Study on MR Imaging of Endothelial Progenitor Cells Labeled with the Complex of Super Paramagnetic Iron Oxide and Transfection

2013 ◽  
Vol 683 ◽  
pp. 885-888
Author(s):  
Na Chang ◽  
Jun Zhang ◽  
Jun Shi Zhang
Keyword(s):  
Iron Oxide ◽  
Mr Imaging ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Mononuclear Cells ◽  
Gradient Centrifugation ◽  
Superparamagnetic Iron Oxide ◽  
Effective Rate ◽  
Endothelial Progenitor ◽  
Ficoll Density Gradient Centrifugation

To explore the characteristics of magnetic resonance（MR）imaging of the rat endothelial progenitor cells（EPCs）labeled with superparamagnetic iron oxide（SPIO）. Total mononuclear cells (MNCs) were isolated from SD rat peripheral blood by ficoll density gradient centrifugation, and then the cells were plated on fibronectin-coated culture dishes. Attached cells were collected after 7 days cultured. EPCs were indentified by the laser confocal microscope and were counted in the inverted fluorescence microscope. EPCs were incubated with Fe2O3-arginine for 24 h, and the cells underwent MR imaging with three sequences (T1 WI, T2 WI, T2*WI). The results showed that the effective rate of labeled EPCs was 96%, and the survival rate of cells was 95%. The signal intensity on MRI was significantly decreased in labeled EPCs compared with unlabeled cells. EPCs labeled with SPIO can be sensitively displayed by the MR imaging.

scholarly journals Exposure of Human Endothelial Progenitors to Sevoflurane Improves Their Survival Abilities

2016 ◽  
Vol 24 (2) ◽  
pp. 177-186
Author(s):  
Adelina Munteanu ◽  
Marilena Gilca ◽  
Gheorghita Isvoranu ◽  
Mihaela Surcel ◽  
Laura Ceafalan ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Cell Biology ◽  
Mononuclear Cells ◽  
Gradient Centrifugation ◽  
Human Umbilical Cord Blood ◽  
Tetrazolium Salt ◽  
Human Umbilical Cord ◽  
Endothelial Progenitor ◽  
Post Exposure

Abstract Endothelial progenitor cells (EPCs) have prominent roles in vessel and tissue repair; however, their regenerative efficacy is diminished due to the poor survival in the hostile microenvironment of the injured organs. Recent data suggest a promising potential of volatile anesthetics for improving stem cell biology. Thus, we hypothesized that exposure to sevoflurane could stimulate growth and viability of cultured EPCs. Total mononuclear cells were isolated from human umbilical cord blood by gradient centrifugation. After five days in culture, the cells were exposed for one or two hours to sevoflurane 2% or 4% in air/5% CO2, or only to air/5% CO2 (sham control) in a sealed modular chamber. 24 or 48 hours post-exposure, viability, proliferation and apoptosis were assessed using lactate dehydrogenase (LDH) leakage assay, a methyl tetrazolium salt (MTS) assay and FITC-annexin V/ propidium iodide (PI) staining, respectively. LDH leakage was discretely lowered, whereas the levels of formazan were significantly increased (p < 0.05 for 1 h incubation with 4% sevoflurane at 24 hrs post-exposure, and with 2% sevoflurane at 48 h post-exposure) in the preconditioned cultures, proving no cytotoxic effects and increased proliferation in treated cells versus control samples. Early (p < 0.05) and late apoptosis (p < 0.05 only for 2% sevoflurane) were diminished following the procedure. Thus, the commonly used sevoflurane anesthetic has protective effects on viability and proliferation of human early endothelial progenitor cells in vitro, suggesting a promising potential of anesthetic preconditioning for improving the regeneration of ischemic tissues.

scholarly journals Experimental study of endothelial progenitor cells labeled with superparamagnetic iron oxide in vitro

2014 ◽  
Vol 11 (5) ◽  
pp. 3814-3819 ◽  
Author(s):  
MENG-QI WEI ◽  
DI-DI WEN ◽  
XIAO-YING WANG ◽  
YI HUAN ◽  
YONG YANG ◽  
...  
Keyword(s):  
Experimental Study ◽  
Iron Oxide ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Superparamagnetic Iron Oxide ◽  
Endothelial Progenitor

scholarly journals Effects of adrenomedullin on the cell numbers and apoptosis of endothelial progenitor cells

2008 ◽  
Vol 31 (3) ◽  
pp. 117 ◽  
Author(s):  
Xiang-Quan Kong ◽  
Le-Xin Wang ◽  
Chuan-Sheng Yang ◽  
Shuang-Feng Chen ◽  
Yu-Zeng Xue ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Mononuclear Cells ◽  
Gradient Centrifugation ◽  
Clinical Importance ◽  
Dependent Manner ◽  
Endothelial Progenitor ◽  
Concentration Dependent Manner ◽  
Pi3k Inhibitor Ly294002 ◽  
Cell Numbers

Purpose: To investigate the effect of adrenomedullin on the cell numbers and apoptosis of endothelial progenitor cells (EPCs). Methods: Mononuclear cells were isolated from peripheral blood by Ficoll density gradient centrifugation. The cells were stimulated with adrenomedullin, before and after the treatment of adrenomedullin-receptor antagonist, adrenomedullin 22-52, or a PI3K inhibitor LY294002. Results: Adrenomedullin dose-dependently increased the number of EPCs (P < 0.05). Adrenomedullin also significantly decreased apoptosis rate of EPCs in a concentration-dependent manner (P < 0.05). In the isolated human mononuclear cells pretreated with adrenomedullin 22-52 or LY294002, adrenomedullin failed to increase the number of EPCs or to reduce the level of apoptosis. Conclusions: Adrenomedullin increases the number of EPCs and decreases their apoptosis. These actions are likely mediated by PI3K signaling pathways. The clinical importance of these favourable effects on EPCs remains to be determined.

Sign in / Sign up

Export Citation Format

Share Document