Molecularly Imprinted Polymer-Chemiluminescence Sensor Detect Phentolamine in Urine Sample

2012 ◽  
Vol 554-556 ◽  
pp. 1841-1844 ◽  
Author(s):  
Ying Huang ◽  
Yan Xiong ◽  
Zhong Bin Ye ◽  
Zhu Jun Zhang
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Oxidation Reaction ◽  
Limit Of Detection ◽  
Template Molecule ◽  
Molecularly Imprinted ◽  
Relative Standard ◽  
On Line ◽  
Set Up ◽  
Flow Injection Chemiluminescence ◽  
Excellent Stability

Using methacrylic acid (MAA) as monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker and phentolamine as template molecule, we synthesized phentolamine–molecularly imprinted polymers (MIP). Using phentolamine–MIP as molecule recognizates, based on the oxidation reaction between KMnO4 and phenolamine in acidic, we set up a flow-injection chemiluminescence (FI-CL) sensor for phentolamine determination. The sensor was fabricated by packing the MIP particles into a V-shape flow cell. Phentolamine could be selectively on-line adsorbed by the MIPs and then sensed by the oxidation reaction between KMnO4 and phenolamine in acidic.The CL intensity was linear to phentolamine concentration in the range from 2.0 ×10−8 to 6.0 ×10−6 g mL−1 and the limit of detection (L.O.D.) was 6.0 ×10−9 g mL−1 (3σ) with the relative standard deviation (R.S.D.) less than 5% (n=11). The sensor showed high selectivity, improved sensitivity and excellent stability and reversibility compared with the CL method without using MIPs. The lifetime of the sensor was investigated and MIPs could be reused for more than 200 times. Using the method to determine phentolamine in human urine, the recoveries of phentolamine is 100.0% to 104.0%, the result is pretty good.

Selective Determination of Metformin in Urine Sample Based on Molecularly Imprinted Polymer-Chemiluminescence Sensor

2012 ◽  
Vol 550-553 ◽  
pp. 798-801
Author(s):  
Yan Xiong ◽  
Ying Huang ◽  
Zhong Bin Ye ◽  
Chen Chen ◽  
Zhu Jun Zhang
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Limit Of Detection ◽  
Imprinted Polymer ◽  
Molecularly Imprinted ◽  
Selective Determination ◽  
Metformin Concentration ◽  
On Line ◽  
Flow Injection Chemiluminescence ◽  
Excellent Stability

A flow-injection chemiluminescence (FI-CL) sensor for metformin determination based on recognition materials of molecularly imprinted polymers (MIP) is reported. The sensor was fabricated by packing the MIP particles into a V-shape flow cell. Metformin could be selectively on-line adsorbed by the MIPs and then sensed by its great enhancing effect on the weak CL reaction between N-bromosuccinimide (NBS) and fluorescein. The CL intensity was linear to metformin concentration in the range from 2×10−8 to 8×10−6 g•mL−1 and the limit of detection (L.O.D.) was 6×10−9 g•mL−1 (3σ). The sensor showed high selectivity, improved sensitivity and excellent stability and reversibility compared with the CL method without using MIPs. The lifetime of the sensor was investigated and MIPs could be reused for more than 200 times. The sensor has been directly applied to determine metformin in human urine.

scholarly journals Preparation of Monodisperse Enrofloxacin Molecularly Imprinted Polymer Microspheres and Their Recognition Characteristics

2019 ◽  
Vol 2019 ◽  
pp. 1-10
Author(s):  
Xiaoxiao Wang ◽  
Yanqiang Zhou ◽  
Yuling Niu ◽  
Shanwen Zhao ◽  
Bolin Gong
Keyword(s):  
Molecularly Imprinted Polymers ◽  
High Performance ◽  
Limit Of Detection ◽  
Solid Phase ◽  
Food Samples ◽  
Diglycidyl Ether ◽  
Template Molecule ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Relative Standard

This study presents a new strategy for the detection of enrofloxacin (ENR) in food samples by the use of monodisperse ENR molecularly imprinted polymers (MIPs). Using enrofloxacin as template molecule, methacrylic acid as functional monomer, and ethylene diglycidyl ether as cross-linker, surface molecularly imprinted polymers (MIPs) were prepared on the surface of polymeric glycidyl methacrylate-ethylene glycol dimethacrylate (PGMA-EDMA) microspheres. The surface morphology and imprinting behavior of PGMA-EDMA@MIPs were investigated and optimized. Synthesized PGMA-EDMA@MIPs showed good physical and chemical stability and specific recognition toward fluoroquinolones. The introduction of PGMA-EDMA microspheres greatly increased the adsorption area of PGMA-EDMA@MIPs and increased the adsorption capacity of target molecules. The core shell structure increased the adsorption rate, and adsorption equilibrium was achieved within 6 min, much higher than that of MIPs synthesized by traditional methods. Enrofloxacin in milk samples was detected by molecular imprinting solid phase extraction (MISPE) combined with high performance liquid chromatography (HPLC). Implementing this method resulted in a recovery rate of 94.6~109.6% with a relative standard deviation (RSD) of less than 3.2%. The limit of detection (LOD) of this method was identified as three times the signal-to-noise ratio (10 μg/L). In summary, this work proposed a sensitive, rapid, and convenient method for the determination of trace ENR in food samples.

scholarly journals Synthesis and characterization of genistein magnetic molecularly imprinted polymers and their application in soy sauce products

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Ziqi Xie ◽  
Yunjing Luo ◽  
Zhen Na ◽  
Wei Zhang ◽  
Yufei Zong
Keyword(s):  
Electron Microscopy ◽  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Soy Sauce ◽  
Template Molecule ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Magnetic Molecularly Imprinted Polymers ◽  
Relative Standard ◽  
Surface Molecular Imprinting Technique

AbstractIn this study, a novel method based on genistein magnetic molecularly imprinted polymers (Gen-MMIPs) was developed utilizing a surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. The synthesis of Gen-MMIPs was characterized by using scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which indicated that the diameter of the Gen-MMIPs was approximately 500 nm. Via analysis with a vibrating sample magnetometer (VSM), the saturation magnetization of Gen-MMIPs was determined to be 24.79 emu g−1. Fourier transform infrared (FT-IR) spectroscopy showed that polymer groups were on the surface of the magnetic carrier. Adsorption experiments suggested that the genistein adsorption capability of Gen-MMIPs was 5.81 mg g−1, and adsorption equilibrium was achieved within 20 min. Gen-MMIPs as dispersive solid-phase extraction (dSPE) adsorbents combined with HPLC were used to selectively separate genistein in soy sauce samples, and the recoveries ranged from 85.7 to 88.5% with relative standard deviations (RSDs) less than 5%, which proved that this method can be used for the detection of genistein residues in real samples.

scholarly journals Synthesis and Characterization of Genistein Magnetic Molecularly Imprinted Polymers and Its Application in Soy Sauce Products

2021 ◽  
Author(s):  
Ziqi Xie ◽  
Yunjing Luo ◽  
Zhen Na ◽  
Wei Zhang ◽  
Yunfei Zong
Keyword(s):  
Electron Microscopy ◽  
Molecularly Imprinted Polymers ◽  
Solid Phase ◽  
Soy Sauce ◽  
Template Molecule ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Magnetic Molecularly Imprinted Polymers ◽  
Relative Standard ◽  
Surface Molecular Imprinting Technique

Abstract In this study, a novel method based on genistein magnetic molecularly imprinted polymers (Gen-MMIPs) was developed by surface molecular imprinting technique, in which genistein was used as the template molecule and Fe3O4 was used as the carrier. The synthesis of Gen-MMIPs were characterized by using of scanning electron microscopy (SEM) and transmission electron microscopy (TEM), which indicated the diameters of Gen-MMIPs were about 500 nm. Through the technique of vibrating sample magnetometer (VSM), the saturation magnetization of Gen-MMIPs were detected as 24.79 emu/g. Fourier transform infrared (FR-IR) spectroscopy showed that polymer groups were on the surface of the magnetic carrier. Adsorption experiment suggested the adsorption capability of Gen-MMIPs to genistein were 1.55 mg/g, and 2 the adsorption equilibrium was achieved within 20 min. Gen-MMIPs as dispersive solid-phase extraction adsorbent combined with HPLC was used to selectively separate genistein in soy sauce samples, the recoveries were ranged from 85.7% to 88.5% with the relative standard deviations (RSD) less than 5%, which proved this method can be used for the detection of genistein residues in real samples.

scholarly journals Preparation of Restricted Access Media-Molecularly Imprinted Polymers for the Detection of Chloramphenicol in Bovine Serum

2019 ◽  
Vol 2019 ◽  
pp. 1-12
Author(s):  
Shanwen Zhao ◽  
Chanling Wei ◽  
Zhian Sun ◽  
Huachun Liu ◽  
Yanqiang Zhou ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Bovine Serum ◽  
Crosslinking Agent ◽  
Template Molecule ◽  
Functional Monomer ◽  
Molecularly Imprinted ◽  
Restricted Access ◽  
Imprinted Polymers ◽  
Relative Standard ◽  
Restricted Access Media

Chloramphenicol- (CAP-) restricted access media-molecularly imprinted polymers (CAP-RAM-MIPs) were prepared by precipitation polymerization using CAP as a template molecule, 2-diethylaminoethyl methacrylate (DEAEM) as a functional monomer, ethylene glycol dimethyl acrylate (EDMA) as a crosslinking agent, glycidyl methacrylate (GMA) as an outer hydrophilic functional monomer, and acetonitrile as a pore former and solvent. The CAP-RAM-MIPs were successfully characterized by Fourier-transform infrared spectroscopy, scanning electron microscopy, and thermogravimetric analysis. The adsorption performance was investigated in detail using static, dynamic, and selective adsorption experiments. Adsorption equilibrium could be reached within 11 min. The CAP-RAM-MIPs had a high adsorption rate and good specific adsorption properties. Scatchard fitting curves indicated there were two binding sites for CAP-RAM-MIPs. Adsorption was Freundlich multilayer adsorption and consistent with the quasi-second kinetic model. Using CAP-RAM-MIPs for selective separation and enrichment CAP in bovine serum in combination with high-performance liquid chromatography (HPLC), CAP recovery ranged from 94.1 to 97.9% with relative standard deviations of 0.7–1.5%. This material has broad application prospects in enrichment and separation.

scholarly journals A Novel Method for Extraction of Galegine by Molecularly Imprinted Polymer (MIP) Technique Reinforced with Graphene Oxide and Its Evaluation Using Polarography

2020 ◽  
Vol 2020 ◽  
pp. 1-9
Author(s):  
M. Azimi ◽  
M. Ahmadi Golsefidi ◽  
A. Varasteh Moradi ◽  
M. Ebadii ◽  
R. Zafar Mehrabian
Keyword(s):  
Molecularly Imprinted Polymer ◽  
Limit Of Detection ◽  
Diabetes Type 2 ◽  
Differential Pulse ◽  
Template Molecule ◽  
Imprinted Polymer ◽  
Limit Of Quantification ◽  
Molecularly Imprinted ◽  
Galega Officinalis ◽  
Relative Standard

Galega officinalis products have been used for the control of diabetes (type 2) across the world. Experimental and clinical evaluations of galegine substance produced by a medicinal plant (Galega officinalis) provided the pharmacological and chemical basis for metformin discovery which was confirmed for diabetes therapy. In this paper, the molecularly imprinted polymer (MIP) was synthesized for galegine, using galegine as a template molecule, methacrylic acid (MAA) as a functional monomer, ethylene glycol dimethacrylate (EGDMA) as a cross-linker, azobisisobutyronitrile (AIBN) as a reaction initiator, and acetonitrile as a solvent. The assisted functional groups, morphology, topographic image of surface, and crystalline structure of synthesized MIP were characterized by FT-IR spectroscopy, field emission scanning electron microscopy (FE-SEM), atomic force microscopy (AFM) images, and XRD diffraction pattern techniques, respectively. Also, the performance of the mentioned electrode was quantified and qualified by the differential pulse voltammetry technique (DPV). The galegine amount was determined with the polarographic technique. In this research, the galegine extraction conditions were optimized and graphene nanoparticles were used to increase the adsorption. In addition, different parameters affecting extraction were investigated such as MIP adsorbent amount, pH of solution, effect of the surfactant, and ionic compound to achieve high recovery percent. The recovery percent, limit of detection (LOD), limit of quantification (LOQ), and relative standard deviation (RSD %) were 4.101 μg·mL−1, 12.427 μg·mL−1, and 1.199% (n = 3), respectively. The results show that the prepared MIP can be used as an effective and inexpensive adsorbent for preconcentration and galegine extraction from a natural sample. It is noteworthy that this developed method was used successfully to determine galegine extracted from Galega officinalis L.

scholarly journals Oriented, molecularly imprinted cavities with dual binding sites for highly sensitive and selective recognition of cortisol

2017 ◽  
Vol 4 (8) ◽  
pp. 170300 ◽  
Author(s):  
Narito Suda ◽  
Hirobumi Sunayama ◽  
Yukiya Kitayama ◽  
Yuri Kamon ◽  
Toshifumi Takeuchi
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Binding Sites ◽  
Limit Of Detection ◽  
Selective Recognition ◽  
Hydroxyl Group ◽  
Template Molecule ◽  
Competitive Binding Assay ◽  
Molecularly Imprinted ◽  
Oxime Formation ◽  
Highly Sensitive

Novel, molecularly imprinted polymers (MIPs) were developed for the highly sensitive and selective recognition of the stress marker cortisol. Oriented, homogeneous cavities with two binding sites for cortisol were fabricated by surface-initiated atom transfer radical polymerization, using a cortisol motif template molecule (TM1) which consists of a polymerizable moiety attached at the 3-carbonyl group of cortisol via an oxime linkage and an adamantane carboxylate moiety coupled with the 21-hydroxyl group. TM1 was orientationally immobilized on a β - cyclodextrin (β-CD)-grafted gold-coated sensor chip by inclusion of the adamantane moiety of TM1, followed by copolymerization of a hydrophilic comonomer, 2-methacryloyloxyethyl phosphorylcholine, with or without a cross-linker, N,N′ -methylenebisacrylamide. Subsequent cleavage of the oxime linkage leaves the imprinted cavities that contain dual binding sites—namely, the aminooxy group and β-CD—capable of oxime formation and hydrophobic interaction, respectively. As an application, MIP-based picomolar level detection of cortisol was demonstrated by a competitive binding assay using a fluorescent competitor. Cross-linking of the MIP imparts rigidity to the binding cavities, and improves the selectivity and sensitivity significantly, reducing the limit of detection to 4.8 pM. In addition, detection of cortisol in saliva samples was demonstrated as a feasibility study.

scholarly journals Towards A New Approach for the Description of Cyclo–2,4-Dihydroxybenzoate, A Substance Which Effectively Mimics Zearalenone in Imprinted Polymers Designed for Analyzing Selected Mycotoxins in Urine

2019 ◽  
Vol 20 (7) ◽  
pp. 1588
Author(s):  
Renata Gadzała-Kopciuch ◽  
Katarzyna Kwaśniewska ◽  
Agnieszka Ludwiczak ◽  
Piotr Skrzyniarz ◽  
Rafał Jakubowski ◽  
...  
Keyword(s):  
Molecularly Imprinted Polymers ◽  
Physicochemical Characteristics ◽  
Crystallographic Analysis ◽  
Template Molecule ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Relative Standard ◽  
First Time

A method of purifying cyclododecyl 2,4-dihydroxybenzoate as a potential replacement template molecule for preparation of molecularly-imprinted polymers for isolation of zearalenone in urine was developed. Full physicochemical characteristics of cyclododecyl 2,4-dihydroxybenzoate for the first time included crystallographic analysis and molecular modelling, which made possible the determination of the similarity between the cyclododecyl 2,4-dihydroxybenzoate and zearalenone molecules. The obtained molecularly-imprinted polymers show very high in vitro selectivity towards zearalenone due to specific interactions (e.g., hydrogen bonding, molecular recognition interaction). The achieved extraction recovery exceeds 94% at the tested concentration levels (20–500 ng·mL−1) with a relative standard deviation below 2%. Immunosorbents were found to have lower recoveries (below 92.5%) and RSD value between 2 and 4% for higher concentrations of the studied substance (400 ng·mL−1).

Microwave-Assisted Preparation of Molecularly Imprinted Monolith Combining Imidazolium Ionic Liquid and POSS for Enhanced Extraction of Baicalin-Like Compounds in Scutellaria Baicalensis by means of In-Capillary SPME Followed by on-line LC and off-line LC-MS/MS

2021 ◽  
Author(s):  
Xiaohan Zhang ◽  
Le Gao ◽  
Liying Niu ◽  
Xiaodong Bi
Keyword(s):  
Ionic Liquid ◽  
Molecularly Imprinted Polymers ◽  
Scutellaria Baicalensis ◽  
Synthetic Methods ◽  
Imidazolium Ionic Liquid ◽  
Molecularly Imprinted ◽  
Imprinted Polymers ◽  
Microwave Assisted ◽  
On Line ◽  
Molecularly Imprinted Monolith

Molecularly imprinted polymers (MIPs) possess target-customized and range-adjustable selectivity, and hence have been attracting increasing efforts to develop new synthetic methods and new forms of applications. By wisely choosing functional...

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