillus thermosulfidooxidansat 45°C and was applied as lixiviant in STR leaching experiments. The effect of pH, pulp density, temperature and initial Fe3+concentration on leaching behavior of Cu, Fe, Zn and Ni from the slag were investigated. The presence of high concentration of Fe3+ions was found to promote the leaching of copper and nickel while inhibiting the leaching of zinc and iron. Process pH was considered as the most significant parameter and the optimum leaching results were obtained at pH 1.8 with low fayalite dissolution and jarosite precipitation. During the leaching at this pH (1.8), 25% (w/v) pulp density and 65°C temperature in presence of 20 g/L initial Fe3+concentration, maximum extraction of Cu (90%) and Ni (85%) was observed in 4 hours whereas Fe extraction was minimum (19%). A schematic flowsheet of multistage leaching process was proposed. The result obtained from multistage batch leaching experiments will be step forward for continuous stirred tank reactor leaching for industrial scale implementation.

scholarly journals Effect of pulp density on the bioleaching of metals from petroleum refinery spent catalyst

3 Biotech ◽  
2021 ◽  
Vol 11 (3) ◽  
Author(s):  
Neha Nagar ◽  
Himanshi Garg ◽  
Neha Sharma ◽  
Samuel Ayowole Awe ◽  
Chandra Sekhar Gahan
Keyword(s):  
Petroleum Refinery ◽  
Pulp Density ◽  
Spent Catalyst

Diverse molecular resistance mechanisms of Bacillus megaterium during metal removal present in a spent catalyst

2017 ◽  
Vol 22 (3) ◽  
pp. 296-307 ◽  
Author(s):  
Andrea Rivas-Castillo ◽  
Domancar Orona-Tamayo ◽  
Marlenne Gómez-Ramírez ◽  
Norma G. Rojas-Avelizapa
Keyword(s):  
Bacillus Megaterium ◽  
Metal Removal ◽  
Resistance Mechanisms ◽  
Spent Catalyst

Bioleaching of Valuable Metals from Spent Catalyst Using Metabolic Citiric Acid by Aspergillus niger

2020 ◽  
Vol 898 ◽  
pp. 23-28
Author(s):  
Himawan Tri Bayu Murti Petrus ◽  
Hotden Manurung ◽  
Rivky Juarsa Aditya ◽  
Rifani Amanda ◽  
Widi Astuti
Keyword(s):  
Particle Size ◽  
Aspergillus Niger ◽  
Metal Recovery ◽  
Pulp Density ◽  
Spent Catalyst ◽  
Valuable Metal ◽  
Weak Organic Acid ◽  
Valuable Metals ◽  
Calcium Iron ◽  
Leaching Efficiency

Spent catalyst is listed as one of the hazardous wastes. Based on the toxicity characteristic shows that spent catalyst contains some heavy metals at concentration above the regulations limits. This situattion becomes an important issue in nowadays. In this research, fungus Aspergillus niger was investigated to produce weak organic acid (citric acid). Batch experiments were performed to compare the leaching efficiency from spent catalyst of pulp density (2% and 4%) and particle size (212 µm, 150 µm and 75 µm). Result showed that after direct bioleaching process, maximum recovery of valuable metal 24.94%, 7.42%, 1.09%, 3.51%, 4.87% and 1.66% were achieved for aluminum, calcium, iron, copper, silver and platinum respectively at 2% pulp density. Overall data shows that metal recovery at pulp density 2% are higher than 4% pulp density. The maximum recovery based on particle size shows that the smaller particle (75 µm) the higher metal recovery (Al, Ag and Pt) and for some metals was different such as Fe, Cu and Ca.

scholarly journals Metal leaching from a spent catalyst by Alternaria alternata

2017 ◽  
Vol 2 (2) ◽  
pp. 221-231 ◽  
Author(s):  
Norma G. Rojas-Avelizapa ◽  
Jessica Otamendi-Valdez ◽  
Marlenne Gómez-Ramírez
Keyword(s):  
Alternaria Alternata ◽  
Culture Medium ◽  
Catalyst Concentration ◽  
Metal Removal ◽  
Culture Media ◽  
Hazardous Wastes ◽  
Spent Catalyst ◽  
Extracellular Medium ◽  
New Research ◽  
Selection Of

The study evaluated the leaching ability of the extracellular medium produced by a fungus, which was identified as Alternaria alternata. The study included the selection of a culture medium that favored the organic acid and siderophore production; sucrose and Czapek media were evaluated. Once the culture media was selected, bioleaching study of the catalyst at 1 and 5% continued, using only the extracellular media incubating at 30° C, 150 rpm during 7 days. Results indicated that the extracellular media produced by A. alternata in sucrose media decreases pH from 6 to 4.8; in this media siderophores were also produced. The study of metal bioleaching, using the extracellular media, showed that metals were leached at different extent, and it was observed that catalyst concentration had an important effect on the metal removal and on the order of the removal. It was found that at 1% catalyst and after 48 h of treatment, the following metals were removed, in descending order, V> Al> Si> Mo > Mg> Fe> Ni> As> Cr. This is the first report on the metal removal from the catalyst by the extracellular media of A. alternata which opens new research alternatives for the treatment of hazardous wastes with high metallic content.

Metal removal and morphological changes of B. megaterium in the presence of a spent catalyst

2019 ◽  
Vol 54 (6) ◽  
pp. 543-550 ◽  
Author(s):  
Andrea M. Rivas-Castillo ◽  
María E. Guatemala-Cisneros ◽  
Marlenne Gómez-Ramírez ◽  
Norma G. Rojas-Avelizapa
Keyword(s):  
Metal Removal ◽  
Morphological Changes ◽  
Spent Catalyst

scholarly journals Effect of biostimulation on biodegradation of dissolved organic carbon in biological granular activated carbon filters

2012 ◽  
Vol 5 (1) ◽  
pp. 67-83
Author(s):  
K. Tihomirova ◽  
A. Briedis ◽  
J. Rubulis ◽  
T. Juhna
Keyword(s):  
Organic Carbon ◽  
Dissolved Organic Carbon ◽  
Rate Constant ◽  
Contact Time ◽  
Strong Positive Correlation ◽  
Biodegradation Rate ◽  
Column System ◽  
Glass Column ◽  
Standard Solution ◽  
Biodegradable Dissolved Organic Carbon

Abstract. The addition of labile organic carbon (LOC) to enhance the biodegradation rate of dissolved organic carbon (DOC) in biological columns was studied. Acetate standard solution (NaAc) and LB (Luria Bertrani) medium were used as LOC as biostimulants in glass column system used for measurements of biodegradable dissolved organic carbon (BDOC). The addition of LOC related with the increase of total DOC in sample. The concentration of BDOC increased up to 7 and 5 times and was utilized after 24 min. contact time. The biodegradation rate constant was increased at least 8 times during adaptation-biostimulation period. There was a strong positive correlation between the biodegradation rate constant and the concentration of BDOC. Biostimulation period ranged from 24 to 53 h for NaAc biostimulant and from 20 to 168 h for LB. The study has shown that LOC could be used as stimulator to enhance the biodegradation rate of DOC during biofiltration.

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