293SF Metabolic Flux Analysis during Cell Growth and Infection with an Adenoviral Vector

2000 ◽  
Vol 16 (5) ◽  
pp. 872-884 ◽  
Author(s):  
I. Nadeau ◽  
D. Jacob ◽  
M. Perrier ◽  
A. Kamen
Keyword(s):  
Cell Growth ◽  
Metabolic Flux Analysis ◽  
Adenoviral Vector ◽  
Metabolic Flux ◽  
Flux Analysis

Optimizing the Fermentation Process for Recombinant Escherichia coli on Base of Metabolic Flux Analysis of Amino Acids

2012 ◽  
Vol 550-553 ◽  
pp. 1055-1059
Author(s):  
Tao Zhang ◽  
Yan E Luo ◽  
Dai Di Fan ◽  
Lei Guo ◽  
Ting Zhen Mu
Keyword(s):  
Amino Acids ◽  
Cell Growth ◽  
Metabolic Flux Analysis ◽  
Fermentation Process ◽  
Metabolic Flux ◽  
Biological Activities ◽  
Basic Unit ◽  
Flux Analysis ◽  
Protein Molecules ◽  
Affect Cell Growth

Amino acids, the basic unit of protein molecules, are closely related to some important biological activities and they affect cell growth and metabolism directly or indirectly. Previous studies showed that adding amino acids can improve the productivity of human-like collagen (HLC). Thus, we analyzed the amino acids metabolism during the fermentation process. The results of metabolic flux analysis of amino acids implied that the cell growth and production of HLC were active as long as the free amino acids in the medium were adequate. This suggested control the concentration of amino acids could improve cell growth and human-like collagen production.

Intracellular metabolic flux analysis of CHO cells supplemented with wheat hydrolysates for improved mAb production and cell-growth

2014 ◽  
Vol 90 (2) ◽  
pp. 291-302 ◽  
Author(s):  
Hae Woo Lee ◽  
Andrew Christie ◽  
Jason A. Starkey ◽  
Erik K. Read ◽  
Seongkyu Yoon
Keyword(s):  
Cell Growth ◽  
Metabolic Flux Analysis ◽  
Cho Cells ◽  
Metabolic Flux ◽  
Flux Analysis ◽  
Mab Production
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