Application of Amniotic Extracellular Matrix Nerve Conduit with Biological Material in the Peripheral Nerve Defect In Vivo

2011 ◽  
Vol 322 ◽  
pp. 173-176
Author(s):  
Zhen Gao ◽  
Xiao Ting Luo ◽  
Nian Sheng Li ◽  
Wei Deng ◽  
Shu Mei Li
Keyword(s):  
Extracellular Matrix ◽  
Peripheral Nerve ◽  
Facial Nerve ◽  
Silica Gel ◽  
Peripheral Nerve Regeneration ◽  
Nerve Fibers ◽  
Neural Regeneration ◽  
Nerve Conduit ◽  
Time Points

Objective: To evaluate the peripheral nerve regeneration using a nerve conduit of amniotic extracellular matrix (AECM). Methods: 5 mm gap in the rabbit facial nerve was repaired with AECM conduit or with a silica gel conduit. After 7 days, 1 month and 3 months, the medullated nerve fibers and neural conductive velocity were determined. Results: On the 7th day, no neural regeneration was observed. But the neoformative neural fibers across AECM and silica gel were seen in the following 3 months, while the number and conductive velocity of medullated nerve fibers varied significantly at the different time points. Conclusion: AECM could repair the peripheral nerve defect.

scholarly journals Comparison of Decellularization Protocols to Generate Peripheral Nerve Grafts: A Study on Rat Sciatic Nerves

2021 ◽  
Vol 22 (5) ◽  
pp. 2389
Author(s):  
Marwa El El Soury ◽  
Óscar Darío García-García ◽  
Matteo Moretti ◽  
Isabelle Perroteau ◽  
Stefania Raimondo ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Peripheral Nerve ◽  
Peripheral Nerve Regeneration ◽  
Extracellular Matrix Component ◽  
Matrix Component ◽  
Viability Assay ◽  
In Vitro Characterization ◽  
Sciatic Nerves

In critical nerve gap repair, decellularized nerve allografts are considered a promising tissue engineering strategy that can provide superior regeneration results compared to nerve conduits. Decellularized nerves offer a well-conserved extracellular matrix component that has proven to play an important role in supporting axonal guiding and peripheral nerve regeneration. Up to now, the known decellularized techniques are time and effort consuming. The present study, performed on rat sciatic nerves, aims at investigating a novel nerve decellularization protocol able to combine an effective decellularization in short time with a good preservation of the extracellular matrix component. To do this, a decellularization protocol proven to be efficient for tendons (DN-P1) was compared with a decellularization protocol specifically developed for nerves (DN-P2). The outcomes of both the decellularization protocols were assessed by a series of in vitro evaluations, including qualitative and quantitative histological and immunohistochemical analyses, DNA quantification, SEM and TEM ultrastructural analyses, mechanical testing, and viability assay. The overall results showed that DN-P1 could provide promising results if tested in vivo, as the in vitro characterization demonstrated that DN-P1 conserved a better ultrastructure and ECM components compared to DN-P2. Most importantly, DN-P1 was shown to be highly biocompatible, supporting a greater number of viable metabolically active cells.

scholarly journals Long Non-coding RNA MSTRG.24008.1 Regulates the Regeneration of the Sciatic Nerve via the miR-331-3p–NLRP3/MAL Axis

2021 ◽  
Vol 9 ◽  
Author(s):  
Gang Yin ◽  
Ying Peng ◽  
Yaofa Lin ◽  
Peilin Wang ◽  
Zhuoxuan Li ◽  
...  
Keyword(s):  
Sciatic Nerve ◽  
Peripheral Nerve ◽  
Nerve Injury ◽  
Peripheral Nerve Regeneration ◽  
Clinical Problem ◽  
Neural Regeneration ◽  
Luciferase Reporter ◽  
Complex Biological Process

Peripheral nerve injury (PNI) is a common clinical problem, which can cause severe disability and dramatically affect a patient’s quality of life. Neural regeneration after PNI is a complex biological process that involves a variety of signaling pathways and genes. Emerging studies demonstrated that long non-coding RNAs (lncRNAs) were abnormally expressed after PNI and played pivotal roles in peripheral nerve regeneration. Based on the rat sciatic nerve injury model, we found that the expression levels of several lncRNAs were increased significantly in the sciatic nerve after injury. Software prediction prompted us to focus on one up-regulated lncRNA, MSTRG.24008.1. Dual-luciferase reporter assay, RNA pull-down assay and RNA interference approach verified that MSTRG.24008.1 regulated neuroregeneration via the miR-331-3p/nucleotide-binding oligomerization domain-like pyrin domain containing 3 (NLRP3)/myelin and lymphocyte protein (MAL) axis in vitro. Subsequently, we performed gastrocnemius muscle gravity and sciatic functional index experiments to evaluate the recovery of injured sciatic nerves after MSTRG.24008.1 siRNA interference in vivo. In conclusion, knockdown of MSTRG.24008.1 promotes the regeneration of the sciatic nerve via the miR-331-3p/NLRP3/MAL axis, which may provide a new strategy to evaluate and repair injured peripheral nerves clinically.

Repair of Rabbit Facial Nerve with Amniotic Extracellular Matrix Based on Biological Material

2011 ◽  
Vol 322 ◽  
pp. 169-172
Author(s):  
Zhen Gao ◽  
Xiao Ting Luo ◽  
Nian Sheng Li ◽  
Wei Deng ◽  
Shu Mei Li
Keyword(s):  
Extracellular Matrix ◽  
Facial Nerve ◽  
Nerve Conduction ◽  
Peripheral Nerves ◽  
Nerve Conduction Velocity ◽  
Nerve Fibers ◽  
Nerve Graft ◽  
Nerve Conduit ◽  
Nerve Grafts ◽  
Facial Nerves

Objective: To evaluate the effects of amniotic extracellular matrix (AECM) on the repairation of facial nerve in rabbits. Methods: The transected nerve ends of the facial nerves of rabbits were then repaired with the AECM nerve conduit or an autologous nerve graft. After 3 months, the animals’ neural conductive velocity were determined. The myelinated fibers across the specimen were counted with histological examination. Results: The total count of medullated nerve fibers varied significantly, but the nerve conduction velocity had no significance between the AECM grafts and the autologous nerve grafts. Conclusion: AECM could become the biocompatible material for repairing the peripheral nerves.

Functional Screening and Biosafety Identify of the Potential Application of Let-7a in Injured Peripheral Nerve Regeneration

2021 ◽  
Author(s):  
Qianqian Chen ◽  
Qianyan Liu ◽  
Pan Wang ◽  
Tianmei Qian ◽  
Xinghui Wang ◽  
...  
Keyword(s):  
Peripheral Nerve ◽  
Potential Application ◽  
Gene Expression Analysis ◽  
Nerve Repair ◽  
Peripheral Nerve Regeneration ◽  
Functional Approach ◽  
Cell Permeability ◽  
Functional Screening ◽  
The Core

Abstract Proper supporting factor can possess the ability to enhance neuron regeneration, for instance, neurotrophic effects especially nerve growth factor (NGF). However, the in vivo applications of NGF are largely limited by its intrinsic disadvantages. Considering that let-7 targets and regulates NGF, and let-7 is also the core and harbor regulators in peripheral nerve repair and regeneration, we evaluated the potential application in clinical. We firstly screened the let-7a as the most ideal let-7 family molecular by gene expression analysis and functional approach. We further evaluated the in vivo safety, the cell permeability of 3 main cells in regeneration micro-environment, and the morphological and functional indicators. Our study provides an essential basis for in vivo application of let-7 and pictured a vision for the clinical translation of miRNA as a prospective alternative for regenerative medicine.

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