Fixed Speed Release System: Huperzine a Loaded Microspheres in Thermosensitive Methylcellulose Based Hydrogel

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.236-238.2491 ◽

2011 ◽

Vol 236-238 ◽

pp. 2491-2494

Author(s):

Rui Sun ◽

Si Hao Chen ◽

Chen Chen Xing

Keyword(s):

In Vitro Release ◽

Huperzine A ◽

Injection System ◽

Release System ◽

In Situ Hydrogel ◽

The Difference ◽

Vitro Release

Huperzine A loaded microspheres are prepared using a W/O solvent evaporation method. The fixed speed release system is that microspheres composed with thermosensitive methylcellulose based hydrogel. SEM is used to analysis the surface morphology of Huperzine A loaded microspheres, which is shown that the difference of the size of microspheres. The size less than 200µm can be used as injection. Gelation experiment shows that the methylcellulose based solution can be transformed into in-situ hydrogel in 15 minutes at body temperature. The result of in vitro release shows that Huperzine A released in a nearly fixed speed. All the meterials of experiment can be biodegrated completely without operating to remove the gel. This is a promising long-term injection system, which is security and controlable for clinic.

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Study of the interaction between self-assembling peptide and mangiferin and in vitro release of mangiferin from in situ hydrogel

International Journal of Nanomedicine ◽

10.2147/ijn.s208267 ◽

2019 ◽

Vol Volume 14 ◽

pp. 7447-7460 ◽

Cited By ~ 1

Author(s):

Cui Meng ◽

Weipeng Wei ◽

Yuhe Wang ◽

Kunqin Zhang ◽

Ting Zhang ◽

...

Keyword(s):

In Vitro Release ◽

Self Assembling ◽

In Situ Hydrogel ◽

Vitro Release

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In situ fiber optic method for long-term in vitro release testing of microspheres

International Journal of Pharmaceutics ◽

10.1016/j.ijpharm.2008.01.017 ◽

2008 ◽

Vol 356 (1-2) ◽

pp. 206-211 ◽

Cited By ~ 17

Author(s):

Jennifer M. Voisine ◽

Banu S. Zolnik ◽

Diane J. Burgess

Keyword(s):

Fiber Optic ◽

In Vitro Release ◽

Vitro Release ◽

Release Testing

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Reliability of a Single β-Thromboglobulin Measurement in a Diabetic Population : Importance of PGE1 in Anticoagulant Mixture

Thrombosis and Haemostasis ◽

10.1055/s-0038-1651094 ◽

1987 ◽

Vol 57 (02) ◽

pp. 201-204 ◽

Cited By ~ 2

Author(s):

P Y Scarabin ◽

L Strain ◽

C A Ludlam ◽

J Jones ◽

E M Kohner

Keyword(s):

In Vitro Release ◽

Mean Value ◽

Reliable Estimate ◽

Diabetic Patients ◽

Single Measurement ◽

Diabetic Population ◽

The Difference ◽

Vitro Release

SummaryDuring the collection of samples for plasma β-thromboglobulin (β-TG) determination, it is well established that artificially high values can be observed due to in-vitro release. To estimate the reliability of a single β-TG measurement, blood samples were collected simultaneously from both arms on two separate occasions in 56 diabetic patients selected for a clinical trial. From each arm, blood was taken into two tubes containing an anticoagulant mixture with (tube A) and without (tube B) PGE!. The overall mean value of B-TG in tube B was 1.14 times higher than in tube A (p <0.01). The markedly large between-arms variation accounted for the most part of within-subject variation in both tubes and was significantly greater in tube B than in tube A. Based on the difference between B-TG values from both arms, the number of subjects with artifically high B-TG values was significantly higher in tube B than in tube A on each occasion (overall rate: 28% and 14% respectively). Estimate of between-occasions variation showed that B-TG levels were relatively stable for each subject between two occasions in each tube. It is concluded that the use of PGEi decreases falsely high B-TG levels, but a single measurement of B-TG does not provide a reliable estimate of the true B-TG value in vivo.

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DEVELOPMENT AND INVESTIGATION OF TIMOLOL MALEATE AND TRAVOPROST COMBINATION NIOSOMAL IN SITU GELLING SYSTEM FOR THE TREATMENT OF GLAUCOMA

INDIAN DRUGS ◽

10.53879/id.52.07.10193 ◽

2015 ◽

Vol 52 (07) ◽

pp. 33-35

Author(s):

A Dubey ◽

◽

P Prabhu ◽

N Nair ◽

K Beladiya ◽

...

Keyword(s):

In Vitro Release ◽

Entrapment Efficiency ◽

Timolol Maleate ◽

In Situ Gel ◽

Vesicle Size ◽

Gelling Time ◽

In Situ Gelling ◽

Vitro Release

The aim of the present investigation was to develop a combination of timolol maleate and travoprost niosomal in situ gelling system for the treatment of glaucoma. Niosomes were prepared by thin film hydration technique using rotary flash evaporator. A 32 factorial design was utilized to study the effect of the molar ratio of Span 60 (X1) and cholesterol (X2) on vesicle size, drug entrapment efficiency and in vitro release study. On the basis of vesicle size, maximum entrapment efficiency and in vitro release of drug, best formulations were selected for the preparation of niosomal in situ gel (Drop). On the basis of gelling time and viscosity, optimized ratio of the polymers was selected for the desired preparation. Selected niosomal batches were dispersed in carbopol 940 and HPMC K4M polymer solution (combination IF6) to form in situ gel niosomal formulations (Drop). The gelling time of the niosomal in situ gel (NIF1) was found to be the best (+++) and the viscosity was found to be 1190 cP. Zeta potential, average size analysis, polydispersibility index value was found to be -45.1 mV, 256.5 nm, 0.228 respectively. In vitro drug release was found to be within the range of 50.23 ± 0.54 to 60.23 ± 0.33% over the period of 6 h. IOP lowering activity of best formulation (NIF1) showed more significant and sustained effect than the marketed eye drops. Best formulation (NIF1) was found to be stable, sterile, non irritant and isotonic. Hence niosomal in situ gelling combination system may have the potential of bringing better application than the conventional ocular therapy with improved ocular bioavailability and increased patient compliance.

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Preparation of a Sustained Release Drug Delivery System for Dexamethasone by a Thermosensitive, In Situ Forming Hydrogel for Use in Differentiation of Dental Pulp

ISRN Pharmaceutics ◽

10.1155/2013/983053 ◽

2013 ◽

Vol 2013 ◽

pp. 1-6 ◽

Cited By ~ 1

Author(s):

Elham Khodaverdi ◽

Fatemeh Kheirandish ◽

Farnaz Sadat Mirzazadeh Tekie ◽

Bibi Zahra Khashyarmanesh ◽

Farzin Hadizadeh ◽

...

Keyword(s):

Phase Transition ◽

Transition Temperature ◽

Phase Transition Temperature ◽

Delivery System ◽

In Vitro Release ◽

In Situ Forming ◽

Release Study ◽

Vitro Release

In situ forming delivery systems composed of block copolymers are attracting substantial attention due to their ease of use, biocompatibility, and biodegradability. In this study, the thermoresponsive triblock copolymer PLGA-PEG-PLGA was studied as a dexamethasone delivery system. Dexamethasone, a synthetic glucocorticoid, is used clinically to improve inflammation, pain, and the hyperemesis of chemotherapy, and it is applied experimentally as a differentiation factor in tissue engineering. PLGA-PEG-PLGA was synthesised under microwave irradiation for 5 min. The obtained copolymer was characterised to determine its structure and phase transition temperature. An in vitro release study was conducted for various copolymer structures and drug concentrations. The yield of the reaction and HNMR analysis confirmed the appropriateness of the microwave-assisted method for PLGA-PEG-PLGA synthesis. Phase transition temperature was affected by the drug molecule as well as by the copolymer concentration and structure. An in vitro release study demonstrated that release occurs mainly by diffusion and does not depend on the copolymer structure or dexamethasone concentration.

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