Purification Effects on β-HCH Removal and Bacterial Community Differences of Vertical-Flow Constructed Wetlands with Different Vegetation Plantations

This study aimed to investigate the removal of β-hexachlorocyclohexane (β-HCH) at realistic concentration levels (10 µg/L) in different plant species in constructed wetlands (Acorus calamus, Canna indica, Thalia dealbata, and Pontederia cordata) and the structure of the rhizosphere microbial community response of each group during summer and winter. Results showed that all groups of constructed wetlands had very good decontamination efficiency against β-HCH in water (90.86–98.17%). The species that most efficiently purified β-HCH in water was A. calamus in summer (98.17%) and C. indica in winter (96.64%). Substrate sorption was found to be the major pathway for β-HCH removal from water in the constructed wetlands. The ability of the wetland plants to absorb and purify β-HCH was limited, and C. indica had the strongest absorptive capacity among the four plant species. The mean β-HCH removal from the matrix of the planted plants increased by 5.8% compared with that of the control treatment (unplanted plants). The average β-HCH content in the plant rhizosphere substrate was 4.15 µg/kg lower than that in the non-rhizosphere substrate. High-throughput sequencing analysis revealed significant differences (P < 0.05) in the Chao1 and ACE indices of microbes in the substrate of four wetlands during summer and winter. At the genus level, the constructed wetlands with vegetation plantations showed higher microbial abundance than the constructed wetlands without vegetation plantations. In winter, the bacterial community structure of each constructed wetland was quite different, but no dominant flora in the bacterial community structure obviously changed. In summer, the bacterial community structure at the same stage was relatively small. The abundance of Actinobacteria and Sphingomonas remarkably increased over time in summer.

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Effects of Phytoremediation Treatment on Bacterial Community Structure and Diversity in Different Petroleum-Contaminated Soils

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph15102168 ◽

2018 ◽

Vol 15 (10) ◽

pp. 2168 ◽

Cited By ~ 9

Author(s):

Yuanyuan Shen ◽

Yu Ji ◽

Chunrong Li ◽

Pingping Luo ◽

Wenke Wang ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Contaminated Soils ◽

Bacterial Community Structure ◽

High Throughput Sequencing ◽

Cost Effective ◽

Northwest China ◽

Community Response ◽

Available Nitrogen ◽

Increased Risk

Increased exploitation and use of petroleum resources is leading to increased risk of petroleum contamination of soil and groundwater. Although phytoremediation is a widely-used and cost-effective method for rehabilitating soils polluted by petroleum, bacterial community structure and diversity in soils undergoing phytoremediation is poorly understood. We investigate bacterial community response to phytoremediation in two distinct petroleum-contaminated soils (add prepared petroleum-contaminated soils) from northwest China, Weihe Terrace soil and silty loam from loess tableland. High-throughput sequencing technology was used to compare the bacterial communities in 24 different samples, yielding 18,670 operational taxonomic units (OTUs). The dominant bacterial groups, Proteobacteria (31.92%), Actinobacteria (16.67%), Acidobacteria (13.29%) and Bacteroidetes (6.58%), increased with increasing petroleum concentration from 3000 mg/kg–10,000 mg/kg, while Crenarchaeota (13.58%) and Chloroflexi (4.7%) decreased. At the order level, RB41, Actinomycetales, Cytophagales, envOPS12, Rhodospirillales, MND1 and Xanthomonadales, except Nitrososphaerales, were dominant in Weihe Terrace soil. Bacterial community structure and diversity in the two soils were significantly different at similar petroleum concentrations. In addition, the dominant genera were affected by available nitrogen, which is strongly associated with the plants used for remediation. Overall, the bacterial community structure and diversity were markedly different in the two soils, depending on the species of plants used and the petroleum concentration.

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Dynamics of Bacterial Community Structure in the Rhizosphere and Root Nodule of Soybean: Impacts of Growth Stages and Varieties

10.20944/preprints202105.0460.v1 ◽

2021 ◽

Author(s):

Soo-In Sohn ◽

Jae-Hyung Ahn ◽

Subramani Pandian ◽

Young-Ju Oh ◽

Eun-Kyoung Shin ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure ◽

High Throughput Sequencing ◽

Root Nodules ◽

Growth Stages ◽

Sequencing Analysis ◽

Biolog Ecoplate ◽

Metabolic Capability ◽

Increasing Trend

Bacterial communities in rhizosphere and root nodules have significant contributions to the growth and productivity of the soybean (Glycine max L.). In this report, we analyzed the physiological properties and dynamics of bacterial community structure in rhizosphere and root nodules at different growth stages using BioLog EcoPlate and high-throughput sequencing technology, respectively. The BioLog assay found that the metabolic capability of rhizosphere is in increasing trend in the growth of soybeans as compared to the bulk soil. As a result of the Illumina sequencing analysis, the microbial community structure of rhizosphere and root nodules was found to be influenced by the variety and growth stage of the soybean. At the phylum level, Actinobacteria were the most abundant in rhizosphere at all growth stages, followed by Alphaproteobacteria and Acidobacteria and the phylum Bacteroidetes showed the greatest change. But, in the root nodules Alphaproteobacteria were dominant. The results of the OTU analysis exhibited the dominance of Bradyrhizobium during the entire stage of growth, but the ratio of non-rhizobial bacteria showed an increasing trend as the soybean growth progressed. These findings revealed that bacterial community in the rhizosphere and root nodules changed according to both the variety and growth stages of soybean in the field.

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Dynamics of Bacterial Community Structure in the Rhizosphere and Root Nodule of Soybean: Impacts of Growth Stages and Varieties

International Journal of Molecular Sciences ◽

10.3390/ijms22115577 ◽

2021 ◽

Vol 22 (11) ◽

pp. 5577

Author(s):

Soo-In Sohn ◽

Jae-Hyung Ahn ◽

Subramani Pandian ◽

Young-Ju Oh ◽

Eun-Kyoung Shin ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure ◽

High Throughput Sequencing ◽

Root Nodules ◽

Growth Stages ◽

Sequencing Analysis ◽

Biolog Ecoplate ◽

Metabolic Capability ◽

Increasing Trend

Bacterial communities in rhizosphere and root nodules have significant contributions to the growth and productivity of the soybean (Glycine max (L.) Merr.). In this report, we analyzed the physiological properties and dynamics of bacterial community structure in rhizosphere and root nodules at different growth stages using BioLog EcoPlate and high-throughput sequencing technology, respectively. The BioLog assay found that the metabolic capability of rhizosphere is in increasing trend in the growth of soybeans as compared to the bulk soil. As a result of the Illumina sequencing analysis, the microbial community structure of rhizosphere and root nodules was found to be influenced by the variety and growth stage of the soybean. At the phylum level, Actinobacteria were the most abundant in rhizosphere at all growth stages, followed by Alphaproteobacteria and Acidobacteria, and the phylum Bacteroidetes showed the greatest change. But, in the root nodules Alphaproteobacteria were dominant. The results of the OTU analysis exhibited the dominance of Bradyrhizobium during the entire stage of growth, but the ratio of non-rhizobial bacteria showed an increasing trend as the soybean growth progressed. These findings revealed that bacterial community in the rhizosphere and root nodules changed according to both the variety and growth stages of soybean in the field.

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High throughput sequencing analysis of the joint effects of BDE209-Pb on soil bacterial community structure

Journal of Hazardous Materials ◽

10.1016/j.jhazmat.2015.08.037 ◽

2016 ◽

Vol 301 ◽

pp. 1-7 ◽

Cited By ~ 48

Author(s):

Wei Zhang ◽

Lei Chen ◽

Rong Zhang ◽

Kuangfei Lin

Keyword(s):

Community Structure ◽

Bacterial Community ◽

High Throughput ◽

Bacterial Community Structure ◽

High Throughput Sequencing ◽

Soil Bacterial Community ◽

Sequencing Analysis ◽

Joint Effects ◽

Soil Bacterial Community Structure ◽

Soil Bacterial

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Bacterial community structure and functional profiling of high Arctic fjord sediments

10.21203/rs.3.rs-187209/v1 ◽

2021 ◽

Author(s):

S Vishnupriya ◽

JABIR T ◽

K.P Krishnan ◽

Abdulla Mohamed Hatha

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure ◽

High Throughput Sequencing ◽

High Arctic ◽

The Arctic ◽

Sequencing Analysis ◽

Subsurface Sediments ◽

Functional Profiling ◽

Arctic Fjord

Abstract Kongsfjorden, an Arctic fjord is significantly affected by the glacier melt and Atlantification, both the processes driven by accelerated warming in the Arctic. This has lead to changes in primary production, carbon pool and microbial communities, especially that in the sediment. In this study, we have examined the bacterial community structure of surface (0–2 cm) and subsurface (3–9 cm) sediments of Kongsfjorden using the high throughput sequencing analysis. Results revealed that bacterial community structure of Kongsfjorden sediments were dominated by phylum Proteobacteria followed by Bacteroidetes and Epsilonbacteraeota. While α- and γ- Proteobacterial class were dominant in surface sediments; δ- Proteobacteria were found to be predominant in subsurface sediments. The bacterial community structure in the surface and subsurface sediments showed significant variations (p ≤ 0.05). Total organic carbon could be one of the major parameters controlling the bacterial diversity in the surface and subsurface sediments. Functional prediction analysis indicated that the bacterial community could be involved in the degradation of complex organic compounds such as glycans, glycosaminoglycans, polycyclic aromatic hydrocarbons and also in the biosynthesis of secondary metabolites.

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Size Matters: Assessing Optimum Soil Sample Size for Fungal and Bacterial Community Structure Analyses Using High Throughput Sequencing of rRNA Gene Amplicons

Frontiers in Microbiology ◽

10.3389/fmicb.2016.00824 ◽

2016 ◽

Vol 7 ◽

Cited By ~ 25

Author(s):

C. Ryan Penton ◽

Vadakattu V. S. R. Gupta ◽

Julian Yu ◽

James M. Tiedje

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Sample Size ◽

Soil Sample ◽

High Throughput ◽

Bacterial Community Structure ◽

High Throughput Sequencing ◽

Rrna Gene

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Comparison of Auxin and Cytokinins Concentrations, and the Structure of Bacterial Community between Host Twigs and Lithosaphonecrus arcoverticus Galls

Insects ◽

10.3390/insects12110982 ◽

2021 ◽

Vol 12 (11) ◽

pp. 982

Author(s):

Xue-Mei Yang ◽

Yu Hui ◽

Lv-Quan Zhao ◽

Dao-Hong Zhu ◽

Yang Zeng ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Bacterial Community Structure ◽

High Throughput Sequencing ◽

Zeatin Riboside ◽

Insect Galls ◽

Abnormal Growth ◽

Liquid Chromatography Tandem Mass ◽

High Concentrations ◽

Galling Insects

Insect galls are the abnormal growth of plant tissues induced by a wide variety of galling insects and characterized by high concentrations of auxins and cytokinins. It remains unclear whether the auxins and cytokinins affect the bacterial community structure of insect galls. We determined the concentrations of indoleacetic acid (IAA) as an example of auxin, trans-zeatin riboside (tZR) and isopentenyladenine (iP) as cytokinins in Lithosaphonecrus arcoverticus (Hymenoptera: Cynipidae) galls and the galled twigs of Lithocarpus glaber (Fagaceae) using liquid chromatography–tandem mass spectrometry. Moreover, for the first time, we compared the bacterial community structure of L. arcoverticus galls and galled twigs by high-throughput sequencing, and calculated the Spearman correlation and associated degree of significance between the IAA, tZR and iP concentrations and the bacterial community structure. Our results indicated the concentrations of IAA, tZR and iP were higher in L. arcoverticus galls than in galled twigs, and positively correlated with the bacterial community structure of L. arcoverticus galls. We suggest the high concentrations of IAA, tZR and iP may affect the bacterial community structure of L. arcoverticus galls.

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Effects of Continuous Cropping on Bacterial Community Structure in Rhizospheric Soil of Sweet Potato

10.21203/rs.3.rs-61006/v1 ◽

2020 ◽

Author(s):

Zhiyuan Gao ◽

Yaya Hu ◽

Meikun Han ◽

Junjie Xu ◽

Xue Wang ◽

...

Keyword(s):

Community Structure ◽

Bacterial Community ◽

Sweet Potato ◽

Soil Ph ◽

Bacterial Community Structure ◽

High Throughput Sequencing ◽

Potato Industry ◽

Continuous Cropping ◽

Rhizospheric Soil ◽

Potato Varieties

Abstract Background: Continuous cropping obstacles from sweet potato are widespread, which seriously reduce the yield and quality, restrict the sustainable development of sweet potato industry. Bacteria are the most abundant in rhizospheric soil and have a certain relationship with continuous cropping obstacles. However, there are few reports on how continuous cropping affected the bacterial community structure in the rhizospheric soil of sweet potato. In this study, high-throughput sequencing technique was used to explore the changes of rhizospheric soil bacterial community structure of different sweet potato varieties, and the correlation between soil characteristics and this bacterial community after continuous cropping, so as to provide a theoretical basis for the prevention and control of sweet potato continuous cropping obstacles.Results: After two years of continuous cropping, the results showed that (1) the dominant bacteria phlya in rhizospheric soils from both Xushu18 and Yizi138 were Proteobacteria, Acidobacteria, and Actinobacteria. The most dominant genus was Subgroup 6_norank. Significant changes in the relative abundance of rhizospheric soil bacteria were observed for two sweet potato varieties. (2) Bacterial richness and diversity indexes of rhizospheric soil from Xushu18 were higher than those from Yizi138 after continuous cropping. Moreover, the beneficial Lysobacter and Bacillus were more prevalent in Xushu18, but Yizi138 contained more harmful Gemmatimonadetes. (3) Soil pH decreased after continuous cropping, and redundancy analysis showed that soil pH was significantly correlated with bacterial community. Spearman’s rank correlations coefficients analysis demonstrated that pH was positively correlated with Planctomycetes and Acidobacteria, and negatively correlated with Actinobacteria and Firmicutes.Conclusions: After continuous cropping of sweet potato, the bacterial community structure and physicochemical properties in the rhizospheric soil were unbalanced, and the changes of different sweet potato varieties were different. The contents of Lysobacter and Bacillus were higher in the sweet potato variety resistant to continuous cropping. It provides a basis for the development of special microbial fertilizer for sweet potatoes to alleviate continuous cropping obstacle.

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