Optimizing the Fermentation Process for Recombinant Escherichia coli on Base of Metabolic Flux Analysis of Amino Acids

Amino acids, the basic unit of protein molecules, are closely related to some important biological activities and they affect cell growth and metabolism directly or indirectly. Previous studies showed that adding amino acids can improve the productivity of human-like collagen (HLC). Thus, we analyzed the amino acids metabolism during the fermentation process. The results of metabolic flux analysis of amino acids implied that the cell growth and production of HLC were active as long as the free amino acids in the medium were adequate. This suggested control the concentration of amino acids could improve cell growth and human-like collagen production.