Determination of Functional Components of Peanut Skins Pigment

The pigments in black and red peanut skins were comparative analyzed in this paper. The pigments were extracted with ethanol solution, purified by AB-8 macroporous resin, and black peanut skins pigment (BPSP) was separated by ethyl acetate to obtain different compositions. The compositions of pigments were qualitatively analyzed by special chemical reaction of organics and UV-visible spectrophotometry. The results show that the compositions of BPSP are mainly anthocyanins and proanthocyanidins, and the contents are 9.39% and 16.62%, respectively. The proanthocyanidins are found in red peanut skins pigment (RPSP), and the content is 24.51%, and no anthocyanin is checked out. It is convenient, rapid, economical and effective for special chemical reaction of organics and UV-visible spectrophotometry, and it is suitable for preliminary identification of natural organics structure.

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Design of a novel optical sensor for determination of trace amounts of copper by UV–visible spectrophotometry in real samples

Applied Organometallic Chemistry ◽

10.1002/aoc.4110 ◽

2017 ◽

Vol 32 (3) ◽

pp. e4110

Author(s):

Eslam Pourbasheer ◽

Somayeh Morsali ◽

Zhila Azari ◽

Mohammad Ali Karimi ◽

Mohammad Reza Ganjali

Keyword(s):

Optical Sensor ◽

Real Samples ◽

Visible Spectrophotometry ◽

Uv Visible

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Cloud point extraction for the determination of copper (II) by UV-visible spectrophotometry

10.1063/5.0066811 ◽

2022 ◽

Author(s):

Ahmed Abd Ali Hassoni ◽

Ahmed S. Abbas

Keyword(s):

Cloud Point ◽

Cloud Point Extraction ◽

Visible Spectrophotometry ◽

Uv Visible ◽

Determination Of Copper

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DETERMINATION OF THE ANTIOXIDANT ACTIVITY OF PRASMAN LEAF EXTRACTS (AYAPANA TRIPLINERVIS [VAHL]) AND THE TOTAL FLAVONOID AND PHENOL CONTENTS OF THE MOST ACTIVE EXTRACTS

International Journal of Applied Pharmaceutics ◽

10.22159/ijap.2020.v12s1.ff021 ◽

2020 ◽

pp. 107-111

Author(s):

YUNITRIANTI ◽

BERNA ELYA ◽

ARIKADIA NOVIANI

Keyword(s):

Antioxidant Activity ◽

Ethyl Acetate ◽

Methanol Extract ◽

Total Flavonoid ◽

Dpph Assay ◽

Frap Assay ◽

Methanol Extracts ◽

Uv Visible ◽

Antioxidant Effects

Objective: Prasman (Ayapana triplinervis [Vahl]) is a plant that can eliminate free radicals through its antioxidant effects. However, little research hasbeen conducted to explore the antioxidant activity of the plant.Methods: A. triplinervis (Vahl) leaves, which were determined by the Indonesian Institute of Sciences (LIPI) Bogor, were used in this study. Meanwhile,extraction was performed using n-hexane, ethyl acetate, and methanol as the solvents. Based on the 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay usinga UV–visible spectrophotometer and based on ferric reducing antioxidant power (FRAP) assay using a microplate reader.Results: Based on the DPPH assay using a UV–visible spectrophotometer, n-hexane, ethyl acetate, and methanol extracts with a final concentrationof 25 μg/mL inhibited DPPH radical production by 38.91, 51.03, and 54.06%, respectively. Using the percent inhibition, the IC50 for the ethyl acetateand methanol extracts were 28.71 and 23.472 μg/mL, respectively. Based on FRAP assay using a microplate reader, the n-hexane, ethyl acetate, andmethanol extracts had ferrous equivalent antioxidant capacity values of 460, 828.99, and 940.22 μmol/g, respectively. The methanol extract had thegreatest antioxidant activity. The ethyl acetate and methanol extracts at the initial concentrations contained total phenol levels of 12.06 and 42.11 mgGAE/g extract, respectively, as well as total flavonoid levels of 3.24 and 3.41 mg QE/g extract, respectively.Conclusion: Based on the determination of phenol and flavonoid levels, the methanol extract had the greatest antioxidant effects.

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