Sequencing Analysis of HA-A/A-MCO Process

2012 ◽  
Vol 548 ◽  
pp. 880-884
Author(s):  
Ning Zuo
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Phosphorus Removal ◽  
Bacterial Community Structure ◽  
Low Grade ◽  
Sequencing Analysis ◽  
Sludge Reduction ◽  
Bacillus Sp ◽  
Organic Matters ◽  
Phosphorous Removal

This study applies PCR-DGGE fingerprinting technique to analysis the bacterial community structure of phosphorous removal and denitrification system. Through strains identification, it is found that acinetobacter sp.is the base of keeping favorable phosphorus removal effect, Spirochaeta sp.undertakes the function of organic matters removal, Bacillus sp. and Lampropedia hyalina sp.can propagate largely in the germiculture area(No.1 oxic tank), and can be preyed on by highlevel microbe(such as protozoa and metazoan) in protozoa growing area(No.2oxic tank) and metazoan growimg area(No.3 oxic tank). The predation between highlevel microbe and low grade microbe can promote efficient sludge reduction of HA-A/A-MCO process.

Deep sequencing analysis of bacterial community structure of Soldhar hot spring, India

Microbiology ◽  
2017 ◽  
Vol 86 (1) ◽  
pp. 136-142 ◽  
Author(s):  
A. Sharma ◽  
D. Paul ◽  
D. Dhotre ◽  
K. Jani ◽  
A. Pandey ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Deep Sequencing ◽  
Bacterial Community Structure ◽  
Hot Spring ◽  
Sequencing Analysis ◽  
Deep Sequencing Analysis

DGGE Analysis Bacterial Community Structure of Advanced Sludge Reduction Process

2012 ◽  
Vol 178-181 ◽  
pp. 549-552
Author(s):  
De Wei Mu
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Nitrogen Removal ◽  
Bacterial Community Structure ◽  
Reduction Process ◽  
Microbial Flora ◽  
Sludge Reduction ◽  
Technology Research ◽  
Co Activation ◽  
Consistent Function

Bacterial community structure of advanced HA-A/A-MCO sludge reduction process is analyzed by DGGE fingerprinting technology. Research results indicates that microbial flora showed the distinction of highly diversity in anaerobic tank, anoxic tank and MCO tank of the HA-A/A-MCO process, and each tank has its own diversified and stable dominant microorganisms, as so the co-activation of such preponderant bacterial community promotes the system to exert favourable and consistent function of phosphorous and nitrogen removal and sludge reduction.

scholarly journals Dynamics of Bacterial Community Structure in the Rhizosphere and Root Nodule of Soybean: Impacts of Growth Stages and Varieties

2021 ◽  
Author(s):  
Soo-In Sohn ◽  
Jae-Hyung Ahn ◽  
Subramani Pandian ◽  
Young-Ju Oh ◽  
Eun-Kyoung Shin ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Bacterial Community Structure ◽  
High Throughput Sequencing ◽  
Root Nodules ◽  
Growth Stages ◽  
Sequencing Analysis ◽  
Biolog Ecoplate ◽  
Metabolic Capability ◽  
Increasing Trend

Bacterial communities in rhizosphere and root nodules have significant contributions to the growth and productivity of the soybean (Glycine max L.). In this report, we analyzed the physiological properties and dynamics of bacterial community structure in rhizosphere and root nodules at different growth stages using BioLog EcoPlate and high-throughput sequencing technology, respectively. The BioLog assay found that the metabolic capability of rhizosphere is in increasing trend in the growth of soybeans as compared to the bulk soil. As a result of the Illumina sequencing analysis, the microbial community structure of rhizosphere and root nodules was found to be influenced by the variety and growth stage of the soybean. At the phylum level, Actinobacteria were the most abundant in rhizosphere at all growth stages, followed by Alphaproteobacteria and Acidobacteria and the phylum Bacteroidetes showed the greatest change. But, in the root nodules Alphaproteobacteria were dominant. The results of the OTU analysis exhibited the dominance of Bradyrhizobium during the entire stage of growth, but the ratio of non-rhizobial bacteria showed an increasing trend as the soybean growth progressed. These findings revealed that bacterial community in the rhizosphere and root nodules changed according to both the variety and growth stages of soybean in the field.

scholarly journals Purification Effects on β-HCH Removal and Bacterial Community Differences of Vertical-Flow Constructed Wetlands with Different Vegetation Plantations

2021 ◽  
Vol 13 (23) ◽  
pp. 13244
Author(s):  
Qing Chen ◽  
Honghu Zeng ◽  
Yanpeng Liang ◽  
Litang Qin ◽  
Guangsheng Peng ◽  
...  
Keyword(s):  
Community Structure ◽  
Bacterial Community ◽  
Plant Species ◽  
Constructed Wetlands ◽  
Bacterial Community Structure ◽  
High Throughput Sequencing ◽  
Community Response ◽  
Control Treatment ◽  
Acorus Calamus ◽  
Sequencing Analysis

This study aimed to investigate the removal of β-hexachlorocyclohexane (β-HCH) at realistic concentration levels (10 µg/L) in different plant species in constructed wetlands (Acorus calamus, Canna indica, Thalia dealbata, and Pontederia cordata) and the structure of the rhizosphere microbial community response of each group during summer and winter. Results showed that all groups of constructed wetlands had very good decontamination efficiency against β-HCH in water (90.86–98.17%). The species that most efficiently purified β-HCH in water was A. calamus in summer (98.17%) and C. indica in winter (96.64%). Substrate sorption was found to be the major pathway for β-HCH removal from water in the constructed wetlands. The ability of the wetland plants to absorb and purify β-HCH was limited, and C. indica had the strongest absorptive capacity among the four plant species. The mean β-HCH removal from the matrix of the planted plants increased by 5.8% compared with that of the control treatment (unplanted plants). The average β-HCH content in the plant rhizosphere substrate was 4.15 µg/kg lower than that in the non-rhizosphere substrate. High-throughput sequencing analysis revealed significant differences (P < 0.05) in the Chao1 and ACE indices of microbes in the substrate of four wetlands during summer and winter. At the genus level, the constructed wetlands with vegetation plantations showed higher microbial abundance than the constructed wetlands without vegetation plantations. In winter, the bacterial community structure of each constructed wetland was quite different, but no dominant flora in the bacterial community structure obviously changed. In summer, the bacterial community structure at the same stage was relatively small. The abundance of Actinobacteria and Sphingomonas remarkably increased over time in summer.

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