Tumor Targeting of Plasmid DNA by Dextran Conjugation Based on Metal Coordination

2005 ◽  
Vol 288-289 ◽  
pp. 109-112
Author(s):  
H. Hosseinkhani ◽  
T. Aoyama ◽  
O. Ogawa ◽  
Yasuhiko Tabata
Keyword(s):  
Gene Expression ◽  
Zeta Potential ◽  
Plasmid Dna ◽  
Tumor Targeting ◽  
Metal Coordination ◽  
Hydroxyl Groups ◽  
Negative Zeta Potential

Tumor targeting of plasmid DNA was achieved through the conjugation of dextran derivative with chelate residue based on metal coordination. Spermine (Sm) was chemically introduced to the hydroxyl groups of dextran to obtain dextran-Sm derivative. A negative zeta potential of plasmid DNA became almost 0 mV by the Zn2+-coordinated conjugation with the dextran-Sm When the dextran-Sm-plasmid DNA conjugate with Zn2+ coordination was intravenously injected to mice subcutaneously bearing Meth-AR-1 fibrosarcoma, the dextran- Sm-plasmid DNA conjugate significantly enhanced the level of gene expression in the tumor, in contrast to free plasmid DNA..

scholarly journals Development of Highly Efficient, Glassy Carbon Foam Supported, Palladium Catalysts for Hydrogenation of Nitrobenzene

Nanomaterials ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1172
Author(s):  
Ádám Prekob ◽  
Mahitha Udayakumar ◽  
Gábor Karacs ◽  
Ferenc Kristály ◽  
Gábor Muránszky ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Glassy Carbon ◽  
Palladium Nanoparticles ◽  
Palladium Catalysts ◽  
Sucrose Solution ◽  
Supported Catalyst ◽  
Carbon Foam ◽  
Hydroxyl Groups ◽  
Polyurethane Elastomers ◽  
Oh Groups

Glassy carbon foam (GCF) catalyst supports were synthesized from waste polyurethane elastomers by impregnating them in sucrose solution followed by pyrolysis and activation (AC) using N2 and CO2 gas. The palladium nanoparticles were formed from Pd(NO3)2. The formed palladium nanoparticles are highly dispersive because the mean diameters are 8.0 ± 4.3 (Pd/GCF), 7.6 ± 4.2 (Pd/GCF-AC1) and 4.4 ± 1.6 nm (Pd/GCF-AC2). Oxidative post-treatment by CO2 of the supports resulted in the formation of hydroxyl groups on the GCF surfaces, leading to a decrease in zeta potential. The decreased zeta potential increased the wettability of the GCF supports. This, and the interactions between –OH groups and Pd ions, decreased the particle size of palladium. The catalysts were tested in the hydrogenation of nitrobenzene. The non-treated, glassy-carbon-supported catalyst (Pd/GCF) resulted in a 99.2% aniline yield at 293 K and 50 bar hydrogen pressure, but the reaction was slightly slower than other catalysts. The catalysts on the post-treated (activated) supports showed higher catalytic activity and the rate of hydrogenation was higher. The maximum attained aniline selectivities were 99.0% (Pd/GCF-AC1) at 293 K and 98.0% (Pd/GCF-AC2) at 323 K.

Linear forms of plasmid DNA are superior to supercoiled structures as active templates for gene expression in plant protoplasts

1988 ◽  
Vol 11 (4) ◽  
pp. 517-527 ◽  
Author(s):  
Nurit Ballas ◽  
Nehama Zakai ◽  
Devorah Friedberg ◽  
Abraham Loyter
Keyword(s):  
Gene Expression ◽  
Plasmid Dna ◽  
Plant Protoplasts ◽  
Linear Forms

Adsorption of Benzene from Aqueous Solution Using Base Modified Expanded Perlite

2012 ◽  
Vol 622-623 ◽  
pp. 1779-1783
Author(s):  
Richard Appiah-Ntiamoah ◽  
Xuan Thang Mai ◽  
Francis W.Y. Momade ◽  
Hern Kim
Keyword(s):  
Zeta Potential ◽  
Adsorption Capacity ◽  
Linear Regression Analysis ◽  
Basic Medium ◽  
Maximum Adsorption Capacity ◽  
Hydroxyl Groups ◽  
Expanded Perlite ◽  
Oh Groups ◽  
Base Solution ◽  
Ph Range

In this study, the adsorption capacity of expanded perlite (EP) for benzene at low concentrations in water was investigated after EP was treated with sodium hydroxide (NaOH). IR spectra used to characterize the modified EP showed that there was no bonding between NaOH and the hydroxyl groups on the surface of EP. However, the NaOH provided a basic medium for negatively charged surface oxide ions (-SO-) to form on EP. This fact was corroborated by pH readings of the modification solution. This reduced in pH from 10 to 9 at the end of the reaction which indicated that the hydroxyl OH- groups on the EP underwent deprotonation and hence releases H+ into the solution, and also positive sites on EP adsorbed OH- ions from the base solution. Mahir et al. in their paper Zeta potential of unexpanded and expanded perlite samples in various electrolyte media confirmed that EP has no isoelectric point and exhibits negative zeta potential in the pH range of 2-11. The surface oxides (-SO-) were believed to have given EP it adsorptive potential. Adsorption isotherm values correlated reasonably well with the Langmuir isotherm model and it parameters (qo and K) were obtained using linear regression analysis. A maximum adsorption capacity (qo) value of 19.42 mg/g was achieved.

Influence of Surface Zeta Potential on Adhesion of Chlorella sp. to Substratum Surfaces

2013 ◽  
Vol 690-693 ◽  
pp. 1431-1434
Author(s):  
Xin Ru Zhang ◽  
Ze Yi Jiang ◽  
Hao Yuan ◽  
Yuan Xiang Lu ◽  
Liang Chen ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Cell Surface ◽  
Mass Culture ◽  
Chlorella Sp ◽  
Microalgae Mass Culture ◽  
Negative Zeta Potential

Microalgae, in wet conditions, tend to grow on surfaces and form biofilms. The adhesion of microalgae to surfaces is very important for algal mass culture. The formation and development of microalgal biofims are in large denpend on the properties of cell surface, substratum surface and gowth medium. In this paper, the influence of substratum surface zeta potential on the microalgal biofilms was particularly investigated. We focused on a widely-used microalgal strain, the freshwater autotrophicChlorella sp..The adhesion phenomena ofChlorella sp.to surfaces with different zeta potential were observed microscopically. It was found thatChlorella sp.adhered easily to the surface with a positive zeta potential and difficultly to the surface with a negative zeta potential. We concluded that the surface zeta potential had a greater influence on the adhesion of microalgal cells to substratum surfaces. Our findings have important implications for microalgae mass culture and harvesting.

scholarly journals Successful transfer of plasmid DNA intoin vitrocells transfected with an inorganic plasmid–Mg/Al-LDH nanobiocomposite material as a vector for gene expression

2008 ◽  
Vol 20 (4) ◽  
pp. 045602 ◽  
Author(s):  
Mas Jaffri Masarudin ◽  
Khatijah Yusoff ◽  
Raha Abdul Rahim ◽  
Mohd Zobir Hussein
Keyword(s):  
Gene Expression ◽  
Plasmid Dna ◽  
Successful Transfer

Enhanced growth and matrix production by human osteoblastic cells cultured on biomaterials with negative zeta potential

Bone ◽  
2009 ◽  
Vol 44 ◽  
pp. S259 ◽  
Author(s):  
J.P. Dillon ◽  
P.J.M. Wilson ◽  
W.D. Fraser ◽  
B.K. Mwaura ◽  
M.J. Hayton ◽  
...  
Keyword(s):  
Zeta Potential ◽  
Osteoblastic Cells ◽  
Matrix Production ◽  
Human Osteoblastic Cells ◽  
Negative Zeta Potential ◽  
Cells Cultured
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