Methanol Induced Changes in Structure and Properties of Dry Spinning Fibers of Regenerated Silk Fibroin

2011 ◽  
Vol 335-336 ◽  
pp. 908-911
Author(s):  
Yan Long Ma ◽  
Jing Xin Zhu ◽  
Hui Li Shao ◽  
Xue Chao Hu

The as-spun fibers, which be produced by dry spinning technique, were treated with methanol solution. The results showed that the morphology, structure and mechanical properties of the as-spun fibers had changed. Before methanol treatment,the as-spun fiber had a smooth surface, after methanol treatment, the fiber exhibited rough surface and banded features along the fiber axis. The structure of the as-spun fibers changed from random-coil or silk I conformation to silk II conformation and the breaking intensity of the as-spun fibers were improved after methanol treatment. Compared with degummed silk fibers, the fibers treated with methanol had higher module, but the breaking elongation was lower, the fibers were still brittle.

2012 ◽  
Vol 53 (6) ◽  
pp. 1154-1158 ◽  
Author(s):  
Wei-Luen Chen ◽  
Nai-Yen Fan ◽  
Jing-Wei Lee ◽  
Ruey-Mo Lin ◽  
Chien-Ping Ju ◽  
...  

2016 ◽  
Vol 8 (5) ◽  
pp. 3349-3358 ◽  
Author(s):  
Chao Zhang ◽  
Yaopeng Zhang ◽  
Huili Shao ◽  
Xuechao Hu

RSC Advances ◽  
2015 ◽  
Vol 5 (119) ◽  
pp. 98553-98558 ◽  
Author(s):  
Chen Liu ◽  
Jiaqi Sun ◽  
Min Shao ◽  
Bin Yang

Centrifugal spinning converts the conformation of silk fibroin from random coil to β-sheet more easily than electrospinning, which results in fiber differences on secondary structures, orientation and thermal properties.


2013 ◽  
Vol 28 (20) ◽  
pp. 2897-2902 ◽  
Author(s):  
Yuan Jin ◽  
Yaopeng Zhang ◽  
Yichun Hang ◽  
Huili Shao ◽  
Xuechao Hu

Abstract


2007 ◽  
Vol 342-343 ◽  
pp. 813-816 ◽  
Author(s):  
Lim Jeong ◽  
Kuen Yong Lee ◽  
Won Ho Park

Nonwoven nanofiber matrices were prepared by electrospinning a solution of silk fibroin (SF) dissolved either in formic acid or in 1,1,1,3,3,3-hexafluoro-2-isopropyl alcohol (HFIP). The mean diameter of the electrospun nanofibers prepared from SF dissolved in formic acid was 80 nm with a unimodal size distribution, which was smaller than those prepared from HFIP (380 nm). SF nanofibers were then treated with an aqueous methanol solution, and structural changes due to solvent-induced crystallization of SF were investigated using IR and 13C solid-state CP/MAS NMR spectroscopy. SF nanofibers prepared from formic acid were found to have a higher proportion of β-sheet conformations than those prepared from HFIP. Methanol treatment provided a fast and effective means to alter the secondary structure of both types of SF nanofibers from a random coil form to a β-sheet form. As demonstrated in the present study, this approach to controlling the dimensions and secondary structure of proteins using various solvents may be useful for the design and tailoring of materials for biomedical applications, especially for tissue engineering applications.


Polymers ◽  
2020 ◽  
Vol 12 (5) ◽  
pp. 1013 ◽  
Author(s):  
Hsuan-Chen Wu ◽  
Aditi Pandey ◽  
Liang-Yu Chang ◽  
Chieh-Yun Hsu ◽  
Thomas Chung-Kuang Yang ◽  
...  

The superlative mechanical properties of spider silk and its conspicuous variations have instigated significant interest over the past few years. However, current attempts to synthetically spin spider silk fibers often yield an inferior physical performance, owing to the improper molecular interactions of silk proteins. Considering this, herein, a post-treatment process to reorganize molecular structures and improve the physical strength of spider silk is reported. The major ampullate dragline silk from Nephila pilipes with a high β-sheet content and an adequate tensile strength was utilized as the study material, while that from Cyrtophora moluccensis was regarded as a reference. Our results indicated that the hydrothermal post-treatment (50–70 °C) of natural spider silk could effectively induce the alternation of secondary structures (random coil to β-sheet) and increase the overall tensile strength of the silk. Such advantageous post-treatment strategy when applied to regenerated spider silk also leads to an increment in the strength by ~2.5–3.0 folds, recapitulating ~90% of the strength of native spider silk. Overall, this study provides a facile and effective post-spinning means for enhancing the molecular structures and mechanical properties of as-spun silk threads, both natural and regenerated.


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