Research on Anti-Coxsackievirus B Activity of Konjac Oligo-Glucomannan Sulfate in Hela Cell Line

2013 ◽  
Vol 662 ◽  
pp. 417-423
Author(s):  
Hao Huang ◽  
Zhong Hua Yang ◽  
Jun Chen
Keyword(s):  
Hela Cell ◽  
Host Cell ◽  
Cell Line ◽  
Rna Replication ◽  
Hela Cell Line ◽  
Rt Pcr ◽  
Coxsackievirus B ◽  
Muench Method

Glucomannan extracted from konjac was sulfated (konjac oligo-glucomannan sulfate, OGMS) to show selective anti- coxsackievirus B activity in vitro using Hela cell line as the host cell of coxsackievirus B. OGMS straight inhibited the virus according to different groups and CVB-RNA Replication in Hela cell by RT-PCR. The studies indicated that OGMS mainly acted as blocking virus invading function and its IC50 was 0.1483 mg/mL determined by Reed-Muench method and CVB-RNA Replication was inhibited as well. All these indicated that OGMS had evident in vitro anti- coxsackievirus B activity.

scholarly journals IN VITRO CYTOTOXICITY POTENTIAL OF ETHANOL EXTRACT OF SYZYGIUM SAMARANGENSE (Wt.)

2019 ◽  
Vol 6 (1) ◽  
pp. 30-32
Author(s):  
Poonkodi K ◽  
Mini R ◽  
Vimaladevi K ◽  
Prabhu V ◽  
Anusuya M ◽  
...  
Keyword(s):  
Fatty Acids ◽  
Hela Cell ◽  
Cell Line ◽  
Ethanol Extract ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Line ◽  
Phytochemical Screening ◽  
Ic50 Value ◽  
Dependent Activity

The present investigation is carried out to study the invitro cytotoxicity of ethanol extract of Syzygium samarangense leaves on HeLa cell line by using MTT assay. Ethanol extract of S. samarangense showed concentration dependent activity on HeLa cell line with IC50 value of 40.5 μg/ml which shows that ethanol extract of S. samarangense posses significant cytoxicity.Moreover the preliminary phytochemical screening showed the presence of fatty acids, alkaloids, flavonoids, terphenoids, saponins, tannins and steroids which are responsible for its cytotoxicity. There are only a few reports are available for cytotoxicity of ethanol extract of S. samarangense.

scholarly journals A COMPARATIVE ANTICANCER STUDY OF BIOSYNTHESIZED NANOPARTICLES, DOXORUBICIN & NANO-DOX CONJUGATE AGAINST CERVICAL CANCER HELA CELL LINE

2020 ◽  
pp. 4-7
Author(s):  
M. R. Kamala Priya ◽  
Priya R. Iyer
Keyword(s):  
Cervical Cancer ◽  
Gold Nanoparticles ◽  
Cell Death ◽  
Cancer Therapy ◽  
Hela Cell ◽  
Cell Line ◽  
In Vitro Cytotoxicity ◽  
Hela Cell Line ◽  
Vero Cell Line

Doxorubicin is the most common chemotherapy drug used in cancer therapy. Its usage is associated with various side-effects. In order to overcome the challenges in Doxorubicin administration, the present study has focussed on synthesizing a drug conjugate with biosynthesized gold nanoparticles and doxorubicin. The gold nanoparticles were biosynthesized using green extracts of medicinal plants with potential anticancer activities. The nanoparticle that possesses anticancer activity was conjugated with the drug for a combinatorial effect of the nanoparticles and the drug. The in vitro cytotoxicity was checked in Vero cell line through MTT assay. The in vitro anti proliferative effects were screened against cervical cancer in HeLa cell line. Fluorescence activated cell sorting analysis was carried out to detect the difference between live and dead cell populations. The preliminary confirmation was carried out in UV-VIS spectrophotometer. The morphological characterization was carried out by SEM and stability by Zeta potential. The IC50 of the nanocompounds demonstrated anti-proliferative activity against cervical cancer similar to the chemotherapeutic drug, Doxorubicin; additionally in a much lesser concentration of the drug. The in vitro cytotoxicity exhibited high viability of cells in Vero cell line with minimum viability of 80% in all the tested concentrations. There was a synergistic effect of the nanoparticles along with the drug; thereby an enhanced therapeutic efficiency was achieved. FACS analysis showed 36% of cell death in Dox treated HeLa cells whereas 96% of cell death in Nano-Dox treated HeLa cells. Nano-Dox conjugate has demonstrated high anticancer effects than drug alone Doxorubicin. Further biosynthesized nanomaterials based drug formulation can be developed as a potential strategy in cancer therapy.

Peptides from Mucuna pruriens L., with protection and antioxidant in vitro effect on HeLa cell line

2019 ◽  
Vol 99 (8) ◽  
pp. 4167-4173 ◽  
Author(s):  
Edwin E Martínez‐Leo ◽  
Armando M Martín‐Ortega ◽  
Juan J Acevedo‐Fernández ◽  
Rosa Moo‐Puc ◽  
Maira R Segura‐Campos
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Mucuna Pruriens ◽  
Hela Cell Line ◽  
Vitro Effect

Anticancer and cytotoxic effects of troxerutin on HeLa cell line: an in-vitro model of cervical cancer

2020 ◽  
Vol 47 (8) ◽  
pp. 6135-6142
Author(s):  
Jalal Hassanshahi ◽  
Afsaneh Mirzahosseini-pourranjbar ◽  
Zahra Hajializadeh ◽  
Ayat Kaeidi
Keyword(s):  
Cervical Cancer ◽  
Hela Cell ◽  
Cell Line ◽  
In Vitro Model ◽  
Hela Cell Line ◽  
Cytotoxic Effects ◽  
Vitro Model

Heteroleptic Pd(II) dithiocarbamates: synthesis, characterization, packing and in vitro anticancer activity against HeLa cell line

2015 ◽  
Vol 68 (14) ◽  
pp. 2539-2551 ◽  
Author(s):  
Shahan zeb Khan ◽  
Muhammad Kashif Amir ◽  
Muhammad Moazzam Naseer ◽  
Rashda Abbasi ◽  
Kehkashan Mazhar ◽  
...  
Keyword(s):  
Hela Cell ◽  
Anticancer Activity ◽  
Cell Line ◽  
Hela Cell Line

scholarly journals Aktivitas Antiproliferatif Ekstrak Wasbensin Daun Eupatorium riparium Reg. : Studi In Vitro Pada HeLa Cell lin

2014 ◽  
Vol 18 (1) ◽  
Author(s):  
Linus Yhani Chrystomo ◽  
L. Hartanto Nugroho ◽  
Subagus Wahyuono ◽  
Aditya Krishar Karim ◽  
Kumiko Terada ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cell Line ◽  
Hela Cell Line

Eupatorium riparium Reg. adalah tumbuhan obat penting asli dari Mexico dan India Barat, yang masuk ke tanah Jawa sejak tahun 1800. Tumbuhan ini mempunyai catatan sejarah digunakan untuk obat tradisional dalam berbagai kultur budaya bangsa secara luas di seluruh dunia dan biasa digunakan untuk obat hipertensi, gagal jantung, diuretik, antikanker, antifungi, dan penyakit yang disebabkan oleh bakteri. Studi pada HeLa cell line ini dilakukan selama satu bulan. Selanjutnya, studi ini bertujuan meneliti aktivitas antiproliferatif ekstrak wasbensin daun E. riparium terhadap kanker servik manusia Hela cell line. Aktivitas antiproliferatif diuji menggunakan reagen proliferatif sel WST-1 dengan waktu 1, 2, dan 4 jam setelah diinkubasi selama 72 jam pada suhu 37oC dan 5%CO2. Hasil penelitian menunjukkan bahwa ekstrak wasbensin daun E. riparium mempunyai aktivitas proliferatif yang potensial terhadap HeLa cell line dengan nilai IC50 berikut 102.69 𝜇g/ml (1 jam), 198.67 𝜇g/ml (2 jam). Saran selanjutnya, penelitian lanjutan perlu dilakukan untuk mengetahui mekanisme antikanker HeLa cell line.Kata kunci: Eupatorium riparium Reg, antiproliferatif, HeLa, WST-1

Sign in / Sign up

Export Citation Format

Share Document