Repair of Rabbit Facial Nerve with Amniotic Extracellular Matrix Based on Biological Material

2011 ◽  
Vol 322 ◽  
pp. 169-172
Author(s):  
Zhen Gao ◽  
Xiao Ting Luo ◽  
Nian Sheng Li ◽  
Wei Deng ◽  
Shu Mei Li
Keyword(s):  
Extracellular Matrix ◽  
Facial Nerve ◽  
Nerve Conduction ◽  
Peripheral Nerves ◽  
Nerve Conduction Velocity ◽  
Nerve Fibers ◽  
Nerve Graft ◽  
Nerve Conduit ◽  
Nerve Grafts ◽  
Facial Nerves

Objective: To evaluate the effects of amniotic extracellular matrix (AECM) on the repairation of facial nerve in rabbits. Methods: The transected nerve ends of the facial nerves of rabbits were then repaired with the AECM nerve conduit or an autologous nerve graft. After 3 months, the animals’ neural conductive velocity were determined. The myelinated fibers across the specimen were counted with histological examination. Results: The total count of medullated nerve fibers varied significantly, but the nerve conduction velocity had no significance between the AECM grafts and the autologous nerve grafts. Conclusion: AECM could become the biocompatible material for repairing the peripheral nerves.

scholarly journals Conduction velocity of the rabbit facial nerve: a noninvasive functional evaluation

2003 ◽  
Vol 17 (2) ◽  
pp. 126-131 ◽  
Author(s):  
Belmiro Cavalcanti do Egito Vasconcelos ◽  
Cosme Gay Escoda ◽  
Ricardo José de Holanda Vasconcellos ◽  
Riedel Frota Sá Nogueira Neves
Keyword(s):  
Facial Nerve ◽  
Conduction Velocity ◽  
Nerve Conduction ◽  
Action Potentials ◽  
Functional Evaluation ◽  
Surface Electrodes ◽  
Nerve Grafts ◽  
Synthetic Materials ◽  
Facial Nerves ◽  
The Mean

The aim of this study was to evaluate standardized conduction velocity data for uninjured facial nerve and facial nerve repaired with autologous graft nerves and synthetic materials. An evaluation was made measuring the preoperative differences in the facial nerve conduction velocities on either side, and ascertaining the existence of a positive correlation between facial nerve conduction velocity and the number of axons regenerated postoperatively. In 17 rabbits, bilateral facial nerve motor action potentials were recorded pre- and postoperatively. The stimulation surface electrodes were placed on the auricular pavilion (facial nerve trunk) and the recording surface electrodes were placed on the quadratus labii inferior muscle. The facial nerves were isolated, transected and separated 10 mm apart. The gap between the two nerve ends was repaired with autologous nerve grafts and PTFE-e (polytetrafluoroethylene) or collagen tubes. The mean of maximal conduction velocity of the facial nerve was 41.10 m/s. After 15 days no nerve conduction was evoked in the evaluated group. For the period of 2 and 4 months the mean conduction velocity was approximately 50% of the normal value in the subgroups assessed. A significant correlation was observed between the conduction velocity and the number of regenerated axons. Noninvasive functional evaluation with surface electrodes can be useful for stimulating and recording muscle action potentials and for assessing the functional state of the facial nerve.

Application of Amniotic Extracellular Matrix Nerve Conduit with Biological Material in the Peripheral Nerve Defect In Vivo

2011 ◽  
Vol 322 ◽  
pp. 173-176
Author(s):  
Zhen Gao ◽  
Xiao Ting Luo ◽  
Nian Sheng Li ◽  
Wei Deng ◽  
Shu Mei Li
Keyword(s):  
Extracellular Matrix ◽  
Peripheral Nerve ◽  
Facial Nerve ◽  
Silica Gel ◽  
Peripheral Nerve Regeneration ◽  
Nerve Fibers ◽  
Neural Regeneration ◽  
Nerve Conduit ◽  
Time Points

Objective: To evaluate the peripheral nerve regeneration using a nerve conduit of amniotic extracellular matrix (AECM). Methods: 5 mm gap in the rabbit facial nerve was repaired with AECM conduit or with a silica gel conduit. After 7 days, 1 month and 3 months, the medullated nerve fibers and neural conductive velocity were determined. Results: On the 7th day, no neural regeneration was observed. But the neoformative neural fibers across AECM and silica gel were seen in the following 3 months, while the number and conductive velocity of medullated nerve fibers varied significantly at the different time points. Conclusion: AECM could repair the peripheral nerve defect.

scholarly journals Nerve recovery from treatment with a vascularized nerve graft compared to an autologous non-vascularized nerve graft in animal models: A systematic review and meta-analysis

PLoS ONE ◽  
2021 ◽  
Vol 16 (12) ◽  
pp. e0252250
Author(s):  
Berend O. Broeren ◽  
Liron S. Duraku ◽  
Caroline A. Hundepool ◽  
Erik T. Walbeehm ◽  
J. Michiel Zuidam ◽  
...  
Keyword(s):  
Animal Models ◽  
Conduction Velocity ◽  
Nerve Conduction ◽  
Nerve Conduction Velocity ◽  
Meta Analysis ◽  
Nerve Graft ◽  
Muscle Weight ◽  
Nerve Grafts ◽  
Significant Difference ◽  
Nerve Recovery

Background Treatment of nerve injuries proves to be a worldwide clinical challenge. Vascularized nerve grafts are suggested to be a promising alternative for bridging a nerve gap to the current gold standard, an autologous non-vascularized nerve graft. However, there is no adequate clinical evidence for the beneficial effect of vascularized nerve grafts and they are still disputed in clinical practice. Objective To systematically review whether vascularized nerve grafts give a superior nerve recovery compared to non-vascularized nerve autografts regarding histological and electrophysiological outcomes in animal models. Material and methods PubMed and Embase were systematically searched. The inclusion criteria were as follows: 1) the study was an original full paper which presented unique data; 2) a clear comparison between a vascularized and a non-vascularized autologous nerve transfer was made; 3) the population study were animals of all genders and ages. A standardized mean difference and 95% confidence intervals for each comparison was calculated to estimate the overall effect. Subgroup analyses were conducted on graft length, species and time frames. Results Fourteen articles were included in this review and all were included in the meta-analyses. A vascularized nerve graft resulted in a significantly larger diameter, higher nerve conduction velocity and axonal count compared to an autologous non-vascularized nerve graft. However, during sensitivity analysis the effect on axonal count disappeared. No significant difference was observed in muscle weight. Conclusion Treating a nerve gap with a vascularized graft results in superior nerve recovery compared to non-vascularized nerve autografts in terms of axon count, diameter and nerve conduction velocity. No difference in muscle weight was seen. However, this conclusion needs to be taken with some caution due to the inherent limitations of this meta-analysis. We recommend future studies to be performed under conditions more closely resembling human circumstances and to use long nerve defects.

scholarly journals Muscle graft as a substitute for peripheral nerve graft in rats

2009 ◽  
Vol 24 (3) ◽  
pp. 221-225 ◽  
Author(s):  
Aristides Palhares ◽  
Fausto Viterbo ◽  
Ricardo Galesso Cardoso
Keyword(s):  
Peripheral Nerve ◽  
Nerve Injury ◽  
Nerve Fibers ◽  
Nerve Graft ◽  
Muscle Graft ◽  
Nerve Grafts ◽  
Peripheral Nerve Graft ◽  
Histological Appearance ◽  
Almost All

PURPOSE: To evaluate the applicability of the use of autogenous muscle treated in various ways, as a substitute of the nerve grafts. METHODS: Rats were divided into seven groups that received, as a treatment for a standard nerve injury, the following types of grafts: fresh muscle, muscle fixed with 10% formaldehyde, muscle frozen in a freezer, muscle frozen in refrigerator, nerveless muscle, peripheral nerve and a group was without any treatment. It assessed the histological appearance of the nerve fibers in the segment repaired. RESULTS: The evaluation of the segment nervous repaired showed nerve fibers through the graft in almost all groups, but the methodology employed has not adequately characterized the differences between the groups. CONCLUSION: This study showed the migration of nerves fibers through all grafts used.

scholarly journals Estimation of Intraoperative Stimulation Threshold of the Facial Nerve in Patients Undergoing Microvascular Decompression

2019 ◽  
Vol 80 (06) ◽  
pp. 599-603
Author(s):  
Rafey A. Feroze ◽  
Michael M. McDowell ◽  
Jeffrey Balzer ◽  
Donald J. Crammond ◽  
Partha Thirumala ◽  
...  
Keyword(s):  
Trigeminal Neuralgia ◽  
Facial Nerve ◽  
Microvascular Decompression ◽  
Nerve Conduction ◽  
Tumor Burden ◽  
Constant Current ◽  
Facial Weakness ◽  
Stimulation Threshold ◽  
Compound Muscle Action Potentials ◽  
Facial Nerves

Introduction Facial weakness can result from surgical manipulation of the facial nerve. Intraoperative neuromonitoring reduces functional impairment but no clear guidelines exist regarding interpretation of intraoperative electrophysiological results. Most studies describe subjects with facial nerves encumbered by tumors or those with various grades of facial nerve weakness. We sought to obtain the neurophysiological parameters and stimulation threshold following intraoperative facial nerve triggered electromyography (t-EMG) stimulation during microvascular decompression for trigeminal neuralgia to characterize the response of normal facial nerves via t-EMG. Methods Facial nerve t-EMG stimulation was performed in seven patients undergoing microvascular decompression for trigeminal neuralgia. Using constant current stimulation, single stimulation pulses of 0.025 to 0.2 mA intensity were applied to the proximal facial nerve. Compound muscle action potentials, duration to onset, and termination of t-EMG responses were recorded for the orbicularis oculi and mentalis muscles. Patients were evaluated for facial weakness following the surgical procedure. Results Quantifiable t-EMG responses were generated in response to all tested stimulation currents of 0.025, 0.05, 0.1, and 0.2 mA in both muscles, indicating effective nerve conduction. No patients developed facial weakness postoperatively. Conclusions The presence of t-EMG amplitudes in response to 0.025 mA suggests that facial nerve conduction can take place at lower stimulation intensities than previously reported in patients with tumor burden. Proximal facial nerve stimulation that yields responses with thresholds less than 0.05 mA may be a preferred reference baseline for surgical procedures within the cerebellopontine angle to prevent iatrogenic injury.

scholarly journals AGE-RELATED PRESERVATION OF MOTOR NERVE CONDUCTION VELOCITY IN NEURONAL MTORC1 KNOCKDOWN MICE

2019 ◽  
Vol 3 (Supplement_1) ◽  
pp. S107-S107
Author(s):  
Stacy A Hussong ◽  
Veronica Galvan
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Conduction Velocity ◽  
Dietary Restriction ◽  
Nerve Conduction ◽  
Peripheral Nerves ◽  
Nerve Conduction Velocity ◽  
Age Related ◽  
Mtorc1 Signaling ◽  
Effects Of Aging

Abstract With age, peripheral nerves undergo demyelination along with overall decrease in peripheral nerve conduction velocity in both sensory and motor nerves. Loss of innervation in muscles is thought to be a major factor in causing age-related sarcopenia including a decrease in muscle function. Dietary restriction attenuates the detrimental effects of aging in mice. Reduction of mTOR signaling is hypothesized to have overlapping mechanisms with dietary restriction. Furthermore, inhibition of mTOR via rapamycin treatment is known to extend lifespan in mice as well as improve peripheral nerve myelination. Therefore, I hypothesized that reducing mTORC1 signaling in neurons would be able to ameliorate the deleterious effects of aging in peripheral nerves. An overall decrease in nerve conduction velocity was observed in both tail sensory and sural nerves with age (15 vs. 30 months). In neuronal mTORC1 KD animals, there was an age-related preservation of both sural and sciatic nerve conduction. Rapamycin treatment produced similar effects with a trend towards increased sciatic nerve conduction velocity in rapamycin-treated wild-type mice at 19 months. The preserve sciatic nerve conduction velocity could be partially explained by preserved myelination. Neuronal mTORC1 knockdown animals had more myelin in the sciatic nerve at 30 mo. as compared to age-matched controls. Overall, these data indicate that mTORC1 signaling plays a role in the age-related decline in peripheral nerve myelination as well as nerve conduction velocity. Future therapeutics could utilize rapamycin or other rapalogs to combat the decline in peripheral nerve function associated with age and other diseases as well.

scholarly journals Schwannoma in the Upper Limbs

2013 ◽  
Vol 2013 ◽  
pp. 1-4 ◽  
Author(s):  
Chris Yuk Kwan Tang ◽  
Boris Fung ◽  
Margaret Fok ◽  
Janet Zhu
Keyword(s):  
Peripheral Nerves ◽  
Motor Nerve ◽  
Distal Region ◽  
Nerve Graft ◽  
Nerve Grafting ◽  
Nerve Grafts ◽  
Functional Deficit ◽  
Patient Consent ◽  
New System ◽  
Mixed Nerve

Schwannomas are the commonest tumours of peripheral nerves. Despite the classical description that schwannomas are well encapsulated and can be completely enucleated during excision, a portion of them have fascicular involvement and could not be completely shelled out. A retrospective review for 8 patients was carried out over 10 years. 75% of schwannoma occurred over the distal region of upper limb (at elbow or distal to it). It occurs more in the mixed nerve instead of pure sensory or motor nerve. 50% of patients had mixed nerve involvement. Fascicular involvement was very common in schwannoma (75% of patients). Removal of the tumour with fascicles can cause functional deficit. At present, there is no method (including preoperative MRI) which can predict the occurrence of fascicular involvement; the authors therefore proposed a new system to stratify patients who may benefit from interfascicular nerve grafts. In this group of patients, the authors strongly recommend that the possibility and option of nerve graft should be discussed with patients prior to schwannoma excision, so that nerve grafting could be directly proceeded with patient consent in case there is fascicular involvement of tumour found intraoperatively.

Interface between the facial nerve and large acoustic neurinomas Immunohistochemical study of the cleavage plane in NF2 and non-NF2 cases

1994 ◽  
Vol 80 (3) ◽  
pp. 541-547 ◽  
Author(s):  
Juha Jääskeläinen ◽  
Anders Paetau ◽  
Ilmari Pyykkö ◽  
Göran Blomstedt ◽  
Tauno Palva ◽  
...  
Keyword(s):  
Facial Nerve ◽  
Cleavage Plane ◽  
Complete Removal ◽  
Nerve Fibers ◽  
Acoustic Neurinoma ◽  
Content Type ◽  
Clear Cut ◽  
Nerve Tumor ◽  
Facial Nerves ◽  
Acoustic Neurinomas

✓ In acoustic neurinoma surgery, the surgeon is required to find a cleavage plane between the facial nerve and the tumor, and with the aid of the operating microscope this is usually achieved by fine dissection. A histological specimen of the nerve-tumor interface is available only if the facial nerve was hopelessly adherent to the tumor (usually a large or giant neoplasm) and the surgeon decided to sever the nerve to obtain a complete removal. The authors have examined immunohistochemically the nerve-tumor interface of 20 such facial nerves (six cases of neurofibromatosis 2 (NF2) and 14 of non-NF2) in a series of 351 acoustic neurinomas. The largest extrameatal dimension of the 20 tumors ranged from 20 to 51 mm (median 39 mm). In all of these 20 instances the nerve-tumor contact area was at least partially devoid of a clear-cut histological cleavage plane. Where the facial nerve trunk was attached to the surface of the tumor, nerve fibers of the contact areas either abutted directly against tumor cells or nerve fibers were seen to penetrate into the tumor tissue. Frank embedding of nerve fibers was more frequent in NF2.

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