Simultaneous Separation and Determination of Aconitine Compounds in Radix aconiti praeparata by High Performance Capillary Zone Electrophoresis

2014 ◽  
Vol 884-885 ◽  
pp. 522-525
Author(s):  
Su Ya Gao ◽  
Li Wang ◽  
Yun Zhang ◽  
Hua Li
Keyword(s):  
Capillary Zone Electrophoresis ◽  
High Performance ◽  
Uv Detection ◽  
Beta Cyclodextrin ◽  
Simultaneous Separation ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Radix Aconiti ◽  
Optimized Conditions

A simple and inexpensive high performance capillary zone electrophoresis (CZE) was applied to separate and determine three alkaloids in Radix Aconiti Praeparata simultaneously. The optimized conditions for separation were obtained using 20 mmol/L borax buffer (pH 9.0) containing 2 mmol/L beta-cyclodextrin (β-CD) and 20% methanol. Online UV detection was performed at 235 nm. A voltage of 16 kV was carried at 25°C. Injection was performed at 0.5psi for 10 s. The separation and determination were satisfactory and quick.

Determination of puerarin in pharmaceutical and biological samples by capillary zone electrophoresis with UV detection

Talanta ◽  
2012 ◽  
Vol 91 ◽  
pp. 83-87 ◽  
Author(s):  
Lizhen Liu ◽  
Feng Feng ◽  
Shaomin Shuang ◽  
Yunfeng Bai ◽  
Martin M.F. Choi
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Biological Samples ◽  
Uv Detection ◽  
Zone Electrophoresis ◽  
Capillary Zone

Separation and Determination of Triclosan and Bisphenol A in Water, Beverage, and Urine Samples by Dispersive Liquid–Liquid Microextraction Combined with Capillary Zone Electrophoresis–UV Detection

2013 ◽  
Vol 96 (2) ◽  
pp. 459-465 ◽  
Author(s):  
Huili Wang ◽  
Aina Zhang ◽  
Wenwei Wang ◽  
Minghua Zhang ◽  
Huanqiang Liu ◽  
...  
Keyword(s):  
Bisphenol A ◽  
Capillary Zone Electrophoresis ◽  
Uv Detection ◽  
Extraction Time ◽  
Urine Samples ◽  
Zone Electrophoresis ◽  
Capillary Zone ◽  
Dispersive Liquid Liquid Microextraction ◽  
Liquid Microextraction

Abstract Dispersive liquid–liquid microextraction combined with capillary zone electrophoresis–UV detection was developed for analyzing triclosan (TCS) and bisphenol A (BPA) in water, beverage, and urine samples. The factors influencing microextraction efficiencies, such as the kind and volume of extraction and dispersive solvent, the extraction time, and the salt effect, were optimized. A background electrolyte composed of 8 mM sodium tetraborate at pH 9.8 was used as the running buffer. Detection was performed at 214 nm. Under the optimum conditions (sample volume, 5.0 mL; extraction solvent, tetrachloroethane, 22.0 μL; dispersive solvent, tetrahydrofuran, 1.0 mL; extraction time, fewer than 5 s; and without salt addition), the enrichment factors were 110.2 and 82.0 for TCS and BPA, respectively. The linear range was 0.02–2 μg/mL with correlation coefficients of 0.9966–0.9969. LODs were in the range of 4.0–8.0 ng/mL. The environmental water, beverage, and urine samples (at spiking levels of 0.1 and 0.4 μg/mL) were successfully analyzed by the proposed method; the recoveries compared to previous methods were in the range of 81.2–103.3%. As a result, this method can be successfully applied for the rapid and convenient determination of TCS and BPA in water, beverage, and urine samples.

Determination of the Fungicide Validamycin A by Capillary Zone Electrophoresis with Indirect UV Detection

2003 ◽  
Vol 51 (26) ◽  
pp. 7523-7527 ◽  
Author(s):  
Jin He ◽  
Shou-Wen Chen ◽  
Li-Fang Ruan ◽  
Li-Li Cao ◽  
Jun Yao ◽  
...  
Keyword(s):  
Capillary Zone Electrophoresis ◽  
Uv Detection ◽  
Indirect Uv Detection ◽  
Validamycin A ◽  
Zone Electrophoresis ◽  
Capillary Zone
Sign in / Sign up

Export Citation Format

Share Document