Selective Determination of Metformin in Urine Sample Based on Molecularly Imprinted Polymer-Chemiluminescence Sensor

2012 ◽  
Vol 550-553 ◽  
pp. 798-801
Author(s):  
Yan Xiong ◽  
Ying Huang ◽  
Zhong Bin Ye ◽  
Chen Chen ◽  
Zhu Jun Zhang

A flow-injection chemiluminescence (FI-CL) sensor for metformin determination based on recognition materials of molecularly imprinted polymers (MIP) is reported. The sensor was fabricated by packing the MIP particles into a V-shape flow cell. Metformin could be selectively on-line adsorbed by the MIPs and then sensed by its great enhancing effect on the weak CL reaction between N-bromosuccinimide (NBS) and fluorescein. The CL intensity was linear to metformin concentration in the range from 2×10−8 to 8×10−6 g•mL−1 and the limit of detection (L.O.D.) was 6×10−9 g•mL−1 (3σ). The sensor showed high selectivity, improved sensitivity and excellent stability and reversibility compared with the CL method without using MIPs. The lifetime of the sensor was investigated and MIPs could be reused for more than 200 times. The sensor has been directly applied to determine metformin in human urine.

2012 ◽  
Vol 554-556 ◽  
pp. 1841-1844 ◽  
Author(s):  
Ying Huang ◽  
Yan Xiong ◽  
Zhong Bin Ye ◽  
Zhu Jun Zhang

Using methacrylic acid (MAA) as monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker and phentolamine as template molecule, we synthesized phentolamine–molecularly imprinted polymers (MIP). Using phentolamine–MIP as molecule recognizates, based on the oxidation reaction between KMnO4 and phenolamine in acidic, we set up a flow-injection chemiluminescence (FI-CL) sensor for phentolamine determination. The sensor was fabricated by packing the MIP particles into a V-shape flow cell. Phentolamine could be selectively on-line adsorbed by the MIPs and then sensed by the oxidation reaction between KMnO4 and phenolamine in acidic.The CL intensity was linear to phentolamine concentration in the range from 2.0 ×10−8 to 6.0 ×10−6 g mL−1 and the limit of detection (L.O.D.) was 6.0 ×10−9 g mL−1 (3σ) with the relative standard deviation (R.S.D.) less than 5% (n=11). The sensor showed high selectivity, improved sensitivity and excellent stability and reversibility compared with the CL method without using MIPs. The lifetime of the sensor was investigated and MIPs could be reused for more than 200 times. Using the method to determine phentolamine in human urine, the recoveries of phentolamine is 100.0% to 104.0%, the result is pretty good.


RSC Advances ◽  
2016 ◽  
Vol 6 (60) ◽  
pp. 54702-54708 ◽  
Author(s):  
Mostafa Khajeh ◽  
Marzieh Sharifirad ◽  
Mousa Bohlooli ◽  
Mansour Ghaffari-Moghaddam

In this study, an efficient and sensitive magnetic molecularly imprinted polymer–silver nanoparticle (MMIPS) system was successfully synthesized.


Author(s):  
Jyoti . ◽  
Renata Rybakiewicz ◽  
Teresa Zolek ◽  
Dorota Maciejewska ◽  
Edyta Gilant ◽  
...  

An electrochemical chemosensor for cilostazol (CIL) determination was devised, engineered, and tested. For that, a unique conducting film of the functionalized thiophene-appended carbazole-based polymer, molecularly imprinted with cilostazol (MIP-CIL), was...


RSC Advances ◽  
2017 ◽  
Vol 7 (62) ◽  
pp. 38884-38894 ◽  
Author(s):  
Tianjiao Xie ◽  
Min Zhang ◽  
Pan Chen ◽  
Haitian Zhao ◽  
Xin Yang ◽  
...  

In this study, we report a facile method for the preparation of molecularly imprinted polymer based graphene for the electrochemical detection of thiamethoxam residue.


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